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Citations for Electron Microscopy Sciences :
251 - 300 of 316 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Cells were fixed for 20 min with a mixture of prewarmed (37°C) 0.25% glutaraldehyde (Electron Microscopy Sciences 16220) and 4% formaldehyde (Electron Microscopy Sciences 15714 ...
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bioRxiv - Neuroscience 2022Quote: ... Ribbons of 15-20 ultrathin (70 nm) serial sections were cut using a Diatome diamond knife (Electron Microscopy Sciences), mounted on Formvar-coated slot grids (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed and fixed for 20 min with 4% paraformaldehyde (PFA) in PBS (157-4, Electron Microscopy Sciences). For immunofluorescent detection and click chemistry ...
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bioRxiv - Neuroscience 2023Quote: ... Then the methanol was gradually replaced with Lowicryl HM-20 resin (monostep embedding kit, Electron Microscopy Sciences, cat# 14345): 50% Lowicryl (2 hours) ...
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bioRxiv - Plant Biology 2023Quote: ... A total of 20 μL mix was incubated on glow-discharged carbon-coated copper EM grids (300 mesh, EMS). Filter paper was used to remove any excess solution ...
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bioRxiv - Neuroscience 2019Quote: ... Dissected larvae were fixed for 20 (VNC) or 45 minutes (NMJs) in 4% formaldehyde solution (Electron Microscopy Sciences, Hatfield, PA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ul of the experimental solutions were incubated on glow-discharged to carbon-coated copper EM grids (300 mesh, EMS). Filter paper was used to remove any excess solution and the EM samples were then washed three times with swelling buffer ...
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bioRxiv - Molecular Biology 2021Quote: Samples for SFM imaging were prepared by depositing 20 μl reaction volume on a freshly cleaved mica (Muscovite mica, V5 quality, EMS) for 2 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The floor of the imaging chamber was a rectangular coverslip (22 × 40 mm, thickness 0.08-0.13 mm, No. 0, 72198-20; Electron Microscopy Sciences). The chamber was fitted onto a platform with solution inlets (PH-1 ...
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bioRxiv - Neuroscience 2020Quote: ... we immersed slides from −80 °C instantly into freshly made 4 % PFA (10mL vials of 20 % PFA; Electron Microscopy Sciences) in PBS for 30 mins at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... EVs were fixed by positioning the grid on top of a 20 µL drop of 2% paraformaldehyde (EMS, 157-4) for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Cell Biology 2020Quote: ... image positions were recorded and slides were washed in 1xPBS/0.2% Tween 20 to remove the mounting medium and re-fixed in 4% paraformaldehyde (Electron Microscopy Sciences) prior to DNA FISH experiment ...
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bioRxiv - Neuroscience 2020Quote: ... 20 mM NaCl and incubated with secondary 15 nm gold-tagged goat-anti-rabbit IgG F(ab’)2 (Electron Microscopy Sciences) diluted 1:10 in 20 mM Tris pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Copper grids containing thin sections were post-stained for 20 min in 2% aqueous uranyl acetate (Electron Microscopy Sciences; Cat #22400) and for 5 min in Reynolds lead citrate (Reynolds ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Developmental Biology 2023Quote: ... infiltrated with Embed-812 resin series by 20% increments in acetone (each step was 1 h) and embedded in Embed-812 (EMS, USA). After polymerization of the resin at 60°C for 36 h ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
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bioRxiv - Bioengineering 2023Quote: ... 20 uL of BEV sample was fixed in 2% EM-grade paraformaldehyde (157-4-100; Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with treatments for 20 min at 37°C and immediately fixed by addition of paraformaldehyde (Electron Microscopy Sciences) to 3.7% ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of purified proteins or immune complexes (∼0.02 mg/mL) were applied onto glow discharged (20 mA for 30s) carbon-coated 400 mesh copper grids (Electron Microscopy Sciences). After 5 s ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was washed with MQ water and stained for 20 min with 10 μl 0.1% uranyl formate solution (Electron Microscopy Sciences). The uranyl formate solution was prepared freshly and filtered with a 0.22 μm sterile syringe filter (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Slides were subsequently washed three more times for 5 minutes by immersion in 0.1% Tween-20 in PBS and mounted in EMS Shield Mount with DABCO (Electron Microscopy Sciences). Imaging was performed using a Carl Zeiss LSM710 confocal microscope at the microscopy imaging center (MIC ...
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bioRxiv - Neuroscience 2023Quote: ... the slides were mounted using MS Shield Mounting Medium With 4,6- diamidino-2-phenylindole (DAPI) and DABCO™ (EMS #17989-20).
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bioRxiv - Neuroscience 2023Quote: ... Brains were sliced using a cryostat into 16um sections and adhered onto super-frost plus slides (Electron Microscopy Sciences, 71869-20). Slides were kept on dry ice during slicing and then promptly stored at -80°C in a sealed box until use ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG/IgM-6nm gold secondary antibody (EMS) diluted 1:25 in 1% BSA PBS was added for 1.5 hours followed by another washing step ...
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bioRxiv - Microbiology 2022Quote: ... grids were washed by contact with 2 drops of milliQ water and were negatively stained with 20 µl of 1% uranyl acetate (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Genomics 2022Quote: ... biological specimens were transferred to microporous specimen capsules (30 μm porosity, 12 mm diameter, 11 mm high, ref #70187-20, Electron Microscopy Science) and dehydrated in a graded series of ethanol in double-distilled water (50 ...
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bioRxiv - Neuroscience 2020Quote: The injection site was cryo-sectioned to 20 μm sections and the sections were mounted onto Superfrost Plus Gold slides (Electron Microscopy Sciences) using NOA81 glue and a home-made tape-transfer system49 ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... of 0.01 and cultured in 6-well plates for 48 h with cMEM or cells from ETAs of COVID-19-infected patients were fixed with a freshly prepared 2% or 4% PFA solution (20% stock diluted in 1X HBSS; Electron Microscopy Sciences) or a 1.6X BD FACS™ Lysis Solution (1:6 in sterile dH2O ...
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bioRxiv - Microbiology 2021Quote: ... samples were dehydrated through a graded ethanol series (20%, 40%, 60%, 80%, 90%, 100%, 100%, 100%) followed by infiltration with an Embed-812 epoxy resin (Electron Microscopy Sciences):acetone series of 1:3 for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The free face was then coated with carbon (20 nm thickness) generated from a high purity carbon cord source (Electron Microscopy Sciences) using a Quorum Q150R ES sputter coater (Quorum Technologies ...
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bioRxiv - Plant Biology 2023Quote: Samples of Arabidopsis tissues were dehydrated in a successive ethanol series (20%, 40%, 60%, 80%, 95%, and 100%) and embedded in LR White resin (Electron Microscopy Sciences ). Thin sections (1 µm ...
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bioRxiv - Bioengineering 2023Quote: A 80 × 50 × 20 mm (L × W × T) block of PMMA (Acrylic Plastic 24210-08, Electron Microscopy Sciences, Hatfield, PA 19440, USA) was cast and cured in a mold for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: The TEM samples were prepared on glow-charged (20 s oxygen plasma flash) Formvar carbon-coated copper grids (FCF400-Cu, Electron Microscopy Sciences) according to the protocol previously described by Castro et al ...
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bioRxiv - Cell Biology 2023Quote: ... The hindlimbs were then submerged in a 100 mL glass beaker filled with 20 mL of Karnovsky fixative [71] ((2.5% EM-grade glutaraldehyde (Electron Microscopy Sciences #16019) plus 2.0% EM-grade paraformaldehyde (Electron Microscopy Sciences #15714-S ...
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bioRxiv - Molecular Biology 2023Quote: ... the RPE flat-mount was washed three times with PBS (20 min per wash) and mounted in Fluoro-Gel (Electron Microscopy Sciences) solution ...
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bioRxiv - Immunology 2024Quote: ... slides were dried at room temperature for 20 min and each section on the slide was outlined with hydrophobic barrier with Pap Pen (Electron Microscopy Sciences) and fixed with 4% PFA at RT for 10min ...
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bioRxiv - Neuroscience 2024Quote: ... mice were perfused with phosphate-buffered saline (PBS, warmed up to 37 °C) with heparin solution (20 units / mL) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science) in PBS ...
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bioRxiv - Microbiology 2022Quote: The cells from the one-step growth experiment were immobilized by spotting 20 μl of fixed cell suspension on SuperFrost Plus glass slides (Electron Microscopy Sciences, USA), on which silicone isolators (Grace Bio-Lab ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Cell Biology 2019Quote: ... incubated for 45 minutes with goat anti-rabbit 15nm gold antibody (EMS) diluted 1:20 in filtered blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... Girds were immersed in 15µl beads of secondary antibody (Electron microscopy sciences, goat-anti-rabbit 15 nm ...
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bioRxiv - Genomics 2021Quote: ... Slides were washed three times in 1x TBS/0.1% Tween-20 for 10 minutes before being incubated with 200 µL 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-487) in a dark humidity chamber at room temperature for 30 minutes ...
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bioRxiv - Physiology 2020Quote: ... at −20°C with a thickness of 10um through the mid-belly and mounted on SuperFrost glass slides (Electron Microscopy Sciences, catalog no. 71882-01). For analysis of fiber cross-sectional area (CSA) ...
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bioRxiv - Cell Biology 2023Quote: ... The cornea and the lens were removed and the eyecup was embedded in a mixture of two parts 20% sucrose in PB and one part optimal cutting temperature compound (Tissue Tek, Electron Microscopy Sciences, Hateld, PA, USA) and frozen at -80° with the dorsal/superior side up ...
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bioRxiv - Developmental Biology 2024Quote: ... before progressive dehydration in an ethanol series (30-50-70-80-90- 100%, at least 20 minutes per step) and chemical drying with hexamethyldisilazane (HMDS; Electron Microscopy Sciences 50-243-18). Dry samples were mounted on aluminum stubs using double-sided carbon tape and sputter-coated with gold-palladium before imaging on a Hitachi 3000N VP electronic microscope at 20kV.
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bioRxiv - Cell Biology 2019Quote: ... goat secondary antibodies conjugated with 6 nm and 10 nm gold particles (EMS) were applied to the sections on the grids ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used were 6 nm anti-mouse immunogold particles (Electron Microscopy Sciences). Negative staining was carried out as previously described [17] ...