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Citations for Electron Microscopy Sciences :
151 - 200 of 316 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... the supernatant was discarded and the pellet resuspended in primary fixating buffer (2% glutaraldehyde/0.1 M sodium cacodylate buffer, pH 7.4, Electron Microscopy Sciences) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed in 0.1 M cacodylate buffer (pH 7.4) with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed by immersion in Modified Karnovsky’s Fixative (2% Paraformaldehyde and 2.5% Glutaraldehyde in 0.1 M Sodium Cacodylate Buffer, pH 7.4 (Electron Microscopy Sciences (Hatfield, PA), #15960-01) ...
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bioRxiv - Cell Biology 2020Quote: ... and infiltrated with Monostep Lowicryl HM-20 (Electron Microscopy Sciences, Hatfield, PA) in increasing concentrations of 1:1 ...
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bioRxiv - Neuroscience 2021Quote: ... Embryos were fixed in 4% paraformaldehyde (PFA) (20% stock; EMS, Hatfield, PA) in artificial sea water overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and processed and embedded in Lowicryl HM-20 resin (Electron Microscopy Sciences) in a Leica AFS freeze-substitution instrument ...
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bioRxiv - Neuroscience 2022Quote: ... fixed for 20 minutes in PFA (4%, Electron Microscopy Sciences, 15714-S) plus Sucrose (4% ...
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bioRxiv - Genetics 2020Quote: ... Ovaries were fixed for 20 minutes in 3.7% paraformaldehyde (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... fixed for 20 minutes in PFA (4%, Electron Microscopy Sciences, 15714-S) plus Sucrose (4% ...
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bioRxiv - Microbiology 2023Quote: ... and embedded progressively at -20°C in LR Gold resin (EMS, USA). Resin polymerization was carried out at -20°C for 7 days under UV illumination ...
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2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neuronsbioRxiv - Neuroscience 2024Quote: ... followed by 20 mL ice-cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on bone slices for 3 days were unroofed as described above and fixed for 10 min in a 0.2M sodium cacodylate buffer (pH 7.4) containing 2% paraformaldehyde (Electron Microscopy Sciences 15710) and 2.5% glutaraldehyde (Electron Microscopy Sciences 16220) ...
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bioRxiv - Plant Biology 2021Quote: ... The roots of 7 to 10 day old seedlings were collected and fixed for 30 min with 2-(N-morpholino) ethane sulfonic acid (MES) buffer solution pH 5.7 containing 4% paraformaldehyde (32% methanol-free solution; Electron microscopy Sciences). pH was adjusted to 5.7 with KOH solution.
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bioRxiv - Systems Biology 2021Quote: ... Immunofluorescence was performed via heat-mediated antigen retrieval with a Tris-EDTA buffer (10mM Tris, 1mM EDTA, pH 9.0) in a Retriever 2100 pressure cooker (Electron Microscopy Sciences). Slides were incubated overnight at 4°C with the following primary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... 2.5% in phosphate buffer (PB 0.1 M [pH 7.4]) for 1 h at RT and postfixed in a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA) with 1.5% of potassium ferrocyanide (Sigma ...
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bioRxiv - Biophysics 2022Quote: Inactivated CHIKV in HBS buffer (pH 7.5) was applied to lacey carbon grids with a thin continuous carbon film (400 mesh) (Electron Microscopy Sciences). The grids were glow discharged (negative charge ...
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bioRxiv - Systems Biology 2020Quote: ... rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Systems Biology 2020Quote: ... the La2-DAB solution was removed and cells were rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Neuroscience 2019Quote: 63 day old hMO specimens were immersion-fixed in a solution of 2 % PFA and 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences, Hatfield, PA) for 3 h ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... atala eye was immersed for prefixation in 2.5% Glutaraldehyde/2% paraformaldehyde in 0.1M Sodium Cacodylate buffer (pH 7.4) (Electron Microscopy Sciences, PA, USA) for 2h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 2.5% in phosphate buffer (PB 0.1 M [pH 7.4]) for 1 h at RT and postfixed in a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA) with 1.5% of potassium ferrocyanide (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4 buffer) was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were rinsed in 1x PHEM buffer (60mM PIPES, 25mM HEPES, 10mM EGTA, 2mM MgCl2) pH 6.8 and fixed in 4% formaldehyde (FA, #15710, EMS, Hatfield, USA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Microbiology 2023Quote: ... and Spk-M (buffer-exchanged into 50 mM Tris pH 7.5 and 100 mM NaCl) were applied on a carbon-coated copper grids (400 mesh, Electron Microscopy Sciences) using 5 μL of protein solution (10 μg/mL ...
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bioRxiv - Developmental Biology 2023Quote: Wing imaginal discs dissected from late third instar larvae in 1×PBS (pH 7.4) were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 40[minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... were fixed for 20 min at RT with 4% paraformaldehyde (Electron Microscopy Sciences) diluted in PBS (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... and then with 4% PFA (bought as 20% PFA from Electron Microscopy Sciences) in DPBS ...
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bioRxiv - Biochemistry 2022Quote: ... 20 s) copper grids with a thin carbon film (CF300-Cu-UL, EMS), washed with water ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 20 mL of 4% paraformaldehyde (Electron Microscopy Sciences, cat. no. 15714) in PBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Seeds were then treated with a 20 μM solution of ethylmethane sulfonate (EMS), an alkylating agent that induces G:C to A:T substitutions (Greene et al. ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Neuroscience 2021Quote: ... The animals were intracardially perfused with 100 mL of 0.9% physiological saline followed by approximately 250 mL of freshly prepared 0.1 M sodium cacodylate buffer (pH 7.4; Electron Microscopy Sciences cat #11653), which contained 2.5% glutaraldehyde (Electron Microscopy Sciences cat #16320 ...
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bioRxiv - Microbiology 2020Quote: ... infected cells were washed twice with PBS buffer (pH 7.4) and fixed with a 4% methanol-free paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS buffer (pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was lysed until no visible pieces remained just prior to use in 15 μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) with a pestle (Electron Microscopy Sciences, 64788) and a LabGen 7B homogenizer (ColePalmer ...
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bioRxiv - Physiology 2022Quote: ... freshly isolated pituitary glands were fixed with 2.5% formaldehyde/glutaraldehyde in 0.1 M Sodium Cacodylate Buffer (pH 7.4, Emsdiasum) and 1% OsO4 (Electron Microscopy Science, Cat No. 15949), followed by dehydration and staining with uranyl acetate/lead citrate ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cells were first fixed with 1 mL of the fixative (2% paraformaldehyde [Electron Microscopy Sciences] dissolved in 250 mM HEPES [pH 7.4]) for 10 min at room temperature (approximately 25°C) ...
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bioRxiv - Cell Biology 2020Quote: ... cell cultures were fixed for 20 minutes in 4% paraformaldehyde (15710, Electron Microscopy Sciences) at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: Gastrulae and larvae were fixed in 4% paraformaldehyde (PFA) (20% stock; EMS, Hatfield, PA) in FSW overnight at 4°C (1D5 ...
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bioRxiv - Molecular Biology 2020Quote: ... then stained with a 1:20 dilution of Giemsa staining solution (Electron Microscopy Sciences). Stained cells were imaged using an Evos FL Imaging System (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... labeled samples on 20 mm coverslips thickness #1.5 (Electron Microscopy Sciences, Hatfield, PA, USA) were post-fixed in 2% PFA (5 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: RAW 264.7 cells were seeded at 20% confluence on #1.5 coverglass (Electron Microscopy Sciences) the day prior to phagosome fragmentation ...
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bioRxiv - Neuroscience 2023Quote: ... at −20°C using biopsy pens EMS Rapid-Core (Electron Microscopy Sciences, Hatfield, USA). To localize the subregions of interest in the brain ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then transferred to a 1% paraformaldehyde (20% PFA, Electron Microscopy Sciences, #15713) in S2 solution and fixed overnight at 4°C while rotating ...
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bioRxiv - Microbiology 2019Quote: Equal numbers of bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29 ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Neuroscience 2020Quote: ... the appropriate plane of anesthesia was checked by a lack of toe pinch reflex and the animals were transcardially perfused with 15 ml 0.15 M cacodylate buffer (EMS, Hatfield, PA, pH 7.4) followed by 30 ml fixative mixture containing 0.08 M cacodylate (pH 7.4) ...