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Citations for Electron Microscopy Sciences :
2751 - 2800 of 2809 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... rats were transcardially perfused with an isotonic solution at 9.25% in distilled water as a prewash and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion Two TM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Genomics 2023Quote: ... cells in the 6 well glass bottom plates were fixed by aspirating the medium and replacing it with 4% paraformaldehyde (Electron Microscopy Sciences, 15710-S) in a 1x RNase free PBS solution made from 10x RNase Free PBS (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... Grids were made using a Vitrobot™ Mark IV (FEI) at 4 °C and 100 % humidity with Grade 595 Vitrobot™ filter paper (Electron Microscopy Sciences). All grids were plunge frozen using liquid ethane and stored in liquid nitrogen.
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bioRxiv - Developmental Biology 2023Quote: Gels were fixed by 30 minutes rotation incubation at room temperature in freshly prepared 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA, Cat# 15710S) diluted in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... The mammary gland was excised and fixed overnight from either lactating wild-type (C57BL/6) or oxytocin knockout mice in 4% PFA (Electron Microscopy Sciences, Hatfield, PA). Tissue was incubated at 37 °C in CUBIC Reagent 1 A (10 wt% Triton ...
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bioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with ketamine/xylazine and transcardially perfused with PBS followed by PFA (4% in PBS; Electron Microscopy Sciences, cat n°15700). Extracted brains were post-fixed (PFA 4% overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and pancreas were harvested at the experimental endpoint and immediately fixed in 4% paraformaldehyde in 1X PBS (16% solution, Electron Microscopy Sciences, cat# 15711) at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... or any of the four possible distributions at the 4-cell stage (ABa and P2; ABa and EMS; ABp and P2; ABp and EMS), were more likely to harbor sperm-contributed centrioles using the χ2 test as implemented in the library SciPy ([https://scipy.org/citing-scipy/]) ...
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bioRxiv - Neuroscience 2024Quote: ... 240 mg/kg body weight) or on ice (for early postnatal animals) and transcardially perfused with PBS followed by ice-cold 4% paraformaldehyde (EMS #15714, diluted in PBS). Spinal cords were removed and post-fixed for 2 hours (neonatal spinal cords ...
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bioRxiv - Neuroscience 2024Quote: ... animals were anesthetized in 0.02% MS-222 and then immersed in 4% PFA (diluted from 16% PFA in 1xPBS, Cat # 15710, Electron Microscopy Sciences, Fort Washington, PA) using a Pelco Biowave Pro microwave (Model 36700 ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and then perfused transcardially with room temperature (RT) phosphate buffered saline (PBS) with heparin (0.02 mg/mL) and then ice-cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, catalog no: 15714). Brains were dissected and post-fixed in 4% PFA overnight at 4°C and cryopreserved in 30% sucrose in PBS for ∼2 days at 4°C ...
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bioRxiv - Physiology 2024Quote: ... The sections were cover-slipped in Fluoromount-G™ Slide Mounting Medium with DAPI (4′,6- diamidino-2-phenylindole; Electron Microscopy Sciences, Hatfield, PA) and examined under a brightfield microscope to identify irregularities in the luminal surface in eTNAP mice ...
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bioRxiv - Cell Biology 2024Quote: ... fixed for 20 min in 4% EM-grade paraformaldehyde (PFA) diluted in phosphate-buffered saline (PBS) from an 8% PFA Aqueous Solution (Electron Microscopy Sciences, 157-8) and blocked for 1 h in PBS plus 0.2% Triton X-100 and 4% normal goat serum (NGS ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were diluted 10-fold in liposome buffer and 5 μL was applied to glow-discharged 400 mesh copper grids coated with formvar/carbon film (Electron Microscopy Sciences). After 1 min ...
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bioRxiv - Bioengineering 2021Quote: ... ∼5 μL of each sEV-particle complex was left to adhere onto formvar carbon coated copper Grid 200 mesh (Electron Microscopy Sciences) for 5 mins followed by 5 mins of negative staining with 2% aqueous uranyl acetate ...
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bioRxiv - Neuroscience 2020Quote: ... Each coverslip was mounted on a stub with carbon tape and samples were sputter coated with iridium at 10μA to a thickness of approximately 5 nm (EMS model 150T S). The coverslips were then examined on a Hitachi S4300 SEM (Hitachi High Technologies America Inc. ...
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bioRxiv - Bioengineering 2022Quote: ... We applied 5 μL RBC-EV sample to a 200 mesh copper grid coated with formvar carbon film (FCF200-Cu, Electron Microscopy Sciences) for 30 seconds ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 µL sample was spotted onto a glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences CF300-Cu-50), incubated for two minutes then gently blotted onto filter paper to remove excess solution ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were then washed three times in PBS for 5 minutes each prior to cover slipping using Fluorogel mounting medium (Electron Microscopy Sciences).
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bioRxiv - Microbiology 2023Quote: ... and 150 mM NaCl buffer and 5 µL was applied to glow-discharged 400 mesh copper grids coated with formvar/carbon film (Electron Microscopy Sciences). Grids were blotted after 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were stained with 10% Uranyl acetate (Sisco Research Laboratory Pvt. Limited, 81405) for 5-min and Sato’s lead citrate (Electron Microscopy Sciences, 17800) stain for 1-min ...
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bioRxiv - Immunology 2024Quote: ... and 5% glycerol and applied to glow-discharged grid with continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences). The grids were stained with 2% uranyl formate (UF) ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of diluted phage solution was applied onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA, USA). Following a 3-minute incubation ...
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bioRxiv - Neuroscience 2021Quote: ... Brains from adult females were dissected in Phosphate-buffered saline (PBS, Eurobio, ref CS0PBS0108) and fixed for 15 min in 4% paraformaldehyde (Electron Microscopy Sciences, Cat # 15714-S) at room temperature (RT) ...
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bioRxiv - Genetics 2020Quote: ... Then the cells were counted and diluted to 106 cells/ml and fixed with 4% paraformaldehyde aqueous solution (Electron Microscopy Sciences #15710, Formaldehyde methanol-free) for 10 minutes at room temperature (RT) ...
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bioRxiv - Developmental Biology 2021Quote: ... The eye imaginal discs were dissected in cold 1X solution of Phosphate Buffered Saline (PBS) and fixed using 4% p-formaldehyde (Electron Microscopy Sciences, Catalog no. 15710). Fixed tissues were washed using PBS-T [0.2% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... the cultured neurons were fixed for 10-15 min by transferal of the coverslip into a dish with 4% paraformaldehyde (16% aqueous solution, 15710; Electron Microscopy Sciences, Hatfield, Pennsylvania, USA) and 0.2% glutaraldehyde (25% aqueous solution ...
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bioRxiv - Bioengineering 2020Quote: Animals were implanted with a stainless-steel probe or a Fiber F1/F2 for 4 weeks and then anesthetized with a ketamine (20 mg/mL)/xylazine (2 mg/mL) solution and transcardially perfused with phosphate-buffered saline (PBS) (Fisher BP661-10) followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Cat. #15714-S) in PBS ...
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bioRxiv - Genomics 2020Quote: ... cells were fixed by adding the fixative directly to the medium to a final concentration of 4% paraformaldehyde (PFA) (Electron Microscopy Sciences; cat. no. 15714) using the automated liquid handler BlueWasher (BLUE CAT BIO) ...
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bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Neuroscience 2023Quote: ... the coverslips were rinsed with 1X PBS and then fixed with freshly prepared 4% paraformaldehyde (from 32% Paraformaldehyde Solution, Electron Microscopy Science Cat# 15714-S) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... mice were placed under anesthesia and tissues were perfused with 10mL of cold PBS and 5mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, #15710, Hatfield, PA). Brains were post-fixed overnight in 4% PFA at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were stained en bloc with 2% aqueous uranyl acetate overnight at 4° C in the dark (22400-2, Electron Microscopy Sciences, Hatfield, PA, USA). After staining ...
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bioRxiv - Genetics 2024Quote: ... i3LMNs were cultured in clear-bottom imaging 96-well plates and fixed with an equivalent volume of 4% paraformaldehyde (PFA, Electron Microscopy Sciences 50-980-487) in PBS added directly to the cell culture media ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were rinsed three times with PBST for 5 min on ice and then embedded and frozen in Tissue Freezing Medium (Electron Microscopy Sciences, PA). Samples were sectioned (8μm ...
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bioRxiv - Biochemistry 2020Quote: End-point samples from wt α-syn aggregation assays (5 μL) were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 seconds using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg of the sample was placed onto a glow discharged 200-mesh formvar/carbon copper grid (Electron Microscopy Sciences, Hatfield Township, PA) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed three times for 5 min in PBS 1X and mounted using Fluoromont G fluorescence mounting medium (EMS, No. 17984-25). For all immunofluorescence analysis ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Neuroscience 2022Quote: ... The slice was then left to rest in the recording chamber for 4-5 minutes to ensure transport of biocytin to distal dendrites and axon processes (Swietek et al., 2016)39 before the slice was moved into 4% PFA (Electron Microscopy Sciences, CAS #50-00-0) diluted in 0.1M Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... coli BioParticles™ conjugate or DQ™ Green BSA was added for 30min at 4°C and moved to Cytoseal mounting medium (Electron Microscopy Sciences, Hatfield, PA) set at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: Subcutaneous tumors were excised from flank skin including adjacent skin layers and then fixed in 4% PFA (16% PFA, Electron Microscopy Sciences, diluted in 1X PBS) at 4°C overnight on a rotator ...
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bioRxiv - Immunology 2021Quote: 5 µl of the sample was deposited on a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences, catalog number FCF400-Cu-50), incubated for 1 min and blotted away ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Microbiology 2020Quote: ... The sample was then mounted on a SEM sample holder with double-sided carbon tape and sputter-coated with 5 nm of Pt/Pd (EMS 150 T S Metal Sputter Coater, Electron Microscopy Science, Hatfield, PA). Using the Zeiss SmartSEM software ...
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bioRxiv - Cell Biology 2021Quote: ... The sections are again washed thrice in PBS and stained with DAPI for 5 minutes and mounted with Fluoromount-G solution (17984-25, Electron Microscopy Sciences, Pennsylvania, USA). Using a Zeiss Axio Imager Z1 ApoTome Microscope ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were stained en bloc with 0.25% uranyl acetate then dehydrated in graded series of ethanol on ice followed by one wash with 100% ethanol and two washes with propylene oxide (5 min each) and embedded with EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Sections were cut at 60 nm on a Leica EM UC6 ultramicrotome ...
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bioRxiv - Biophysics 2024Quote: ... cells were imaged under physiological conditions (5% CO2 and 37°C) via a stage top incubator (Tokai Hit) with type 37 immersion oil (Electron Microscopy Sciences, #16914-01). Fixed cells were imaged at room temperature (22°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The vaccine powder was reconstituted and diluted 5 times with water and then transferred to a glow discharge-treated carbon coated copper grid from Electron Microscopy Sciences (Hatfield, PA). The sample was stained with a 2% uranyl acetate solution for 2 min and then air dried ...