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Citations for Electron Microscopy Sciences :
2651 - 2700 of 2809 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: RPE1 and U2OS cells were grown on poly-L-lysine coated coverslips and fixed in 4% paraformaldehyde (16% pfa (Electron Microscopy Sciences) diluted in 1x PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Corning)-coated coverslips and transfected according to cell culture protocol and fixed 48 hours after transfection with 4% paraformaldehyde (#15710, Electron Microscopy Sciences) in 1x DPBS for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the epidermal layer was carefully peeled away from the dermis using a S-shaped tweezer and were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in PBS on a nutator ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown on coverslips with the conditions described above and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 157-8-100) in 1X DPBS (Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... The leg fragments were then placed directly into 500 µl of 4% paraformaldehyde (20% EM grade stock solution, Electron Microscopy Sciences #15713) in filtered artificial seawater for 50 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
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bioRxiv - Immunology 2021Quote: For negative staining 5 µl of NLRP3 sample was placed on a copper grid (Electron Microscopy Sciences, cat. no: FCF400CU50), incubated for 1 min ...
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bioRxiv - Bioengineering 2021Quote: ... An aliquot (5 μl) of each sample was placed onto a CF300-Ni mesh Nickel grid (Electron Microscopy Sciences, EMS). The sample grid was deposited for 1 minute using 2% uranyl acetate solution ...
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bioRxiv - Bioengineering 2021Quote: ... An aliquot (5 μl) of each sample was placed onto a CF300-Ni mesh Nickel grid (Electron Microscopy Sciences, EMS). The sample grid was deposited for 1 minute using 2% uranyl acetate solution ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The sample was then mounted on a SEM sample holder with double-sided carbon tape and sputter-coated with 5 nm of Pt/Pd (EMS 150 T S Metal Sputter Coater ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: For collagen staining deparaffinized and re-hydrated slides were stained for 5 min in Van Gieson solution (EMS, 26374-06) followed by dehydration by 2 changes in 100% ethanol and cleared by 2 changes of xylenes ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of the endosomal suspension was spotted on a mica sheet (Muscovite Mica-V1, Electron Microscopy Sciences, Hatfield, PA) and dried for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μl of the mixed solution was dropped onto the 200 mesh formvar/carbon copper grid (Electron Microscopy Sciences, USA), allowing it to almost dry for 20 minutes ...
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bioRxiv - Microbiology 2024Quote: ... A 5 μl aliquot of the sample was deposited onto a 300 mesh copper grid coated with carbon film (EMS). After allowing the sample to interact with the grid for 60 seconds ...
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bioRxiv - Bioengineering 2023Quote: 5 μL of D-GQDs/exo loaded on the Poly-L-Lysine coated slides (63410; Electron Microscopy Sciences, PA, USA) and was fully covered with 18 mm × 18 mm square cover glasses (2845-18 ...
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bioRxiv - Cell Biology 2024Quote: ... The stock solution was first diluted in DMSO to make a 5 mM solution and then further diluted in perfusion media (EMS) to give the final concentration of 1 μM for drug treatment and 0.02% DMSO for the vehicle control ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl sample was applied to copper 400-mesh grids with an ultra-thin layer of carbon (Electron Microscopy Sciences, cat. No. CF400-Cu-UL), that had been glow-discharged in air for 30 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated overnight at 4°C in a 1:100 dilution of Goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8 nm colloidal gold (Aurion, Electron Microscopy Sciences, Fort Washington, PA, cat #25121-0.6ml). Following washes in IEM buffer ...
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bioRxiv - Cell Biology 2020Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocytes were washed with DPBS and then fixed with 4% paraformaldehyde (diluted from a 16% solution; Electron Microscopy Sciences cat. no. 15710) for 15 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence analysis of PGCLC bodies or rOvaries was performed on cryosections prepared as follows: aggregates were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Science, 15713) in PBS at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Developmental Biology 2021Quote: Day 11 IVDi tissues were treated while still attached to the transwell member as follow: culture medium was carefully removed from the transwell and the whole membrane was fixed in 4% PFA (Electron Microscopy Science, 15713) in PBS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was perfused quickly with cold 0.1 M PBS to clear blood before fixing the brain with cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, USA). Brains were post fixed in 4% PFA solution overnight (12-24 hr ...
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bioRxiv - Genetics 2022Quote: ... adult female flies were dissected in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 2 hours while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... The inner ears were dissected from P0 pups and E15.5 embryos directly into cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with wheat germ agglutinin for 10 min prior to fixing with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min and permeabilization with 0.5% Triton-X 100 for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were filleted on sylgard plates in room temperature (RT) PBS and fixed on a shaker in 4% paraformaldehyde (Electron Microscopy Sciences, EMS) in PBS for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... mice were transcardially perfused with 20 mL ice cold 1X DPBS followed by 20 mL 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA). Brains were extracted and post-fixed for at least 1 day in 4% PFA at 4°C and transferred to 30% sucrose (Millipore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... At that time cells were washed once with sterile 1x PBS and then fixed with 4% PFA solution (Electron Microscopy Sciences; Ref# 15710) for 10 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: Neural cell cultures were fixed post-live-cell fluorescent imaging using a solution of 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) + 8% w/v sucrose in 1× PBS for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of freshly purified peak fraction sample was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA), washed with deionized water and stained with freshly prepared 1.5% uranyl formate ...
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bioRxiv - Microbiology 2023Quote: Salivary glands were dissected out from wandering third instar larvae and fixed in PBS with 4% paraformaldehyde (EM Grade; Electron Microscopy Sciences, 15710) at room temperature for 20 min with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: Quiescent media was replaced every other day for 7 days and then adherent qNSCs were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in HBSS(-) for 30 min at room temperature (RT) and then immediately fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 1 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are washed with culture medium and fixed for 2 minutes with room temperature fixative (4% EM grade paraformaldehyde (Electron Microscopy Sciences #19202) + 0.1% EM grade glutaraldehyde (Ted Pella ...
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bioRxiv - Immunology 2022Quote: ... they were perfused with 40 mL of ice-cold PBS(-) and then with 30 mL of ice-cold 4% PFA (Electron Microscopy Science 15714) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized by intraperitoneal injection of 100 mg/kg ketamine /20 mg/kg xylazine (Anased) mix and transcardially perfused with PBS followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences 50980487). Brains were dissected and postfixed overnight in 4% PFA at 4°C ...