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Citations for Electron Microscopy Sciences :
2601 - 2650 of 2665 citations for 4' Bromo 2' fluoro 3 2 methoxyphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed for 1 hour at room temperature (RT) in Paraformaldehyde (PFA) 4% in PBS 1X (32% stock, Electron Microscopy Sciences, 15714). They were washed 4 times for 10min in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized by intraperitoneal injection of 100 mg/kg ketamine /20 mg/kg xylazine (Anased) mix and transcardially perfused with PBS followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences 50980487). Brains were dissected and postfixed overnight in 4% PFA at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Images of fixed cells were taken on either the LSM or spinning disk confocal microscopes at 40x after incubation in 4% paraformaldehyde (Electron Microscopy Sciences, #15710) for 5 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were initially washed with 1X Assay buffer (GOLGI-ID kit) and then fixed with 4% of 16% paraformaldehyde solution (Electron Microscopy Sciences, 15710) at room temperature for 10 minutes before staining the cells for detection of Golgi bodies using the manufacture’s manual for “Staining Adherent Fixed Cells” ...
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bioRxiv - Neuroscience 2024Quote: ... the 33 mice were deeply anesthetized with isoflurane and transcardially perfused with 10mL of ice-cold PBS followed by 10mL of 4% paraformaldehyde (16% PFA diluted in PBS; Electron Microscopy Sciences 15710). The olfactory bulbs (OBs ...
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bioRxiv - Neuroscience 2024Quote: At 10-15 days in vitro (DIV) coverslips with cells were washed with phosphate-buffered saline (PBS) and fixed in 4% paraformaldehyde (PARA) (Electron Microscopy Sciences, ON) in PBS for 10-15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... the tops of the wells were cleaned and cells on the bottom of the membrane were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, 15713) and stained with 10 μg/mL DAPI in 1% Triton X100 (Sigma T9284) ...
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bioRxiv - Developmental Biology 2024Quote: Bovine embryos and embryonic cells from different batches/days of experiments were fixed in freshly made 4% paraformaldehyde (Electron Microscopy Science, Hatfield, PA) at room temperature for 15 minutes followed by permeabilization in 1% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
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bioRxiv - Cell Biology 2020Quote: ... proliferating MuSC and C2C12 cells were cultured on pre-coated glass coverslips and then fixed in 4% paraformaldehyde/PBS (Electron Microscopy Sciences, Hatfield, PA). RNA hybridization and signal development were carried out using Basescope assay (Advanced Cell Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Cell Biology 2022Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Mounting media (~150 μL/slide) was prepared the day of use by mixing Tris-MWL 4-88 (Electron Microscopy Sciences #17977-150, Hatfield, PA) with AF300 (Electron Microscopy Sciences #17977-25 ...
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bioRxiv - Genomics 2021Quote: ... Slides were washed three times in 1x TBS/0.1% Tween-20 for 10 minutes before being incubated with 200 µL 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-487) in a dark humidity chamber at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were subjected to intracardiac perfusion with 5mL of PBS containing heparin followed by 20mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, RT 15714) in PBS ...
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bioRxiv - Genetics 2022Quote: ... samples were washed 3x in 0.3% v/v PBT and subjected to a second sample fixation in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Genetics 2022Quote: ... adult female intestines were dissected out in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Immunology 2021Quote: ... 48-hour activated T cells from PRMT5fl/fl and T-PRMT5Δ/Δ mice were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min in V-bottom plates (Costar ...
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bioRxiv - Physiology 2021Quote: ... at 370C and washed in Dulbesco Phospate Buffered Saline - DPBSx1 (Biological Industries, Cat# 02-020-1A).Neurons were than briefly fixed (10 minutes) with 4% paraformaldehyde (PFA, Electron microscopy sciences, Cat #BN15710) in DPBSx1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells grown on coverslips were washed in phosphate-buffered saline (PBS) and fixed in freshly prepared PBS supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed by immersion in methanol at -200C for 10 minutes followed by fixation in 4% EM grade paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 10 minutes followed by permeabilization and blocking in PBTA Blocking Solution (PBS 1X ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary tumors were cut in half and either snap-frozen in dry ice or fixed with 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA). At the endpoint ...
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bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
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bioRxiv - Cell Biology 2021Quote: The kidneys containing the grafts were preserved by in vivo intracardiac perfusion in anesthetized mice with heparinized saline buffer (pH 7.4) followed by 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA, USA), and then immersed for 24 hours at 4 °C in the same fixative agent ...
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bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion was done with a prewash with 9.25% sucrose in distilled water and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion TwoTM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... rats were transcardially perfused with an isotonic solution at 9.25% in distilled water as a prewash and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion Two TM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Genomics 2023Quote: ... cells in the 6 well glass bottom plates were fixed by aspirating the medium and replacing it with 4% paraformaldehyde (Electron Microscopy Sciences, 15710-S) in a 1x RNase free PBS solution made from 10x RNase Free PBS (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... Grids were made using a Vitrobot™ Mark IV (FEI) at 4 °C and 100 % humidity with Grade 595 Vitrobot™ filter paper (Electron Microscopy Sciences). All grids were plunge frozen using liquid ethane and stored in liquid nitrogen.
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bioRxiv - Developmental Biology 2023Quote: Gels were fixed by 30 minutes rotation incubation at room temperature in freshly prepared 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA, Cat# 15710S) diluted in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
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bioRxiv - Neuroscience 2022Quote: ... The mammary gland was excised and fixed overnight from either lactating wild-type (C57BL/6) or oxytocin knockout mice in 4% PFA (Electron Microscopy Sciences, Hatfield, PA). Tissue was incubated at 37 °C in CUBIC Reagent 1 A (10 wt% Triton ...
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bioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with ketamine/xylazine and transcardially perfused with PBS followed by PFA (4% in PBS; Electron Microscopy Sciences, cat n°15700). Extracted brains were post-fixed (PFA 4% overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and pancreas were harvested at the experimental endpoint and immediately fixed in 4% paraformaldehyde in 1X PBS (16% solution, Electron Microscopy Sciences, cat# 15711) at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... or any of the four possible distributions at the 4-cell stage (ABa and P2; ABa and EMS; ABp and P2; ABp and EMS), were more likely to harbor sperm-contributed centrioles using the χ2 test as implemented in the library SciPy ([https://scipy.org/citing-scipy/]) ...
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bioRxiv - Neuroscience 2024Quote: ... 240 mg/kg body weight) or on ice (for early postnatal animals) and transcardially perfused with PBS followed by ice-cold 4% paraformaldehyde (EMS #15714, diluted in PBS). Spinal cords were removed and post-fixed for 2 hours (neonatal spinal cords ...
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bioRxiv - Neuroscience 2024Quote: ... animals were anesthetized in 0.02% MS-222 and then immersed in 4% PFA (diluted from 16% PFA in 1xPBS, Cat # 15710, Electron Microscopy Sciences, Fort Washington, PA) using a Pelco Biowave Pro microwave (Model 36700 ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and then perfused transcardially with room temperature (RT) phosphate buffered saline (PBS) with heparin (0.02 mg/mL) and then ice-cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, catalog no: 15714). Brains were dissected and post-fixed in 4% PFA overnight at 4°C and cryopreserved in 30% sucrose in PBS for ∼2 days at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... at the time of brain microdissection the spinal cord was rapidly dissected and immersed in 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA). For assessment of phospho-cJUN and ATF3 expression animals were perfused with 4% PFA ...
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bioRxiv - Neuroscience 2024Quote: ... i.p.) and transcranial perfused with 1 × ice-cold phosphate-buffered saline (PBS) followed by 4% ice-cold paraformaldehyde (PFA, Electron Microscopy Sciences, Pennsylvania, USA) made in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... fixed for 20 min in 4% EM-grade paraformaldehyde (PFA) diluted in phosphate-buffered saline (PBS) from an 8% PFA Aqueous Solution (Electron Microscopy Sciences, 157-8) and blocked for 1 h in PBS plus 0.2% Triton X-100 and 4% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... Brains from adult females were dissected in Phosphate-buffered saline (PBS, Eurobio, ref CS0PBS0108) and fixed for 15 min in 4% paraformaldehyde (Electron Microscopy Sciences, Cat # 15714-S) at room temperature (RT) ...
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bioRxiv - Genetics 2020Quote: ... Then the cells were counted and diluted to 106 cells/ml and fixed with 4% paraformaldehyde aqueous solution (Electron Microscopy Sciences #15710, Formaldehyde methanol-free) for 10 minutes at room temperature (RT) ...
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bioRxiv - Developmental Biology 2021Quote: ... The eye imaginal discs were dissected in cold 1X solution of Phosphate Buffered Saline (PBS) and fixed using 4% p-formaldehyde (Electron Microscopy Sciences, Catalog no. 15710). Fixed tissues were washed using PBS-T [0.2% Triton X-100 (Sigma ...