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Citations for Electron Microscopy Sciences :
2401 - 2450 of 2665 citations for 4' Bromo 2' fluoro 3 2 methoxyphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... mice were perfused transcardially with heparinized saline and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 ul aliquots of the mixture were applied to UltrAu foil R1.2/1.3 gold 300-mesh grids (Electron Microscopy Sciences). Grids were then blotted and vitrified in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Biochemistry 2023Quote: ... transcardial perfusion was performed using 1× phosphate-buffered saline (PBS) and 4% paraformaldehyde (PFA; Electron Microscopy Sciences, the United States). The mouse liver and kidney were dissected and postfixed in 4% PFA for 6 hours at 4°C ...
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bioRxiv - Bioengineering 2022Quote: Microtissues were transferred to wells with 4% PFA in PBS (diluted from 32% PFA solution, Electron Microscopy Sciences 15714-S) for 15 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... lungs were inflated with 1:1 ratio of OCT:Saline and fixed in 4% paraformaldehyde solution (Electron Microscopy Science, PA, USA) before dehydration and paraffin embedding ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 expression was confirmed by immunofluorescence with paraformaldehyde (4% f.c, 15 min, room temperature, Electron Microscopy Sciences, Cat. #15713-S) fixed cells ...
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bioRxiv - Neuroscience 2023Quote: E16.5 and E18.5 embryos were sacrificed by head sectioning and brains were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in Phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... U2OS cells were washed once with PBS and immediately fixed by 4% EM-grade paraformaldehyde (Electron Microscopy Science, Hatfield, PA) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Pin 12.7 mm x 3.1 mm) with a silver filled epoxy (Epoxy Conductive Adhesive, EPO-TEK EE 129-4; EMS, USA) and polymerized at 60° overnight ...
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bioRxiv - Genetics 2023Quote: ... immediately followed by fixation at room temperature for 30 min using a 4% paraformaldehyde solution (Electron Microscopy Sciences, (PN:15714)) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... dissected larva body walls were fixed in Paraformaldehyde (4% from 16% stock of electron microscopy grade; Electron Microscopy Sciences, 15710) for 20 min ...
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bioRxiv - Cell Biology 2024Quote: HeLa and MEF cell lines cultured on glass coverslips were fixed for 10min using 4% paraformaldehyde (Electron Microscopy Sciences #15710), in PBS followed by permeabilization for 20minutes with 0,1% Triton X-100 (Sigma-Aldrich #T8787 ...
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bioRxiv - Molecular Biology 2024Quote: ... heated in Volvic mineral water 5 min at 70°C to extend the tardigrade body and directly fixed with 4% formalin (15686, EMS) in 1X PBS-1% Triton-X100 (PBS-Tx ...
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bioRxiv - Bioengineering 2024Quote: Cells were cultured on NP hydrogels for 48 hours before fixing with 4% (w/v) paraformaldehyde (Electron Microscopy Sciences, 15710) for 20 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 3 μl of the GII.4 RdRp (diluted 10 times) was adsorbed onto a glow-discharged grid carbon-coated grid (CF200-CU; EMS) for 60 s ...
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bioRxiv - Immunology 2024Quote: ... transcardial perfusion with ice-cold PBS was followed by perfusion with 10-20 mL of buffered 4% paraformaldehyde (PFA, EMS).
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized by IP injection of Avertin and transcardially perfused with PBS followed by 4% paraformaldehyde (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... For all experimental approaches cells were washed with PBS once and fixated in 4% PFA (Electron Microscopy Sciences, #15714-S) in PBS (Thermo Fischer ...
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bioRxiv - Immunology 2024Quote: Neutrophils migrating under the agarose for 1 h in 8 well chamber dishes were fixed using 4% paraformaldehyde (PFA, EMS #) in PHEM buffer for 20 min at 37 °C followed by agarose removal from the wells ...
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bioRxiv - Neuroscience 2024Quote: ... animals were transcardially perfused with 20 mL of ∼37°C PBS 0.1M followed by 20 mL of ice-cold 4% paraformaldehyde (EMS, 15714S) in PBS 0.1M ...
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bioRxiv - Bioengineering 2024Quote: ... mice were perfused transcardially with heparinized saline (10 units mL−1 of heparin) and 4% paraformaldehyde (PFA) that was freshly prepared from 32% PFA aqueous solution (Cat# 15 714, EMS), using a peristaltic pump at a rate of 7 mL min−1 ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: Flow chambers were created using tape placed ~3 mm apart on a glass slide to adhere coverslips coated with 0.1% collodion in amyl acetate (Electron Microscopy Sciences, Hatfield, PA). For standard gliding assays with β-cardiac myosin eGFP ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Cell Biology 2023Quote: ... astrocytes loaded with either unlabeled or AF647 NHS ester-labeled tau fibrils (0N4R PFF, described earlier in methods) were fixed with 3% (vol/vol) paraformaldehyde (Electron Microscopy Sciences, 15714) and 0.1% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... Ileum pieces were rinsed 3 times for 5 minutes in PB buffer and then postfixed with a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA, US) with 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... anesthetized larvae were moved to a rectangular plate (4.5 cm x 3 cm x 1 cm) coated with Sylgard (Electron Microscopy Sciences, Hatfield, PA). After removing excess water ...
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bioRxiv - Cell Biology 2022Quote: ... 3) three double-sided 9 x 0.12 mm round well electron microscopy grade double sided adhesive spacers (Electron Microscopy Sciences, Cat# 70327-8S), 4 ...
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bioRxiv - Evolutionary Biology 2024Quote: Negative staining grids were prepared using a standard protocol,86 pipetting 3-8 µL samples on glow discharged ultrathin carbon film supported copper grids (EMS CF400-Cu-UL), followed by staining with 2% (w/v ...
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bioRxiv - Physiology 2024Quote: ... Hearts were perfused for 3 min with PBS at 37°C to wash out the blood followed by a fixative solution of Glutaraldehyde 3% in 0.1M Cacodylate buffer pH7.4 (Electron Microscopy Sciences-cat#16538-15) for less than 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... the samples were rinsed 3 times for 5 min each in PB buffer and postfixed with a fresh mixture of 1% osmium tetroxide (EMS, Hatfield, PA, US) and 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed with 100 µl of warm PBS and fixed with 4% methanol-free paraformaldehyde (PFA) (Electron Microscopy Sciences) for 15 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... image positions were recorded and slides were washed in 1xPBS/0.2% Tween 20 to remove the mounting medium and re-fixed in 4% paraformaldehyde (Electron Microscopy Sciences) prior to DNA FISH experiment ...
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bioRxiv - Cell Biology 2020Quote: ... The slices were then transferred to capped glass vials and postfixed with 1% osmium tetroxide diluted in PBS from a 4% aqueous solution (EMS 19170) for 30 minutes on a rotator ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized with isofluorane (Forane), and then perfused with 4% paraformaldehyde (PFA, Electron Microscopy Services) and 0.005% glutaraldehyde (GA, Electron Microscopy Sciences) in 175 mM sodium phosphate buffer (PB ...
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bioRxiv - Cell Biology 2021Quote: ... Post-fixation was performed for 1 hour at 4 °C using 1 % osmium tetroxide and 0.8 % potassium ferracyanide (Electron Microscopy Sciences) in 0.1 mol/l cacodylate buffer ...
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bioRxiv - Microbiology 2020Quote: ... virus- or mock-infected cells were detached using 0.06% trypsin-EDTA in phosphate-buffered saline (PBS) and fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in PBS for 20 minutes at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... neurons were rinsed with sterile Hank’s Balanced Salt Solution and fixed for 10min with pre-warmed 4% PFA (Electron Microscopy Sciences) in Phosphate Buffered Saline (PBS) ...
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bioRxiv - Molecular Biology 2022Quote: ... Media was aspirated and cells were washed with PBS at RT once before being incubated with 4% PFA (Electron Microscopy Sciences) made up in PBS with the addition of 4% sucrose at RT for 15 mins ...
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bioRxiv - Cell Biology 2022Quote: ... high parasitemia culture was pelleted by centrifugation (1.5 g, 3min, RT) and resuspended in freshly prepared PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) and 0.0075% gluteraldehyde (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Mice were anesthetized and transcardially perfused with 0.1M phosphate buffer (PBS) followed by 4% EM-grade paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were dissected and post-fixed overnight in 4% PFA ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were fixed for 15 min at RT with 4% electron microscopy grade paraformaldehyde (PFA; Electron Microscopy Sciences, cat. no. 15710) diluted in PIPES–EGTA– magnesium (PEM ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was removed and the neurons were fixed with 4% electron-microscopy-grade PFA (Electron Microscopy Sciences, cat. no. 15710) diluted in a cytoskeleton-protective buffer (PEM ...
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bioRxiv - Cell Biology 2020Quote: ... a sucrose gradient was created using 2.3 ml of a 20 % sucrose solution containing 15 mM HEPES [pH 7.4] and 4 % paraformaldehyde aqueous solution (EM Grade) (Electron Microscopy Sciences) and 2 ml of a 10% sucrose solution containing 15 mM HEPES [pH 7.4] ...
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bioRxiv - Microbiology 2021Quote: ... Monolayers were fixed and plaques visualized with a solution of 0.4% crystal violet by weight in 80% methanol (MilliporeSigma) and 4% PFA (Electron Microscopy Sciences) for 20 min at room-temperature (RT).
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bioRxiv - Biochemistry 2021Quote: Samples (4 μL) were applied to glow-discharged continuous carbon film supported copper grids (CF300-CU-50 grids, Electron Microscopy Sciences). After 1 min incubation ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2021Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Immunology 2020Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...