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Citations for Electron Microscopy Sciences :
2551 - 2600 of 2685 citations for 8 BROMO 2 3 4 5 TETRAHYDRO 1H PYRIDO 4 3 B INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
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bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde in 0.1M Sodium Cacodylate buffer (cat. no. 15960-01 Electron Microscopy Sciences) for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat ...
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bioRxiv - Biophysics 2024Quote: ... 2.5 µl of sample was applied to 300 mesh Quantifoil R 2/1 copper grids (Electron Microscopy Sciences, Hatfield PA) that had been previously glow discharged (15 mA ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were then mounted with DAPI (diamidino-2-phenylindole)-free Fluoro-Gel on glass slides (Electron Microscopy Sciences, USA). Cells were scanned using a 63x oil immersion objective mounted on a confocal microscope (LSM 710 Meta ...
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bioRxiv - Microbiology 2023Quote: Immunofluorescence assay was performed on PHA-E-immobilised parasites by fixation with 2% paraformaldehyde and 0.008% glutaraldehyde (Electron Microscopy Sciences) and permeabilisation with 0.1% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed with glutaraldehyde 2.5% in 0.1 M sodium cacodylate buffer supplemented with 2 mM of calcium chloride (Electron Microscopy Sciences), post-fixed with 1% OsO4 and 1.5% potassium ferrocyanide in sodium cacodylate buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the cells on nanostructures were washed with PBS and fixed in 0.1 M sodium cacodylate buffer (pH 7.2) supplemented with 2% glutaraldehyde (Electron Microscopy Sciences) and 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed in water and stained for 1 h at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), and then washed again in water and dehydrated in a graded series of ethanol ...
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bioRxiv - Plant Biology 2023Quote: ... and the resulting cell pellets were fixed using a mixture of 2% [v/v] paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) and 2% [v/v] glutaraldehyde (Agar Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... They were subsequently transcardially perfused with 15 ml of 0.1M sodium phosphate buffer pH 7.4 and then 60 ml of 2% paraformaldehyde (15710-S, Electron Microscopy Sciences) 1% glutaraldehyde (G7526 ...
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bioRxiv - Microbiology 2023Quote: Genomic libraries were constructed and sequenced by MiSeq using paired-end sequencing (2 × 300 bp) for 17 strains generated by EMS mutagenesis and one strain ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
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bioRxiv - Genetics 2023Quote: ... the supernatant was discarded and the pellet resuspended in primary fixating buffer (2% glutaraldehyde/0.1 M sodium cacodylate buffer, pH 7.4, Electron Microscopy Sciences) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed in 0.1 M cacodylate buffer (pH 7.4) with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were washed in water and stained for 1 hour at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), washed again in water and dehydrated in a graded series of ethanol ...
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bioRxiv - Biochemistry 2024Quote: Prom1 and Ttyh1 EVs were vitrified on 300-mesh gold Quantifoil R 1.2/1.3 + 2 nm Carbon grids (Electron Microscopy Sciences). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... en bloc staining protocol (Kislinger et al., 2020a) (Kislinger et al., 2023) including post-fixation in 2% osmium tetroxide (EMS), 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Pathology 2024Quote: ... the sections were heated to 650C for 1-2 hours to deparaffinize and treated with Histoclear II (Electron Microscopy Sciences) twice for 15 minutes each ...
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bioRxiv - Genetics 2024Quote: ... The coverslip was mounted onto a concave microscope slide to reduce media evaporation (2-Well Concavity Slide, Electron Microscopy Sciences). Images were acquired using an AX70 Olympus epifluorescence microscope across 11 z-planes with 0.5 µm of interplanar distance ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were rinsed three times with PBST for 5 min on ice and then embedded and frozen in Tissue Freezing Medium (Electron Microscopy Sciences, PA). Samples were sectioned (8μm ...
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bioRxiv - Biochemistry 2020Quote: End-point samples from wt α-syn aggregation assays (5 μL) were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 seconds using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg of the sample was placed onto a glow discharged 200-mesh formvar/carbon copper grid (Electron Microscopy Sciences, Hatfield Township, PA) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed three times for 5 min in PBS 1X and mounted using Fluoromont G fluorescence mounting medium (EMS, No. 17984-25). For all immunofluorescence analysis ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Molecular Biology 2021Quote: BF PolIE RNAi cells were grown with or without doxycycline for 24 hours then incubated with 2 mM of ethylmethanesulfonate (EMS; Sigma) or DMSO (vehicle ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3 μl of the particle dispersion was applied on 400-mesh C-Flat CF-2/1-4C copper grids (Protochips, USA) negatively glow discharged with a Plasma Cleaner (EMS, USA) at 50 mA for 45 s ...
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bioRxiv - Zoology 2021Quote: Sets of oocytes including some number of visibly infected individuals were fixed in 2% glutaraldehyde in 0.2 M sodium cacodylate buffer (SCB, Electron Microscopy Sciences) for one hour and post-fixed in 2% osmium tetroxide in SCB for one hour (Bozzola and Russell ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% uranyl acetate and 2% H2O followed by en bloc Osmium-Thiocarbohydrazide-Osmium staining75,76 and embedding in Durcupan ACM (Electron Microscopy Sciences). Serial block-face imaging was conducted in a Zeiss Sigma 3View system with variable pressure with 30 nm x 70 nm resolution at 5.5 kV ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on bone slices for 3 days were unroofed as described above and fixed for 10 min in a 0.2M sodium cacodylate buffer (pH 7.4) containing 2% paraformaldehyde (Electron Microscopy Sciences 15710) and 2.5% glutaraldehyde (Electron Microscopy Sciences 16220) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 3.2 μL of RNCs were incubated on plasma-cleaned 300-mesh holey carbon grids (C-flat R2/2, Electron Microscopy Science) for 1 min ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μl of diluted sample was deposited onto a glow-discharged (Edwards S150B, 30sec, 30-50 mA, 1,2kV, 10-2 mbar) continuous carbon grid (CF400-CU-UL, Electron Microscopy Sciences). After one minute at RT ...
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bioRxiv - Biophysics 2022Quote: ... then cells were allowed to adhere for 15 min in media prior to chemical fixation in 2% PFA and 0.2% glutaraldehyde (Electron Microscopy Sciences). FActin was stained by permeabilizing cells with 0.1% Triton-X-100 prior to incubation with 3.3 μM phalloidin-AlexaFluor647 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: Glass vials were prepared prior to the experiments with a 2-mm layer of Sylgard 184 (Electron Microscopy Sciences, Hatfield, PA) at the bottom ...
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bioRxiv - Neuroscience 2020Quote: ... 20 mM NaCl and incubated with secondary 15 nm gold-tagged goat-anti-rabbit IgG F(ab’)2 (Electron Microscopy Sciences) diluted 1:10 in 20 mM Tris pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were taken prior to the experiment (‘Pre’) and at each time point by diluting 50 μl of cells 1:1 with 2% glutaraldehyde (EMS, 16220) and incubating at 4°C to allow cells to sediment ...
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bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: 63 day old hMO specimens were immersion-fixed in a solution of 2 % PFA and 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences, Hatfield, PA) for 3 h ...
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bioRxiv - Bioengineering 2020Quote: ... bone pieces were washed with double distilled water for 2 minutes and embedded in Epon epoxy resin dissolved in acetone (Embed 812, EMS, USA). The Epon embedding was carried out in 5 steps ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... atala eye was immersed for prefixation in 2.5% Glutaraldehyde/2% paraformaldehyde in 0.1M Sodium Cacodylate buffer (pH 7.4) (Electron Microscopy Sciences, PA, USA) for 2h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Copper grids containing thin sections were post-stained for 20 min in 2% aqueous uranyl acetate (Electron Microscopy Sciences; Cat #22400) and for 5 min in Reynolds lead citrate (Reynolds ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were transfer into slicksealTM microcentrifuge tube (National Scientific, CA170S-BP) and fixed with 2% paraformaldehyde (Electron Microscopy Science, Nm15710) in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 ul chromatin samples with a concentration of about 0.2 mg/ml DNA were applied to Quantifoil R2/2 200 mesh copper grids (EMS Q250-CR2). Vitrification was conducted by plunging into liquid ethane using our FEI Vitrobot Mk IV Grid Plunging System at 100% humidity ...
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bioRxiv - Cancer Biology 2022Quote: ... sample coverslips were washed 2 times in staining buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
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bioRxiv - Neuroscience 2022Quote: ... P21 and P28) were deeply anesthetized and perfused with 0.9% saline followed by 2% paraformaldehyde (PFA) and 2.5% glutaraldehyde (EMS, Hatfield, PA, USA) in 0.1 M phosphate buffer (PB) ...
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bioRxiv - Cancer Biology 2024Quote: ... sample slides were washed 2 times in Staining Buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) storage buffer ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Molecular Biology 2023Quote: HEK-293 cells (60%-70% of confluency) were fixed by 2.5% glutaraldehyde and 2% paraformaldehyde (both from Electron Microscopy Science, Inc.) solution for 1 h at 4°C ...