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Citations for Electron Microscopy Sciences :
2451 - 2500 of 2685 citations for 8 BROMO 2 3 4 5 TETRAHYDRO 1H PYRIDO 4 3 B INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The pellet was resuspended in buffer (0.2 M HEPES, pH 7.4, 5 mM CaCl2) and bacteria were fixed by addition of glutaraldehyde (Electron Microscopy Sciences) in buffer to a final concentration of 2.5% for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The sample was then mounted on a SEM sample holder with double-sided carbon tape and sputter-coated with 5 nm of Pt/Pd (EMS 150 T S Metal Sputter Coater ...
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bioRxiv - Biophysics 2021Quote: ... after which 5-μL were deposited in a glow-discharged Quantifoil grid (200-mesh copper R2/1, Electron Microscopy Sciences), followed by manual blotting and plunge freezing in a 50/50 ethane propane mix (Airgas ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: For collagen staining deparaffinized and re-hydrated slides were stained for 5 min in Van Gieson solution (EMS, 26374-06) followed by dehydration by 2 changes in 100% ethanol and cleared by 2 changes of xylenes ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μl of the mixed solution was dropped onto the 200 mesh formvar/carbon copper grid (Electron Microscopy Sciences, USA), allowing it to almost dry for 20 minutes ...
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bioRxiv - Microbiology 2024Quote: ... A 5 μl aliquot of the sample was deposited onto a 300 mesh copper grid coated with carbon film (EMS). After allowing the sample to interact with the grid for 60 seconds ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of the endosomal suspension was spotted on a mica sheet (Muscovite Mica-V1, Electron Microscopy Sciences, Hatfield, PA) and dried for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Bioengineering 2023Quote: 5 μL of D-GQDs/exo loaded on the Poly-L-Lysine coated slides (63410; Electron Microscopy Sciences, PA, USA) and was fully covered with 18 mm × 18 mm square cover glasses (2845-18 ...
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bioRxiv - Cell Biology 2024Quote: ... The stock solution was first diluted in DMSO to make a 5 mM solution and then further diluted in perfusion media (EMS) to give the final concentration of 1 μM for drug treatment and 0.02% DMSO for the vehicle control ...
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bioRxiv - Cell Biology 2020Quote: ... Grids with the collected monolayers facing up were stained at once with 2% uranyl formate (Electron Microscopy Sciences) in water by depositing 4 μL on the grid and simultaneously blotting the excess solution ...
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bioRxiv - Biophysics 2020Quote: ... and permeabilized for 2 min with 0.3 % glutaraldehyde (GA) + 0.25 % Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37° C ...
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bioRxiv - Neuroscience 2021Quote: ... followed by fixation with 2% PFA (w/v; prepared from a 20% stock solution, Electron Microscopy Sciences: 15713) in cell culture medium for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... following which they were placed in a mixture (500 μL) of 2% OsO4 (Electron Microscopy Sciences, cat#19150) and 0.1 M sodium cacodylate buffer for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were then postfixed for 1 h on ice with 2 mL of 1% osmium tetroxide (EMS #RT19134) and 1% potassium ferrocyanide (Sigma-Aldrich #P3289 ...
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bioRxiv - Biophysics 2020Quote: ... and permeabilized for 2 min with 0.3 % glutaraldehyde (GA) + 0.25 % Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37° C ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µL of particle solution (20 mg/mL) was applied on a CF400-CU grid (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were fixed in 2% PFA/2.5% glutaraldehyde/0.1 M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Lateral ventricle walls were fixed for 18 h at 4 °C othen subdissected into anterior ...
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bioRxiv - Neuroscience 2020Quote: ... unbuffered OsO4 aqueous solution for 2 hours and lead aspartate solution (0.03 M, pH 5.0 adjusted by KOH, EMS) at 50 °C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were intracardially perfused with NaCl 0.9% followed by PFA 2% (Electron Microscopy Sciences 15713, diluted in PBS). Brains were post-fixed in PFA 2% and cryoprotected in 20% sucrose overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were stained by a 2 minute bath in 0.1% cresyl violet acetate (Electron Microscopy Sciences #26089-01), followed by a 2 minute rinse in distilled water ...
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bioRxiv - Microbiology 2020Quote: ... and stained with 2% uranyl formate (pH 5.5– 6.0) on thin carbon-layered 400 mesh copper grids (EMS)46 ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with pentobarbital and perfused with 2% paraformaldehyde (PFA, Paraformaldehyde Granular, Electron Microscopy Sciences, Hatfield, PA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... (2) fixed with 1.5% paraformaldehyde and 1.5% glutaraldehyde in 0.1M sodium cacodylate (Electron Microscopy Sciences, Hatfield, PA, USA), pH 7.3 for 1 h at room temperature followed by 3 washes with 0.1M sodium cacodylate ...
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bioRxiv - Biophysics 2024Quote: 3 μL of purified MscS between 0.1 and 0.7 mg/mL was applied to a glow discharged Quantifoil R 1.2/1.3 + 2 nm C 400 Cu mesh grid (EMS), a holey grid with a thin 2 nm continuous carbon film ...
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bioRxiv - Physiology 2023Quote: ... Ovaries used for MIMS were cut in half and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) and embedded in LR white (EMS) ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed in freshly diluted 2% paraformaldehyde (PFA) in PBS (16% PFA purchased from EMS Cat# 15710) for 20 minutes at room temperature in the dark ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of the diluted DNA solution was dropped onto a carbon film coated TEM grid (Electron Microscopy Sciences, PA, USA), and incubated at room temperature for 1.5 min ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of particles in suspension were mounted on a copper grid of Formvar 300 mesh/carbon (Electron Microscopy Science, PA). Samples were dried at room temperature for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... DE) was diluted to 5 μg/mL in assay buffer: 1X PBS with 0.1% acetylated BSA (Electron Microscopy Sciences, Hatfield, PA) in a 96-well plate and loaded onto streptavidin biosensors (FortéBio ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at the Pole Facultaire de Microscopie Electronique at the University of Geneva as described 5 except uranyl acetate staining was replaced with Reynold’s (lead citrate, Electron Microscopy Sciences), and 20 nm sections were used ...
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bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
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bioRxiv - Microbiology 2019Quote: ... for 5 min and the coverslips were mounted onto glass slides with CitiFluor AF1 antifading (Electron Microscopy Sciences, Emsdiasum, Hatfield Penn). Images were acquired with an inverted 3I spinning disk confocal microscope (Zeiss Observer Z.1 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Immunology 2020Quote: ... ~ 5 μL of the sonicated samples were drop cast onto 300-mesh formvar carbon-coated copper grids (Electron Microscopy Sciences, UK) and negatively stained with UranyLess (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on studs using silver paint and coated with 5 nm of Palladium (sputter coater EMS150TS; Electron Microscopy Sciences). Samples were imaged at 5 kV on a FEI Magellan 400 XHR Field Emission Scanning Electron Microscope at the Stanford Nano Shared Facilities.
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bioRxiv - Cancer Biology 2024Quote: ... and pellets were resuspended in a 5 ml volume of fixative master mix of Paraformaldehyde-Glutaraldehyde Solution Karnovsky’s Fixative (Electron Microscopy Sciences). Fixative solution was prepared with 16% paraformaldehyde solution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 µL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... was infused into the well of the chamber and covered with a 5-mm diameter glass coverslip (7229605, Electron Microscopy Sciences). The skin was sealed with VetbondTM Adhesive ...
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bioRxiv - Biophysics 2023Quote: ... 5 µls were applied to a glow discharged 1.2/1.3 CF-1.2/1.3-3Au-50 (Electron Microscopy Sciences, part # AUFT313-50) cryo-EM grid and allowed to absorb for 30 sec ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS with 0.2% Triton X-100 and mounted onto slides with DAPI Fluoromount (#17984-24, Electron Microscopy Sciences) before visualization with an EVOS imaging system (Thermo Fisher Sci).
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg of EVs was placed onto glow discharged 200-mesh formvar/carbon copper grids (Electron Microscopy Sciences, Hatfield Township, PA). The EVs were washed two times with water and then fixed in 2.5% glutaraldehyde ...
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bioRxiv - Microbiology 2023Quote: ... Slides were subsequently washed three more times for 5 minutes by immersion in 0.1% Tween-20 in PBS and mounted in EMS Shield Mount with DABCO (Electron Microscopy Sciences). Imaging was performed using a Carl Zeiss LSM710 confocal microscope at the microscopy imaging center (MIC ...
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bioRxiv - Plant Biology 2024Quote: Aliquots (5 µL) of resuspended P40 pellets were spotted onto Formvar and carbon-coated copper electron microscopy grids (Electron Microscopy Sciences) that were glow-discharged at 15mA for 60 seconds prior to application of samples ...
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bioRxiv - Neuroscience 2024Quote: ... and the hole in the bone was covered with a round cover glass (d = 5 mm; Electron Microscopy Sciences, 72296–05). A helicopter-shaped head plate (model 1 ...