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Citations for Electron Microscopy Sciences :
201 - 250 of 953 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Neuroscience 2023Quote: ... At −50 °C samples were washed four times in ethanol for 30 minutes each before gradual infiltration with HM20 resin (Electron Microscopy Sciences). Polymerization of the resin was done with a UV light source and was carried out at −50 °C for 48 hours followed by post-polymerization at room temperature for 2 days.
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bioRxiv - Immunology 2023Quote: ... A 3-µl drop of the mixture was applied to a plasma-cleaned C-flat 2/2 holey carbon grid (Electron Microscopy Sciences), manually blotted and vitrified in liquid ethane with a homemade plunger.
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4 buffer for P116 without the N-terminal domain (246–818)) was applied to a 45 s glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed with pre-warmed (37°C) PBS and permeabilized for 2 minutes using 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... mice were perfused with phosphate-buffered saline (PBS, warmed up to 37 °C) with heparin solution (20 units / mL) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science) in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... nrpe1-1 (EMS), and drm1-2 (SALK_031705 ...
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bioRxiv - Cell Biology 2023Quote: ... containing 0.1% tanic acid (EMS),1% glutaraldehyde (EMS) in anhydrous acetone (EMS ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.3-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2021Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP expression in OB was detected using a dual fluorescent protein flashlight (Electron microscopy sciences, Hatfield, PA, USA) prior to surgery ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of protein sample was applied to freshly-ionized carbon-coated copper mesh grids (Electron Microscopy Science). After 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of protein was loaded onto a glow-discharged CF200-CU carbon grid (Electron Microscopy Sciences, U.K.). After 5 min of incubation ...
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bioRxiv - Immunology 2023Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 s using a PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Cell Biology 2020Quote: ... atgl-1(xd310) and atgl-1(xd314) were generated by EMS. xdIs182 (Prab-3::Cre/Podr-1::RFP ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then embedded in 1:1 Embed-812 (EMS 14120):acetonitrile ...
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bioRxiv - Microbiology 2023Quote: ... immediately stained with 1% uranyl acetate (Electron Microscopy Sciences, 22400-1) for 30 seconds ...
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bioRxiv - Neuroscience 2020Quote: ... rats were deeply anesthetized with sodium pentobarbital (180 mg/kg; Fatal-Plus C IIN, Vortech Pharmaceuticals, Dearborn, MI) and perfused with paraformaldehyde (#15710, Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μL of the sample was repeatedly applied and manually blotted three times onto 300 mesh R1.2/R1.3 C-flat Holey carbon grids (Electron Microscopy Sciences, Hatfield, PA, USA) using the multiple blotting technique prior to the Vitrobot step to increase the concentration of pili (34) ...
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bioRxiv - Biochemistry 2022Quote: ... sarkosyl and 4 μl of the suspension was applied directly to a glow-discharged C-flat™ Holey Carbon CF-2/2-4C Cu 400 mesh cryo-EM grid (Electron Microscopy Sciences) or Quantifoil R2/2 Cu 300 mesh grids in the chamber of the Leica GP2 plunging robot ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM samples of both EVs and LUVs were prepared on either lacey carbon or C – flat EM grids (Electron Microscopy Sciences, USA), on which 10 nm Protein A colloidal gold particles (Au – NP ...
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bioRxiv - Neuroscience 2022Quote: ... The 40 μm cryosections were cut at −18°C and placed over glass slides treated with tissue capture pen (Electron Microscopy Sciences 71314) and stored at −80°C ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were dehydrated in graded acetone series and embedded for 72 hours at 60°C in Embed-812 resin (EMS, Hatfield, PA, USA). Ultrathin sections were collected on copper grids ...
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bioRxiv - Biochemistry 2021Quote: ... sarkosyl and 4 μl of the suspension was applied directly to a glow-discharged C-flat™ Holey Carbon CF-2/2-4C Cu 400 mesh cryo-EM grid (Electron Microscopy Sciences) in the chamber of the Leica GP2 plunging robot ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Plant Biology 2022Quote: ... from −30 °C to 0° in 6 h) This was followed by washing in acetone and then infiltration in Spurr resin (EMS, Hatfield, PA, US) at graded concentration and temperature (30% for 10 h from 0 °C to 20°C ...
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bioRxiv - Cell Biology 2023Quote: ... The filters were then removed from the culture dishes and incubated overnight in 4% paraformaldehyde solution at 4 °C (Electron Microscopy Sciences #15713). In preparation for paraffin embedding and sectioning ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Biophysics 2023Quote: A volume of 3.5 μl of purified channel protein was applied onto glow-discharged copper holey carbon grids R1.2/1.3 (Q350CR1.3, Electron Microscopy Sciences), which was cleaned for 1 minute in an H2/O2 plasma using a Gatan Solarus 950 (Gatan) ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Neuroscience 2021Quote: ... and then post-fixed for 1 hr in 1% osmium tetroxide (EMS) in 0.1 M sodium cacodylate ...
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bioRxiv - Biophysics 2020Quote: Solutions of extruded vesicles were mixed with 6 nm colloidal gold fiducial markers (Aurion, Wageningen, Netherlands) and applied to glow-discharged C-flat holey carbon grids (Electron Microscopy Science, Hatfield, PA) or QUANTIFOIL R 2/2 holey carbon grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2021Quote: ... Cochleae from C57BL/6J wild-type mice at P6 and P14 were fixed overnight at 4°C in 4% PFA (Electron Microscopy Sciences, Hatfield, PA) in 1x PBS ...
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bioRxiv - Biophysics 2019Quote: ... and then fixed for 15 minutes at room temperature (RT) with pre-warmed (at 37 °C) 3% paraformaldehyde (PFA, 15710, Electron Microscopy Sciences, Hatfield, PA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Biophysics 2023Quote: ... Grids were made using a Vitrobot™ Mark IV (FEI) at 4 °C and 100 % humidity with Grade 595 Vitrobot™ filter paper (Electron Microscopy Sciences). All grids were plunge frozen using liquid ethane and stored in liquid nitrogen.
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bioRxiv - Biophysics 2024Quote: ... cells were imaged under physiological conditions (5% CO2 and 37°C) via a stage top incubator (Tokai Hit) with type 37 immersion oil (Electron Microscopy Sciences, #16914-01). Fixed cells were imaged at room temperature (22°C ...
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bioRxiv - Physiology 2024Quote: ... The brain was removed from the skull and a ∼6 mm thick section collected from the optic chiasm to the mamillary bodies using a rat coronal brain matrix (Electron Microscopy Sciences, 69083-C). The hypothalamus was isolated by removing brain matter above the top of the third ventricle and lateral to the optic tract ...
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bioRxiv - Biophysics 2020Quote: ... 1% Tannic acid (EMS) and 1 % Uranyl acetate (EMS) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% osmium tetroxide (EMS) in 0.1M cacodylate buffer was used as a secondary fixative with incubation for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% GA (EMS) in 0.1M Phosphate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 1% uranyl acetate (EMS), and 0.66% aspartic acid buffered lead (II ...