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Citations for Electron Microscopy Sciences :
2201 - 2250 of 2293 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: Cryo-EM samples of both EVs and LUVs were prepared on either lacey carbon or C – flat EM grids (Electron Microscopy Sciences, USA), on which 10 nm Protein A colloidal gold particles (Au – NP ...
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bioRxiv - Neuroscience 2022Quote: ... The 40 μm cryosections were cut at −18°C and placed over glass slides treated with tissue capture pen (Electron Microscopy Sciences 71314) and stored at −80°C ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were dehydrated in graded acetone series and embedded for 72 hours at 60°C in Embed-812 resin (EMS, Hatfield, PA, USA). Ultrathin sections were collected on copper grids ...
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bioRxiv - Biochemistry 2021Quote: ... sarkosyl and 4 μl of the suspension was applied directly to a glow-discharged C-flat™ Holey Carbon CF-2/2-4C Cu 400 mesh cryo-EM grid (Electron Microscopy Sciences) in the chamber of the Leica GP2 plunging robot ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Immunology 2024Quote: ... 3 µL complex was mixed with 1 µL 20 µM lauryl maltose neopentyl glycol (LMNG) and immediately applied to glow discharged C-Flat™-2/1-3Cu-T50 (Electron Microscopy Sciences) holey grids at 4 °C and 100 % humidity inside a Vitrobot Mark IV ...
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bioRxiv - Biophysics 2024Quote: Cryo-EM samples of concentrated peptide solutions were prepared on either 300 mesh 2/2 Quantifoil or C-flat EM grids (Electron Microscopy Sciences, USA), on which 10 nm protein A-conjugated colloidal gold particles (Au–NP ...
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bioRxiv - Neuroscience 2021Quote: ... and washed again twice for 5 minutes each in PBS before coverslipping with Fluoro-Gel (#17985, Electron Microscopy Sciences). Primary antibodies used include ...
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bioRxiv - Synthetic Biology 2021Quote: ... samples (5 μL) were loaded onto a glow discharged Formvar/carbon-coated copper grid (400 mesh, Electron Microscopy Sciences) and stained with 2% uranyl formate ...
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bioRxiv - Biophysics 2023Quote: ... Grids were picked up from the glass bottom MatTek dish using 5/15 Style Dumont Tweezers (Electron Microscopy Sciences), transferred to their respective tweezers and placed into the EM GP chamber set at 25°C and 99% humidity or the CP3 chamber ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently mounted on glass slides in Citifluor Mountant Solution (Electron Microscopy Sciences, cat no. AF100-5 & 17977-150). Slides were imaged on a W1-Tokogawa spinning disk confocal microscope using a Nikon 100x ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Brain sections (5 μm thick) were prepared and stained with Luxol Fast Blue (Electron Microscopy Sciences, Hatfield, PA, USA). Slides were kept in LFB solution and incubated at 58°C for 16 hours.
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bioRxiv - Bioengineering 2024Quote: ... Micelle solution (5 µM) was added to a carbon support TEM grid (200 mesh, Electron Microscopy Sciences, Hatfield, PA). After 5 minutes of incubation ...
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bioRxiv - Biochemistry 2022Quote: ... for four hours at room temperature (ref: “C. elegans: a practical approach”, chapter “Conventional Genetics” by Jonathan Hodgkin, “Protocol 1-EMS Mutagenesis”, pages 252-253) and selected for increased survival NGM-OP50-1 plates with on 5 mM −7.5 mM DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
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bioRxiv - Immunology 2021Quote: For negative staining 5 µl of NLRP3 sample was placed on a copper grid (Electron Microscopy Sciences, cat. no: FCF400CU50), incubated for 1 min ...
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bioRxiv - Bioengineering 2021Quote: ... An aliquot (5 μl) of each sample was placed onto a CF300-Ni mesh Nickel grid (Electron Microscopy Sciences, EMS). The sample grid was deposited for 1 minute using 2% uranyl acetate solution ...
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bioRxiv - Bioengineering 2021Quote: ... An aliquot (5 μl) of each sample was placed onto a CF300-Ni mesh Nickel grid (Electron Microscopy Sciences, EMS). The sample grid was deposited for 1 minute using 2% uranyl acetate solution ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The sample was then mounted on a SEM sample holder with double-sided carbon tape and sputter-coated with 5 nm of Pt/Pd (EMS 150 T S Metal Sputter Coater ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
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bioRxiv - Biophysics 2021Quote: ... after which 5-μL were deposited in a glow-discharged Quantifoil grid (200-mesh copper R2/1, Electron Microscopy Sciences), followed by manual blotting and plunge freezing in a 50/50 ethane propane mix (Airgas ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: For collagen staining deparaffinized and re-hydrated slides were stained for 5 min in Van Gieson solution (EMS, 26374-06) followed by dehydration by 2 changes in 100% ethanol and cleared by 2 changes of xylenes ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of the endosomal suspension was spotted on a mica sheet (Muscovite Mica-V1, Electron Microscopy Sciences, Hatfield, PA) and dried for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μl of the mixed solution was dropped onto the 200 mesh formvar/carbon copper grid (Electron Microscopy Sciences, USA), allowing it to almost dry for 20 minutes ...
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bioRxiv - Microbiology 2024Quote: ... A 5 μl aliquot of the sample was deposited onto a 300 mesh copper grid coated with carbon film (EMS). After allowing the sample to interact with the grid for 60 seconds ...
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bioRxiv - Bioengineering 2023Quote: 5 μL of D-GQDs/exo loaded on the Poly-L-Lysine coated slides (63410; Electron Microscopy Sciences, PA, USA) and was fully covered with 18 mm × 18 mm square cover glasses (2845-18 ...
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bioRxiv - Cell Biology 2024Quote: ... The stock solution was first diluted in DMSO to make a 5 mM solution and then further diluted in perfusion media (EMS) to give the final concentration of 1 μM for drug treatment and 0.02% DMSO for the vehicle control ...
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bioRxiv - Biochemistry 2024Quote: ... AFM samples were prepared by adsorbing 10 μl of 5 times diluted sample onto mica (Muscovite mica, V 1 quality, EMS) for 45 minutes ...
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bioRxiv - Physiology 2020Quote: ... at −20°C with a thickness of 10um through the mid-belly and mounted on SuperFrost glass slides (Electron Microscopy Sciences, catalog no. 71882-01). For analysis of fiber cross-sectional area (CSA) ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of the diluted DNA solution was dropped onto a carbon film coated TEM grid (Electron Microscopy Sciences, PA, USA), and incubated at room temperature for 1.5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... DE) was diluted to 5 μg/mL in assay buffer: 1X PBS with 0.1% acetylated BSA (Electron Microscopy Sciences, Hatfield, PA) in a 96-well plate and loaded onto streptavidin biosensors (FortéBio ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at the Pole Facultaire de Microscopie Electronique at the University of Geneva as described 5 except uranyl acetate staining was replaced with Reynold’s (lead citrate, Electron Microscopy Sciences), and 20 nm sections were used ...
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bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... in the presence of 1 mM ATPγS for 5 min prior to Vitrobot freezing on 300 mesh R 2/2 Quantifoil copper grids (Electron Microscopy Sciences). All three datasets were collected on a FEI Titan Krios TEM with a GATAN post column energy filter (20 eV zero-loss filtering ...
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bioRxiv - Immunology 2020Quote: ... ~ 5 μL of the sonicated samples were drop cast onto 300-mesh formvar carbon-coated copper grids (Electron Microscopy Sciences, UK) and negatively stained with UranyLess (Electron Microscopy Sciences) ...
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bioRxiv - Systems Biology 2023Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS with 0.2% Triton X-100 and mounted onto slides with DAPI Fluoromount (#17984-24, Electron Microscopy Sciences) before visualization with an EVOS imaging system (Thermo Fisher Sci).
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on studs using silver paint and coated with 5 nm of Palladium (sputter coater EMS150TS; Electron Microscopy Sciences). Samples were imaged at 5 kV on a FEI Magellan 400 XHR Field Emission Scanning Electron Microscope at the Stanford Nano Shared Facilities.
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bioRxiv - Cancer Biology 2024Quote: ... and pellets were resuspended in a 5 ml volume of fixative master mix of Paraformaldehyde-Glutaraldehyde Solution Karnovsky’s Fixative (Electron Microscopy Sciences). Fixative solution was prepared with 16% paraformaldehyde solution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 µL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... was infused into the well of the chamber and covered with a 5-mm diameter glass coverslip (7229605, Electron Microscopy Sciences). The skin was sealed with VetbondTM Adhesive ...