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Citations for Electron Microscopy Sciences :
2151 - 2200 of 2293 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 3.2 μL of RNCs were incubated on plasma-cleaned 300-mesh holey carbon grids (C-flat R2/2, Electron Microscopy Science) for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μl of sample was loaded on freshly glow discharged 1.2-1.3 300 mesh C-flat™ grids without further support (Electron Microscopy Sciences). The Vitrobot chamber was set to 4 °C and 95% humidity and grids were prepared with a range of blotting time and force ...
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bioRxiv - Cancer Biology 2022Quote: ... Target retrieval was performed in citrate buffer at pH 6.0 for 15 minutes at 110°C under pressure and sections were transferred to Sequenza slide staining racks (Electron Microscopy Sciences). Sections were treated with Bloxall (Vector labs ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Microbiology 2022Quote: Bacteria were grown in Luria Broth liquid culture overnight at 37°C and immobilized on a Formvar grid (Electron Microscopy Sciences) for 1 min 30 sec and the samples were negatively stained with 0.5% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4 buffer) was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Microbiology 2022Quote: ... From this stock a 3.5 µl drop was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Plant Biology 2023Quote: ... The specimens were finally polymerised at 70°C for 72 h in flat embedding silicon molds (#70905-01, EMS, Hatfield, USA).
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with treatments for 20 min at 37°C and immediately fixed by addition of paraformaldehyde (Electron Microscopy Sciences) to 3.7% ...
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bioRxiv - Molecular Biology 2024Quote: ... a 3.5 μL drop was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA) with a 4 nm carbon coat ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were submerged in 1.5% uranyl acetate in absolute methanol at -90 °C for 2 days and infiltrated with HM20 Lowicryl resin (Electron Microscopy Sciences) over 2 days at -45 °C ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μL of each sample was applied to a Formvar carbon-coated copper grid (Electron Microscopy Sciences). Samples were incubated on the grid for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Ovarioles were separated from the muscle sheath using Dumont style 5 stainless steel forceps (Electron Microscopy Sciences) before fixation ...
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bioRxiv - Cell Biology 2024Quote: ... Larval fat bodies (FBs) or guts were dissected in PBS using Dumont #5 forceps (Electron Microscopy Sciences). All tissues were fixed in 4% paraformaldehyde for 20 min at room temperature and rinsed briefly in PBS prior to staining ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were cleared with xylenes for 5 minutes and coverslipped with DPX (Electron Microscopy Sciences, Hatfield, PA) mounting medium ...
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bioRxiv - Biophysics 2023Quote: ... that were glow-discharged for 5 s at 40 mA in an EMS100X glow discharge unit (EMS) and then blotted for 0.5-1 s (Blot Force −2 ...
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bioRxiv - Cell Biology 2024Quote: ... Larval fat bodies (FBs) or guts were dissected in PBS using Dumont #5 forceps (Electron Microscopy Sciences). All tissues were fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Developmental Biology 2024Quote: ... inflorescences were cut and dissected with a Dumont tweezer (Electron Microscopy Sciences, style 5, no. 72701-D) down to stage 9 ...
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bioRxiv - Immunology 2024Quote: ... Cells were rinsed twice in FACS buffer then fixed in 1% PFA (Electron Microscopy Sciences, 15714-5) for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... rats were deeply anesthetized with sodium pentobarbital (180 mg/kg; Fatal-Plus C IIN, Vortech Pharmaceuticals, Dearborn, MI) and perfused with paraformaldehyde (#15710, Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Neuroscience 2021Quote: ... The 40 μm cryosections were cut at -18°C and placed over glass slides treated with tissue capture pen (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Neuroscience 2021Quote: ... samples were placed on a Durcupan block with a flat surface and covered with ACLAR® 33 C Film (Electron Microscopy Science). Samples were let polymerize for 72 h at 60 °C.
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bioRxiv - Cell Biology 2022Quote: ... 3.5 µl of each sample was applied to the plasma-cleaned C-flat grids (Electron Microscopy Sciences, CF-1.2/1.3-4Au-50), pre-blotted and blotted with filter paper (Whatman 1 ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified virions at 1 × 1010 PFU mL-1 was applied to a 400-mesh C-flat holey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed with pre-warmed (37°C) PBS and permeabilized for 2 minutes using 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... At −50 °C samples were washed four times in ethanol for 30 minutes each before gradual infiltration with HM20 resin (Electron Microscopy Sciences). Polymerization of the resin was done with a UV light source and was carried out at −50 °C for 48 hours followed by post-polymerization at room temperature for 2 days.
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bioRxiv - Microbiology 2024Quote: ... Three to four microliters of rsVLP or rsVLP-compound-GSH mixture were applied to either glow-discharged C-flatTM holey carbon copper grids (product No. CF312, Electron Microscopy Sciences) for the PV1-SC6b yeast sample or Lacey carbon copper grids with an ultra-thin carbon support film (product No ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.4 buffer for P116 without the N-terminal domain (246–818)) was applied to a 45 s glow- discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Biophysics 2024Quote: ... 200 mM NaCl and 0 – 0.01% LMNG or 1 mM CHAPSO) was applied to a 45 s glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA) or R1.2/1.3 300 copper mesh quantifoil grid (Quantifoil Micro Tools ...
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bioRxiv - Biochemistry 2024Quote: CryoEM grids were prepared by applying 3.5 μL of 0.6 mg/mL NX11Q21.5-DNA48 in TBS with 2.5% glycerol to glow-discharged 400 mesh C-flat grids (CF-2/2-4C, 2 μm holes, 2 μm spacing; Electron Microscopy Sciences). The grids were blotted with a blot force of 0 for 6.5 seconds at 100% humidity and 20 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl of γ-tubulin mixture was pipetted onto EM grids (Electron Microscopy Sciences, Catalog number: CF400-Cu), which were glow discharged for 25 seconds ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were washed twice in MOPS buffer for 5 min and fixed in glutaraldehyde solution (EMS, Hatfield, PA) 2.5% in 100 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... RT) for 5 min and fixed with PFA (1.6%, Paraformaldehyde 16% solution EM grade, Electron Microscopy Sciences, 15710) before freezing ...
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bioRxiv - Neuroscience 2020Quote: ... Ribbons of ~5 sections were oriented and collected on formvar/carbon coated nickel slot grids (Electron Microscopy Sciences) using a perfect loop (Electron Microscopy Sciences).
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bioRxiv - Plant Biology 2022Quote: ... seedlings were washed twice in MOPS buffer for 5 min and fixed in glutaraldehyde solution (EMS, Hatfield, PA) 2.5% in 100 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed by adding a warm double strength fixative solution (5% glutaraldehyde (GA, Electron Microscopy Sciences, #16310) and 4% paraformaldehyde (PFA ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of protein sample was applied to freshly-ionized carbon-coated copper mesh grids (Electron Microscopy Science). After 15 minutes ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of each fixed sample was deposited onto carbon-coated copper grids (Electron Microscopy Sciences, CF400-Cu). Grids were UV-glow discharged immediately prior to sample addition ...
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bioRxiv - Developmental Biology 2024Quote: Ablation was done using a pair of sharpened dissecting forceps (Electron Microscopy Sciences, style 5, no. 72701-D) on stage 2 floral meristems on dissected inflorescences ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µm FFPE pancreas sections were mounted onto 22 mm x 22 mm glass coverslips (Electron Microscopy Sciences) coated in 0.1% poly-L-lysine (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... they were cut and dissected with a Dumont tweezer (Electron Microscopy Sciences, style 5, cat. no. 72701-D) to remove buds older than stage 9 or 10 ...
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bioRxiv - Neuroscience 2020Quote: ... rats were deeply anesthetized with sodium pentobarbital (180 mg/kg; Fatal-Plus C IIN, Vortech Pharmaceuticals, Dearborn, MI) and perfused with paraformaldehyde (#15710, Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μL of the sample was repeatedly applied and manually blotted three times onto 300 mesh R1.2/R1.3 C-flat Holey carbon grids (Electron Microscopy Sciences, Hatfield, PA, USA) using the multiple blotting technique prior to the Vitrobot step to increase the concentration of pili (34) ...
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bioRxiv - Biochemistry 2022Quote: ... sarkosyl and 4 μl of the suspension was applied directly to a glow-discharged C-flat™ Holey Carbon CF-2/2-4C Cu 400 mesh cryo-EM grid (Electron Microscopy Sciences) or Quantifoil R2/2 Cu 300 mesh grids in the chamber of the Leica GP2 plunging robot ...