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Citations for Electron Microscopy Sciences :
1951 - 2000 of 2018 citations for BN80933 CAS 214348 10 4 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... to be non-responsive to toe pinch and transcardially perfused with 10 ml of 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences (EMS)) to flush the vasculature followed by buffered fixative made of 2.5% glutaraldehyde (EMS) ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was washed with MQ water and stained for 20 min with 10 μl 0.1% uranyl formate solution (Electron Microscopy Sciences). The uranyl formate solution was prepared freshly and filtered with a 0.22 μm sterile syringe filter (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots (10 µL) of fixed cells were then loaded onto PTFE printed glass slides (cat#63429-04 from Electron Microscopy Sciences) with 4-mm diameter wells and allowed to air-dry ...
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bioRxiv - Cancer Biology 2024Quote: ... sample slides were washed 2 times in Staining Buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) storage buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Cell Biology 2024Quote: ... sample slides were washed 2 times in Staining Buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... sample coverslips were washed 2 times in staining buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 15 minutes each.The sections were then embedded in Epon-12 resin by sandwiching the sections between two sheetsof ACLAR (EMS 50425-10) and leaving them at 60 °C for 48 hours ...
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bioRxiv - Biophysics 2023Quote: ... one quarter of the bell from each medusae was chemically fixed in 10 mL of 2.5% [v/v] glutaraldehyde (Electron Microscopy Sciences), 4% [v/v] paraformaldehyde (Electron Microscopy Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM EDTA was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were again washed with deionized water five times for 10 min before staining with 0.2% (w/v) uranyl acetate (EMS, Cat# 22400) in water overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... mexicana were fixed directly in medium for 10 min at room temperature in 2.5% glutaraldehyde (glutaraldehyde 25% stock solution, EM grade, Electron Microscopy Sciences). Centrifugation was carried out at room temperature for 5 min at 16,000 g ...
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bioRxiv - Immunology 2024Quote: ... Sections were fixed in 10% formalin for 30 minutes at room temperature and incubated in Bouin’s fixative solution (Electron Microscopy Sciences) overnight at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Purified septa (10 μL) were added onto an UV-irradiated (16 h) formvar/carbon film-coated copper grid (Electron Microscopy Sciences) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were then embedded in Epon-12 resin by sandwiching the sections between two sheets of ACLAR (EMS 50425-10) and leaving them at 60 °C for 48 hr ...
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bioRxiv - Biophysics 2021Quote: Tau fibrils or tau fibrils-chaperone mixtures (10 μl) were deposited on glow discharged carbon-coated copper EM grids (Electron Microscopy Sciences), washed with three consecutive drops of 1% w/v Uranylformate ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
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bioRxiv - Cell Biology 2020Quote: Immuno-labeled sections on tape were attached to a 10 cm diameter silicon wafer (University Wafer, South Boston, MA) with double-sided carbon adhesive tape (Electron Microscopy Sciences). Wafers were carbon coated (Denton ...
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bioRxiv - Genomics 2021Quote: ... sectioned (thickness of 7-10 µm) and put at the center of poly-L-lysine covered glass slides (63478-AS, Electron Microscopy Sciences).
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Cancer Biology 2020Quote: ... and stomach were collected from Cre- and Cre+ mice and fixed in Modified Davidson’s fixative (64133-10, Electron Microscopy Sciences, PA, USA) for 48 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... washed and incubated for 1 h at room temperature with gold conjugated (10-nm) goat anti-rabbit-IgG (1:15, Electron Microscopy Sciences).
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bioRxiv - Cancer Biology 2021Quote: Fresh frozen optimal cutting temperature (OCT) compound-embedded tissues blocks were sectioned to 10 μm thickness onto RNAse-free Superfrost plus glass slides (Electron microscopy sciences) on a Leica CM1950 cryostat at −20°C ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with a secondary antibody conjugated with electron-dense colloidal gold nanoparticles of 10 nm size (Aurion, Electron Microscopy Sciences). GFP (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were cut at 180 nm and mounted on 15 mm glass coverslips coated with a Tissue Capture Pen (EMS, 71314-10). Sections were then embedded in VectaShield (Vector Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... to be non-responsive to toe pinch and transcardially perfused with 10 ml of 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences (EMS)) to flush the vasculature followed by buffered fixative made of 2.5% glutaraldehyde (EMS) ...
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bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Microbiology 2022Quote: ... Washed tardigrades were dried onto one field of a 76 × 26 × 1 mm 10-well (6mm) polytetrafluoroethylene-printed slide (Electron Microscopy Sciences). Fixation of specimens was performed by adding 30 μL of ice-cold 3:1 4% paraformaldehyde:1x phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the preparations were optically cleared in 25% glycerol for 1 hr at 25 °C and then mounted on Superfrost slides (Cat# 12-550-143, Fisherbrand) with bridged Glass Cover Slips #0 (Cat# 72198-10, Electron Microscopy Sciences) in Fluoromount-G mounting media (Cat# 0100-01 ...
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bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
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bioRxiv - Bioengineering 2023Quote: ... the grids were incubated in a drop of Goat-anti-Mouse IgG coupled with 10 nm gold nanoparticles (Electron Microscopy Sciences, 25512) diluted at 1:100 in PBS containing 0.1% BSA for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... from native aggregation assays sonicated in a water bath for 10-15 s were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 s using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... sections were dehydrated in a series of graded (25, 50, 75, 100, 100, 100 v/v%, 10 min each) acetonitrile (Electron Microscopy Sciences) aqueous solutions ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2022Quote: ... We then bound the chips to a 24×50×1.5mm glass slide (Fisherbrand 12-544-EP) after activating both surfaces for 10s at 100W in a plasma oven (EMS Quorum 1050X). We found that carefully optimizing plasma parameters and controlling humidity were critical for good binding ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were stained with 10% Uranyl acetate (Sisco Research Laboratory Pvt. Limited, 81405) for 5-min and Sato’s lead citrate (Electron Microscopy Sciences, 17800) stain for 1-min ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were washed five times for 10 min each with sterile deionized water and then transferred to fresh solution containing 1% (w/v) thiocarbohydrazide (EMS, Cat# 21900) in deionized water and incubated for 1 hour under rotation at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by heat induced antigen retrieval in a programmable antigen retrieval cooker (Aptum Bio Re-triever 2100) using 1X R-UNIVERSAL Epitope Recovery Buffer (EMS, #62719-10). The sections were then blocked with BLOXALL® Endogenous Alkaline Phosphatase Blocking Solution (VectorLabs ...
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bioRxiv - Systems Biology 2024Quote: ... snap frozen in OCT blocks and sectioned at a thickness of 7-10 μm and mounted on the center of poly-l-lysine-covered glass slides (63478-AS, Electron Microscopy Sciences). Mouse Whole Embryo Paraffin Sagittal Sections ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 20,000 seeds homozygous for the SCRpro::GFP-SCR transgene were treated overnight at room temperature with 10 mL 0.1% ethyl methanesulfonate (EMS, Sigma M0880, MO, USA). After thorough washing ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Neuroscience 2024Quote: Coverslips that were 22m x 22m and size #1 were used as the transparent walls of the chamber (Electron Microscopy Sciences: #72200-10). Rain-X was applied to the inner surface of the coverslips to limit flies from walking on the glass ...
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bioRxiv - Microbiology 2024Quote: ... the wells were fixed by adding on top of the supernatant 10% formaldehyde (Formaldehyde Aqueous Solution EM grade, 32%, Liquid Electron Microscopy Sciences, # 15700) at a volume-to-volume ratio for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Each explant was mounted onto an O-ring (McMaster Carr 1283N264) fixed into a 60mm dish (Cellstar 628160) with Sylgard 184 silicone elastomer (EMS Catalog #24236-10). Each region was imaged with a 25X objective and cells were counted on a maximal intensity projection ...
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bioRxiv - Cell Biology 2022Quote: ... 0.4 μm Pore Polyester Membrane Insert fixed to a custom 3D printed insert (Supplemental Material) with Sylgard 184 silicone elastomer (EMS Catalog #24236-10).