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Citations for Electron Microscopy Sciences :
1901 - 1950 of 2018 citations for BN80933 CAS 214348 10 4 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the coverslips were rinsed with 1X PBS and then fixed with freshly prepared 4% paraformaldehyde (from 32% Paraformaldehyde Solution, Electron Microscopy Science Cat# 15714-S) for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Bioengineering 2023Quote: ... mice were placed under anesthesia and tissues were perfused with 10mL of cold PBS and 5mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, #15710, Hatfield, PA). Brains were post-fixed overnight in 4% PFA at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... This concentrate was diluted 1:2 with molecular grade water and 50 µL was fixed with 25% glutaraldehyde (stored at 4°C, Electron Microscopy Sciences, Hatfield, PA, USA) to a final concentration of 0.25% ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were stained en bloc with 2% aqueous uranyl acetate overnight at 4° C in the dark (22400-2, Electron Microscopy Sciences, Hatfield, PA, USA). After staining ...
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bioRxiv - Genetics 2024Quote: ... i3LMNs were cultured in clear-bottom imaging 96-well plates and fixed with an equivalent volume of 4% paraformaldehyde (PFA, Electron Microscopy Sciences 50-980-487) in PBS added directly to the cell culture media ...
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bioRxiv - Biochemistry 2024Quote: ... 4 µl of sample were added to freshly glow-discharged 300 mesh 2/1 µm C-flat copper grids (Electron Microscopy Sciences, Hatfield, Pennsylvania, USA), blotted for 10 s at blot force 4 at 100% relative humidity and 4°C before being plunge frozen in a liquid mix of 37% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... The TLPs were adsorbed for 10 min on carbon-coated Parlodion films mounted on 300-mesh copper grids (EMS). Samples were washed once with water ...
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bioRxiv - Microbiology 2021Quote: ... and lysed by vortexing for 10 minutes with cell disruptor beads (0.1-mm diameter; Electron Microscopy Sciences, Hatfield, PA). Lysate was transferred to an 8ml glass vial with an additional 1ml cold methanol rinse of the lysis tube ...
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bioRxiv - Microbiology 2022Quote: ... The TLPs were adsorbed for 10 min on carbon-coated Parlodion films mounted on 300-mesh copper grids (EMS). Samples were washed once with water ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with 10 nm gold-conjugated goat anti-guinea pig IgG (1:200, Electron Microscopy Sciences, #25329) in blocking buffer for 3 hr ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed in D-PBS and treated with Sodium Citrate based-R-buffer A (EMS, #62706-10) in 2100-Retriever (EMS ...
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bioRxiv - Neuroscience 2023Quote: ... Sympathetic ganglia were whole-mounted to a slide with Fluoro-Gel with Tris Buffer (Electron Microscopy Sciences, 17985-10).
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bioRxiv - Cell Biology 2024Quote: ... Tissue was then rinsed in acetate buffer and dehydrated through a graded series of 10-minute ethanol rinses prior to infiltration in EmBED812 epoxy resin using the manufacturer’s hard resin formulation (EMS). Finally ...
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bioRxiv - Physiology 2023Quote: ... One microliter of this resuspension is added to 1 mL of Mammalian Ringer’s Solution (Electron Microscopy Sciences #11763-10) containing isolated myofibers from the flexor digitorum brevis muscle (FDB ...
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bioRxiv - Neuroscience 2023Quote: bdEV preparations (10 μL) were adsorbed to glow-discharged 400 mesh ultrathin carbon-coated grids (EMS CF400-CU-UL) for 2 minutes following our published protocol (Huang et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was then stained by adding 10 µL of 2% filtered uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Grids were then blotted dry with filter paper and stored at RT and visualized by using a bottom-mounted Eagle 4k x 4k camera on a Tecnai F20 TEM (FEI Company ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µL of solution was then applied to an individual PTFE Printed Slide well (Electron Microscopy Sciences, 63430-04) and incubated at room temperature for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... coli BioParticles™ conjugate or DQ™ Green BSA was added for 30min at 4°C and moved to Cytoseal mounting medium (Electron Microscopy Sciences, Hatfield, PA) set at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: Subcutaneous tumors were excised from flank skin including adjacent skin layers and then fixed in 4% PFA (16% PFA, Electron Microscopy Sciences, diluted in 1X PBS) at 4°C overnight on a rotator ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then washed and coverslipped with Fluoro-Gel Mounting Medium with Tris Buffer (catalog #17985-10, Electron Microscopy Sciences). Primary antibodies included in this study were ...
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bioRxiv - Immunology 2021Quote: ... and mounted on the slide using 10 μl of FLUORO-GEL II mounting medium with DAPI (EMS; Cat.# 17985-51) and visualized using an Axio Observer Z1 fluorescence microscope (Zeiss) ...
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bioRxiv - Physiology 2020Quote: ... a few 10 µm thick sagittal cryosections were collected on adhesive slides (Plus gold slide, Electron Microscopy Sciences, Hatfield, PA) and stored for up to 30 min in ice-cold PBS prior to AFM analysis.
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bioRxiv - Neuroscience 2020Quote: ... 10 μl of fibril sample (50 μM) was spotted on carbon-coated Formvar EM grids (Electron Microscopy Sciences, PA, USA), incubated for 5 mins and stained with uranyl formate (1% v/v) ...
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bioRxiv - Biochemistry 2020Quote: 5 μL of purified sample was applied to glow-discharged (10s) Quantifoil R 1.2/1.3 grids (Au 400; Electron Microscopy Sciences) and plunge-frozen in liquid nitrogen-cooled liquid ethane ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of the sample was applied to glow-discharged (10 s) Quantfoil R1.2/1.3 holey carbon grids (Au 400, Electron Microscopy Sciences) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μL of samples were mounted on Formvar/carbon film-coated mesh nickel grids (Electron Microscopy Sciences, Hatfield, PA, USA) and left standing for 2 min ...
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bioRxiv - Neuroscience 2020Quote: EV preparations (10 µL) were adsorbed to glow-discharged 400 mesh ultra-thin carbon coated grids (EMS CF400-CU-UL) for two minutes ...
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bioRxiv - Microbiology 2022Quote: EV preparations (10 μL) were adsorbed to glow-discharged 400 mesh ultra-thin carbon-coated grids (EMS CF400-CU-UL) for 2 min followed by 3 rinses in TBS and staining in 1% uranyl acetate with 0.05 Tylose ...
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bioRxiv - Cell Biology 2022Quote: ... After washing with 1XPBS for 10 min the stained guts were mounted in Fluoromount-G Mounting Medium (Electron Microscopy Sciences). Stained posterior midguts were imaged in the R5 region using LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... Subsequently, sections were incubated in DAPI (1:1000, 10 mins) and mounted with Fluoromount G (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed and coverslipped with Fluoro-Gel Mounting Medium with Tris Buffer (catalog #17985-10, Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2023Quote: ... Antigens were retrieved through immersion in 10 mM sodium citrate buffer (0.05% Tween 20, pH 6.0) in a pressure cooker (Retriever 2100, Electron Microscopy Sciences) at 120 °C for 20 min and cooled to room temperature for 100 min ...
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bioRxiv - Bioengineering 2023Quote: Purified DNA origami structures (1–10 nM) were adsorbed onto freshly glow discharged copper grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 4 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Microbiology 2024Quote: 4 tubes of 10 mL each were inoculated overnight for approximately 18 hours (or the 40 mL of culture from EMS (-) strains were used) ...
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bioRxiv - Microbiology 2024Quote: ... Assembly samples (10 μL) were applied to glow-discharged formvar/carbon 300-mesh Cu grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 2 min ...
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bioRxiv - Physiology 2024Quote: Atrial and ventricular tissue samples were cut into pieces of about 1mm3 and fixed in a mixture of 2% glutaraldehyde (16316-10, EMS) and 2% formaldehyde (15700 ...
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bioRxiv - Microbiology 2024Quote: Assembly samples (10 μL) were applied to glow-discharged formvar/carbon 300-mesh Cu grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 2 min ...
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bioRxiv - Biochemistry 2024Quote: ... AFM samples were prepared by adsorbing 10 μl of 5 times diluted sample onto mica (Muscovite mica, V 1 quality, EMS) for 45 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... coronal cryosections of 10 μm were prepared on poly-L-lysine-coated glass slides (no. 63478-AS, Electron Microscopy Sciences) or 50×75 mm poly-L-lysine-coated glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and the pellet was fixed in 2.5% glutaraldehyde and 4% PFA (16% stock solution, EM grade, Electron Microscopy Sciences in 0.1 M PIPES (pH 7.2)) for minimum 2h ...
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Cortical synaptic vulnerabilities revealed in a α-synuclein aggregation model of Parkinson’s diseasebioRxiv - Neuroscience 2024Quote: ... The slides were dried at RT and embedded in mounting media freshly prepared by mixing Tris-MWL 4-88 (Electron Microscopy Sciences #17977-150, Hatfield, PA, USA) with AF300 (Electron Microscopy Sciences #17977-25 ...
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bioRxiv - Bioengineering 2024Quote: ... Uterine horn cross sections (two per horn per mouse) were excised and fixed in a 4% (v/v) formaldehyde solution (Electron Microscopy Sciences, 32% aqueous solution, Cat# 100496-496) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on bone slices for 3 days were unroofed as described above and fixed for 10 min in a 0.2M sodium cacodylate buffer (pH 7.4) containing 2% paraformaldehyde (Electron Microscopy Sciences 15710) and 2.5% glutaraldehyde (Electron Microscopy Sciences 16220) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µL of resuspended cells were pipetted onto agarose pads in 13 x 1 mm silicone isolator wells (Electron Microscopy Sciences) and covered with a No ...
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bioRxiv - Biophysics 2020Quote: For TEM imaging 10 μL of recombinant tau fibril samples were applied to copper grid (Electron Microscopy Science, FCF-300-Cu) cleaned with plasma for 20 s ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μl of diluted sample was deposited onto a glow-discharged (Edwards S150B, 30sec, 30-50 mA, 1,2kV, 10-2 mbar) continuous carbon grid (CF400-CU-UL, Electron Microscopy Sciences). After one minute at RT ...