Labshake search
Citations for Electron Microscopy Sciences :
1801 - 1850 of 3351 citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: The kidneys containing the grafts were preserved by in vivo intracardiac perfusion in anesthetized mice with heparinized saline buffer (pH 7.4) followed by 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA, USA), and then immersed for 24 hours at 4 °C in the same fixative agent ...
-
bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion was done with a prewash with 9.25% sucrose in distilled water and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion TwoTM Automated Pressure Perfusion System (Leica Biosystems ...
-
bioRxiv - Neuroscience 2023Quote: ... rats were transcardially perfused with an isotonic solution at 9.25% in distilled water as a prewash and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion Two TM Automated Pressure Perfusion System (Leica Biosystems ...
-
bioRxiv - Biophysics 2023Quote: ... Grids were made using a Vitrobot™ Mark IV (FEI) at 4 °C and 100 % humidity with Grade 595 Vitrobot™ filter paper (Electron Microscopy Sciences). All grids were plunge frozen using liquid ethane and stored in liquid nitrogen.
-
bioRxiv - Developmental Biology 2023Quote: Gels were fixed by 30 minutes rotation incubation at room temperature in freshly prepared 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA, Cat# 15710S) diluted in PBS-T ...
-
bioRxiv - Developmental Biology 2024Quote: ... and pancreas were harvested at the experimental endpoint and immediately fixed in 4% paraformaldehyde in 1X PBS (16% solution, Electron Microscopy Sciences, cat# 15711) at 4°C overnight ...
-
bioRxiv - Developmental Biology 2023Quote: ... or any of the four possible distributions at the 4-cell stage (ABa and P2; ABa and EMS; ABp and P2; ABp and EMS), were more likely to harbor sperm-contributed centrioles using the χ2 test as implemented in the library SciPy ([https://scipy.org/citing-scipy/]) ...
-
bioRxiv - Neuroscience 2024Quote: ... 240 mg/kg body weight) or on ice (for early postnatal animals) and transcardially perfused with PBS followed by ice-cold 4% paraformaldehyde (EMS #15714, diluted in PBS). Spinal cords were removed and post-fixed for 2 hours (neonatal spinal cords ...
-
bioRxiv - Neuroscience 2024Quote: ... animals were anesthetized in 0.02% MS-222 and then immersed in 4% PFA (diluted from 16% PFA in 1xPBS, Cat # 15710, Electron Microscopy Sciences, Fort Washington, PA) using a Pelco Biowave Pro microwave (Model 36700 ...
-
bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and then perfused transcardially with room temperature (RT) phosphate buffered saline (PBS) with heparin (0.02 mg/mL) and then ice-cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, catalog no: 15714). Brains were dissected and post-fixed in 4% PFA overnight at 4°C and cryopreserved in 30% sucrose in PBS for ∼2 days at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... fixed for 20 min in 4% EM-grade paraformaldehyde (PFA) diluted in phosphate-buffered saline (PBS) from an 8% PFA Aqueous Solution (Electron Microscopy Sciences, 157-8) and blocked for 1 h in PBS plus 0.2% Triton X-100 and 4% normal goat serum (NGS ...
-
bioRxiv - Cell Biology 2024Quote: ... was added to the 96-well co-culture model at day 5 with a final concentration of 5 µM in the appropriate medium (NF, EMS, or LMS) and incubated for 30 min at 37°C ...
-
β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... 1 mL of culture was fixed at ambient temperature in the dark (1-2 h) with paraformaldehyde 3.8% (PFA, Electron Microscopy Sciences). Cells were then centrifuged 3 min at 8000 g and washed 3 times with 500 µL PBS ...
-
bioRxiv - Neuroscience 2023Quote: ICC(3, 1) = (BMS-EMS)/(BMS+(k-1)*EMS),
-
bioRxiv - Immunology 2024Quote: ... Cells were rinsed twice in FACS buffer then fixed in 1% PFA (Electron Microscopy Sciences, 15714-5) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 mm coverglass (Electron Microscopy Sciences, Ref. 72195-05).
-
bioRxiv - Microbiology 2023Quote: ... and counter stained with saturated 5% uranyl acetate (EMS), followed by Reynold’s lead citrate (EMS) ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains from adult females were dissected in Phosphate-buffered saline (PBS, Eurobio, ref CS0PBS0108) and fixed for 15 min in 4% paraformaldehyde (Electron Microscopy Sciences, Cat # 15714-S) at room temperature (RT) ...
-
bioRxiv - Genetics 2020Quote: ... Then the cells were counted and diluted to 106 cells/ml and fixed with 4% paraformaldehyde aqueous solution (Electron Microscopy Sciences #15710, Formaldehyde methanol-free) for 10 minutes at room temperature (RT) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The eye imaginal discs were dissected in cold 1X solution of Phosphate Buffered Saline (PBS) and fixed using 4% p-formaldehyde (Electron Microscopy Sciences, Catalog no. 15710). Fixed tissues were washed using PBS-T [0.2% Triton X-100 (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... the cultured neurons were fixed for 10-15 min by transferal of the coverslip into a dish with 4% paraformaldehyde (16% aqueous solution, 15710; Electron Microscopy Sciences, Hatfield, Pennsylvania, USA) and 0.2% glutaraldehyde (25% aqueous solution ...
-
bioRxiv - Bioengineering 2020Quote: Animals were implanted with a stainless-steel probe or a Fiber F1/F2 for 4 weeks and then anesthetized with a ketamine (20 mg/mL)/xylazine (2 mg/mL) solution and transcardially perfused with phosphate-buffered saline (PBS) (Fisher BP661-10) followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Cat. #15714-S) in PBS ...
-
bioRxiv - Genomics 2020Quote: ... cells were fixed by adding the fixative directly to the medium to a final concentration of 4% paraformaldehyde (PFA) (Electron Microscopy Sciences; cat. no. 15714) using the automated liquid handler BlueWasher (BLUE CAT BIO) ...
-
bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
-
bioRxiv - Neuroscience 2023Quote: ... the coverslips were rinsed with 1X PBS and then fixed with freshly prepared 4% paraformaldehyde (from 32% Paraformaldehyde Solution, Electron Microscopy Science Cat# 15714-S) for 30 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... mice were placed under anesthesia and tissues were perfused with 10mL of cold PBS and 5mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, #15710, Hatfield, PA). Brains were post-fixed overnight in 4% PFA at 4°C ...
-
bioRxiv - Genetics 2024Quote: ... i3LMNs were cultured in clear-bottom imaging 96-well plates and fixed with an equivalent volume of 4% paraformaldehyde (PFA, Electron Microscopy Sciences 50-980-487) in PBS added directly to the cell culture media ...
-
bioRxiv - Immunology 2022Quote: ... The sample was stained by 6 µL 2% uranyl acetate solution (Electron Microscopy Sciences, Catalog number 22400-2) twice ...
-
bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were then infiltrated for 1 h with 1:1 PO:Embed 812 (EMS), and then overnight in 1:2 PO:Embed 812 ...
-
bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
-
bioRxiv - Plant Biology 2024Quote: ... Samples of approximately 5×5 mm were attached to a sample holder using a thin layer of Tissue-Tek Compound (EMS, Washington, PA, USA). The sample holder was then frozen in liquid nitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... The slice was then left to rest in the recording chamber for 4-5 minutes to ensure transport of biocytin to distal dendrites and axon processes (Swietek et al., 2016)39 before the slice was moved into 4% PFA (Electron Microscopy Sciences, CAS #50-00-0) diluted in 0.1M Dulbecco’s Phosphate Buffered Saline (PBS ...
-
bioRxiv - Immunology 2023Quote: ... coli BioParticles™ conjugate or DQ™ Green BSA was added for 30min at 4°C and moved to Cytoseal mounting medium (Electron Microscopy Sciences, Hatfield, PA) set at 37°C with 5% CO2 ...
-
bioRxiv - Bioengineering 2024Quote: Subcutaneous tumors were excised from flank skin including adjacent skin layers and then fixed in 4% PFA (16% PFA, Electron Microscopy Sciences, diluted in 1X PBS) at 4°C overnight on a rotator ...
-
bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
-
bioRxiv - Biophysics 2024Quote: ... The grids were negatively stained for one minute using 3.5 µL of 3% uranyl acetate (Electron Microscopy Sciences). Excess liquid was blotted off using filter paper and the grids were dried overnight before imaging ...
-
bioRxiv - Cell Biology 2024Quote: ... culture aliquots were taken and mixed 1:2 with Paraformaldehyde 3% Glutaraldehyde 0.35% In 0.1M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Cells were fixed for 30-60 min at RT and diluted in low fluorescence media ...
-
bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
-
bioRxiv - Cell Biology 2022Quote: ... The subcellular localization of 5 nm BSA Nanogold tracer (EMS), was determined from images taken at minimum magnification of ×10,000 ...
-
Cortical synaptic vulnerabilities revealed in a α-synuclein aggregation model of Parkinson’s diseasebioRxiv - Neuroscience 2024Quote: ... The slides were dried at RT and embedded in mounting media freshly prepared by mixing Tris-MWL 4-88 (Electron Microscopy Sciences #17977-150, Hatfield, PA, USA) with AF300 (Electron Microscopy Sciences #17977-25 ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and the pellet was fixed in 2.5% glutaraldehyde and 4% PFA (16% stock solution, EM grade, Electron Microscopy Sciences in 0.1 M PIPES (pH 7.2)) for minimum 2h ...
-
bioRxiv - Cell Biology 2021Quote: ... and post-fixed for one hour with 2% osmium tetroxide (Electron Microscopy Science, USA, cat. 19190) in 0.15 M of phosphate buffer ...
-
bioRxiv - Cell Biology 2020Quote: Cells were cultured on cell tight C-2 cell disks (Sumitomo Bakelite) and fixed for 2 h in 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M phosphate buffer (pH 7.4 ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
-
bioRxiv - Biophysics 2024Quote: ... This cell suspension (3 µl) was applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) that were glow discharged for 30 seconds at 15 mA ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were fixed at 18 hpi for 2 h on ice in 2.5% glutaraldehyde (EMS) in 0.1 M cacodylate buffer (CB ...
-
bioRxiv - Cell Biology 2022Quote: ... 2 h in the dark in 0.5% (w/v) uranyl acetate (EMS, Hatfield, PA, USA) dissolved in 0.05 N maleic acid buffer (pH 5.2) ...
-
bioRxiv - Bioengineering 2024Quote: ... Uterine horn cross sections (two per horn per mouse) were excised and fixed in a 4% (v/v) formaldehyde solution (Electron Microscopy Sciences, 32% aqueous solution, Cat# 100496-496) in PBS ...
-
bioRxiv - Biophysics 2023Quote: ... 3 μL of the sample was applied to glow-discharged (45 s) Quantifoil (2/2; Electron Microscopy Sciences) grids ...