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Citations for Electron Microscopy Sciences :
1651 - 1700 of 3351 citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Whole kidney organoids and mouse kidney explants were fixed for 20 min on ice in 4% PFA (Electron Microscopy Sciences, 15710) in 1xPBS ...
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bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with ice-cold phosphate buffer saline (PBS) followed by PBS containing 4% paraformaldehyde (PFA) (Electron Microscopy Sciences). Brains were extracted ...
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bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... then fixed for 20 minutes at room temperature (RT) in a fixative comprised of 4% (v/v) paraformaldehyde (Electron Microscopy Sciences), 25% (v/v ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature with 4% PFA (Electron Microscopy Sciences) diluted in PBS and permeabilized with 70% ethanol overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... either immediately snap-freezing in tubes on dry ice or being immediately immersed in 4% paraformaldehyde (Electron Microscopy Sciences, 15714-S) diluted in PBS for fixation overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then transferred into an ice filled bath and washed 4× for 30 min each with acetone and infiltrated with Spurr’s resin (Electron Microscopy Sciences). During final steps of resin infiltration ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Tissues were pooled and fixed in a fixation buffer (100mM Tris pH7.5, 300mL NaCl, 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA)) for 20min on a rotation platform set to a gentle speed at a frequency of 0.33Hz ...
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bioRxiv - Genomics 2024Quote: We selected the 5861 promoters accessible in the somatic cells of 4-cell stage embryos (earlyEMS, EMS and ABx cell types), and measured and visualised the ATAC-seq signal from adult germline cells (Serizay et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were placed on TEM one-slot grids (Agar Scientific, France) coated with Formvar film and stained 20 minutes with 5% uranyl acetate (Electron Microscopy Science, Hatfield, PA, USA) and 5 minutes Reynolds lead citrate ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed 3 × 5 minutes in PBS and mounted to slides using FluoroGel with DABCO (Electron Microscopy Sciences, #17985) and 1.5 thickness coverglass (Fisherbrand ...
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bioRxiv - Microbiology 2024Quote: Clinical samples and biofilm samples fixed using the SEM fixation buffer were subsequently subjected to a series of washing steps (3 repetitions for 5 mins each) with 0.1M Cacodylate buffer pH 7.4(Electron Microscopy Sciences, USA) and distilled water (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... subtilis colonies were inoculated into 5 mL of LB medium supplemented with 5 µL of ethyl methanesulfonate (EMS), and cultured at 37°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (Electron Microscopy Sciences, #17985-10) and viewed by confocal microscope (Leica SP8 ...
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bioRxiv - Immunology 2023Quote: ... Negative staining was performed for one minute using 2% Uranyl Acetate (EMS). The phage and bound antibody structures were visualized using JEM-1400 (Jeol ...
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bioRxiv - Developmental Biology 2024Quote: ... Dumont tweezers (Style 5, Electron Microscopy Sciences) were used ...
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bioRxiv - Bioengineering 2022Quote: ... 5% normal donkey serum (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 mm (Electron Microscopy Sciences, 7229605) glass coverslips were prepared by plasma etching with oxygen for 1 minute (GLOW Plasma System ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed once with 1X DPBS(-) and fixed with 4% PFA diluted from a freshly opened 16% PFA (Electron Microscopy Sciences) ampoule ...
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bioRxiv - Developmental Biology 2021Quote: SMG organoid and cell cultures grown on Nuclepore filters (Cytiva #110405) or glass were fixed with 4% paraformaldehyde (Electron Microscopy Sciences #15710) in 1xPBS for 20 minutes or in -20°C methanol for 18 minutes ...
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bioRxiv - Microbiology 2021Quote: ... infected or treated as noted for each experiment and fixed for 10 minutes at room temperature in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Migrated cells attached to the lower surface of the inserts were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 2 min ...
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bioRxiv - Microbiology 2022Quote: Cells were seeded onto coverslips for immunofluorescence experiments and fixed for 10 mins at 37 °C in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences). Samples were permeabilized with 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: Cells grown on glass bottom dishes (35 mm, 14 mm glass, uncoated, Mat-Tek Corporation) were fixed with 4% Paraformaldehyde (Electron Microscopy Sciences) in 1 M HEPES (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown on 35 mm Glass Based Dishes or 24-well EZVIEW Glass Bottom Culture Plates LB (IWAKI) and fixed with 4% PFA (Electron Microscopy Sciences) for 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed with PBS (not containing Mg2+ and Ca2+) thrice and fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) in PBS for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown on glass coverslips with or without collagen (invadopodia formation assay) or gelatin-coated coverslips (degradation assay) and fixed with 4% paraformaldehyde/PBS (Electron Microscopy Sciences). For the invadopodia assay on collagen-coated coverslips ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue-HDMI-array sandwich was moved to room temperature, and the tissues were fixed in 4% formaldehyde (100 μl, diluted from the EM-grade 16% paraformaldehyde (#15170, Electron Microscopy Sciences)) for 10 min.
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bioRxiv - Cell Biology 2022Quote: ... then fixed for 10 min at room temperature with pre-warmed 4% Paraformaldehyde solution in DPBS (37°C; prepared from 16% PFA (Electron Microscopy Sciences)) ...
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bioRxiv - Physiology 2022Quote: ... Adult fish were cardiacally perfused with a solution of 4% paraformaldehyde (PFA) diluted in phosphate buffered saline (PBS) solution (Electron Microscopy Sciences) to remove autofluorescence from blood cells40 ...
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bioRxiv - Neuroscience 2021Quote: At the end of experiments mice were perfused with phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science, USA) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were filleted on sylgard plates in room temperature (RT) PBS and fixed on a shaker in 4% paraformaldehyde (Electron Microscopy Sciences, EMS) in PBS for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... infected cells were washed twice with PBS buffer (pH 7.4) and fixed with a 4% methanol-free paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2020Quote: The selected MC12 cell lines were plated on a 35-mm glass bottom dish (IWAKI) and fixed with 4% formaldehyde (Electron Microscopy Sciences) in 250 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: Neurons were rinsed 1X with PBS at room temperature and then fixed for 15 minutes at room temperature with 4% paraformaldehyde (Electron Microscopy Sciences) made up in PBS and supplemented with 4% sucrose ...
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bioRxiv - Neuroscience 2020Quote: ... plated in glass bottom dishes or hESC-derived striatal neurons on coverslips were washed in D-PBS and fixed for 10 min in 4% PFA (Electron Microscopy Sciences). The cells were washed 3 times with D-PBS and incubated with DAPI (4′,6-diamidino-2-phenylindole ...
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bioRxiv - Physiology 2021Quote: ... whole heads or enucleated eyes were fixed for 20 minutes on ice in 4% PFA prepared in PBS from 20% stock solution (15713, Electron Microscopy Sciences). The samples were rinsed with PBS and stored in 100% methanol at −20°C ...
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bioRxiv - Immunology 2020Quote: ... Naive T cells were maintained 4 days in IL-7-supplemented medium and then fixed overnight in 2.5% glutaraldehyde (Electron Microscopy Sciences, UK) at 4°C and post-fixed with 1% osmium tetroxide (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2021Quote: ... the entire liquid medium was gently discarded from each dish and the adherent cells were immediately fixed with 4% PFA (Electron Microscopy Sciences), 0.1% glutaraldehyde (G5882 ...
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bioRxiv - Neuroscience 2021Quote: ... the patch pipette was gently removed from the recorded PC and the slice was immediately transferred from the recording chamber to a fixation solution composed of 4% paraformaldehyde (Electron Microscopy Sciences) in ACSF for a maximum of 24h (4°C).
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bioRxiv - Cell Biology 2023Quote: ... and PFF treatment was conducted at the indicated concentrations and durations prior to fixing cells with 4% Paraformaldehyde (EMS catalog 15710-S) in DPBS (Corning catalog ...
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bioRxiv - Developmental Biology 2023Quote: ... Adherent cells (at ESC stage for the CRISPRa validations and PP stage for the CRISPRi validations) were rinsed once with PBS with Ca2+ and Mg2+ (PBS++) and fixed with 4% Paraformaldehyde (Electron Microscopy Sciences 32% Paraformaldehyde ...
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bioRxiv - Immunology 2023Quote: FACS-sorted naïve CD4+ T cells from LNs and spleen were pelleted at 400 g for 4 mins by swinging bucket Eppendorf 5810 into BEEM conical capsule tips (EMS, #69913-05), and fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (CB ...
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bioRxiv - Neuroscience 2022Quote: ... The animals were then transcardially perfused with PBS and then with 4% paraformaldehyde (PFA) (diluted into 1xPBS from 16% PFA, Electron Microscopy Sciences), followed by post-fixation with 4% PFA for immunohistochemistry or Karlsson and Schultz (KS ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was carefully removed and cells were fixed for 10 minutes at room temperature with 50 µl of 4% paraformaldehyde freshly prepared from a 16% stock (Electron Microscopy Sciences). Cells were again washed with 800 µl PBS with 1% BSA and pelleted ...
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bioRxiv - Biochemistry 2024Quote: To generate negative-stain grids 4 µl of the GraFix fraction was applied to a Copper 400 Mesh grids with continuous carbon (Electron Microscopy Sciences) after glow discharge ...