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Citations for Electron Microscopy Sciences :
1551 - 1593 of 1593 citations for 1 2 Chloropyridin 3 yl 3 3 dimethylazetidin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... hTERT-RPE-1 and worm samples were washed three times with pure anhydrous acetone and infiltrated with Epon/Araldite (EMS, Hatfield, USA) at increasing concentrations of resin (resin:acetone ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were put in propylene oxide for 1 h and infiltrated overnight in a 11 mixture of propylene oxide and Spurr’s low viscosity resin (Electron Microscopy sciences, Hatfield, PA). The cells were embedded in Spurr’s resin and polymerized at 60°C for 48 h ...
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bioRxiv - Bioengineering 2020Quote: ... kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI) made of 1% OsO4 (Electron Microscopy Sciences, 19190), 1.25% potassium hexacyanoferrate (II ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in 100 mM sucrose and the cell suspension distributed onto slides (Matsunami, S9901) covered with 1% PFA in H2O (Electron Microscopy Science, 15713) with 0.2% Triton X-100 (pH 9.2-9.4) ...
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bioRxiv - Neuroscience 2021Quote: ... Neurobasal media was carefully aspirated from all wells and coverslips were briefly rinsed with ice cold 1× PBS with Ca2+/Mg2+ followed by fixation in freshly made 4% PFA (Electron Microscopy Sciences: 15713) in 1x PBS for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... 3 mL of cells was harvested by centrifugation for 3 min at 1500 × g at room temperature and fixed for 30 min in 1 mL of 2.5% (v/v) glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) in 0.1 M cacodylate buffer (Electron Microscopy Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (46) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... 6-8 μl of 1 nM DNA origami sample was wicked onto a glow-discharge-cleaned copper grid (Electron Microscopy Sciences, Hatfield, PA) and incubated for 5-10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were suspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% monomeric formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29) ...
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bioRxiv - Neuroscience 2024Quote: Coverslips that were 22m x 22m and size #1 were used as the transparent walls of the chamber (Electron Microscopy Sciences: #72200-10). Rain-X was applied to the inner surface of the coverslips to limit flies from walking on the glass ...
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bioRxiv - Biophysics 2023Quote: ... samples were taken directly from the microplates (section 2.3) and deposited for 1 min on a previously negative glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, Pennsylvania). The grids were then washed with water and stained with phosphotungstic acid ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with ice-cold 1% phosphatase inhibitor cocktail/PBS once and then fixed with freshly prepared 4% PFA (Electron Microscopy Science, 15710) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: 5 μl of PFF solution (∼1 mg/mL) was spotted on a freshly glow-discharged 300-mesh carbon coated formvar grid (Electron Microscopy Sciences, USA) and incubated for 3 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed for 1 hour at room temperature (RT) in Paraformaldehyde (PFA) 4% in PBS 1X (32% stock, Electron Microscopy Sciences, 15714). They were washed 4 times for 10min in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... Melatonin stock solutions of 0.1 mM or 0.01 mM (for a final concentration of 1 nM) were prepared by adding melatonin (Selleckchem, Catalog No. S1204, 10mM) to DMSO (Electron Microscopy Sciences 13390). To each dish containing 50 ml of fish water ...
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bioRxiv - Neuroscience 2024Quote: ... Neuronal cell number was determined and 1.0-1.25×105 cells were plated on 1 mm glass coverslips (Electron Microscopy Sciences; Cat. #: 72290-03) in a 24-well plate (Falcon ...
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bioRxiv - Biochemistry 2024Quote: ... followed by post-fixation in 1% osmium tetroxide in 0.1 M cacodylate buffer and embedding in Embed 812 Resin (Electron Microscopy Sciences, Hatfield, PA). One-µm wide sections were sliced on a Leica RM2265 rotary microtome equipped with a diamond knife ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Bioengineering 2022Quote: ... 4μL of ~1 nM purified DNA nanostructure was deposited onto a copper TEM grid coated with carbon and formvar (Electron Microscopy Sciences, Hartfield, PA) and incubated for 4 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were negatively stained with 1% uranyl acetate in Tris NaCl refolding buffer and fixed onto 400 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA). TEM imaging was done at 100x magnification with a JEOL 1400 electron microscope ...
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bioRxiv - Cell Biology 2021Quote: ... a film of germanium (Structure Probe, Inc., West Chester, PA) was applied to glass coverslips of 25 mm diameter and thickness #1 (Electron Microscopy Sciences, Hatfield, PA) as previously described (Heckman et al. ...
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bioRxiv - Cell Biology 2021Quote: ... were adsorbed for 30 s (for high salt conditions) to 1 h in a humid chamber (for low salt conditions) on a glow-discharged carbon-coated grid (Electron Microscopy Sciences, CF300-CU). For low salt conditions ...
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bioRxiv - Biochemistry 2022Quote: ... for four hours at room temperature (ref: “C. elegans: a practical approach”, chapter “Conventional Genetics” by Jonathan Hodgkin, “Protocol 1-EMS Mutagenesis”, pages 252-253) and selected for increased survival NGM-OP50-1 plates with on 5 mM −7.5 mM DTT ...
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bioRxiv - Systems Biology 2022Quote: ... 6F- Volume was completed to 8 ml with room temperature 1 X PBS and then 500 μl of 16 % PFA solution (Electron Microscopy Sciences, cat. # 15710) was added ...
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bioRxiv - Microbiology 2021Quote: ... Surface colonization was performed by washing and diluting an LB overnight culture 1:100 into fresh SCFM and dispensing 100 µl into coverslip attached open incubation chambers (Electron Microscopy Sciences, 70333-42). The coverslips were incubated in parafilm sealed sterile petri dishes at 37°C and the media was gently exchanged every 4 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and placed overnight in a 1:50 dilution of donkey anti–rabbit IgG with bound 10-nm colloidal gold (Electron Microscopy Sciences, catalog 25704) diluted in blocking buffer ...
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bioRxiv - Biochemistry 2024Quote: Purified receptor was diluted to ∼5 ug mL−1 and applied to freshly glow-discharged EM carbon coated copper grid (Electron Microscopy Sciences, CF400-Cu,) for 30 sec ...
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bioRxiv - Microbiology 2023Quote: ... We then immediately applied the mixture to glow-discharged Quantifoil grids (R 0.6/1, 300 mesh, gold, Electron Microscopy Sciences [EMS] Cat #: Q350AR-06) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... We then immediately applied the mixture to glow-discharged Quantifoil grids (R 0.6/1, 300 mesh, gold, Electron Microscopy Sciences [EMS] Cat #: Q350AR-06) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Five µL of OMVs that had been diluted 1:10 in saline were added to a glow discharged 200 mesh formvar/carbon grid (Electron Microscopy Sciences, FCF200-Cu) and incubated for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Cat# 70011044) and fixation with 4% paraformaldehyde (PFA) (dilution 1:4; Paraformaldehyde 16% Aqueous Solution EM Grade, Electron Microscopy Sciences, Hatfield, PA; Cat# 15710) were performed 6 hours after saline or LPS injection ...
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bioRxiv - Neuroscience 2024Quote: ... at the time of brain microdissection the spinal cord was rapidly dissected and immersed in 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA). For assessment of phospho-cJUN and ATF3 expression animals were perfused with 4% PFA ...
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bioRxiv - Neuroscience 2024Quote: ... i.p.) and transcranial perfused with 1 × ice-cold phosphate-buffered saline (PBS) followed by 4% ice-cold paraformaldehyde (PFA, Electron Microscopy Sciences, Pennsylvania, USA) made in PBS ...
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bioRxiv - Cell Biology 2021Quote: Samples were fixed in mixture of 4% paraformaldehyde (TAAB Laboratories Equipment Ltd, United Kingdom, cat. F/P001) and 1% glutaraldehyde (Electron Microscopy Science, USA, cat. 16310) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... Devices were fabricated by thoroughly mixing polydimethylsiloxane (PDMS) and a curing agent in a 10:1 ratio by weight (Sylgard 184, Electron Microscopy Sciences Cat. 24236 – 10), degassing the mixture under vacuum ...
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bioRxiv - Plant Biology 2020Quote: ... After 1 hour the leaf discs were washed three times with sterile water and then mounted in Fluoromount-G ™ (Electron microscopy Sciences, Hatfield, PA, USA). Leaf discs were imaged with a Zeiss LSM800 Airyscan laser scanning confocal microscope (Carl Zeiss MicroImaging ...
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bioRxiv - Neuroscience 2020Quote: ... The midbrain and eyestalk neural tissue was dissected free from the exoskeleton and its enveloping sheath in ice-cold chromating solution comprising 1 part 25% glutaraldehyde (Electron Microscopy Sciences; Cat# 16220; Hatfield, PA), 5 parts 2.5% potassium dichromate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated overnight at 4°C in a 1:100 dilution of Goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8 nm colloidal gold (Aurion, Electron Microscopy Sciences, Fort Washington, PA, cat #25121-0.6ml). Following washes in IEM buffer ...