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Citations for Electron Microscopy Sciences :
1451 - 1500 of 1593 citations for 1 2 Chloropyridin 3 yl 3 3 dimethylazetidin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... following by a 1-hour incubation in an Aurion Goat Blocking Solution (Aurion, Electron Microscopy Sciences, Fort Washington, PA). The cells were incubated in anti-APOE antibody overnight at 4°C (Rabbit anti-APOE ...
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bioRxiv - Physiology 2024Quote: ... Tissue was then washed with 0.1 M phosphate/ citrate buffer and subsequently postfixed in 1% osmium tetroxide (19130, EMS) for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... immediately followed by cardiac perfusion with 10ml of 1% paraformaldehyde (PFA) prepared from 16% aqueous stock (Electron Microscopy Sciences). Tissues were then harvested and fixed for 24 hours in 1ml fixative buffer (BD Cytofix/Cytoperm prepared 1:4 buffered to PBS ...
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bioRxiv - Immunology 2024Quote: ... The final wash was into a final volume of 100μL of PBS + 1% (v/v) paraformaldehyde (Electron Microscopy Sciences). The plate with fixed samples was stored at 4°C in the dark wrapped in aluminum foil for no more than three days before analysis on the spectral flow cytometer.
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells in FACS buffer (PBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences) for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The grid was quickly washed using a 4-μL drop of milli-Q (MilliporeSigma) water and stained with 4 μL of negative stain solution composed of either 1% (w/v) uranyl acetate (EMS) with 0.5% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed in distilled water by centrifugation and resuspension and then post-fixed in 1% aqueous osmium tetroxide (Electron Microscopy Sciences) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2020Quote: ... The EV sample (5μl) was adsorbed for 1 min to a carbon-coated mesh grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-s exposure to a glow discharge (25 mA) ...
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bioRxiv - Neuroscience 2021Quote: Adult animals were perfused with 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA), brains ...
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bioRxiv - Neuroscience 2021Quote: After 8 washes with 0.1 M cacodylate buffer at pH 7.4 (SigmaAldrich) the tissue was post fixed by 1% osmium tetroxide (Electron Microscopy Sciences) and 0.6% K3Fe(CN)6 in a 0.1 M cacodylate buffer for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... ∼5 × 109 infected RBCs (iRBCs)] and schizont stage (40–46 hpi, ∼1.5 × 109 iRBCs) were harvested and crosslinked with paraformaldehyde (1% final concentration; EMS, USA) at 37°C for 15 min with agitation and then immediately neutralized by adding glycine (0.125 M final concentration ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed in water and then maleate buffer (MilliporeSigma) (pH=6.0) and stained in 0.05 M Maleate Buffer containing 1% uranyl acetate (Electron Microscopy Sciences) at 4°C overnight ...
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bioRxiv - Biophysics 2022Quote: ... A 3.5 μL sample containing the complex (1 mg/ml) was applied to a glow-discharged UltraAuFoil grid (R1.2/1.3, mesh 300) (Electron Microscopy Sciences), and then blotted and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2020Quote: Three microliters of the Ric-8A:ΔN31Gαi1:4Nb complex at 0.4 mg/ml with 0.01% NP40 were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (Electron Microscopy Sciences). Grids were blotted for 4 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were allowed to dry in open air for 1 hour prior to coverslipping with Cytoseal (Electron Microscopy Sciences #18007).
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bioRxiv - Neuroscience 2021Quote: ... Embryo buffer E3 was removed and replaced with 1 mL SHIELD Perfusion Solution with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences), shaking overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... for one hour and then washed three times for 15 min each with the same buffer before secondary fixation in 1% osmium tetroxide (EMS) prepared in Sorensen’s Phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... after which 5-μL were deposited in a glow-discharged Quantifoil grid (200-mesh copper R2/1, Electron Microscopy Sciences), followed by manual blotting and plunge freezing in a 50/50 ethane propane mix (Airgas ...
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bioRxiv - Microbiology 2020Quote: ... The mixture (2.5 μL) was applied to freshly glow-discharged (30 s) Quantifoil R 3.5/1 grids (Electron Microscopy Sciences) and manually blotted before being flash-frozen in liquid ethane ...
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bioRxiv - Cell Biology 2024Quote: ... pieces of tissue were rinsed with 0.1M sodium cacodylate buffer (cacodylate buffer) three times and then postfixed on ice with reduced osmium containing 1% osmium tetroxide (EMS) and 1% ferrocyanide (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Formvar carbon film on 200-mesh copper grids (FCF200-Cu) and uranyl formate (16984-59-1) from Electron Microscopy Sciences were used for sample preparation for negative-stain transmission electron microscopy (TEM).
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bioRxiv - Neuroscience 2024Quote: ... Subsequently, sections were incubated in DAPI (1:1000, 10 mins) and mounted with Fluoromount G (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Molecular Biology 2024Quote: ... and cover-slipped with ThermoFisher Gold Seal Cover Glass 24 x 60mm No.1 thickness (Electron Microscopy Sciences, 63770-01). Sections were imaged using a Leica CTR6000/B microscope to quantify sulfatide accumulation in different brain areas.
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bioRxiv - Biochemistry 2024Quote: The specimen was vitrified using 3 μL of freshly purified BasC-Nb71 complex in 0.17% DM applied to glow-discharged Quantifoil R 0.6/1 Cu 300 mesh grids (Electron Microscopy Sciences), at a concentration of 2.2 mg/ml ...
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bioRxiv - Genetics 2023Quote: AG437 pezo-1(R2405P) early L4 hermaphrodites were washed three times in M9 and soaked in 48 mM Ethyl methane sulfonate (EMS) solution for four hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... pelleted and washed with PBS before being resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences 30525-89-4) + 0.0075% glutaraldehyde (Sigma G6257 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then incubated in 0.1 M sodium cacodylate buffer supplied with 1% OsO4 and 0.8% potassium ferricyanide (Electron Microscopy Sciences), for 2 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Bioengineering 2023Quote: Purified DNA origami structures (1–10 nM) were adsorbed onto freshly glow discharged copper grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 4 minutes ...
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bioRxiv - Genetics 2023Quote: The two epididymides of mature males were recovered in 1 mL of 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) and the sperm were allowed to swim for 15 min at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... transcardial perfusion was performed using 1× phosphate-buffered saline (PBS) and 4% paraformaldehyde (PFA; Electron Microscopy Sciences, the United States). The mouse liver and kidney were dissected and postfixed in 4% PFA for 6 hours at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... an overlay of rat tail collagen type 1 solution (2.5 mg/mL) in NF medium (or EMS for disease conditions) was deposited on top of the hepatocytes inside the borders of the oval-well ...
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bioRxiv - Neuroscience 2024Quote: ... 500ul 1% neutral red dye in PBS was injected intraperitoneally 2 hours prior to cardiac perfusion with ice cold fixation buffer (2.5% glutaraldehyde, 1% paraformaldehyde, in 0.2 M sodium cacodylate buffer (Electron Microscopy Sciences). Lesion tissues were then dissected out and cut into 2mmx2mm sections ...
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bioRxiv - Immunology 2024Quote: Neutrophils migrating under the agarose for 1 h in 8 well chamber dishes were fixed using 4% paraformaldehyde (PFA, EMS #) in PHEM buffer for 20 min at 37 °C followed by agarose removal from the wells ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of culture was sampled (Fig. S1) and chemically fixed by adding paraformaldehyde (PFA; Electron Microscopy Science; EM grade) to a final concentration of 2% ...
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bioRxiv - Molecular Biology 2024Quote: Enucleated adult eyes were fixed for 1 hour at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science, Hatfield, PA) prepared in phosphate-buffered saline (1X PBS ...
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bioRxiv - Biophysics 2024Quote: ... 3 µL of each sample (∼1 mg/mL) was applied to a freshly glow discharged (15mA, 1 min) holey carbon copper grid (apo-37C: Cflat (EMS) R1.2/1.3 ...
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bioRxiv - Biophysics 2024Quote: ... 2.5% glycerol) was applied to glow discharged gold grids (Au-flat, 0.6 µm/1 µm, 300 mesh, Electron Microscopy Sciences). The sample was blotted with the minimum blot time that can be set (0 s) ...
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bioRxiv - Biochemistry 2024Quote: ... AFM samples were prepared by adsorbing 10 μl of 5 times diluted sample onto mica (Muscovite mica, V 1 quality, EMS) for 45 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... mice were perfused transcardially with heparinized saline (10 units mL−1 of heparin) and 4% paraformaldehyde (PFA) that was freshly prepared from 32% PFA aqueous solution (Cat# 15 714, EMS), using a peristaltic pump at a rate of 7 mL min−1 ...
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bioRxiv - Cell Biology 2024Quote: ... Sapphire discs with attached frozen hTERT-RPE-1 cells and type-A planchettes containing worms were transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS) and 0.1% (w/v ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The slices were then transferred to capped glass vials and postfixed with 1% osmium tetroxide diluted in PBS from a 4% aqueous solution (EMS 19170) for 30 minutes on a rotator ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Biochemistry 2020Quote: ... 4μL of the resulting solution was deposited on glow-discharged Quantifoil grids (R2/1, Cu 200-mesh grid, Electron Microscopy Sciences). The solution on the grid was then blotted using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µL of resuspended cells were pipetted onto agarose pads in 13 x 1 mm silicone isolator wells (Electron Microscopy Sciences) and covered with a No ...
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bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...