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Citations for Electron Microscopy Sciences :
101 - 137 of 137 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Grids were glow discharged before for 30 seconds at 25 mA using a GloQube® Plus Glow Discharge System (Electron Microscopy Sciences). The sample was blotted off ...
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bioRxiv - Neuroscience 2023Quote: ... Two rounds of 100% acetonitrile (30 minutes each) served as a transitional solvent step before proceeding to epoxy resin (EMS Hard Plus). A progressive resin infiltration series (1:2 resin:acetonitrile (e.g ...
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Neuroscience 2023Quote: ... At −50 °C samples were washed four times in ethanol for 30 minutes each before gradual infiltration with HM20 resin (Electron Microscopy Sciences). Polymerization of the resin was done with a UV light source and was carried out at −50 °C for 48 hours followed by post-polymerization at room temperature for 2 days.
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bioRxiv - Immunology 2023Quote: ... The 3.5 µl of sample mixture was put onto a glow discharged carbon coated Cu grids for 30 secs (EM grid, 300 mesh, Electron Microscopy Sciences). After 1.5 min of incubation of the sample on the grid ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Neuroscience 2024Quote: ... Two rounds of 100% acetonitrile (30 minutes each) served as a transitional solvent step before proceeding to epoxy resin (EMS Hard Plus). A progressive resin infiltration series (1:2 resin:acetonitrile (e.g ...
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bioRxiv - Neuroscience 2023Quote: ... Two rounds of 100% acetonitrile (30 minutes each) served as a transitional solvent step before proceeding to epoxy resin (EMS Hard Plus). A progressive resin infiltration series (1:2 resin:acetonitrile (e.g ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were incubated with 200nM Mitotracker for 30 min before fixing them in 4% paraformaldehyde (Electron Microscopy Sciences Cat# 15710, Hatfield, PA) for 15 min ...
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bioRxiv - Biophysics 2022Quote: ... The 3.5 μl of sample mixture were applied onto freshly glow discharged carbon coated Cu grids (30 secs) (EM grid, 300 mesh, Electron Microscopy Sciences). After 1 min ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...
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bioRxiv - Immunology 2024Quote: ... At -60 °C samples were infiltrated with increasing concentrations (30, 60, and 100% for 1 h each) of Lowicryl (3:1 K11M/HM20 mixture; Electron Microscopy Sciences). After 5 h of 100% Lowicryl infiltration ...
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bioRxiv - Neuroscience 2024Quote: ... fluorescently tagged goat anti-mouse and anti-chicken secondary antibodies were diluted in PBS and incubated for 30 min and coverslips were mounted using Fluoromount-G mounting medium (Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were then counterstained with Hoechst 33342 (1:10,000) and mounted using Fluoro-gel in TES Buffer (Electron Microscopy Sciences, 17985-30). The slides were imaged using a Zeiss Observer D1 microscope with a 40x objective.
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bioRxiv - Biochemistry 2024Quote: ... mesh 300 grids with 2 nm amorphous carbon layer on top were glow discharged for 30 s at 15 mA (EMS-100 Glow Discharge System, Electron Microscopy Sciences). 3 µL of a 140 nM mitoribosome sample was applied on the grid and incubated for 30 s at 100% humidity and 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 3 mL of cells was harvested by centrifugation for 3 min at 1500 × g at room temperature and fixed for 30 min in 1 mL of 2.5% (v/v) glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) in 0.1 M cacodylate buffer (Electron Microscopy Sciences ...
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bioRxiv - Microbiology 2020Quote: Carbon-coated copper grids were glow discharged for 30 seconds at 25 mA using a GloQube® Plus Glow Discharge System (Electron Microscopy Sciences). Four microlitres of diluted sample was applied to the grids and incubated for 30 seconds ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in HBSS(-) for 30 min at room temperature (RT) and then immediately fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: Cells plated on glass coverslips were processed for chemical fixation (CF) by incubation for 30 minutes at room temperature with 0.2% glutaraldehyde (Electron Microscopy Science, Cat.16220) and 2.5% paraformaldehyde (PFA ...
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bioRxiv - Immunology 2022Quote: ... they were perfused with 40 mL of ice-cold PBS(-) and then with 30 mL of ice-cold 4% PFA (Electron Microscopy Science 15714) in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... dehydrated in a graded series of ethanol ranging from 30% to absolute solution and embedded in resin (EMBed-812, Electron Microscopy Sciences, USA). 80 nm thick sections from at least four blocks from Ctr iPSC-derived MYOrganoids in presence or absence of Ctr fibroblasts were stained with uranyl acetate and lead citrate ...
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bioRxiv - Neuroscience 2022Quote: ... specimens were dehydrated using serially graded ethanol solutions (30%, 50%, 70%, 90% and 100%) and infiltrated and embedded in Spurs resin (Electron Microscopy Sciences, Hatfield, PA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... A 30 µL drop of each sample was placed under a 100-mesh formvar-carbon coated grid FCF100-Cu-UB (Electron Microscopy Sciences, Hatfield, PA) with 1% uranyl acetate ...
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bioRxiv - Cancer Biology 2021Quote: ... then gently dipped four times into molecular biology-grade water before inversion onto one drop of Fluoro- Gel (with TES Buffer, Electron Microscopy Sciences, #17985-30) then allowed to air-dry in the dark for at least 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: Gels were fixed by 30 minutes rotation incubation at room temperature in freshly prepared 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA, Cat# 15710S) diluted in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... were injected intraperitoneally in n=3 control and n=3 KA Nestin-GFP animals 30 minutes before perfusion with a PFA 4 %-Glutaraldehyde 0.5 % (Cat#16536-05, Electron Microscopy Sciences, Hatfield, PA, USA) mixture ...
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bioRxiv - Bioengineering 2024Quote: ... and EV-CLIPs) was vortexed for 30 s and drop-casted onto a Formvar/Carbon 300 Mesh TEM grid (Electron Microscopy Sciences, FCF300-CU). It was dried overnight inside a Petri dish at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: Carbon-coated copper grids were glow-discharged for 30 s at 25 mA using a GloQube Plus Glow Discharge System (Electron Microscopy Sciences, PA, USA). Grids were coated with 4 μl protein ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were fixed in 2.5% glutaraldehyde plus 1% paraformaldehyde in 0.1 M cacodylic buffer for 30 min and then embedded in resin using an Embed 812 kit (Electron Microscopy Sciences, Hatfield, PA, USA) and sectioned at 80 nm with a diamond knife (Diatome ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were stained with DAPI at RT for 30 s and sections were mounted with Fluoro-Gel II with DAPI (Electron Microscopy Sciences, Lot#220909) and covered with coverslips.
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bioRxiv - Pathology 2020Quote: ... glutaraldehyde for 1 h at room temperature then treated with 1% osmium tetroxide and dehydrated through a graded series of ethanol concentrations (from 30 to 100%) and embedding with an Embed 812 kit (Electron Microscopy Sciences, Fort Washington, PA). Ultrathin sections (60 to 80 nm ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Neuroscience 2022Quote: ... Excess PBS was removed and slices were left to dry for 30 min and subsequently covered with Fluoro Gel with DABCO (Electron Microscopy Sciences, Cat no. 17985) and a rectangular coverslip.
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bioRxiv - Developmental Biology 2024Quote: ... before progressive dehydration in an ethanol series (30-50-70-80-90- 100%, at least 20 minutes per step) and chemical drying with hexamethyldisilazane (HMDS; Electron Microscopy Sciences 50-243-18). Dry samples were mounted on aluminum stubs using double-sided carbon tape and sputter-coated with gold-palladium before imaging on a Hitachi 3000N VP electronic microscope at 20kV.
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bioRxiv - Cell Biology 2021Quote: Cells were cultured on cell-tight C-2 cell disks (Sumitomo Bakelite, Tokyo, Japan; #MS-0113K) and fixed for 2 h on ice in 2.5% glutaraldehyde (Electron Microscopy Science, Hatfield, PA; #111-30-8) in 0.1 M phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: Samples described above were diluted in Buffer C and deposited onto freshly glow-discharged (EMS-100 Glow Discharge System, Electron Microscopy Sciences, 30 s at 15 mA) copper Quantifoil (Quantifoil Micro Tools GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... Ducupan Resin A/M, cat. no. 44611; Sigma-Aldrich, B hardener, cat. no. 44612; Sigma-Aldrich, D hardener cat. no. 44614; Electron Microscopy Sciences, DMP 30 cat. no. 13600)