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Citations for Electron Microscopy Sciences :
51 - 100 of 137 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were dehydrated in an ethanol series (30, 50, 75, 95% and 100%, Electron Microscopy Sciences, #15056) at 4°C followed by three additional changes of absolute ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... Dehydration in graded series of ethanol (30, 50, and 70%) was followed by 2% uranyl acetate (EMS) incubation for 2.5 h ...
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bioRxiv - Molecular Biology 2023Quote: ... heat-indued antigen retrieval was performed for 30 min in Buffer A (Electron Microscopy Sciences, pH 6). Tissue sections were blocked using 0.3% H2O2 ...
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bioRxiv - Plant Biology 2022Quote: ... 75% and 100% Quetol resin (hard formulation) in propylene oxide without DMP-30 (#14640; EMS, Hatfield, PA, USA) at room temperature for 24 hours each step on a rocking platform to enhance resin infiltration ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were mounted on glass-microscope slides using Fluoro-Gel Mounting Medium (Electron Microscopy Sciences, 17985-30). Representative pictures were taken with Zeiss microscope equipped with Colibri 7 LED and Axiocam 705 camera ...
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bioRxiv - Microbiology 2023Quote: HFFs were infected for 30 h and fixed with 2.5% glutaraldehyde (EM grade, Electron Microscopy Sciences, Hatfield, PA) in 0.1 M sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: Spheroids (in glass-bottom dishes) were fixed for 30 min in 3.7% paraformaldehyde (PFA, #15714, Electron Microscopy Sciences) in PHEM buffer (60mM PIPES ...
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bioRxiv - Cell Biology 2024Quote: ... and they were fixed at 4°C for 30 minutes with 4% PFA (Electron Microscopy Sciences; Cat #15713S). For immunofluorescence of 5-Methylcytosine (5mC) ...
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bioRxiv - Neuroscience 2021Quote: ... When birds were unresponsive to a toe-pinch they were transcardially perfused with 20 ml 0.1M PBS (pH=7.4) followed by 30 ml 4% paraformaldehyde (PFA, pH=7.4; Electron Microscopy Sciences). Brains were carefully dissected ...
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bioRxiv - Neuroscience 2021Quote: ... followed by adding ddH2O up to a final volume of 30 mL after accounting for glutaraldehyde (Electron Microscopy Science) volume for final BSA concentrations equal to ...
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bioRxiv - Biochemistry 2021Quote: ... 30 sec in air at 20 mA and 0.4 mbar) C-flat 1.2/1.3-3Au grids (Electron Microscopy Sciences), and blotted for 4 sec using a Vitrobot Mark IV (FEI ...
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bioRxiv - Developmental Biology 2020Quote: Organoid cultures were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Biophysics 2020Quote: ... 400 Cu mesh grids were glow discharged for 30 sec at 25 mA with a K100X glow discharger (EMS), 4 μl of sample was added to the grid left for 1 min ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated in cacodylate buffer containing 1% low molecular weight tannic acid for 30 min (Electron Microscopy Sciences) between the osmication step and the uranyl acetate step ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then treated with propylene oxide, impregnated in resin (Embed 812, Araldite 502, DDSA, DMP-30 (all EMS)) overnight at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Organoids were cryopreserved in 50:50 solution of 30% sucrose/PBS: tissue freezing medium (Electron Microscopy Sciences, Hatfield, PA) and cryosectioned (15 μm) ...
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bioRxiv - Bioengineering 2024Quote: ... and pre-charged at negative glo for 30 seconds using a GloQube Plus Glow Discharge system (Electron Microscopy Sciences). These charged grids were incubated with exosome samples for 2 minutes followed by three washes ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then treated with propylene oxide, impregnated in resin (Embed 812, Araldite 502, DDSA, DMP-30 (all EMS)) overnight at RT ...
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bioRxiv - Bioengineering 2024Quote: ... Organoids were cryopreserved in 50:50 solution of 30% sucrose/PBS: tissue freezing medium (Electron Microscopy Sciences, Hatfield, PA) and cryosectioned (15 μm) ...
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bioRxiv - Bioengineering 2024Quote: ... cells were fixed for 30 minutes at room temperature with 4% paraformaldehyde (Electron Microscopy Science, Hatfield, PA, USA; 15700) in PBS and then permeabilized with 90% methanol for 10 minutes at –30°C ...
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bioRxiv - Microbiology 2024Quote: ... The cultures were grown for 3 hours with vigorous shaking every 30 minutes (to keep the EMS in solution). The cultures were passed out of the chamber and centrifuged at 3,000 x g for 10 minutes ...
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bioRxiv - Plant Biology 2024Quote: A glow discharged (30 s, 25 mA) Quantifoil 2/1 Au 300 mesh electron microscopy grid (Electron Microscopy Sciences) was mounted in a Thermo Fisher Vitrobot Mark IV system set to 100% humidity and 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and coverslips were mounted on glass microscope slides using Fluoro-Gel Mounting Medium (Electron Microscopy Sciences, 17 985-30). Images were taken with Nikon A1R HD scanning confocal microscope using a 40x objective and analyzed using Imaris 10.0 software (Oxford Instruments) ...
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bioRxiv - Biochemistry 2020Quote: 300-mesh copper grids with a continuous carbon film (EM Resolutions, C300Cu100) were glow-discharged for 30 s at 45 mA with a 100x glow discharger (EMS). 4-µl samples were applied to glow-discharged grids and incubated for 1 minute ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then placed in two serial washes of PBS solution with gentle agitation for 30 seconds each prior to being submerged for 2 hours in 2% osmium solution (RT 19172, EMS). Thereafter ...
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bioRxiv - Neuroscience 2020Quote: ... 75-90nm sections from this block were mounted on Formvar-carbon coated slot grids and contrast stained for 30 seconds in 3.5% uranyl acetate in 50% acetone (EMS, 10015), followed by 0.2% lead citrate (EMS ...
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bioRxiv - Microbiology 2020Quote: ... The mixture (2.5 μL) was applied to freshly glow-discharged (30 s) Quantifoil R 3.5/1 grids (Electron Microscopy Sciences) and manually blotted before being flash-frozen in liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: Brains were sectioned (30 μm) using a freezing microtome and sections were mounted on Superfrost Plus slides (Electron Microscopy Sciences). Sections were quenched with 0.1 M PB (pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
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bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
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bioRxiv - Genetics 2023Quote: ... immediately followed by fixation at room temperature for 30 min using a 4% paraformaldehyde solution (Electron Microscopy Sciences, (PN:15714)) in PBS ...
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bioRxiv - Physiology 2024Quote: ... slides were heated for 30 minutes at 65°C followed by submersion in Histoclear II (Electron Microscopy Sciences 64111-04) twice for five minutes ...
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bioRxiv - Biochemistry 2024Quote: ... mesh 300 grids with 2 nm amorphous carbon layer on top were glow discharged for 30 s at 15 mA (EMS-100 Glow Discharge System ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μl of diluted sample was deposited onto a glow-discharged (Edwards S150B, 30sec, 30-50 mA, 1,2kV, 10-2 mbar) continuous carbon grid (CF400-CU-UL, Electron Microscopy Sciences). After one minute at RT ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of sample was applied to glow-discharged (30 s) Quantifoil copper grids (R2/2, 200 mesh, Electron Microscopy Sciences), blotted on both sides for 4 s ...
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bioRxiv - Cell Biology 2021Quote: ... cells were gently washed with 30 μL of PBS and fixed with 1% (v/v) paraformaldehyde (Electron Microscopy Sciences 15710-S) diluted in PBS at room temperature for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of purified proteins or immune complexes (∼0.02 mg/mL) were applied onto glow discharged (20 mA for 30s) carbon-coated 400 mesh copper grids (Electron Microscopy Sciences). After 5 s ...
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bioRxiv - Cell Biology 2024Quote: ... or 30 min and fixed by adding an equal volume of 4 % pre-warmed paraformaldehyde (Electron Microscopy Sciences, final concentration 2 %), followed by 20 min incubation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were rinsed three times at five-minute intervals with deionized ultrapure water followed by a 30-minute incubation at RT with a filtered (Whatman, 0.2 μm) freshly prepared solution of 1% thiocarbohydrazide (Electron Microscopy Sciences) made by dissolving it at 60°C for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... Sections were fixed in 10% formalin for 30 minutes at room temperature and incubated in Bouin’s fixative solution (Electron Microscopy Sciences) overnight at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... antennae were washed twice in 3% Triton-PBS and twice in 0.1% Triton-PBS and mounted in Vectashield (Vectorlabs) on 30-well PTFE printed slides (Electron Microscopy Sciences) before imaging on a Zeiss LSM710 confocal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then transferred into an ice filled bath and washed 4× for 30 min each with acetone and infiltrated with Spurr’s resin (Electron Microscopy Sciences). During final steps of resin infiltration ...
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bioRxiv - Cell Biology 2024Quote: ... En bloc staining was performed with 1% (aq) uranyl acetate for 30 min Samples were embedded into BEEM® capsules filled with EPOXI resin (Epon 812 from EMS) and baked for 48 hours at 68-70°C ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Genomics 2022Quote: ... biological specimens were transferred to microporous specimen capsules (30 μm porosity, 12 mm diameter, 11 mm high, ref #70187-20, Electron Microscopy Science) and dehydrated in a graded series of ethanol in double-distilled water (50 ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed for 10 or 30 (if infected with SARS-CoV-2) min in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2022Quote: ... The samples were dehydrated in graded concentrations of ethanol (30%, 50%, 70%, 90%, 95%, and 100%) and embedded in Epon resin (Electron Microscopy Sciences). Ultrathin sections (80 nm ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...