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Citations for Electron Microscopy Sciences :
1051 - 1098 of 1098 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 3 ul chromatin samples with a concentration of about 0.2 mg/ml DNA were applied to Quantifoil R2/2 200 mesh copper grids (EMS Q250-CR2). Vitrification was conducted by plunging into liquid ethane using our FEI Vitrobot Mk IV Grid Plunging System at 100% humidity ...
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bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µl of DELE1 samples at 0.1 mg/ml were applied onto carbon coated 400 A° mesh Cu-Rh maxtaform grids (Electron Microscopy Sciences) which were plasma cleaned for 15 s using Pelco glow discharge cleaning system (Ted Pella ...
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bioRxiv - Cancer Biology 2024Quote: ... and pellets were resuspended in a 5 ml volume of fixative master mix of Paraformaldehyde-Glutaraldehyde Solution Karnovsky’s Fixative (Electron Microscopy Sciences). Fixative solution was prepared with 16% paraformaldehyde solution ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 mg/mL) were incubated for 60 seconds on the carbon face of 200 mesh copper grids (Electron Microscopy Sciences) following their glow discharge at 25 mA for 30 seconds ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of purified proteins or immune complexes (∼0.02 mg/mL) were applied onto glow discharged (20 mA for 30s) carbon-coated 400 mesh copper grids (Electron Microscopy Sciences). After 5 s ...
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bioRxiv - Biophysics 2023Quote: ... one quarter of the bell from each medusae was chemically fixed in 10 mL of 2.5% [v/v] glutaraldehyde (Electron Microscopy Sciences), 4% [v/v] paraformaldehyde (Electron Microscopy Sciences) ...
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bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Biochemistry 2023Quote: ... a 4 μl aliquot of the sample (diluted concentration: 0.25 mg/ml) was placed on ultra-thin carbon film grids (CF-200-Cu-UL, Electron Microscopy Sciences, Hatfield ...
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bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Animals were deeply anesthetized with ketamine/xylazine and transcardially perfused with 5-10 mls of 37°C 0.9% sterile saline followed by 10 mls of room temperature 4% EM Grade paraformaldehyde (PFA, Electron Microscopy Sciences) in 0.9% saline.
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bioRxiv - Plant Biology 2024Quote: ... The proteins were diluted to 0.01 mg/mL and 4 μL was applied to a glow discharged Formvar/Carbon grid (Electron Microscopy Sciences) for 30 s ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of spike-protein at 0.03 mg/ml in 1X TBS was deposited on a glow-discharged carbon coated copper mesh grid (Electron Microscopy Sciences) and blotted off ...
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bioRxiv - Bioengineering 2024Quote: ... A small drop (2.5 mL) of the protein solution was applied to the carbon side of a glow-discharged grid (EMS, CF300-CU) and left on the grid for 1 min ...
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bioRxiv - Molecular Biology 2020Quote: 3 µL of NT-NTR1-Gαi1β1γ1-cND complex at a concentration of 0.02 mg/mL was applied onto a glow-discharged continuous carbon grid (Electron Microscopy Sciences, Inc.). After two minutes of adsorption ...
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bioRxiv - Immunology 2019Quote: ... Eight to 10 ml of blood was gently fixed and lysed by incubating with 792 µl of 16% paraformaldehyde (Electron Microscopy Sciences, Hatfield ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Neuroscience 2021Quote: ... The animals were intracardially perfused with 100 mL of 0.9% physiological saline followed by approximately 250 mL of freshly prepared 0.1 M sodium cacodylate buffer (pH 7.4; Electron Microscopy Sciences cat #11653), which contained 2.5% glutaraldehyde (Electron Microscopy Sciences cat #16320 ...
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bioRxiv - Microbiology 2020Quote: Complexes of M4H2K1 Fab and BG505 UFO.664 trimer were purified by SEC to remove unbound Fab and diluted to 0.01 mg/mL in Tris-buffered saline prior to adsorption onto carbon-coated and plasma cleaned copper mesh grids (Cu400, Electron Microscopy Sciences). Grids were stained with 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were incubated at a molar ratio of 4 Fab:3 spike monomer for ∼1 h and approximately 3 µL of the sample at concentrations of about 10 to 15 µg/mL was applied to a glow discharged grid with continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences). The grids were stained with 0.75% uranyl formate (UF ...
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bioRxiv - Immunology 2020Quote: ... 10 mM EDTA) to a final concentration of ∼50-200 µg/ml and immediately applied to the glow-discharged grid (Electron Microscopy Sciences). After 30 seconds ...
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bioRxiv - Neuroscience 2021Quote: ... to be non-responsive to toe pinch and transcardially perfused with 10 ml of 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences (EMS)) to flush the vasculature followed by buffered fixative made of 2.5% glutaraldehyde (EMS) ...
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bioRxiv - Neuroscience 2023Quote: ... all animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Biophysics 2024Quote: 3 µL diluted MgtA (0.01 mg/mL) was deposited on a carbon-coated 400 mesh copper grid (CF400-CU, EMS, Hatfield, PA, USA) following glow discharging (PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Neuroscience 2024Quote: ... mice were perfused with phosphate-buffered saline (PBS, warmed up to 37 °C) with heparin solution (20 units / mL) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: 5 μl of 0.02 mg/ml purified Nsp15 sample was applied onto glow-discharged ultrathin formvar/carbon supported copper 400-mesh grids (Electron Microscopy Sciences), blotted and stained with 2.0% uranyl acetate ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with pentobarbital (Fatal-Plus, Vortech Pharmaceuticals) and intracardially perfused with ∼50 ml of 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the appropriate plane of anesthesia was checked by a lack of toe pinch reflex and the animals were transcardially perfused with 15 ml 0.15 M cacodylate buffer (EMS, Hatfield, PA, pH 7.4) followed by 30 ml fixative mixture containing 0.08 M cacodylate (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 20,000 seeds homozygous for the SCRpro::GFP-SCR transgene were treated overnight at room temperature with 10 mL 0.1% ethyl methanesulfonate (EMS, Sigma M0880, MO, USA). After thorough washing ...
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bioRxiv - Neuroscience 2021Quote: ... the appropriate plane of anesthesia was checked by a lack of toe pinch reflex and the animals were transcardially perfused with 15 ml 0.15 M cacodylate buffer (EMS, Hatfield, PA, pH 7.4) followed by 30 ml fixative mixture containing 0.08 M cacodylate (pH 7.4) ...
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bioRxiv - Neuroscience 2021Quote: 275 μm slices containing dopamine neurons filled with neurobiotin-containing internal solution (4 mg/ml) during patch-clamp experiments were fixed in 4% paraformaldehyde solution (Electron Microscopy Sciences: 15713) in 1x PBS for 24-48 h at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
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bioRxiv - Neuroscience 2022Quote: ... Fibril assemblies were next analyzed via transmission electron microscopy at an applied concentration of 0.4 mg per mL, with fibrils absorbed to glow-discharged 300 mesh (Ladd Research Industries, Inc.) and stained with 2% uranyl acetate (Electron Microscopy Sciences, Inc.) in water ...
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bioRxiv - Plant Biology 2022Quote: ... without mPrep screens and held in mPrep CPD Holder (Microscopy Innovations, Cat # 2250) that was then placed in a 50 ml PTFE beaker (EMS Diasum, Cat# 60942) containing LN2 frozen substitution fluid (2% osmium tetroxide in acetone with 3% water ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Neuroscience 2024Quote: 275 μm slices containing Grpr-eGFP+/− NAc MSh neurons were filled with neurobiotin-containing internal solution (4 mg/mL) during patch-clamp experiments and then fixed in 4% paraformaldehyde solution (Electron Microscopy Sciences: 15713) in 1X PBS for 24–48 hr at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... NaNO2 (# S25560) and NaCl (# 7647-14-5) which is followed by perfusion with 40 mL of cold 4% paraformaldehyde (Electron Microscopy Sciences, #RT-15714) in 1X-phosphate buffered saline (PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μl of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5mM Mg(OAc)2 wash buffer to an optical density of 2 A260/mL (corresponding to 40 nM of 80S) and deposited on copper grids (Electron Microscopy Sciences, CF400-CU). Grids were glow-discharged at 15 mA for 30s 4 uL of sample were incubated on the glow-discharged grids for 1min ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and then perfused transcardially with room temperature (RT) phosphate buffered saline (PBS) with heparin (0.02 mg/mL) and then ice-cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, catalog no: 15714). Brains were dissected and post-fixed in 4% PFA overnight at 4°C and cryopreserved in 30% sucrose in PBS for ∼2 days at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Then the cells were counted and diluted to 106 cells/ml and fixed with 4% paraformaldehyde aqueous solution (Electron Microscopy Sciences #15710, Formaldehyde methanol-free) for 10 minutes at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of high titre phage stock (~109 PFU/mL) was loaded onto plasma-glowed carbon coated 300 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA, USA). After 60 s the excess specimen was removed by blotting and 10 μL of 1% w/v phosphotungstic acid (PTA ...
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bioRxiv - Bioengineering 2020Quote: Animals were implanted with a stainless-steel probe or a Fiber F1/F2 for 4 weeks and then anesthetized with a ketamine (20 mg/mL)/xylazine (2 mg/mL) solution and transcardially perfused with phosphate-buffered saline (PBS) (Fisher BP661-10) followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Cat. #15714-S) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...