Labshake search
Citations for Electron Microscopy Sciences :
751 - 800 of 1023 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
-
bioRxiv - Cell Biology 2022Quote: ... the flow chamber was washed with a second reaction mix containing 25 µM tubulin before fixing with 1% glutaraldehyde (Electron Microscopy Sciences) for 5 minutes at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grid covered with a formvar film (FF2010-Cu, Electron Microscopy Sciences, USA). Sections were covered by 0.1 μm TetraSpeck microsphere fiducials (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2022Quote: ... 4 mm diameter PDMS wells were passively adhered to #1 9 mm x 9 mm glass coverslips (Electron Microscopy Sciences #72190-09). Wells were passivated overnight using vapor deposition of Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma 448931 ...
-
bioRxiv - Cell Biology 2019Quote: For negative staining 3 µl of purified 40S+60S ribosome fractions or alternatively 3 µl of 40S+60S ribosome fractions in the presence of (PR)20 (1:10 molar ratio) were deposited onto freshly glow-discharged carbon-coated 400 mesh copper electron microscopy (EM) grids (Electron Microscopy Sciences) and retained on the grid for 1 min ...
-
bioRxiv - Physiology 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples of the complex (2 μL) were applied to glow-discharged (1 min, easiGlow, Pelco) R2/2 300-mesh Quantifoil holey carbon grids (Electron Microscopy Sciences). The grids were blotted for 2 s with Whatman 41 filter paper and flash-frozen by plunging into liquid ethane using a Leica EM CPC manual plunger ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu, Electron Microscopy Sciences) followed by plunging into liquid ethane using an FEI Vitrobot Mark IV set to a blot time of 22 and a blot force of 22 for QYNNENNFV and 24 for GSTVYAPFT ...
-
bioRxiv - Biochemistry 2021Quote: ... 1.8 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV using a blot time of 25-30 seconds and a blot force of 22 ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...
-
bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
-
bioRxiv - Neuroscience 2021Quote: ... was applied to glow-discharged Quantifoil holey carbon grids (Gold R2/1; 200 mesh) overlaid with an additional 2-nm carbon layer (Electron Microscopy Sciences). The grids were blotted for 3-5 s and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Genomics 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
-
bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
-
bioRxiv - Neuroscience 2023Quote: ... Coverslips were then counterstained with Hoechst 33342 (1:10,000) and mounted using Fluoro-gel in TES Buffer (Electron Microscopy Sciences, 17985-30). The slides were imaged using a Zeiss Observer D1 microscope with a 40x objective.
-
bioRxiv - Physiology 2022Quote: ... freshly isolated pituitary glands were fixed with 2.5% formaldehyde/glutaraldehyde in 0.1 M Sodium Cacodylate Buffer (pH 7.4, Emsdiasum) and 1% OsO4 (Electron Microscopy Science, Cat No. 15949), followed by dehydration and staining with uranyl acetate/lead citrate ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
-
bioRxiv - Neuroscience 2022Quote: ... Grids were washed with two drops of double-distilled H2O and stained with two drops of freshly prepared 1% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were analysed at room temperature using a Tecnai Spirit (FEI ...
-
bioRxiv - Microbiology 2022Quote: ... Washed tardigrades were dried onto one field of a 76 × 26 × 1 mm 10-well (6mm) polytetrafluoroethylene-printed slide (Electron Microscopy Sciences). Fixation of specimens was performed by adding 30 μL of ice-cold 3:1 4% paraformaldehyde:1x phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: Organoids were dissociated and 75,000 cells were replated overnight in 2D on 12 mm round #1 coverglass (Electron Microscopy Sciences 72231-01) coated with 75 μg/ml Poly-D-lysine followed by 3% Matrigel ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were then washed in cytoskeletal buffer and fixed for 1 hour at room temperature in 4% formaldehyde (Cat# 1570, Electron Microscopy Sciences) in PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... In-house graphene grids were prepared from Trivial Transfer Graphene sheets (ACS Material) and QUANTIFOIL R2/1 mesh 300 gold (Electron Microscopy Sciences) by following a protocol introduced by Ahn et. ...
-
bioRxiv - Biochemistry 2023Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
-
bioRxiv - Microbiology 2023Quote: ... 3 μl of this mixture was blotted on a glow-discharged Quantifoil R 2/1 Copper 300 mesh grid (Electron Microscopy Sciences) and plunge-frozen in liquid ethane using a Vitrobot plunge freezer (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... iPS cell-derived neuronal cultures were fixed using 4% paraformaldehyde diluted in 1 PBS from a 16% aqueous solution (Electron Microscopy Sciences) for 20 min and then permeabilized with 0.2% Triton X-100 (Sigma) ...
-
bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
-
bioRxiv - Biophysics 2024Quote: ... These cells in the log-growth phase were collected and deposited on glow-discharged Quantifoil grids (R 2/1, Cu 200-mesh grid, Electron Microscopy Sciences), as described previously (5) ...
-
bioRxiv - Cell Biology 2024Quote: ... were immersed in and fixed by 4 % glutaraldehyde (48 h/4 °C), post-fixed by 1% osmium tetroxide (2 h, RT) and were embedded into Epon resin (Electron Microscopy Sciences) as described previously32 ...
-
bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by 20 ml of fixative containing 2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by 20 ml of fixative containing 2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by 40 ml 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were removed from the cranium and placed in 4% PFA for 48 hours at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... supplemented with 0.48 mg/ml ECL07 Fab and 0.085 mM n-dodecyl β-D-maltoside was applied to glow-discharged 400-mesh R1.2/1.3 C-flat holey carbon grids (Electron Microscopy Sciences; product code CF413-50-Au) for 1 min ...
-
bioRxiv - Plant Biology 2019Quote: ... using fine forceps and a 27-gauge needle as a scalpel, developing seeds were dissected from siliques and immediately immerged in fix solution (1×PBS, 4% formaldehyde (Electron Microscopy Sciences, 15713), and 0.4% dimethyl sulfoxide ...
-
bioRxiv - Microbiology 2019Quote: Equal numbers of bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29 ...
-
bioRxiv - Neuroscience 2021Quote: Coronal sections (n=2 mice, P30) and whole mounts (n=2 mice, P28) were incubated with Immunosaver (1:200; EMS, Fort Washington, PA) for 20 min at 60°C ...
-
bioRxiv - Microbiology 2020Quote: ... Then cells were put in propylene oxide for 1 h and infiltrated overnight in a 11 mixture of propylene oxide and Spurr’s low viscosity resin (Electron Microscopy sciences, Hatfield, PA). The cells were embedded in Spurr’s resin and polymerized at 60°C for 48 h ...
-
bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
-
bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
-
bioRxiv - Bioengineering 2020Quote: ... kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI) made of 1% OsO4 (Electron Microscopy Sciences, 19190), 1.25% potassium hexacyanoferrate (II ...
-
bioRxiv - Neuroscience 2021Quote: ... Neurobasal media was carefully aspirated from all wells and coverslips were briefly rinsed with ice cold 1× PBS with Ca2+/Mg2+ followed by fixation in freshly made 4% PFA (Electron Microscopy Sciences: 15713) in 1x PBS for 10 min ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...
-
bioRxiv - Microbiology 2020Quote: ... Bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (46) ...
-
bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.