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Citations for Electron Microscopy Sciences :
1001 - 1050 of 7095 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature with 4% PFA (Electron Microscopy Sciences) diluted in PBS and permeabilized with 70% ethanol overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and the sphingolipid-deficient (MVKCg9) strains were mixed with 6 nm BSA gold tracers (Electron Microscopy Sciences) to facilitate tilt series alignment ...
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bioRxiv - Cell Biology 2024Quote: ... infiltrated with and embedded in EMbed812 epoxy resin (#14120, Electron Microscopy Sciences) and then sectioned at 100 nm thickness with a Leica EM UC7 ultramicrotome ...
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bioRxiv - Synthetic Biology 2024Quote: ... and placed onto formvar/carbon nickel slot grids (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Plant Biology 2024Quote: ... copper or nickel grids formvar-coated grids (Electron Microscopy Sciences, # FCF300) were prepared using glow discharge at 0.4 mBar and 15 mA for 1 min (PELCO easiGlow #91000 and #91040) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were fixed directly by adding paraformaldehyde (PFA) (Electron Microscopy Sciences, 15714S) to the medium (final concentration 4% ...
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bioRxiv - Genetics 2024Quote: ... Cu grids (Electron Microscopy Sciences, FCF300-Cu-50) were glow discharged using 15 mA for 45 s ...
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bioRxiv - Neuroscience 2024Quote: ... followed by heat induced antigen retrieval in a programmable antigen retrieval cooker (Aptum Bio Re-triever 2100) using 1X R-UNIVERSAL Epitope Recovery Buffer (EMS, #62719-10). The sections were then blocked with BLOXALL® Endogenous Alkaline Phosphatase Blocking Solution (VectorLabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... Gold grids (200-mesh coated with Formvar-carbon film, EMS #FCF200-Au-EC) were made hydrophilic by glow discharge at 5 mA for 60 s (easiGlow ...
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bioRxiv - Plant Biology 2024Quote: ... Small pieces of samples (about 2-3 mm3) were fixed with 3.0 % glutaraldehyde (EMS 16220) plus 1 % paraformaldehyde (Merck P6148 ...
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bioRxiv - Plant Biology 2024Quote: Shoot apices at different developmental stages were dissected under a stereomicroscope and fixed with 4% (v/v) paraformaldehyde (PFA; Electron Microscopy Sciences). The fixed samples were washed twice for 1 min in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... samples were en bloc stained in 1% aqueous uranyl acetate (Electron Microscopy Sciences, Hatfield, PA) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were briefly fixed with 0.1% [w/vol] PFA (Electron Microscopy Sciences, Hatfield, PA) and separated by centrifugation at 500g for 5 minutes ...
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bioRxiv - Microbiology 2024Quote: ... subtilis colonies were inoculated into 5 mL of LB medium supplemented with 5 µL of ethyl methanesulfonate (EMS), and cultured at 37°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... Three to four microliters of rsVLP or rsVLP-compound-GSH mixture were applied to either glow-discharged C-flatTM holey carbon copper grids (product No. CF312, Electron Microscopy Sciences) for the PV1-SC6b yeast sample or Lacey carbon copper grids with an ultra-thin carbon support film (product No ...
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bioRxiv - Microbiology 2024Quote: ... coated hydrophobic printed well slides (EMS, 63425-05) with Vectashield (Vector Labs ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... 1 mL of culture was fixed at ambient temperature in the dark (1-2 h) with paraformaldehyde 3.8% (PFA, Electron Microscopy Sciences). Cells were then centrifuged 3 min at 8000 g and washed 3 times with 500 µL PBS ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Samples were then embedded in pure Spurr resin (EMS), followed by polymerization for 48 h at 60°C ...
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bioRxiv - Microbiology 2024Quote: ... For SEM analysis these samples were fixed using the scanning electron microscope (SEM) fixation buffer (2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4, Electron Microscopy Sciences, USA) 3 ...
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bioRxiv - Microbiology 2024Quote: Copper mesh grids (Formvar/Carbon 300, Electron Microscopy Sciences) were loaded with 5 µL samples for 60 seconds ...
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bioRxiv - Microbiology 2024Quote: ... immediately stained with 1% uranyl acetate (Electron Microscopy Sciences, 22400-1) for 30 seconds ...
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bioRxiv - Microbiology 2024Quote: ... All chemicals were from Electron Microscopy Sciences, and solvents were from Sigma.
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bioRxiv - Immunology 2024Quote: Neutrophils migrating under the agarose for 1 h in 8 well chamber dishes were fixed using 4% paraformaldehyde (PFA, EMS #) in PHEM buffer for 20 min at 37 °C followed by agarose removal from the wells ...
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bioRxiv - Microbiology 2024Quote: ... The inactivated samples were deposited on square 200-mesh Formvar carbonated copper-coated grids (Electron Microscopy Sciences; FCF200-Cu-50) and contrasted using 2% uranyl acetate (Canemco and Maivac) ...
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bioRxiv - Microbiology 2024Quote: ... Five microliter droplets were then incubated on carbon grids (Electron Microscopy Sciences) for 2 min at 25 °C and negatively stained with uranyl acetate ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed with 16% light sensitive paraformaldehyde (PFA, Electron Microscopy Sciences Inc, Hatfield, PA) at a final concentration of 5% PFA at 37°C for 10 min ...
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bioRxiv - Immunology 2024Quote: ... followed by 10 min of fixation with 4% paraformaldehyde (PFA) (cat. 15710, Electron Microscopy Science) at 37°C.
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bioRxiv - Immunology 2024Quote: ... The cells were washed in FACS buffer and fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences). Cells were stored at 4°C in the dark until acquisition ...
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bioRxiv - Immunology 2024Quote: ... Sections were washed thrice in PBS and then mounted with Fluoromount-G (Electron microscopy sciences, #1798425). Controls included ...
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bioRxiv - Microbiology 2024Quote: ... were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15710-S) for 25 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: Two 20-µL droplets of deionized (DI) water and two droplets of UranyLess EM contrast stain (Electron Microscopy Science) were placed on a parafilm surface ...
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bioRxiv - Microbiology 2024Quote: ... Polymerization was induced with addition of 2 mM GTP and reactions were incubated for 15 min prior to spotting on glow-discharged carbon-coated copper grids (Electron Microscopy Sciences, Hatfield, PA). Grids were blotted and stained twice with 0.75% uranyl formate for 2 min ...
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bioRxiv - Neuroscience 2024Quote: ... dissociated cells (Trypan Blue exclusion) were counted and plated on 25 mm round coverslips (Electron Microscopy Science, 72196-25) coated with Poly-L-Ornithine Solution (0.01% ...
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bioRxiv - Neuroscience 2024Quote: ... then 4% PFA (Electron Microscopy Sciences, Cat#15710-S) in 1x PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed twice with PBS on ice and fixed with 4% paraformaldehyde (EMS) at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... a QuantiFoil Au R0.6/1 grid (Electron Microscopy Sciences) was glow discharged for 30 sec at 20 mA with a glow discharge cleaning system (PELCO easiGlow) ...
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bioRxiv - Molecular Biology 2024Quote: ... elegans were rotated in M9 buffer solution containing 1% ethyl methyl sulfonate (EMS) solution for 4 hours at room temperature and then washed 4x in M9 ...
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bioRxiv - Microbiology 2024Quote: ... the sample was washed with 0.075% ruthenium red in 0.1 M cacodylate buffer followed by distilled water and then dehydrated in a graded ethanol series before being embedded in an epon resin (Embed 812 embedding kit, EMS), which was polymerized for 24h at 60°C.
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bioRxiv - Molecular Biology 2024Quote: ... an QuantiFoil Au R0.6/1 grid (Electron Microscopy Sciences) was glow discharged for 30 sec at 20 mA with a glow discharge cleaning system (PELCO easiGlow) ...
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bioRxiv - Microbiology 2024Quote: ... followed by dehydration with a series of ethanol washes and embedding in LR White resin (Electron Microscopy Sciences). Thin sections were blocked with 5% non-fat milk (Becton ...
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bioRxiv - Molecular Biology 2024Quote: ... 35 µl of 1.4X refolding buffer comprising 400 mM sodium cacodylate pH 7.2 (Electron Microscopy Sciences) and 6 mM magnesium chloride (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... a 3 μL sample was placed on a glow-discharged carbon film 300-mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Molecular Biology 2024Quote: ... immediately fixed in cold 4% paraformaldehyde (Electron Microscopy Sciences, #15710-S) in 1xPBS (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... by using a blue light for the excitation wavelength of GFP (NIGHTSEA stereo microscope fluorescence adapter, SFA-RB royal blue, Electron Microscopy Sciences, Hatfield, PA) in front of a DLSR camera (Nikon D5300 ...
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bioRxiv - Neuroscience 2024Quote: ... with one 4 mm cover glass (round, #1.5 thickness, Electron Microscopy Sciences) adhered to the bottom ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isofluorane and perfused intracardially with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences). Brain was left to fix on PFA for 2h at room temperature (RT ...
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bioRxiv - Neuroscience 2024Quote: ... the dura was thinned using an Ultra Needle (Electron Microscopy Sciences), and a well was formed with Kwik-Cast around the recording site to hold agarose and phosphate-buffered saline.
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bioRxiv - Neuroscience 2024Quote: Organoids and assembloids were fixed in 4% paraformaldehyde (PFA in PBS, Electron Microscopy Sciences) overnight and subsequently washed three times in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, USA) for 15 minutes at RT ...
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bioRxiv - Microbiology 2024Quote: Samples were chemically fixed using 2.5% glutaraldehyde (Electron Microscopy Sciences # 16216) in fish water ...