Labshake search
Citations for Electron Microscopy Sciences :
851 - 900 of 7095 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... and embedded in Embed-812 (Electron Microscopy Science, PA).39 Sections were cut in a horizontal plane parallel to that of the membrane to provide panoramic views of the cells ...
-
bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and transcardial perfusion was performed with 10 mL of 1x PBS followed by 10 mL of ice cold 4% PFA (EMS, #15710-S) in 1x PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... slides were coverslipped with Fluorogel in TRIS buffer (EMS) and stored at 4℃ until time of imaging ...
-
bioRxiv - Neuroscience 2024Quote: Mice were anesthetized by IP injection of Avertin and transcardially perfused with PBS followed by 4% paraformaldehyde (Electron Microscopy Sciences) in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then washed in acetone and finally embedded in a mixture of resin/acetone first and then in pure Epon 812 resin (Embed 812-EMS) overnight ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS for 20 mins at RT ...
-
bioRxiv - Molecular Biology 2024Quote: Whole mouse hindlimbs were fixed in 1.5% glutaraldehyde/1.5% paraformaldehyde (Electron Microscopy Sciences) in Dulbecco’s serum-free media containing 0.05% tannic acid (Sigma) ...
-
bioRxiv - Physiology 2024Quote: ... pH 7.4 (Electron Microscopy Sciences, CAT#16537-07).
-
bioRxiv - Neuroscience 2024Quote: ... and finally embedded in Epon 812 resin (Electron Microscopy Sciences). 70nm ultra-thin sagittal sections were obtained along the medial edge of the corpus callosum using an ultramicrotome (UC7 ...
-
bioRxiv - Neuroscience 2024Quote: ... and mounted onto 2×1 mm Formvar-coated copper slots (Electron Microscopy Sciences). Ultra-thin sections were then grid stained with 2% uranyl acetate for 12 minutes and Reynolds’ lead citrate stain for 6 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... infiltrated with Epon resin (Electron Microscopy Sciences) and embedded and polymerized at 60 °C for 48 hours ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were then post-fixed with 1% glutaraldehyde in 0.15 M Sorensen’s phosphate buffer (SPB; Electron Microscopy Sciences) for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then washed 3 times in ddH2O and incubated on ice for 1 hour in a solution composed of 1.5% potassium ferrocyanide and 1.5% osmium tetroxide (EMS) diluted in ddH2O ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were dehydrated in graded ethanol and then flat-embedded in Epoxy 812 medium (Catalogue no.14121; EMS, United-States) for 48h at 60°C ...
-
bioRxiv - Neuroscience 2024Quote: ... then samples were embedded for 2 h with 1:1 acetone and epon resin (Embed-812, EMS), followed by 100% epon resin at 60°C for 48 h ...
-
bioRxiv - Neuroscience 2024Quote: ... animals were anesthetized in 0.02% MS-222 and then immersed in 4% PFA (diluted from 16% PFA in 1xPBS, Cat # 15710, Electron Microscopy Sciences, Fort Washington, PA) using a Pelco Biowave Pro microwave (Model 36700 ...
-
bioRxiv - Neuroscience 2024Quote: ... Attached iAstrocytes were either fixed by 3.7% formaldehyde (FA; Electron Microscopy Sciences, 15681) for 10-15 minutes at RT and stored at 4°C in 1X PBS for immunofluorescent staining ...
-
bioRxiv - Neuroscience 2024Quote: ... 1 min each) slides were mounted with a coverslip and DPX mounting medium (Electron Microscopy Sciences). Details of reagents are listed in Table 1.
-
bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and then perfused transcardially with room temperature (RT) phosphate buffered saline (PBS) with heparin (0.02 mg/mL) and then ice-cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, catalog no: 15714). Brains were dissected and post-fixed in 4% PFA overnight at 4°C and cryopreserved in 30% sucrose in PBS for ∼2 days at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Retinas processed in this way were embedded in a mixture of EM-bed/Araldite (Electron Microscopy Sciences). Sections (1 μm thick ...
-
bioRxiv - Neuroscience 2024Quote: ... and 2.5% formaldehyde (Electron Microscopy Sciences), in 0.1M cacodylate buffer pH 7.4 containing 0.1 mM EGTA for 10 minutes at RT with gentle flushing ...
-
bioRxiv - Neuroscience 2024Quote: ... and then stained with 2% uranyl acetate (Electron Microscopy Sciences) and lead citrate (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then gently washed with phosphate buffer saline (PBS) at room temperature for 5 minutes and then fixed with 4% paraformaldehyde (PFA) (cat:15710-S, Electron Microscopy Science) for 20 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... then perfused with 40 mL fresh 4% paraformaldehyde (PFA, #19208, Electron Microscopy Sciences, EMS) in 0.1M phosphate-buffered saline (pH 7.4) ...
-
Deep brain stimulation induces white matter remodeling and functional changes to brain-wide networksbioRxiv - Neuroscience 2024Quote: ... were mounted on Formvar/carbon coated slot grids (EMS, FCF2010-Cu) or 100 mesh Cu grids (EMS ...
-
Deep brain stimulation induces white matter remodeling and functional changes to brain-wide networksbioRxiv - Neuroscience 2024Quote: ... or 100 mesh Cu grids (EMS, FCF100-Cu). To evaluate the myelin thickness ...
-
bioRxiv - Neuroscience 2024Quote: Gels were then moved to a poly-l-lysine coated cover glass-bottom petri dish (Electron Microscopy Sciences, catalog #70673, Hatfield, PA) with a paintbrush and incubated for expansion in ddH2O for 3∼4 rounds of 20-minute expansion session until expansion plateaued (around 60-100 minutes) ...
-
bioRxiv - Neuroscience 2024Quote: ... Poly-l-lysine coated chambers and coverslips were prepared by washing glass microscope slides and glass coverslips with acid alcohol (1% HCl in 70% ethanol) and placing 1:10 0.1% poly-l-lysine (Electron Microscopy Sciences, catalog #19320, Hatfield, PA) in ddH20 on slide for 1 hour before aspirating off and leaving covered in fume hood overnight (Suvarna SK 2013 ...
-
bioRxiv - Neuroscience 2024Quote: ... Right hemispheres were drop-fixed for 48 hours in 4% paraformaldehyde (Electron Microscopy Sciences) and then placed in 1× PBS (Corning) ...
-
bioRxiv - Neuroscience 2024Quote: ... Devices were fabricated by thoroughly mixing polydimethylsiloxane (PDMS) and a curing agent in a 10:1 ratio by weight (Sylgard 184, Electron Microscopy Sciences Cat. 24236 – 10), degassing the mixture under vacuum ...
-
bioRxiv - Neuroscience 2024Quote: ... and cover slipped with Permount (Electron Microscopy Sciences). Images were taken at 10X on a Zeiss imager M2 brightfield microscope (MBF Biosciences) ...
-
bioRxiv - Neuroscience 2024Quote: ... 2% freshly made paraformaldehyde (EMS, #19202), and 2mM CaCl2 at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... fluorescently tagged goat anti-mouse and anti-chicken secondary antibodies were diluted in PBS and incubated for 30 min and coverslips were mounted using Fluoromount-G mounting medium (Electron Microscopy Sciences).
-
bioRxiv - Neuroscience 2024Quote: ... containing 2.5% glutaraldehyde (EMS, #18220), 2% freshly made paraformaldehyde (EMS ...
-
bioRxiv - Neuroscience 2024Quote: ... and the brain was fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) overnight ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were mounted on microscope slides coated with liquid release agent (EMS, #70880) in 100% resin and cured at 60°C for 2 days ...
-
bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: Cells were fixed using 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, USA) for 20 min at room temperature followed by three washing steps with PBS++ ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences), then de-hydrated overnight in a 30% sucrose PBS solution before cryopreservation in a -80°C freezer ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were fixed on ice with 4% paraformaldehyde (PFA, Electron Microscopy Sciences) and incubated with anti-HA primary antibody (1:1000 ...
-
bioRxiv - Neuroscience 2024Quote: ... cover-slipped with Fluoro-Gel (Electron Microscopy Sciences).
-
bioRxiv - Neuroscience 2024Quote: ... for 10 min and washed with PBS three times and cover-slipped with Fluoro-Gel (Electron Microscopy Sciences, catalog no: 17985-10).
-
bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with pentobarbital (Fatal-Plus, Vortech Pharmaceuticals) and intracardially perfused with ∼50 ml of 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... 1 min each) slides were mounted with a coverslip using DPX mounting medium (Electron Microscopy Sciences).
-
bioRxiv - Neuroscience 2024Quote: ... and coverslips were mounted on glass microscope slides using Fluoro-Gel Mounting Medium (Electron Microscopy Sciences, 17 985-30). Images were taken with Nikon A1R HD scanning confocal microscope using a 40x objective and analyzed using Imaris 10.0 software (Oxford Instruments) ...
-
bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
-
bioRxiv - Neuroscience 2024Quote: ... at the time of brain microdissection the spinal cord was rapidly dissected and immersed in 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA). For assessment of phospho-cJUN and ATF3 expression animals were perfused with 4% PFA ...
-
bioRxiv - Neuroscience 2024Quote: ... The charge delay was set at 350 ms and coil position was monitored using the Softaxic 3.4.0 neuronavigation system (EMS, Bologna, Italy). To attenuate the contamination of TEPs with sensory artifacts ...
-
bioRxiv - Neuroscience 2024Quote: ... Retinas were washed and mounted ganglion cell layer up in Fluorogel in TRIS buffer (Electron Microscopy Sciences). As previously described RBPMS+ and pJUN+ cells were quantified following collection of eight 40x fields per retina ...