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Citations for Electron Microscopy Sciences :
851 - 897 of 897 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Retinas were washed and mounted on microscope slides ganglion cell layer-up in Flourogel in TRIS buffer (Electron Microscopy Sciences, 17985-11).
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bioRxiv - Genomics 2020Quote: Protein ICC was performed as described previously (Martin et al., 2013) on cells fixed in 4% paraformaldehyde or 2% paraformaldehyde (Electron Microscopy Sciences). For double and triple immunocytochemistry using antibodies to methylated DNA ...
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bioRxiv - Developmental Biology 2020Quote: The selected MC12 cell lines were plated on a 35-mm glass bottom dish (IWAKI) and fixed with 4% formaldehyde (Electron Microscopy Sciences) in 250 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Resuspended cells were loaded on Concanavalin A-treated coverslip (#1.5 Micro Coverglass −25 mm Diameter, Electron Microscopy Sciences, Cat. No. 72225-01) assembled on imaging cell chamber (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: Cultures were centrifuged and the cells fixed for at least 4 h at room temperature in 10 volumes of 4% paraformaldehyde (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2020Quote: ... Naive T cells were maintained 4 days in IL-7-supplemented medium and then fixed overnight in 2.5% glutaraldehyde (Electron Microscopy Sciences, UK) at 4°C and post-fixed with 1% osmium tetroxide (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2021Quote: ... the entire liquid medium was gently discarded from each dish and the adherent cells were immediately fixed with 4% PFA (Electron Microscopy Sciences), 0.1% glutaraldehyde (G5882 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Microbiology 2022Quote: ... the cells were dehydrated in increasing concentrations of ethanol (50%-100%) at room temperature and embedded in EPON resin (Electron Microscopy Sciences). Ultrathin sections (70-80 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then fixed for 10 minutes at room temperature in PBSM supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-492). Cells were then washed 3x for 5 minutes in PBS and exchanged into a permeabilization buffer consisting of PBSM + 5 mg/mL BSA (VWR ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were incubated with 200nM Mitotracker for 30 min before fixing them in 4% paraformaldehyde (Electron Microscopy Sciences Cat# 15710, Hatfield, PA) for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were first fixed with 1 mL of the fixative (2% paraformaldehyde [Electron Microscopy Sciences] dissolved in 250 mM HEPES [pH 7.4]) for 10 min at room temperature (approximately 25°C) ...
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bioRxiv - Neuroscience 2024Quote: ... iPS cell-derived neuronal cultures were fixed using 4% paraformaldehyde diluted in 1 PBS from a 16% aqueous solution (Electron Microscopy Sciences) for 20 min and then permeabilized with 0.2% Triton X-100 (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed three times with pre-warmed phosphate-buffered saline (PBS) before being fixed in 4% paraformaldehyde (Electron Microscopy Sciences #15710) in PBS for 15 mins at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The sapphire disks with affixed cells were flat-embedded onto a Teflon-coated glass microscope slide and covered with a Thermanox coverslip (Electron Microscopy Sciences). Resin was polymerized at 60°C for 48 h and the sapphire disks were excised ...
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bioRxiv - Cell Biology 2023Quote: ... and PFF treatment was conducted at the indicated concentrations and durations prior to fixing cells with 4% Paraformaldehyde (EMS catalog 15710-S) in DPBS (Corning catalog ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were dissociated and 75,000 cells were replated overnight in 2D on 12 mm round #1 coverglass (Electron Microscopy Sciences 72231-01) coated with 75 μg/ml Poly-D-lysine followed by 3% Matrigel ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed with PBS and fixed in 1 mL ice cold 4% PFA (Electron Microscopy Science, Hatfield, PA, USA) diluted in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the cell suspension was pipetted onto precoated Laminin coverslips at 150-170 μl on each coverslip (Cat#: 72298-01, Electron Microscopy Science), placed in 35 mm petri dishes ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times with 1X PBS and then mounted with Fluoro-Gel mounting media (Electron Microscopy Sciences #50-247-04). Immunostained neurons were imaged on a Nikon Eclipse Ti-E inverted epifluorescence microscope equipped with CFI Plan Apochromat VC 100X oil (NA 1.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in 100 mM sucrose and the cell suspension distributed onto slides (Matsunami, S9901) covered with 1% PFA in H2O (Electron Microscopy Science, 15713) with 0.2% Triton X-100 (pH 9.2-9.4) ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips containing the homozygously expressing SNAP-NOIs cells were pre-fixed with 2.4% [w/v] formaldehyde (FA; Electron Microscopy Sciences; cat.# 15710) in 1x PBS (+Ca2+/Mg2+) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with wheat germ agglutinin for 10 min prior to fixing with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min and permeabilization with 0.5% Triton-X 100 for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... At that time cells were washed once with sterile 1x PBS and then fixed with 4% PFA solution (Electron Microscopy Sciences; Ref# 15710) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: The cells from the one-step growth experiment were immobilized by spotting 20 μl of fixed cell suspension on SuperFrost Plus glass slides (Electron Microscopy Sciences, USA), on which silicone isolators (Grace Bio-Lab ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The cell pellets were then infiltrated with a 50:50 mixture of acetone and embedding resin (Embed 812, Electron Microscopy Sciences, Hatfield, PA) for 72 hours ...
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bioRxiv - Immunology 2023Quote: ... the whole lung single-cell suspension was prepared and stained as above and fixed by 3.2% paraformaldehyde (PFA) (Electron Microscopy Sciences, #15714-S) for 15 min ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are washed with culture medium and fixed for 2 minutes with room temperature fixative (4% EM grade paraformaldehyde (Electron Microscopy Sciences #19202) + 0.1% EM grade glutaraldehyde (Ted Pella ...
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bioRxiv - Cell Biology 2024Quote: ... Images of fixed cells were taken on either the LSM or spinning disk confocal microscopes at 40x after incubation in 4% paraformaldehyde (Electron Microscopy Sciences, #15710) for 5 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... proliferating MuSC and C2C12 cells were cultured on pre-coated glass coverslips and then fixed in 4% paraformaldehyde/PBS (Electron Microscopy Sciences, Hatfield, PA). RNA hybridization and signal development were carried out using Basescope assay (Advanced Cell Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Cell Biology 2022Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... RPE1 cells were transiently transfected with pDestEGFP-NBR1(D50R) (Kirkin et al., 2009) and grown on photo-etched coverslips (Electron Microscopy Sciences, Hatfield, USA). Cells were fixed in 4% formaldehyde ...
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bioRxiv - Cell Biology 2021Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... about 5 × 104 of Tet-ON-GFP-PCNT (854-1960) cells were seeded onto each 12-mm circular coverslip (72230-01, Electron Microscopy Sciences, Hatfield, PA) in a 24-well plate and treated with 1 µg/ml Dox for 24 h to induce the formation of PCNT (854-1960 ...
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bioRxiv - Immunology 2021Quote: ... 48-hour activated T cells from PRMT5fl/fl and T-PRMT5Δ/Δ mice were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min in V-bottom plates (Costar ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Developmental Biology 2023Quote: ... or any of the four possible distributions at the 4-cell stage (ABa and P2; ABa and EMS; ABp and P2; ABp and EMS), were more likely to harbor sperm-contributed centrioles using the χ2 test as implemented in the library SciPy ([https://scipy.org/citing-scipy/]) ...
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bioRxiv - Genomics 2020Quote: ... cells were fixed by adding the fixative directly to the medium to a final concentration of 4% paraformaldehyde (PFA) (Electron Microscopy Sciences; cat. no. 15714) using the automated liquid handler BlueWasher (BLUE CAT BIO) ...
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bioRxiv - Cell Biology 2021Quote: Cells were cultured on cell-tight C-2 cell disks (Sumitomo Bakelite, Tokyo, Japan; #MS-0113K) and fixed for 2 h on ice in 2.5% glutaraldehyde (Electron Microscopy Science, Hatfield, PA; #111-30-8) in 0.1 M phosphate buffer ...