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Citations for Electron Microscopy Sciences :
801 - 850 of 897 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed in 0.1 M cacodylate buffer (pH 7.4) with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed in PBST and sealed with DAPI containing mounting medium (Dapi-Fluoromount-G™, Electron Microscopy Sciences). Images were taken by Zeiss 880 confocal microscopy ...
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bioRxiv - Biophysics 2024Quote: ... Cells were seeded onto glow-discharged and Fibronectin-coated Quantifoil grids (R1/4, Au 200-mesh grid, Electron Microscopy Sciences). Following this seeding ...
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bioRxiv - Developmental Biology 2023Quote: ... or any of the four possible distributions at the 4-cell stage (ABa and P2; ABa and EMS; ABp and P2; ABp and EMS), were more likely to harbor sperm-contributed centrioles using the χ2 test as implemented in the library SciPy ([https://scipy.org/citing-scipy/]) ...
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bioRxiv - Biochemistry 2022Quote: ... U2OS cells were washed once with PBS and immediately fixed by 4% EM-grade paraformaldehyde (Electron Microscopy Science, Hatfield, PA) for 10 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... and the resulting cell pellets were fixed using a mixture of 2% [v/v] paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) and 2% [v/v] glutaraldehyde (Agar Scientific ...
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bioRxiv - Bioengineering 2024Quote: Cells were cultured on NP hydrogels for 48 hours before fixing with 4% (w/v) paraformaldehyde (Electron Microscopy Sciences, 15710) for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... For all experimental approaches cells were washed with PBS once and fixated in 4% PFA (Electron Microscopy Sciences, #15714-S) in PBS (Thermo Fischer ...
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bioRxiv - Cell Biology 2024Quote: HeLa and MEF cell lines cultured on glass coverslips were fixed for 10min using 4% paraformaldehyde (Electron Microscopy Sciences #15710), in PBS followed by permeabilization for 20minutes with 0,1% Triton X-100 (Sigma-Aldrich #T8787 ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed with 100 µl of warm PBS and fixed with 4% methanol-free paraformaldehyde (PFA) (Electron Microscopy Sciences) for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: BF PolIE RNAi cells were grown with or without doxycycline for 24 hours then incubated with 2 mM of ethylmethanesulfonate (EMS; Sigma) or DMSO (vehicle ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell samples were first stained for viability with cisplatin (201064, Fluidigm) and then fixed with 1.5% paraformaldehyde (15710, Electron Microscopy Sciences) at room temperature for 10 minutes followed by two washes with Cell staining medium (CSM ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µL of resuspended cells were pipetted onto agarose pads in 13 x 1 mm silicone isolator wells (Electron Microscopy Sciences) and covered with a No ...
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bioRxiv - Microbiology 2020Quote: ... virus- or mock-infected cells were detached using 0.06% trypsin-EDTA in phosphate-buffered saline (PBS) and fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in PBS for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Media was aspirated and cells were washed with PBS at RT once before being incubated with 4% PFA (Electron Microscopy Sciences) made up in PBS with the addition of 4% sucrose at RT for 15 mins ...
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bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were taken prior to the experiment (‘Pre’) and at each time point by diluting 50 μl of cells 1:1 with 2% glutaraldehyde (EMS, 16220) and incubating at 4°C to allow cells to sediment ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then rinsed 3 times on ice with ice-cold PBS and immediately fixed in 4% paraformaldehyde (EMS, 15710-S) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: 100.106 haploid HAP1 cells at 60 % confluency were treated for 72 h with 0.3 mg/mL ethyl methanesulfonate (EMS, Sigma-Aldrich) directly added to the cell medium ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2020Quote: ... The mitotic cells were collected by mitotic shake-off and then seeded onto round 25 mm cover glasses (Electron Microscopy Sciences) and placed onto the cell-holding chamber of an LSM510-Meta confocal microscope equipped with a CO2 incubator and a heating chamber ...
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bioRxiv - Cell Biology 2021Quote: ... cells were gently washed with 30 μL of PBS and fixed with 1% (v/v) paraformaldehyde (Electron Microscopy Sciences 15710-S) diluted in PBS at room temperature for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with 1X PBS and fixed in 1X PBS containing a mixture of 4% paraformaldehyde (Electron Microscopy Sciences, 15710) and 0.2% Glutaraldehyde (Euromedex ...
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bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Microbiology 2024Quote: ... Parasites 8 h after induction of the knock sideways (with 250 nM rapalog) and the control cells grown without rapalog were Percoll-enriched and fixed with 2.5 % glutaraldehyde (Electron Microscopy Sciences) in 50 mM cacodylate buffer pH 7.4 for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed with cold PBS for 1 minute and then fixed with 4% PFA (Electron Microscopy Sciences: 157-4) for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were dehydrated in graded series of ethanol on ice and embedded in LR White (Electron Microscopy Sciences, Hatfield, PA). The samples were polymerized under UV light (360nm ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Molecular Biology 2023Quote: HEK-293 cells (60%-70% of confluency) were fixed by 2.5% glutaraldehyde and 2% paraformaldehyde (both from Electron Microscopy Science, Inc.) solution for 1 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots (10 µL) of fixed cells were then loaded onto PTFE printed glass slides (cat#63429-04 from Electron Microscopy Sciences) with 4-mm diameter wells and allowed to air-dry ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured on 12mm round coverslips (corning) and were fixed with 4% EM-grade paraformaldehyde (Electron Microscopy Science, Hatfield, PA) in PBS pH7.4 for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with treatments for 20 min at 37°C and immediately fixed by addition of paraformaldehyde (Electron Microscopy Sciences) to 3.7% ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed thrice with PBS and smear dried on a Poly-L-lysine coated slide (Electron Microscopy Science: 63410), mounted with Prolong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... RAW264.7 and THP-1 cells were thawed and seeded as mentioned earlier on 6 well plates containing #1.5H glass cover slips (Electron Microscopy Sciences). Cells were grown and maintained as described above ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature with 4% PFA (Electron Microscopy Sciences) diluted in PBS and permeabilized with 70% ethanol overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed once with 1X DPBS(-) and fixed with 4% PFA diluted from a freshly opened 16% PFA (Electron Microscopy Sciences) ampoule ...
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bioRxiv - Developmental Biology 2021Quote: SMG organoid and cell cultures grown on Nuclepore filters (Cytiva #110405) or glass were fixed with 4% paraformaldehyde (Electron Microscopy Sciences #15710) in 1xPBS for 20 minutes or in -20°C methanol for 18 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Migrated cells attached to the lower surface of the inserts were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Incubation solution was then removed and cells were fixed with a 3.2% Paraformaldehyde solution made in PBS (Electron Microscopy Sciences, Hatfield, PA) for 15 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed for 10 or 30 (if infected with SARS-CoV-2) min in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences).
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bioRxiv - Biophysics 2022Quote: U-2 OS cells expressing pRFPs-mito were seeded into 96-well optical polymer base microplates and fixed with 2% paraformaldehyde (Electron Microscopy Sciences) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... bigemina indirect immunofluorescence assays were performed on >10% parasitemia bovine red blood cells by air drying drops in 10-well slides (Electron Microscopy Sciences), followed by 100% methanol fixation for 5 min at -20°C ...
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bioRxiv - Microbiology 2019Quote: ... Gelatine blocks were prepared by spinning down this cell suspension at room temperature for 10 min at ∼ 5000 × g and an aliquot of the cell pellet was taken and made it to adhere at the bottom of the gelatine capsule (Electron Microscopy Sciences) and then capsule was filled with 100% LR White resin ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed with PBS (not containing Mg2+ and Ca2+) thrice and fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) in PBS for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Sorted germ cells and SPZEJ were processed as described previously (51, 71) and attached to UltraStick/UltraFrost Adhesion slides (Electron Microscopy Sciences). Samples were fixed in 4% PFA in PBS for 15 min before antigen retrieval in three consecutive 5-min incubations with boiling citrate (10 mM at pH 6) ...
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bioRxiv - Cell Biology 2022Quote: ... the unroofed cells were placed in fresh 4% PFA (for immunofluorescence) or in 2% glutaraldehyde (for TEM, EM grade, Electron Microscopy Science) for fixation at 4C.
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bioRxiv - Microbiology 2020Quote: ... infected cells were washed twice with PBS buffer (pH 7.4) and fixed with a 4% methanol-free paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS buffer (pH 7.4 ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml ...
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bioRxiv - Immunology 2021Quote: ... An aliquot of mononuclear and granulocyte cells was processed in a cytospin and stained using Diff Quick (26096-25, Electron Microscopy Sciences). Cell differentials were counted ...