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Citations for Electron Microscopy Sciences :
851 - 883 of 883 citations for Diethyl 2 chlorothiazol 5 yl methylphosphonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 3 µL complex was mixed with 1 µL 20 µM lauryl maltose neopentyl glycol (LMNG) and immediately applied to glow discharged C-Flat™-2/1-3Cu-T50 (Electron Microscopy Sciences) holey grids at 4 °C and 100 % humidity inside a Vitrobot Mark IV ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were rinsed in 0.15 M cacodylate buffer 3x for 20 minutes each before incubation in a 2% OsO4 in 0.15 M cacodylate buffer for 1.5 hours in the dark (19152, Electron Microscopy Sciences, Hatfield, PA, USA). Post-OsO4 incubation ...
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bioRxiv - Neuroscience 2024Quote: ... using a Leica ARTOS 3D ultramicrotome, 2-3 74 nm thick sections were collected (4 levels, ∼ 8-10 μm apart) on copper grids (EMS, cat# G150-Cu) and imaging on TEM - 120kv JEOL 1400-Flash equipped with a 4k Gatan OneView digital camera ...
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bioRxiv - Neuroscience 2024Quote: ... at 1 : 1 for 30 min and 2 : 1 for 30 min and twice with pure Durcupan (Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min each ...
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bioRxiv - Developmental Biology 2024Quote: About ∼50 million H1-GATA6+/+ cells at day 2 or day 4 of cardiac differentiation were fixed with 1% formaldehyde (Electron Microscopy Sciences 15710) for 8 min at RT with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Neuroscience 2021Quote: ... the grid was stained one time for 3 minutes and three times for 1-minute each using 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Excess stain was wicked away in between the steps ...
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bioRxiv - Microbiology 2020Quote: Double CsCl gradient-purified HAdV particles were adhered to Collodion and 2% (v / v) amyl acetate film-covered grids (300 mesh Formvar/carbon-supported copper support films, Electron Microscopy Sciences, Hatfield, USA). Viral particles were negatively stained with 2% (v / v ...
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bioRxiv - Cell Biology 2021Quote: ... were applied to carbon-coated and glow discharged (2 minutes at 7x10-1 mbar) 300-mesh copper EM grids (Electron Microscopy Sciences; CF300-CU) and incubated for four minutes at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Microbiology 2022Quote: ... thick resin sections (250 nm) were cut and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were decorated with fiducial marker and stained with Hoechst to visualize nuclear regions ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5mM Mg(OAc)2 wash buffer to an optical density of 2 A260/mL (corresponding to 40 nM of 80S) and deposited on copper grids (Electron Microscopy Sciences, CF400-CU). Grids were glow-discharged at 15 mA for 30s 4 uL of sample were incubated on the glow-discharged grids for 1min ...
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bioRxiv - Physiology 2024Quote: ... The sections were cover-slipped in Fluoromount-G™ Slide Mounting Medium with DAPI (4′,6- diamidino-2-phenylindole; Electron Microscopy Sciences, Hatfield, PA) and examined under a brightfield microscope to identify irregularities in the luminal surface in eTNAP mice ...
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bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
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bioRxiv - Neuroscience 2022Quote: Postnatal mouse were transcardially perfused with 15ml (P14) or 25ml (>P21) of 2% PFA freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Neuroscience 2021Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) in phosphate buffered saline ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was dehydrated through an ethanol series (70% ethanol, 90% ethanol, 2 x 100% ethanol; 8 minutes each) and mounted in Entellan (Electron Microscopy Science, Hatfield, PA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed for at least 2 hours (at room temperature) to overnight (at 4° C) in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in artificial sea water ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) and 2% PFA in 0.1 M in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Microbiology 2024Quote: ... This concentrate was diluted 1:2 with molecular grade water and 50 µL was fixed with 25% glutaraldehyde (stored at 4°C, Electron Microscopy Sciences, Hatfield, PA, USA) to a final concentration of 0.25% ...
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bioRxiv - Neuroscience 2024Quote: ... 70 nm ultrathin sections were taken and post-stained with 2% aqueous uranyl acetate and lead citrate (22400, 22405, 17800, 17900, 21140, Electron Microscopy Sciences, Hatfield, PA, USA). Samples were imaged on a TEM (Jeol JEM-1400 Plus ...
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bioRxiv - Neuroscience 2024Quote: mice were perfused 7 dpI and 10 dpl with 25 mL of 2% PBS-paraformaldehyde freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Biochemistry 2024Quote: ... 4 µl of sample were added to freshly glow-discharged 300 mesh 2/1 µm C-flat copper grids (Electron Microscopy Sciences, Hatfield, Pennsylvania, USA), blotted for 10 s at blot force 4 at 100% relative humidity and 4°C before being plunge frozen in a liquid mix of 37% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... after drying (5 min, at 37°C in a non-humid oven) and gentle fixation with ice-cold 4% Paraformaldehyde (Electron Microscopy Sciences, #15700; 15 min, at 4°C). Nonspecific binding was blocked by incubating the sections in 5% normal donkey serum in Tris-buffered saline (1 hour ...
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bioRxiv - Cell Biology 2021Quote: Cells were cultured on cell-tight C-2 cell disks (Sumitomo Bakelite, Tokyo, Japan; #MS-0113K) and fixed for 2 h on ice in 2.5% glutaraldehyde (Electron Microscopy Science, Hatfield, PA; #111-30-8) in 0.1 M phosphate buffer ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Pathology 2021Quote: ... Sections were stained with hematoxylin and eosin (H&E; Fisherbrand™ 517-28-2, Waltham, MA) or safranin-O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA), following manufacturer protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The sample attached to the grid was stained by immediately tapping the grid on 2% w/w uranyl acetate (Electron Microscopy Sciences, Hatfield, PA, USA, Cat. No. 22400) solution droplets twice ...