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Citations for Electron Microscopy Sciences :
651 - 700 of 883 citations for Diethyl 2 chlorothiazol 5 yl methylphosphonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... cells were rinsed with PBS containing calcium and magnesium and fixed using 2% paraformaldehyde (PFA) (Electron Microscopy Sciences # 15710) in PBS for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... high Grid-50 Glass Bottom is a 35 mm then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS Cat# 16000) and 4% pFormaldehyde (EMS Cat# 15700 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µl) and dried overnight on Formvar/Carbon-coated hexagonal copper mesh grids (FCF200H-CU-TB, Electron Microscopy Sciences). The grids were observed on a transmission electron microscope JEM 1010 (Jeol ...
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bioRxiv - Plant Biology 2024Quote: A glow discharged (30 s, 25 mA) Quantifoil 2/1 Au 300 mesh electron microscopy grid (Electron Microscopy Sciences) was mounted in a Thermo Fisher Vitrobot Mark IV system set to 100% humidity and 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: Enucleated eyes were fixed for 2 hours at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science, Hatfield, PA) prepared in phosphate-buffered saline (1× PBS ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were stained for 25 seconds with a 2% aqueous solution of uranyl acetate from Electron Microscopy Sciences. Following staining ...
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bioRxiv - Microbiology 2024Quote: ... Clarified cell supernatant was then fixed with 2% PFA (10mL of 8% EM grade PFA, Electron Microscopy Science, USA). The fixed viral particles were then purified by ultra-centrifugation at 124000g for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... one set of control coverslips was washed with PBS before being fixed in 2% PFA (Electron Microscopy Science, 15710) for 15 min at RT ...
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bioRxiv - Genomics 2024Quote: ... we anecdotally observed higher ATAC-seq levels in promoters of genes specifically expressed in their somatic sister cells (e.g. high accessibility on med-1 and med-2 promoters in EMS and P2 ...
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bioRxiv - Cell Biology 2024Quote: ... culture aliquots were taken and mixed 1:2 with Paraformaldehyde 3% Glutaraldehyde 0.35% In 0.1M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Cells were fixed for 30-60 min at RT and diluted in low fluorescence media ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were diluted 10-fold in liposome buffer and 5 μL was applied to glow-discharged 400 mesh copper grids coated with formvar/carbon film (Electron Microscopy Sciences). After 1 min ...
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bioRxiv - Neuroscience 2020Quote: ... Shi/Shi:Rag2-/- mice grafted with control and RRMS hiOLs (n=4 per group) were perfused with 1% PBS followed by a mixture of 4% paraformaldehyde/5% glutaraldehyde (Electron Microscopy Science) in 1% PBS ...
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bioRxiv - Bioengineering 2021Quote: ... ∼5 μL of each sEV-particle complex was left to adhere onto formvar carbon coated copper Grid 200 mesh (Electron Microscopy Sciences) for 5 mins followed by 5 mins of negative staining with 2% aqueous uranyl acetate ...
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bioRxiv - Neuroscience 2020Quote: ... Each coverslip was mounted on a stub with carbon tape and samples were sputter coated with iridium at 10μA to a thickness of approximately 5 nm (EMS model 150T S). The coverslips were then examined on a Hitachi S4300 SEM (Hitachi High Technologies America Inc. ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... We applied 5 μL RBC-EV sample to a 200 mesh copper grid coated with formvar carbon film (FCF200-Cu, Electron Microscopy Sciences) for 30 seconds ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 µL sample was spotted onto a glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences CF300-Cu-50), incubated for two minutes then gently blotted onto filter paper to remove excess solution ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were then washed three times in PBS for 5 minutes each prior to cover slipping using Fluorogel mounting medium (Electron Microscopy Sciences).
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bioRxiv - Microbiology 2023Quote: ... and 150 mM NaCl buffer and 5 µL was applied to glow-discharged 400 mesh copper grids coated with formvar/carbon film (Electron Microscopy Sciences). Grids were blotted after 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were stained with 10% Uranyl acetate (Sisco Research Laboratory Pvt. Limited, 81405) for 5-min and Sato’s lead citrate (Electron Microscopy Sciences, 17800) stain for 1-min ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of this sample at 2 µg Chl mL−1 was applied to a glow discharged (60 s, 25 mA) 400 mesh Cu grid with 5-6 nm formvar with 3-4 nm carbon (Electron Microscopy Sciences). The sample was negatively stained with 2% (w/v ...
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bioRxiv - Immunology 2024Quote: ... and 5% glycerol and applied to glow-discharged grid with continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences). The grids were stained with 2% uranyl formate (UF) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then gently washed with phosphate buffer saline (PBS) at room temperature for 5 minutes and then fixed with 4% paraformaldehyde (PFA) (cat:15710-S, Electron Microscopy Science) for 20 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of diluted phage solution was applied onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA, USA). Following a 3-minute incubation ...
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bioRxiv - Cell Biology 2024Quote: ... cells grown on 13-mm round poly-L-lysine-coated coverslips were fixed by adding to the culture medium an equal volume of 4% PFA (Electron Microcopy Sciences) and 5% glutaraldehyde (GTA) (Electron Microscopy Sciences) in 0.2 M cacodylate pH 7.4 for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: Wild type (WT) embryos and WT embryos injected with lama1 morpholino were fixed in 2% formaldehyde (Electron Microscopy Science #15710), 2.5% glutaraldehyde (EMS #16220 ...
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bioRxiv - Microbiology 2021Quote: ... Three μL of cell culture were applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Immunology 2022Quote: ... The sample was double-stained with 6 µl of 2% (w/v) uranyl formate (Electron Microscopy Sciences, catalog number 22450) for 5 seconds (first stain ...
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bioRxiv - Immunology 2022Quote: ... a 2 µl aliquot of EV preparation was placed onto a carbon film grid (Electron Microscopy Sciences, Hatfield, PA, USA) for 1 minute ...
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bioRxiv - Synthetic Biology 2020Quote: ... was adsorbed onto formvar-carbon coated copper grids for 2 min and negatively stained with uranyl acetate replacement stain (UAR-EMS) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... The medial shell of the NAc and a piece of tissue spanning the entire VTA were excised and prepared for EM by staining sequentially with 2% osmium tetroxide (EMS) in cacodylate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was quickly extracted and cut into 2 mm coronal slices using a rodent brain matrix (Electron Microscopy Sciences). Slices were placed on ice cold PBS and PFC and VCX were further dissected and collected for RNA extraction ...
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bioRxiv - Biophysics 2020Quote: ... 270 µL of GPMV solution was pipetted from the bottom of the Eppendorf tube and sandwiched between 2 coverslips coated with 0.1% BSA and separated by a 0.5 mm thick silicone isolator (Electron Microscopy Sciences).
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bioRxiv - Microbiology 2023Quote: Immunofluorescence assay was performed on PHA-E-immobilised parasites by fixation with 2% paraformaldehyde and 0.008% glutaraldehyde (Electron Microscopy Sciences) and permeabilisation with 0.1% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed with glutaraldehyde 2.5% in 0.1 M sodium cacodylate buffer supplemented with 2 mM of calcium chloride (Electron Microscopy Sciences), post-fixed with 1% OsO4 and 1.5% potassium ferrocyanide in sodium cacodylate buffer ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed by immersion in Modified Karnovsky’s Fixative (2% Paraformaldehyde and 2.5% Glutaraldehyde in 0.1 M Sodium Cacodylate Buffer, pH 7.4 (Electron Microscopy Sciences (Hatfield, PA), #15960-01) ...
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bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
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bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde in 0.1M Sodium Cacodylate buffer (cat. no. 15960-01 Electron Microscopy Sciences) for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat ...
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bioRxiv - Biophysics 2024Quote: ... 2.5 µl of sample was applied to 300 mesh Quantifoil R 2/1 copper grids (Electron Microscopy Sciences, Hatfield PA) that had been previously glow discharged (15 mA ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were then mounted with DAPI (diamidino-2-phenylindole)-free Fluoro-Gel on glass slides (Electron Microscopy Sciences, USA). Cells were scanned using a 63x oil immersion objective mounted on a confocal microscope (LSM 710 Meta ...
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bioRxiv - Plant Biology 2023Quote: ... and the resulting cell pellets were fixed using a mixture of 2% [v/v] paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) and 2% [v/v] glutaraldehyde (Agar Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... They were subsequently transcardially perfused with 15 ml of 0.1M sodium phosphate buffer pH 7.4 and then 60 ml of 2% paraformaldehyde (15710-S, Electron Microscopy Sciences) 1% glutaraldehyde (G7526 ...
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bioRxiv - Microbiology 2023Quote: Genomic libraries were constructed and sequenced by MiSeq using paired-end sequencing (2 × 300 bp) for 17 strains generated by EMS mutagenesis and one strain ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
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bioRxiv - Genetics 2023Quote: ... the supernatant was discarded and the pellet resuspended in primary fixating buffer (2% glutaraldehyde/0.1 M sodium cacodylate buffer, pH 7.4, Electron Microscopy Sciences) for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed in 0.1 M cacodylate buffer (pH 7.4) with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... EVs were fixed by positioning the grid on top of a 20 µL drop of 2% paraformaldehyde (EMS, 157-4) for 10 minutes ...