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Citations for Electron Microscopy Sciences :
701 - 750 of 757 citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Leaf tissue was collected from the interveinal region of the youngest fully expanded leaves and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, EMS) and 2.5 % paraformaldehyde (Ted Pella Inc) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by ice-cold EM fixative (1.25% glutaraldehyde [Electron Microscopy Sciences, 16220], 2% paraformaldehyde [Electron Microscopy Sciences, 19210], 0.1M sodium cacodylate buffer). Brains were removed and stored for 8 days in EM fixative at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed with pre-warmed (37°C) PBS and permeabilized for 2 minutes using 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37 °C ...
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bioRxiv - Biophysics 2024Quote: ... These cells in the log-growth phase were collected and deposited on glow-discharged Quantifoil grids (R 2/1, Cu 200-mesh grid, Electron Microscopy Sciences), as described previously (5) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were first fixed with 1 mL of the fixative (2% paraformaldehyde [Electron Microscopy Sciences] dissolved in 250 mM HEPES [pH 7.4]) for 10 min at room temperature (approximately 25°C) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Resuspended culture was mixed with 10 nm gold beads as fiducial markers and then applied onto glow-discharged copper R2/2 200 mesh Quantifoil holey carbon grids (Electron Microscopy Sciences) in Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... One end of the tube was clamped with a hemostat and the tube was filled with room temperature 2% paraformaldehyde (PFA) (Electron Microscopy Sciences) with a syringe and metal cannula ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...
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bioRxiv - Microbiology 2023Quote: ... About 4 µl of aerated and hypoxia samples were pipetted on glow-discharged Quantifoil Au extra thick EM-grids (R2/2, 200 mesh, Electron Microscopy Sciences). The extra fluid was blotted (blot force of 7 s and blot time 5 sec ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were then treated with a nitrocellulose solution (1:1 solution of sterile Collodion in 2% amyl acetate and amyl acetate; Electron Microscopy Sciences) and then left to airdry for one hour ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... They were carefully placed on a fine layer of resin between 2 sheets of ACLAR® embedding films (EMS, cat# 50425-25) for polymerization at 55 °C for 72 h ...
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bioRxiv - Microbiology 2023Quote: 3.5 µL of Vipp1 at a final concentration of 60 µM was incubated for 90 seconds on a plasma cleaned holey R2/2 Quantifoil copper grid (Electron Microscopy Science) before plunge freezing in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: Media were formulated as previously described [2] to create disease progression from Normal Fasting (NF) to Early Metabolic Syndrome (EMS or MASLD) and Late Metabolic Syndrome (LMS or late stage MASLD) ...
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bioRxiv - Biophysics 2022Quote: ... The grids were then stained with 50 μL aqueous uranyl acetate solution (2% v/v) (Electron Microscopy Sciences, Philadelphia, USA. Product No: 22400) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... Brains of 3–7day old male flies were dissected in 1X PBS medium (BPP3920, Fisher Sci) and fixed in 2% paraformaldehyde (PFA, Electron Microscopy Sciences #15710) in PBT for 60 min at room temperature (RT) ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Bioengineering 2022Quote: ... VLP samples were fixed with 2% (v/v) glutaraldehyde in water solution on 400 mesh Formvar-coated copper grids (EMS Cat#FF400-Cu). After fixation ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with Aurion blocking solution for one hour and washed 2 × 15 mins with incubation solution (Electron microscopy sciences, Cat # 25558). Then the slices were incubated with goat anti-BirA in incubation solution (1:50 ...
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bioRxiv - Neuroscience 2022Quote: ... Fibril assemblies were next analyzed via transmission electron microscopy at an applied concentration of 0.4 mg per mL, with fibrils absorbed to glow-discharged 300 mesh (Ladd Research Industries, Inc.) and stained with 2% uranyl acetate (Electron Microscopy Sciences, Inc.) in water ...
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bioRxiv - Bioengineering 2022Quote: ... The tethered EVs were fixed in a 2 % glutaraldehyde (MilliporeSigma, Burlington, MA) and 0.1 M sodium cacodylate solution (Electron Microscopy Sciences, Hatfield, PA) for 3 hr ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are washed with culture medium and fixed for 2 minutes with room temperature fixative (4% EM grade paraformaldehyde (Electron Microscopy Sciences #19202) + 0.1% EM grade glutaraldehyde (Ted Pella ...
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bioRxiv - Plant Biology 2024Quote: Leaf tissue was collected from the interveinal region of the youngest fully expanded leaves and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, EMS) and 2.5 % paraformaldehyde (Ted Pella Inc) ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Immunology 2024Quote: ... 3 µL complex was mixed with 1 µL 20 µM lauryl maltose neopentyl glycol (LMNG) and immediately applied to glow discharged C-Flat™-2/1-3Cu-T50 (Electron Microscopy Sciences) holey grids at 4 °C and 100 % humidity inside a Vitrobot Mark IV ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were rinsed in 0.15 M cacodylate buffer 3x for 20 minutes each before incubation in a 2% OsO4 in 0.15 M cacodylate buffer for 1.5 hours in the dark (19152, Electron Microscopy Sciences, Hatfield, PA, USA). Post-OsO4 incubation ...
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bioRxiv - Neuroscience 2024Quote: ... using a Leica ARTOS 3D ultramicrotome, 2-3 74 nm thick sections were collected (4 levels, ∼ 8-10 μm apart) on copper grids (EMS, cat# G150-Cu) and imaging on TEM - 120kv JEOL 1400-Flash equipped with a 4k Gatan OneView digital camera ...
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bioRxiv - Neuroscience 2024Quote: ... at 1 : 1 for 30 min and 2 : 1 for 30 min and twice with pure Durcupan (Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min each ...
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bioRxiv - Developmental Biology 2024Quote: About ∼50 million H1-GATA6+/+ cells at day 2 or day 4 of cardiac differentiation were fixed with 1% formaldehyde (Electron Microscopy Sciences 15710) for 8 min at RT with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Neuroscience 2021Quote: ... the grid was stained one time for 3 minutes and three times for 1-minute each using 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Excess stain was wicked away in between the steps ...
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bioRxiv - Microbiology 2020Quote: Double CsCl gradient-purified HAdV particles were adhered to Collodion and 2% (v / v) amyl acetate film-covered grids (300 mesh Formvar/carbon-supported copper support films, Electron Microscopy Sciences, Hatfield, USA). Viral particles were negatively stained with 2% (v / v ...
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bioRxiv - Cell Biology 2021Quote: ... were applied to carbon-coated and glow discharged (2 minutes at 7x10-1 mbar) 300-mesh copper EM grids (Electron Microscopy Sciences; CF300-CU) and incubated for four minutes at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Microbiology 2022Quote: ... thick resin sections (250 nm) were cut and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were decorated with fiducial marker and stained with Hoechst to visualize nuclear regions ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5mM Mg(OAc)2 wash buffer to an optical density of 2 A260/mL (corresponding to 40 nM of 80S) and deposited on copper grids (Electron Microscopy Sciences, CF400-CU). Grids were glow-discharged at 15 mA for 30s 4 uL of sample were incubated on the glow-discharged grids for 1min ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were washed twice for 5 min in wash buffer and fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium (Ionpath ...
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bioRxiv - Physiology 2024Quote: ... The sections were cover-slipped in Fluoromount-G™ Slide Mounting Medium with DAPI (4′,6- diamidino-2-phenylindole; Electron Microscopy Sciences, Hatfield, PA) and examined under a brightfield microscope to identify irregularities in the luminal surface in eTNAP mice ...
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bioRxiv - Neuroscience 2022Quote: Postnatal mouse were transcardially perfused with 15ml (P14) or 25ml (>P21) of 2% PFA freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Neuroscience 2021Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) in phosphate buffered saline ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was dehydrated through an ethanol series (70% ethanol, 90% ethanol, 2 x 100% ethanol; 8 minutes each) and mounted in Entellan (Electron Microscopy Science, Hatfield, PA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed for at least 2 hours (at room temperature) to overnight (at 4° C) in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in artificial sea water ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) and 2% PFA in 0.1 M in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Microbiology 2024Quote: ... This concentrate was diluted 1:2 with molecular grade water and 50 µL was fixed with 25% glutaraldehyde (stored at 4°C, Electron Microscopy Sciences, Hatfield, PA, USA) to a final concentration of 0.25% ...
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bioRxiv - Neuroscience 2024Quote: ... 70 nm ultrathin sections were taken and post-stained with 2% aqueous uranyl acetate and lead citrate (22400, 22405, 17800, 17900, 21140, Electron Microscopy Sciences, Hatfield, PA, USA). Samples were imaged on a TEM (Jeol JEM-1400 Plus ...