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Citations for Electron Microscopy Sciences :
651 - 700 of 757 citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... neurons were washed with cold PBS supplemented with 0.1 mM CaCl2 and 2 mM MgCl2 (PBSCM) and fixed in 4% paraformaldehyde (Electron Microscopy Sciences)/4% sucrose for 8 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were mounted using MS Shield Mounting Medium With 4,6-diamidino-2-phenylindole (DAPI) and DABCO™ (EMS #17989-20).
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bioRxiv - Immunology 2024Quote: ... A 2 µl droplet of samples was placed on a formvar coated 300 mesh Cu Thin-Bar grid (EMS; Hatfield, PA), coating side down ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS twice followed by fixation and permeablization in 1 ml PBS containing 2% PFA (Electron Microscopy Sciences) and 0.1% saponin (Sigma–Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The contrast of the samples was enhanced by staining with 2% uranyl acetate in 50% ethanol (Molar Chemicals, Electron Microscopy Sciences) and 2% lead citrate in distilled water (Electron Microscopy Sciences).
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... 1 mL of culture was fixed at ambient temperature in the dark (1-2 h) with paraformaldehyde 3.8% (PFA, Electron Microscopy Sciences). Cells were then centrifuged 3 min at 8000 g and washed 3 times with 500 µL PBS ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 min ...
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bioRxiv - Immunology 2024Quote: ... were mixed with 0.5 µl of 0.04 mM Lauryl maltose neopentyl glycol (LMNG) solution immediately before sample deposition onto QUANTIFOIL® 2/1 400 mesh grids (EMS), which were plasma cleaned for 10 s using Gatan Solarus 950 Plasma system (Ar/O2 gas mixture) ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were submerged in 1.5% uranyl acetate in absolute methanol at -90 °C for 2 days and infiltrated with HM20 Lowicryl resin (Electron Microscopy Sciences) over 2 days at -45 °C ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed with acetone and infiltrated with increasing concentrations (25, 50, 75 and 100%; 2 hours each) of EPON resin (Electron Microscopy Sciences). 100% EPON solution was exchanged two times in 12-hour steps ...
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bioRxiv - Biophysics 2022Quote: ... They were then kept at room temperature for ∼2 h before being applied to carbon-coated 200-hex copper grids (EMS, Hatfield PA). Freshly ionized grids were floated on a 10 ul drop of sample for 1 minute ...
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bioRxiv - Microbiology 2022Quote: ... grids were washed by contact with 2 drops of milliQ water and were negatively stained with 20 µl of 1% uranyl acetate (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed for 10 or 30 (if infected with SARS-CoV-2) min in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences).
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bioRxiv - Biophysics 2022Quote: U-2 OS cells expressing pRFPs-mito were seeded into 96-well optical polymer base microplates and fixed with 2% paraformaldehyde (Electron Microscopy Sciences) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Physiology 2020Quote: 1-2 tracheal rings were removed post-mortem and embedded in Peel-A-Way embedding molds containing Tissue-Tek OCT (Electron Microscopy Sciences). Molds were placed in a container filled with dry ice until frozen ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were fixed using 4% paraformaldehyde (Serva, Heidelberg, Germany) and 2% glutaraldehyde (Serva) in PBS followed by staining with 0.5% osmium tetroxide (EMS, Hatfield, PA, USA). After thorough rinsing in PBS ...
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bioRxiv - Genetics 2021Quote: ... 3 μl of the diluted 3C library was deposited onto a plasma-clean Quantifoil R2/2 holey carbon grid (Electron Microscopy Sciences), blotted with a filter paper for 2 seconds ...
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bioRxiv - Immunology 2021Quote: ... EM grids were prepared by staining 1:20 and 1:100 dilutions (in PBS plus 250 mM NaCl) of lyophilised and resuspended sample with 2 % uranyl formate solution on carbon-coated CF400-CU-UL grids (Electron Microscopy Sciences) as described earlier ...
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bioRxiv - Neuroscience 2020Quote: ... the slice was placed in a fixative consisting of 2% formaldehyde (diluted from 8% formaldehyde, EM grade, Electron Microscopy Sciences 157-8) and 2 % glutaraldehyde (diluted from 25% glutaraldehyde ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were freeze-substituted in a Leica AFS2 automatic freeze substitution unit at −85°C for 4 days in 2% (w/v) osmium tetroxide (Electron Microscopy Sciences) and 8% 2,2-dimethoxypropane (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Denatured RNA was refolded in the presence of 2 μM of LNA ASO by incubating the mixture in 340 mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5 mM MgCl2+ ...
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bioRxiv - Biophysics 2021Quote: ... 2uL of dialyzed sample was then applied to glow-discharged (1 min, easiGlow, Pelco) R2/2 200- or 300-mesh Quantifoil holey carbon grids (Electron Microscopy Sciences). The grids were subsequently blotted for 2 or 3 seconds ...
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bioRxiv - Biophysics 2022Quote: ... Holey gold films with ~2 μm holes were nanofabricated as described previously (Marr et al., 2014) on 300 mesh Maxtaform copper-rhodium grids (Electron Microscopy Sciences). Specimens with 10 mM ATP were prepared by first applying 0.4 μL of 50 mM ATP in buffer (70 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... the unroofed cells were placed in fresh 4% PFA (for immunofluorescence) or in 2% glutaraldehyde (for TEM, EM grade, Electron Microscopy Science) for fixation at 4C.
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bioRxiv - Neuroscience 2020Quote: ... Rbp4-Cre;Ai14;Snap25+/+ and Rbp4-Cre;Ai14;Snap25fl/fl brains at P18 were perfused with 0.1 M phosphate buffer containing 2% PFA (Electron Microscopy Sciences, 15714) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grid covered with a formvar film (FF2010-Cu, Electron Microscopy Sciences, USA). Sections were covered by 0.1 μm TetraSpeck microsphere fiducials (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: Mice were sacrificed by cervical dislocation and sciatic nerve were exposed and fixed by adding fixative solution (2% PFA (15710, Electron Microscopy Sciences), 2.5% Glutaraldehyde (16220 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were gradually returned to 175 mM PB in decreasing fructose concentrations over 16 hrs and then post-fixed in 2% GA in 150 mM sodium cacodylate buffer (CB, Electron Microscopy Sciences) for 2 hrs.
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bioRxiv - Genomics 2020Quote: Protein ICC was performed as described previously (Martin et al., 2013) on cells fixed in 4% paraformaldehyde or 2% paraformaldehyde (Electron Microscopy Sciences). For double and triple immunocytochemistry using antibodies to methylated DNA ...
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bioRxiv - Bioengineering 2020Quote: Both the 3D bioprinted and the manually prepared SK-N-BE(2) neuroblastoma spheroids were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) diluted in 1% Triton (Sigma-Aldrich ...
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bioRxiv - Pathology 2021Quote: Liver tissue samples were fixed in 0.1 M cacodylate-buffered Karnovsky fixative containing 2.5% glutaraldehyde and 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) overnight at room temperature with a subsequent post-fixation in 1% osmium tetroxide (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... of 0.01 and cultured in 6-well plates for 48 h with cMEM or cells from ETAs of COVID-19-infected patients were fixed with a freshly prepared 2% or 4% PFA solution (20% stock diluted in 1X HBSS; Electron Microscopy Sciences) or a 1.6X BD FACS™ Lysis Solution (1:6 in sterile dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were washed by dipping in two separate drops of water followed by two drops of 2% uranyl acetate (Electron Microscopy Sciences). Grids were examined at the Northwestern Electron Probe Instrumentation Center (EPIC ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... 1.8 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV using a blot time of 25-30 seconds and a blot force of 22 ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Pathology 2021Quote: ... slides were washed twice with 1 mL of PBS wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Neuroscience 2021Quote: ... was applied to glow-discharged Quantifoil holey carbon grids (Gold R2/1; 200 mesh) overlaid with an additional 2-nm carbon layer (Electron Microscopy Sciences). The grids were blotted for 3-5 s and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Genomics 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... They were carefully placed on a fine layer of resin between 2 sheets of ACLAR® embedding films (EMS, cat# 50425-25) for polymerization at 55 °C for 72 h ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Bioengineering 2022Quote: ... the cholangiocyte organoids were fixed with TEM fixative containing 2% paraformaldehyde/2.5 glutaraldehyde in 100mM cacodylate buffer (Electron Microscopy Sciences, Hartfield, PA) for 2 hours at room temperature ...