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Citations for Electron Microscopy Sciences :
651 - 700 of 942 citations for 5 Methoxypyridine 2 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; 17985-50; Electron Microscopy Sciences, Hatfield, PA, USA). Images were taken with an All-in-One Fluorescence Microscope BZ-X710 (KEYENCE Corp. ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed in freshly diluted 2% paraformaldehyde (PFA) in PBS (16% PFA purchased from EMS Cat# 15710) for 20 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2024Quote: ... 10 μL of parasite-containing media from each flask was added to 90 μL of 2% PFA (EMS) to fix and dilute the parasites at a 1:10 ratio in a 96-well flat bottom plate ...
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bioRxiv - Immunology 2024Quote: ... coated grids (R2/2, Au 200-mesh London Finder grid coated with extra thick carbon, Electron Microscopy Sciences) for another 24 hours to allow sufficient adherence ...
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bioRxiv - Immunology 2024Quote: ... 2% PFA was prepared from 16% PFA solution (Paraformaldehyde 16% Solution, EM Grade, Electron Microscopy Sciences, Hatfield, PA) in nanopure water ...
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bioRxiv - Biochemistry 2024Quote: Two μl of each FAN1-PCNA-DNA complex sample was applied to a 300-mesh copper Quantifoil R 1.2/1.3 + 2 nm holey carbon grid (EMS), which was previously glow-discharged for 90 seconds at 15 mA current using an easiGlow (PELCO) ...
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bioRxiv - Cell Biology 2024Quote: ... 2% PFA was prepared from 16% PFA solution (Paraformaldehyde 16% Solution, EM Grade, Electron Microscopy Sciences, Hatfield, PA) in nanopure water ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of the diluted DNA solution was dropped onto a carbon film coated TEM grid (Electron Microscopy Sciences, PA, USA), and incubated at room temperature for 1.5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... DE) was diluted to 5 μg/mL in assay buffer: 1X PBS with 0.1% acetylated BSA (Electron Microscopy Sciences, Hatfield, PA) in a 96-well plate and loaded onto streptavidin biosensors (FortéBio ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at the Pole Facultaire de Microscopie Electronique at the University of Geneva as described 5 except uranyl acetate staining was replaced with Reynold’s (lead citrate, Electron Microscopy Sciences), and 20 nm sections were used ...
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bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Immunology 2020Quote: ... ~ 5 μL of the sonicated samples were drop cast onto 300-mesh formvar carbon-coated copper grids (Electron Microscopy Sciences, UK) and negatively stained with UranyLess (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed 3 × 5 minutes in PBS and mounted to slides using FluoroGel with DABCO (Electron Microscopy Sciences, #17985) and 1.5 thickness coverglass (Fisherbrand ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS with 0.2% Triton X-100 and mounted onto slides with DAPI Fluoromount (#17984-24, Electron Microscopy Sciences) before visualization with an EVOS imaging system (Thermo Fisher Sci).
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on studs using silver paint and coated with 5 nm of Palladium (sputter coater EMS150TS; Electron Microscopy Sciences). Samples were imaged at 5 kV on a FEI Magellan 400 XHR Field Emission Scanning Electron Microscope at the Stanford Nano Shared Facilities.
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bioRxiv - Cancer Biology 2024Quote: ... and pellets were resuspended in a 5 ml volume of fixative master mix of Paraformaldehyde-Glutaraldehyde Solution Karnovsky’s Fixative (Electron Microscopy Sciences). Fixative solution was prepared with 16% paraformaldehyde solution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 µL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... was infused into the well of the chamber and covered with a 5-mm diameter glass coverslip (7229605, Electron Microscopy Sciences). The skin was sealed with VetbondTM Adhesive ...
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bioRxiv - Biophysics 2023Quote: ... 5 µls were applied to a glow discharged 1.2/1.3 CF-1.2/1.3-3Au-50 (Electron Microscopy Sciences, part # AUFT313-50) cryo-EM grid and allowed to absorb for 30 sec ...
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bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
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bioRxiv - Microbiology 2023Quote: ... Slides were subsequently washed three more times for 5 minutes by immersion in 0.1% Tween-20 in PBS and mounted in EMS Shield Mount with DABCO (Electron Microscopy Sciences). Imaging was performed using a Carl Zeiss LSM710 confocal microscope at the microscopy imaging center (MIC ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg of EVs was placed onto glow discharged 200-mesh formvar/carbon copper grids (Electron Microscopy Sciences, Hatfield Township, PA). The EVs were washed two times with water and then fixed in 2.5% glutaraldehyde ...
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bioRxiv - Microbiology 2024Quote: Clinical samples and biofilm samples fixed using the SEM fixation buffer were subsequently subjected to a series of washing steps (3 repetitions for 5 mins each) with 0.1M Cacodylate buffer pH 7.4(Electron Microscopy Sciences, USA) and distilled water (Gibco) ...
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bioRxiv - Plant Biology 2024Quote: Aliquots (5 µL) of resuspended P40 pellets were spotted onto Formvar and carbon-coated copper electron microscopy grids (Electron Microscopy Sciences) that were glow-discharged at 15mA for 60 seconds prior to application of samples ...
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bioRxiv - Neuroscience 2024Quote: ... and the hole in the bone was covered with a round cover glass (d = 5 mm; Electron Microscopy Sciences, 72296–05). A helicopter-shaped head plate (model 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... main inflorescences (not side branches) were cut and dissected with a Dumont tweezer (Electron Microscopy Sciences, style 5, no. 72701-D) to remove buds older than stage 9 or 10 ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed three times (650xg, 5 min, RT) with Perm Buffer before fixation in 1.6% paraformaldehyde (PFA) (Electron Microscopy Sciences) in PBS overnight with 125nM Iridium (Fluidigm ...
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bioRxiv - Bioengineering 2024Quote: ... A 5 μL drop of the solution was deposited onto a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences). The grid was incubated for 2 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... TolAQR (abs280=5) or TonB-ExbBD (abs280=1.4) were applied to a Quantifoil R1.2/1.3 300 mesh grid (Electron Microscopy Sciences, Protochips, Inc.) that had been glow discharged for 45 s at 15 mA (Pelco easiGlow ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µl of protein complex was deposited on a holey copper grid (QUANTIFOIL R1.2/1.3, 200 mesh, EMS cat# Q250-CR1.3) or a carbon film copper grid (QUANTIFOIL R 1.2/1.3 ...
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bioRxiv - Developmental Biology 2021Quote: ... whole-mount preparations of the lateral wall of lateral ventricles of four animals per group were dissected and fixed with 2 % PFA + 2.5 % glutaraldehyde (EMS) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Pathology 2022Quote: ... perfused in toto (PBS; 10mL) and fixed (2% formaldehyde EMS #15710, 2.5% glutaraldehyde EMS #16220 in 0.1M Phosphate Buffer). Kidneys were harvested and fixed in the same solution ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with CyFACS and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS (Rockland) ...
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bioRxiv - Biophysics 2021Quote: ... and permeabilized for 2 min with 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and Thermo Fisher, 28314) in pre-warmed (37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and rinsed in distilled water prior to en bloc staining with 2% uranyl acetate (22400, Electron Microscopy Sciences, USA). After dehydration through a graded ethanol series ...
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bioRxiv - Neuroscience 2021Quote: One 5 mm sample from each naïve nerve was fixed for 48 hours at 4° C in a solution of 2% glutaraldehyde (16216, EMS), 3% paraformalydehyde (15754-S ...
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bioRxiv - Biochemistry 2021Quote: ... Ycf1 sample was applied to a CFlat-1.2/1.3-4C-T (WT Ycf1) or QF-1.2/1.3-4Au grid (E1435Q Ycf1) purchased from Electron Microscopy Sciences. Grids were placed inside of a Leica EM GP2 equilibrated to 10°C and 80% humidity ...
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bioRxiv - Neuroscience 2020Quote: ... Brain slices were washed extensively in cacodylate buffer at room temperature and stained sequentially with 2% osmium tetroxide (EMS) in cacodylate buffer ...
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bioRxiv - Microbiology 2021Quote: ... the grids were washed with PBS three times before being fixed with 2% EM grade paraformaldehyde (EMS, EM Grade) for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... infected and uninfected cells were fixed by rapidly transferring Aclar slabs into Karnovsky’s fixative (2% Glutaraldehyde (EMS cat.#16000) and 4% PFA (EMS cat.#15700 ...
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bioRxiv - Molecular Biology 2021Quote: Eyes were fixed in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed and then fixed in FACS buffer supplemented with 2% PFA (15710-S, Electron Microscopy Sciences) for 15 minutes at room temperature ...