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Citations for Electron Microscopy Sciences :
851 - 900 of 942 citations for 5 Methoxypyridine 2 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... 3 μl of the diluted 3C library was deposited onto a plasma-clean Quantifoil R2/2 holey carbon grid (Electron Microscopy Sciences), blotted with a filter paper for 2 seconds ...
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bioRxiv - Immunology 2021Quote: ... EM grids were prepared by staining 1:20 and 1:100 dilutions (in PBS plus 250 mM NaCl) of lyophilised and resuspended sample with 2 % uranyl formate solution on carbon-coated CF400-CU-UL grids (Electron Microscopy Sciences) as described earlier ...
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bioRxiv - Neuroscience 2020Quote: ... the slice was placed in a fixative consisting of 2% formaldehyde (diluted from 8% formaldehyde, EM grade, Electron Microscopy Sciences 157-8) and 2 % glutaraldehyde (diluted from 25% glutaraldehyde ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were freeze-substituted in a Leica AFS2 automatic freeze substitution unit at −85°C for 4 days in 2% (w/v) osmium tetroxide (Electron Microscopy Sciences) and 8% 2,2-dimethoxypropane (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Denatured RNA was refolded in the presence of 2 μM of LNA ASO by incubating the mixture in 340 mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5 mM MgCl2+ ...
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bioRxiv - Biophysics 2021Quote: ... 2uL of dialyzed sample was then applied to glow-discharged (1 min, easiGlow, Pelco) R2/2 200- or 300-mesh Quantifoil holey carbon grids (Electron Microscopy Sciences). The grids were subsequently blotted for 2 or 3 seconds ...
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bioRxiv - Biophysics 2022Quote: ... Holey gold films with ~2 μm holes were nanofabricated as described previously (Marr et al., 2014) on 300 mesh Maxtaform copper-rhodium grids (Electron Microscopy Sciences). Specimens with 10 mM ATP were prepared by first applying 0.4 μL of 50 mM ATP in buffer (70 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... the unroofed cells were placed in fresh 4% PFA (for immunofluorescence) or in 2% glutaraldehyde (for TEM, EM grade, Electron Microscopy Science) for fixation at 4C.
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bioRxiv - Neuroscience 2020Quote: ... Rbp4-Cre;Ai14;Snap25+/+ and Rbp4-Cre;Ai14;Snap25fl/fl brains at P18 were perfused with 0.1 M phosphate buffer containing 2% PFA (Electron Microscopy Sciences, 15714) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grid covered with a formvar film (FF2010-Cu, Electron Microscopy Sciences, USA). Sections were covered by 0.1 μm TetraSpeck microsphere fiducials (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: Mice were sacrificed by cervical dislocation and sciatic nerve were exposed and fixed by adding fixative solution (2% PFA (15710, Electron Microscopy Sciences), 2.5% Glutaraldehyde (16220 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were gradually returned to 175 mM PB in decreasing fructose concentrations over 16 hrs and then post-fixed in 2% GA in 150 mM sodium cacodylate buffer (CB, Electron Microscopy Sciences) for 2 hrs.
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bioRxiv - Genomics 2020Quote: Protein ICC was performed as described previously (Martin et al., 2013) on cells fixed in 4% paraformaldehyde or 2% paraformaldehyde (Electron Microscopy Sciences). For double and triple immunocytochemistry using antibodies to methylated DNA ...
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bioRxiv - Bioengineering 2020Quote: Both the 3D bioprinted and the manually prepared SK-N-BE(2) neuroblastoma spheroids were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) diluted in 1% Triton (Sigma-Aldrich ...
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bioRxiv - Pathology 2021Quote: Liver tissue samples were fixed in 0.1 M cacodylate-buffered Karnovsky fixative containing 2.5% glutaraldehyde and 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) overnight at room temperature with a subsequent post-fixation in 1% osmium tetroxide (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... of 0.01 and cultured in 6-well plates for 48 h with cMEM or cells from ETAs of COVID-19-infected patients were fixed with a freshly prepared 2% or 4% PFA solution (20% stock diluted in 1X HBSS; Electron Microscopy Sciences) or a 1.6X BD FACS™ Lysis Solution (1:6 in sterile dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were washed by dipping in two separate drops of water followed by two drops of 2% uranyl acetate (Electron Microscopy Sciences). Grids were examined at the Northwestern Electron Probe Instrumentation Center (EPIC ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... 1.8 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV using a blot time of 25-30 seconds and a blot force of 22 ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Pathology 2021Quote: ... slides were washed twice with 1 mL of PBS wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Molecular Biology 2021Quote: 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Neuroscience 2021Quote: ... was applied to glow-discharged Quantifoil holey carbon grids (Gold R2/1; 200 mesh) overlaid with an additional 2-nm carbon layer (Electron Microscopy Sciences). The grids were blotted for 3-5 s and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Genomics 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... They were carefully placed on a fine layer of resin between 2 sheets of ACLAR® embedding films (EMS, cat# 50425-25) for polymerization at 55 °C for 72 h ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Bioengineering 2022Quote: ... the cholangiocyte organoids were fixed with TEM fixative containing 2% paraformaldehyde/2.5 glutaraldehyde in 100mM cacodylate buffer (Electron Microscopy Sciences, Hartfield, PA) for 2 hours at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... These cells in the log-growth phase were collected and deposited on glow-discharged Quantifoil grids (R 2/1, Cu 200-mesh grid, Electron Microscopy Sciences), as described previously (5) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were first fixed with 1 mL of the fixative (2% paraformaldehyde [Electron Microscopy Sciences] dissolved in 250 mM HEPES [pH 7.4]) for 10 min at room temperature (approximately 25°C) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by ice-cold EM fixative (1.25% glutaraldehyde [Electron Microscopy Sciences, 16220], 2% paraformaldehyde [Electron Microscopy Sciences, 19210], 0.1M sodium cacodylate buffer). Brains were removed and stored for 8 days in EM fixative at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were then treated with a nitrocellulose solution (1:1 solution of sterile Collodion in 2% amyl acetate and amyl acetate; Electron Microscopy Sciences) and then left to airdry for one hour ...
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bioRxiv - Microbiology 2023Quote: ... About 4 µl of aerated and hypoxia samples were pipetted on glow-discharged Quantifoil Au extra thick EM-grids (R2/2, 200 mesh, Electron Microscopy Sciences). The extra fluid was blotted (blot force of 7 s and blot time 5 sec ...
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bioRxiv - Microbiology 2023Quote: 3.5 µL of Vipp1 at a final concentration of 60 µM was incubated for 90 seconds on a plasma cleaned holey R2/2 Quantifoil copper grid (Electron Microscopy Science) before plunge freezing in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed with pre-warmed (37°C) PBS and permeabilized for 2 minutes using 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37 °C ...
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bioRxiv - Plant Biology 2024Quote: Leaf tissue was collected from the interveinal region of the youngest fully expanded leaves and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, EMS) and 2.5 % paraformaldehyde (Ted Pella Inc) ...
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bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and coverslips were mounted over slides in Fluoro-Gel (EMS; 17985-10) and imaged by spinning disc confocal microscopy and Aperio Versa 8 fluorescent slide scanner.
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bioRxiv - Biochemistry 2023Quote: ... Resuspended culture was mixed with 10 nm gold beads as fiducial markers and then applied onto glow-discharged copper R2/2 200 mesh Quantifoil holey carbon grids (Electron Microscopy Sciences) in Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... One end of the tube was clamped with a hemostat and the tube was filled with room temperature 2% paraformaldehyde (PFA) (Electron Microscopy Sciences) with a syringe and metal cannula ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...
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bioRxiv - Cell Biology 2024Quote: Media were formulated as previously described [2] to create disease progression from Normal Fasting (NF) to Early Metabolic Syndrome (EMS or MASLD) and Late Metabolic Syndrome (LMS or late stage MASLD) ...
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bioRxiv - Biophysics 2020Quote: ... 5 uL of DNA origami solution (5 nM) was deposited onto fresh glow discharged carbon film with copper grids (CF200H-CU; Electron Microscopy Sciences Inc., Hatfeld, PA), and the sample was then allowed to absorb onto the surface for 30s ...
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bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 7 and 8) or DNA stained with Hoechst 33258 and mounted on glass slides using Fluoromount G (Electron Microscopy Sciences; Figs. 5, 6E, 6G, S1). APEX enzymes and YFPSMO were detected by GFP and YFP fluorescence ...
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bioRxiv - Cell Biology 2022Quote: ... stained en bloc with aqueous 1.0% uranyl acetate for 1 h on ice, (5) progressively dehydrated through an ethanol series (50%, 70%, 90%, and 100%) and 100% propylene oxide (Electron Microscopy Sciences, Hatfield, PA, USA) each for 10 min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were placed on TEM one-slot grids (Agar Scientific, France) coated with Formvar film and stained 20 minutes with 5% uranyl acetate (Electron Microscopy Science, Hatfield, PA, USA) and 5 minutes Reynolds lead citrate ...