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Citations for Electron Microscopy Sciences :
551 - 586 of 586 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The proteins were diluted to 0.01 mg/mL and 4 μL was applied to a glow discharged Formvar/Carbon grid (Electron Microscopy Sciences) for 30 s ...
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bioRxiv - Neuroscience 2023Quote: Animals were deeply anesthetized with ketamine/xylazine and transcardially perfused with 5-10 mls of 37°C 0.9% sterile saline followed by 10 mls of room temperature 4% EM Grade paraformaldehyde (PFA, Electron Microscopy Sciences) in 0.9% saline.
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of spike-protein at 0.03 mg/ml in 1X TBS was deposited on a glow-discharged carbon coated copper mesh grid (Electron Microscopy Sciences) and blotted off ...
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bioRxiv - Molecular Biology 2020Quote: 3 µL of NT-NTR1-Gαi1β1γ1-cND complex at a concentration of 0.02 mg/mL was applied onto a glow-discharged continuous carbon grid (Electron Microscopy Sciences, Inc.). After two minutes of adsorption ...
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bioRxiv - Immunology 2019Quote: ... Eight to 10 ml of blood was gently fixed and lysed by incubating with 792 µl of 16% paraformaldehyde (Electron Microscopy Sciences, Hatfield ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Neuroscience 2021Quote: ... The animals were intracardially perfused with 100 mL of 0.9% physiological saline followed by approximately 250 mL of freshly prepared 0.1 M sodium cacodylate buffer (pH 7.4; Electron Microscopy Sciences cat #11653), which contained 2.5% glutaraldehyde (Electron Microscopy Sciences cat #16320 ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Microbiology 2020Quote: Complexes of M4H2K1 Fab and BG505 UFO.664 trimer were purified by SEC to remove unbound Fab and diluted to 0.01 mg/mL in Tris-buffered saline prior to adsorption onto carbon-coated and plasma cleaned copper mesh grids (Cu400, Electron Microscopy Sciences). Grids were stained with 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were incubated at a molar ratio of 4 Fab:3 spike monomer for ∼1 h and approximately 3 µL of the sample at concentrations of about 10 to 15 µg/mL was applied to a glow discharged grid with continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences). The grids were stained with 0.75% uranyl formate (UF ...
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bioRxiv - Immunology 2020Quote: ... 10 mM EDTA) to a final concentration of ∼50-200 µg/ml and immediately applied to the glow-discharged grid (Electron Microscopy Sciences). After 30 seconds ...
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bioRxiv - Neuroscience 2021Quote: ... to be non-responsive to toe pinch and transcardially perfused with 10 ml of 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences (EMS)) to flush the vasculature followed by buffered fixative made of 2.5% glutaraldehyde (EMS) ...
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bioRxiv - Microbiology 2021Quote: 8 μl of 1 x 1010 pfu/mL of phages was applied to a copper mesh grid coated with formvar and carbon (Electron Microscopy Sciences) for 2 minutes and then gently blotted off with a piece of Whatman filter paper ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified virions at 1 × 1010 PFU mL-1 was applied to a 400-mesh C-flat holey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Neuroscience 2023Quote: ... all animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed with PBS and fixed in 1 mL ice cold 4% PFA (Electron Microscopy Science, Hatfield, PA, USA) diluted in PBS ...
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bioRxiv - Molecular Biology 2023Quote: 5 μl of 0.02 mg/ml purified Nsp15 sample was applied onto glow-discharged ultrathin formvar/carbon supported copper 400-mesh grids (Electron Microscopy Sciences), blotted and stained with 2.0% uranyl acetate ...
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bioRxiv - Neuroscience 2023Quote: ... animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Biophysics 2024Quote: 3 µL diluted MgtA (0.01 mg/mL) was deposited on a carbon-coated 400 mesh copper grid (CF400-CU, EMS, Hatfield, PA, USA) following glow discharging (PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Cell Biology 2024Quote: ... cells were first fixed with 1 mL of the fixative (2% paraformaldehyde [Electron Microscopy Sciences] dissolved in 250 mM HEPES [pH 7.4]) for 10 min at room temperature (approximately 25°C) ...
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bioRxiv - Neuroscience 2020Quote: ... the appropriate plane of anesthesia was checked by a lack of toe pinch reflex and the animals were transcardially perfused with 15 ml 0.15 M cacodylate buffer (EMS, Hatfield, PA, pH 7.4) followed by 30 ml fixative mixture containing 0.08 M cacodylate (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 20,000 seeds homozygous for the SCRpro::GFP-SCR transgene were treated overnight at room temperature with 10 mL 0.1% ethyl methanesulfonate (EMS, Sigma M0880, MO, USA). After thorough washing ...
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bioRxiv - Neuroscience 2021Quote: ... the appropriate plane of anesthesia was checked by a lack of toe pinch reflex and the animals were transcardially perfused with 15 ml 0.15 M cacodylate buffer (EMS, Hatfield, PA, pH 7.4) followed by 30 ml fixative mixture containing 0.08 M cacodylate (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... 3 mL of cells was harvested by centrifugation for 3 min at 1500 × g at room temperature and fixed for 30 min in 1 mL of 2.5% (v/v) glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) in 0.1 M cacodylate buffer (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Fibril assemblies were next analyzed via transmission electron microscopy at an applied concentration of 0.4 mg per mL, with fibrils absorbed to glow-discharged 300 mesh (Ladd Research Industries, Inc.) and stained with 2% uranyl acetate (Electron Microscopy Sciences, Inc.) in water ...
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bioRxiv - Plant Biology 2022Quote: ... without mPrep screens and held in mPrep CPD Holder (Microscopy Innovations, Cat # 2250) that was then placed in a 50 ml PTFE beaker (EMS Diasum, Cat# 60942) containing LN2 frozen substitution fluid (2% osmium tetroxide in acetone with 3% water ...
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bioRxiv - Neuroscience 2019Quote: ... NaNO2 (# S25560) and NaCl (# 7647-14-5) which is followed by perfusion with 40 mL of cold 4% paraformaldehyde (Electron Microscopy Sciences, #RT-15714) in 1X-phosphate buffered saline (PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μl of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with FACS buffer and once with PBS before fixing with 200 mL 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Cat# 15710) in PBS overnight or longer ...
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bioRxiv - Biochemistry 2023Quote: ... 2.5mM Mg(OAc)2 wash buffer to an optical density of 2 A260/mL (corresponding to 40 nM of 80S) and deposited on copper grids (Electron Microscopy Sciences, CF400-CU). Grids were glow-discharged at 15 mA for 30s 4 uL of sample were incubated on the glow-discharged grids for 1min ...
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bioRxiv - Biochemistry 2024Quote: Purified receptor was diluted to ∼5 ug mL−1 and applied to freshly glow-discharged EM carbon coated copper grid (Electron Microscopy Sciences, CF400-Cu,) for 30 sec ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL of high titre phage stock (~109 PFU/mL) was loaded onto plasma-glowed carbon coated 300 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA, USA). After 60 s the excess specimen was removed by blotting and 10 μL of 1% w/v phosphotungstic acid (PTA ...
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bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
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bioRxiv - Genetics 2019Quote: ... 4 µL of sample at a final concentration of 0.01 mg/mL for high salt conditions or 0.1 mg/mL for low salt conditions were absorbed for 30 seconds on a glow-discharged carbon coated grid (Electron Microscopy Sciences, ref CF300-CU). The grids were then negatively stained for 1 minute using 2% uranyl formate ...