Labshake search
Citations for Electron Microscopy Sciences :
501 - 550 of 586 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: Purified NP at 0.01 mg/mL was applied to glow-discharged carbon-coated copper grids (Electron Microscopy Sciences) and stained with uranyl acetate immediately before imaging ...
-
bioRxiv - Immunology 2023Quote: ... complexes were diluted to 0.01 mg/mL and immediately applied to EM grids (Electron Microscopy Sciences #CF200-Cu). Grids were then incubated for one minute ...
-
bioRxiv - Molecular Biology 2024Quote: ... 3 μl of the purified G6PDH-OpcA sample at a protein concentration of approximately 1 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and directly plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... Temporal bones were immediately removed and fixed via perfusion of 1 ml cold 4% PFA (Electron Microscopy Science) in 1X PBS through the oval and round windows ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by adding ddH2O up to a final volume of 30 mL after accounting for glutaraldehyde (Electron Microscopy Science) volume for final BSA concentrations equal to ...
-
bioRxiv - Cell Biology 2019Quote: ... homozygous pans1-1 seeds were incubated for 17 h at room temperature in 5 mL of 0.3% (v/v) ethyl methanesulfonate (EMS) (SIGMA ...
-
bioRxiv - Plant Biology 2021Quote: ... and resuspended either in 0.3 mL Milli-Q water for vitrification or 4 % para-formaldehyde (EM grade #15710 from EMS) in 1x PBS for chemical fixation ...
-
bioRxiv - Physiology 2019Quote: ... hPCLS were washed with PBS (3X 1ml) and fixed in 1 ml of 4% PFA (EMS EM grade#5710) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cells were then resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences, cat. #15714S, diluted in PBS) and incubated at 4°C for 15 minutes with rocking ...
-
bioRxiv - Bioengineering 2020Quote: ... Purified complexes were deposited at approximately 0.003 mg/ml onto carbon-coated copper grids (EMS, Hatfield, PA, United States) for 2 mins ...
-
bioRxiv - Biophysics 2023Quote: 3 µL of MRS2 (0.02 mg/mL) was applied on a glow discharged carbon-coated 400 square mesh copper grid (EMS) and incubate for 1 min ...
-
bioRxiv - Immunology 2023Quote: 3 μL sample at 1.8 mg/ml was applied to a glow-discharged Quantifoil R1.2/1.3 400 mesh gold grid (Electron Microscopy Sciences), blotted for 4 s in 100% humidity at 4 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2023Quote: ... and resuspended either in 0.3 mL Milli-Q water for vitrification or 4 % para-formaldehyde (EM grade #15710 from EMS) in 1x PBS for chemical fixation ...
-
bioRxiv - Plant Biology 2024Quote: ... mix well and adjust pH with 9 mL 1N NaOH (Electron Microscopy Sciences, Cat. No. 21160, Hatfield, PA, USA). This solution was filtered with a 0.2 µm PES filter prior to use.
-
bioRxiv - Genetics 2023Quote: ... were imbibed overnight in water at 4 °C and then incubated for 12 h in 50 mL of 0.25% ethyl methane sulfonate (EMS). The EMS was subsequently washed several times with water ...
-
bioRxiv - Molecular Biology 2020Quote: ... we spread 5×104 protoplasts onto a regeneration plate and subjected them to a treatment of either 6 sec of 15 W UV or 12 μg/ml ethyl methanesulfonate (EMS) to cause random mutations in the genomes ...
-
bioRxiv - Microbiology 2021Quote: ... Nanoparticle samples were diluted to ∼0.02 mg/ml and added onto carbon-coated copper 400 mesh grids (Electron Microscopy Sciences) that had been plasma cleaned for 10 s with Ar/O2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3 μL of NT-NTR1-Gαi1β1γ1-cND at a concentration between 1.5 mg/mL to 1.7 mg/mL was applied onto glow discharged C-flat holy carbon grids (R1.2/1.3, 400 mesh copper, Electron Microscopy Sciences) or Quantifoil holy carbon grids (R1.2/1.3 ...
-
bioRxiv - Biophysics 2022Quote: ... A 3.5 μL sample containing the complex (1 mg/ml) was applied to a glow-discharged UltraAuFoil grid (R1.2/1.3, mesh 300) (Electron Microscopy Sciences), and then blotted and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
-
bioRxiv - Biochemistry 2020Quote: Three microliters of the Ric-8A:ΔN31Gαi1:4Nb complex at 0.4 mg/ml with 0.01% NP40 were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (Electron Microscopy Sciences). Grids were blotted for 4 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: Negative stain specimens were prepared by applying 4 µL of protein solution (0.1 mg/mL + 1 mM AMP-PnP) onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS). After 30 s the protein solution was blotted with a piece of filter paper ...
-
bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: Cells were seeded at 0,2 x 106 cells/mL in 24-well plates containing 12 mm round coverslips (#1.5 mm thickness, Electron Microscopy Science) with 1 mL per well and stimulated as described above ...
-
bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.05 mg/mL) were applied on the carbon side of glow-discharged carbon-coated holey copper grids (EMS CF300-CU). After 1 min ...
-
bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
-
bioRxiv - Microbiology 2023Quote: ... pelleted and washed with PBS before being resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences 30525-89-4) + 0.0075% glutaraldehyde (Sigma G6257 ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
-
bioRxiv - Immunology 2023Quote: 3.5 µl of 70 µg/mL nanoparticles were applied to glow-discharged 400-mesh carbon-coated grids (Electron Microscopy Sciences) and stained with 2% (wt/vol ...
-
bioRxiv - Genetics 2023Quote: The two epididymides of mature males were recovered in 1 mL of 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) and the sperm were allowed to swim for 15 min at 37 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.1 ml was deposited on a glass slide and covered with a 5mm coverslip (cat. # 72296-05, Electron Microscopy Sciences). 11 images of each reaction were taken ...
-
bioRxiv - Cancer Biology 2020Quote: ... DE) was diluted to 5 μg/mL in assay buffer: 1X PBS with 0.1% acetylated BSA (Electron Microscopy Sciences, Hatfield, PA) in a 96-well plate and loaded onto streptavidin biosensors (FortéBio ...
-
bioRxiv - Biophysics 2020Quote: ... 3 μl microliters of freshly purified complexes (0.01 mg/ml) were deposited onto freshly glow-discharged carbon-coated 400 mesh copper electron microscopy (EM) grids (Electron Microscopy Sciences) and stained using 2% (w/v ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
-
bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
-
bioRxiv - Neuroscience 2021Quote: ... to be non-responsive to toe pinch and transcardially perfused with 10 ml of 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences (EMS)) to flush the vasculature followed by buffered fixative made of 2.5% glutaraldehyde (EMS) ...
-
bioRxiv - Biochemistry 2021Quote: 100.106 haploid HAP1 cells at 60 % confluency were treated for 72 h with 0.3 mg/mL ethyl methanesulfonate (EMS, Sigma-Aldrich) directly added to the cell medium ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 ul chromatin samples with a concentration of about 0.2 mg/ml DNA were applied to Quantifoil R2/2 200 mesh copper grids (EMS Q250-CR2). Vitrification was conducted by plunging into liquid ethane using our FEI Vitrobot Mk IV Grid Plunging System at 100% humidity ...
-
bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
-
bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 µl of DELE1 samples at 0.1 mg/ml were applied onto carbon coated 400 A° mesh Cu-Rh maxtaform grids (Electron Microscopy Sciences) which were plasma cleaned for 15 s using Pelco glow discharge cleaning system (Ted Pella ...
-
bioRxiv - Biochemistry 2023Quote: ... and 0.5 mg/mL) were incubated for 60 seconds on the carbon face of 200 mesh copper grids (Electron Microscopy Sciences) following their glow discharge at 25 mA for 30 seconds ...
-
bioRxiv - Microbiology 2023Quote: ... 3 μL of purified proteins or immune complexes (∼0.02 mg/mL) were applied onto glow discharged (20 mA for 30s) carbon-coated 400 mesh copper grids (Electron Microscopy Sciences). After 5 s ...
-
bioRxiv - Biophysics 2023Quote: ... one quarter of the bell from each medusae was chemically fixed in 10 mL of 2.5% [v/v] glutaraldehyde (Electron Microscopy Sciences), 4% [v/v] paraformaldehyde (Electron Microscopy Sciences) ...
-
bioRxiv - Biochemistry 2023Quote: ... a 4 μl aliquot of the sample (diluted concentration: 0.25 mg/ml) was placed on ultra-thin carbon film grids (CF-200-Cu-UL, Electron Microscopy Sciences, Hatfield ...
-
bioRxiv - Microbiology 2023Quote: Mice lungs were perfused with 8 mL PBS and inflated with 0.7 mL of OCT compound (Tissue-Tek) mixed 1:1 v/v with 4% paraformaldehyde (Electron Microscopy Sciences) diluted in PBS ...
-
bioRxiv - Biochemistry 2023Quote: 3 μl of dAK (1 mg/ml) was applied to QUANTIFOIL Cu R300 1.2/1.3 (Electron Microscopy Sciences, catalog no. Q3100CR1.3) that had been glow discharged using a PELCO easiGlow device (Ted Pella Inc. ...
-
bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...