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Citations for Electron Microscopy Sciences :
551 - 600 of 1382 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... they were fixed for 90 min at room temperature with 4% PFA/2% Sucrose solution supplemented with 0.1% Glutaraldehyde (GA, Electron Microscopy Sciences), washed five times with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... mexicana were fixed directly in medium for 10 min at room temperature in 2.5% glutaraldehyde (glutaraldehyde 25% stock solution, EM grade, Electron Microscopy Sciences). Centrifugation was carried out at room temperature for 5 min at 16,000 g ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...
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bioRxiv - Immunology 2024Quote: ... Sections were fixed in 10% formalin for 30 minutes at room temperature and incubated in Bouin’s fixative solution (Electron Microscopy Sciences) overnight at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... A 5 μL drop of the solution was deposited onto a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences). The grid was incubated for 2 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... A small drop (2.5 mL) of the protein solution was applied to the carbon side of a glow-discharged grid (EMS, CF300-CU) and left on the grid for 1 min ...
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bioRxiv - Biophysics 2024Quote: ... grids containing sections were incubated (face down) on 100 μL drops of Aurion Blocking Solution (Electron Microscopy Sciences, Hatfield, PA, USA) for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... was performed by placing 5 μL drops of the bacteria suspension for 3 minutes directly on glow discharged carbon coated grids (EMS). The grids were then washed with two drops of 2% aqueous uranyl acetate ...
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bioRxiv - Bioengineering 2021Quote: ... the 3D printed constructs were kept in culture for 3 weeks before fixing using paraformaldehyde (4%, Electron Microscopy Sciences, USA) for 45 minutes at RT ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Microbiology 2020Quote: ... 3 µL aliquots of purified ex vivo Enas were deposited on Formvr/Carbon grids (400 Mesh, Cu; Electron Microscopy Sciences), washed with 20 μL 1x PBS ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Biophysics 2023Quote: U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
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bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 μl was applied to a glow discharged (PELCO easiGlow) carbon-coated 300 mesh copper grid (Electron Microscopy Sciences). Grids were stained with 2% uranyl acetate and allowed to dry ...
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bioRxiv - Genomics 2024Quote: Liver tissue sections (3-5 mm thickness) were fixed by submerging in fresh 4% v/v paraformaldehyde (PFA; EMS 15710) in 1X Phosphate Buffered Saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed three times in sterile PBS and fixed in 3% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) and fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) then fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 ul aliquots of the mixture were applied to UltrAu foil R1.2/1.3 gold 300-mesh grids (Electron Microscopy Sciences). Grids were then blotted and vitrified in liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Biophysics 2024Quote: ... were diluted to[9 mg/ml and aliquots (3[μl) were applied to GF-0.6/1.0 300 Au-flat (Electron Microscopy Sciences). The cryo-EM grids were prepared using Thermo Fisher Vitrobot Mark IV at 8[°C and 100% humidity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then infiltrated for 1 h with 1:1 PO:Embed 812 (EMS), and then overnight in 1:2 PO:Embed 812 ...
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bioRxiv - Immunology 2021Quote: ... EM grids were prepared by staining 1:20 and 1:100 dilutions (in PBS plus 250 mM NaCl) of lyophilised and resuspended sample with 2 % uranyl formate solution on carbon-coated CF400-CU-UL grids (Electron Microscopy Sciences) as described earlier ...
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bioRxiv - Cell Biology 2022Quote: ... then fixed for 10 min at room temperature with pre-warmed 4% Paraformaldehyde solution in DPBS (37°C; prepared from 16% PFA (Electron Microscopy Sciences)) ...
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bioRxiv - Cell Biology 2021Quote: Mice were sacrificed by cervical dislocation and sciatic nerve were exposed and fixed by adding fixative solution (2% PFA (15710, Electron Microscopy Sciences), 2.5% Glutaraldehyde (16220 ...
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bioRxiv - Bioengineering 2020Quote: ... 10 μl of the particle solutions were dropped on the 200 mesh copper grid covered with a carbon-stabilized Formvar film (Electron Microscopy Sciences). The residual solution was removed after 5 min ...
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bioRxiv - Microbiology 2021Quote: ... of 0.01 and cultured in 6-well plates for 48 h with cMEM or cells from ETAs of COVID-19-infected patients were fixed with a freshly prepared 2% or 4% PFA solution (20% stock diluted in 1X HBSS; Electron Microscopy Sciences) or a 1.6X BD FACS™ Lysis Solution (1:6 in sterile dH2O ...
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bioRxiv - Physiology 2021Quote: ... whole heads or enucleated eyes were fixed for 20 minutes on ice in 4% PFA prepared in PBS from 20% stock solution (15713, Electron Microscopy Sciences). The samples were rinsed with PBS and stored in 100% methanol at −20°C ...
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bioRxiv - Neuroscience 2021Quote: ... the patch pipette was gently removed from the recorded PC and the slice was immediately transferred from the recording chamber to a fixation solution composed of 4% paraformaldehyde (Electron Microscopy Sciences) in ACSF for a maximum of 24h (4°C).
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Bioengineering 2024Quote: 10 μL of samples with an optimized concentration (1.0∼1.5 x 1010 /100 μL range) in double-filtered PBS solution were deposited onto the TEM grid (Formvar/Carbon 300 Mesh, Copper; #FCF300-CU, Electron Microscopy Sciences) and incubated for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... stained with Hoechst dye according to the manufacturer’s specifications and then overlayed with CitiFluor Mountant AF1 Solution (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Developmental Biology 2023Quote: For wholemount immunofluorescent labeling heads were bisected and brains removed prior to immersion fixation of the intact inner ear and skull in 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences) prepared in 67mM Sorensons phosphate buffer (pH 7.4) ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... and sections were heat-mounted to glass slides and stained with a diluted toluidine blue solution (catalog no. 14950, Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...
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bioRxiv - Neuroscience 2024Quote: ... Labella were dissected from 6—8 day-old flies and fixed in a solution containing 4% paraformaldehyde (Electron Microscopy Sciences, Cat # 15710) and 0.2% Triton X-100 for 15 minutes at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... egg-PC/ Sphingomyelin (3:1 molar ratio)} solutions were applied to a thin film of carbon-coated copper grids (Carbon film 300 mesh, Copper, Electron Microscopy Sciences) at room temperature ...
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bioRxiv - Pathology 2024Quote: ... then incubated overnight at room temperature in LFB solution (0.1% in 95% alcohol with acetic acid, Electron Microscopy Sciences, Hatfield, PA, USA). In groups of 3 or 4 with at least one normal sample ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of diluted phage solution was applied onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA, USA). Following a 3-minute incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... The leg fragments were then placed directly into 500 µl of 4% paraformaldehyde (20% EM grade stock solution, Electron Microscopy Sciences #15713) in filtered artificial seawater for 50 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...