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Citations for Electron Microscopy Sciences :
501 - 550 of 1382 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 300 mesh formvar-carbon-coated copper grids and uranyl acetate replacement solution were purchased from Electron Microscopy Sciences (Hatfeild, PA).
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bioRxiv - Microbiology 2021Quote: ... 3.5 µl of the complex solution were deposited onto a C-flat 1.2/1.3-3C holey carbon copper grid (Electron Microscopy Sciences) that had been freshly glow-discharged for 1 min at 20 mA using a PELCO easiGLOW (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ul of the experimental solutions were incubated on glow-discharged to carbon-coated copper EM grids (300 mesh, EMS). Filter paper was used to remove any excess solution and the EM samples were then washed three times with swelling buffer ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of parasite culture at 5% parasitemia was washed with PBS and re-suspended in a fresh fixative solution (4% Paraformaldehyde (EMS) and 0.0075% glutaraldehyde (EMS ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... 3 μl of solution was applied to a plasma-cleaned C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica EM GP plunge freezer operating at 25 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then placed in two serial washes of PBS solution with gentle agitation for 30 seconds each prior to being submerged for 2 hours in 2% osmium solution (RT 19172, EMS). Thereafter ...
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bioRxiv - Immunology 2021Quote: ... to a final concentration of 0.005 mM and 4 μL of this solution was applied to plasma-cleaned 1.2/1.3 C-Flat holey carbon grids (Electron Microscopy Sciences) using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
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bioRxiv - Immunology 2021Quote: ... The cells were further washed twice with 1x permeabilization buffer (eBioscience) before fixation in 1.6% PFA solution (Electron Microscopy Sciences) containing 125 nM Iridium (Fluidigm ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed with 1X PBS and fixed in 4% paraformaldehyde (PFA) solution (diluted from 16% PFA, Electron Microscopy Sciences) in PBS for 15 min ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: For collagen staining deparaffinized and re-hydrated slides were stained for 5 min in Van Gieson solution (EMS, 26374-06) followed by dehydration by 2 changes in 100% ethanol and cleared by 2 changes of xylenes ...
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bioRxiv - Cell Biology 2021Quote: ... 4 μL solution in each reaction condition was immediately transferred to a glow-discharged continuous carbon grid (Electron Microscopy Sciences) for 15 seconds before blotting and staining ...
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bioRxiv - Bioengineering 2022Quote: Microtissues were transferred to wells with 4% PFA in PBS (diluted from 32% PFA solution, Electron Microscopy Sciences 15714-S) for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were mounted in the same solution on glass slides equipped with Coverwell Imaging Chambers (Electron Microscopy Sciences, USA).
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bioRxiv - Plant Biology 2022Quote: ... with the following protocol: Dehydration and fixation in a solution containing a mixture of osmium tetroxide 0.5% (EMS, Hatfield, PA) with glutaraldehyde 0.5% (EMS ...
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bioRxiv - Neuroscience 2022Quote: ... Several FA/GA-fix tissue sections were post-fixed with 0.01% osmium tetroxide (4% osmium solution; EMS, catalog no. 19150) in 1× PBS for 1h at RT (FA/GA/OsO4-fix) ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μl of the mixed solution was dropped onto the 200 mesh formvar/carbon copper grid (Electron Microscopy Sciences, USA), allowing it to almost dry for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... grids were incubated in a solution of 10-nm diameter colloidal gold (EM.BSA 10, Electron Microscopy Sciences, Hatfield, PA, USA) to facilitate subsequent image alignment ...
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bioRxiv - Cell Biology 2023Quote: ... The fixed sections were washed with distilled water and then incubated with a 0.1% Sirius Red solution (Electron Microscopy Science) for 1 hour at RT ...
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bioRxiv - Cell Biology 2024Quote: Enucleated murine eyes were fixed in 4% paraformaldehyde (PFA; 16% solution stored under argon from Electron Microscopy Sciences Catalog # 15710) diluted in 1X PBS (Fisher Scientific #50-980-487 and Corning 46-013-CM ...
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bioRxiv - Genetics 2023Quote: ... immediately followed by fixation at room temperature for 30 min using a 4% paraformaldehyde solution (Electron Microscopy Sciences, (PN:15714)) in PBS ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Microbiology 2023Quote: ... the ileum was cut away from the rest of the gut and fixed in glutaraldehyde solution (EMS, Hatfield, PA, US) 2.5% in Phosphate Buffer (PB 0.1M pH7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... mice were perfused transcardially with heparinized saline and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Biophysics 2023Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Immunology 2024Quote: ... and cells were fixed and stained with solution containing final concentrations of 2% paraformaldehyde (PFA; Electron Microscopy Sciences, #15713-S) in DPBS ...
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bioRxiv - Microbiology 2021Quote: ... was performed by placing 5 μL drops of the bacteria suspension for 3 minutes directly on glow discharged carbon coated grids (EMS). The grids were then washed with two drops of 2% aqueous uranyl acetate ...
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bioRxiv - Bioengineering 2021Quote: ... the 3D printed constructs were kept in culture for 3 weeks before fixing using paraformaldehyde (4%, Electron Microscopy Sciences, USA) for 45 minutes at RT ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Microbiology 2020Quote: ... 3 µL aliquots of purified ex vivo Enas were deposited on Formvr/Carbon grids (400 Mesh, Cu; Electron Microscopy Sciences), washed with 20 μL 1x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed three times in sterile PBS and fixed in 3% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 μl was applied to a glow discharged (PELCO easiGlow) carbon-coated 300 mesh copper grid (Electron Microscopy Sciences). Grids were stained with 2% uranyl acetate and allowed to dry ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Genomics 2024Quote: Liver tissue sections (3-5 mm thickness) were fixed by submerging in fresh 4% v/v paraformaldehyde (PFA; EMS 15710) in 1X Phosphate Buffered Saline (PBS ...
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Biophysics 2023Quote: U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
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bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) and fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) then fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 ul aliquots of the mixture were applied to UltrAu foil R1.2/1.3 gold 300-mesh grids (Electron Microscopy Sciences). Grids were then blotted and vitrified in liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2024Quote: ... were diluted to[9 mg/ml and aliquots (3[μl) were applied to GF-0.6/1.0 300 Au-flat (Electron Microscopy Sciences). The cryo-EM grids were prepared using Thermo Fisher Vitrobot Mark IV at 8[°C and 100% humidity (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of the diluted DNA solution was dropped onto a carbon film coated TEM grid (Electron Microscopy Sciences, PA, USA), and incubated at room temperature for 1.5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Then the samples were dehydrated in sequential ethanol solutions and embedded in an Eponate mixture (Electron Microscopy Sciences, Hatfield, PA, USA) for polymerization for 24h at 60°C ...