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Citations for Gold Biotechnology :
101 - 142 of 142 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 200 μM auxin (Andole-3-Acetic Acid, Gold Biotechnology) and dTAG7 were added ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were washed in PBS 0.5% BSA (0.5% BSA, Goldbio 9048-46-8) in a round bottom 96-well plate (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology, Cat.# A-420-100) in TBST ...
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bioRxiv - Biophysics 2020Quote: ... Cells were grown in LB media supplemented with 0.2 mg/l of ampicillin (Goldbio) at 37 °C until OD600 of 1.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with D-luciferin (Gold Biotechnology St. Louis, MO; LUCNA-1g) at 37°C and 5% CO2 for one hour in 24 well plate (200 μL/well) ...
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bioRxiv - Cancer Biology 2022Quote: ... All stable cell lines were selected using 2μg/ml of puromycin (Goldbio, P-600). For OVA overexpression ...
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bioRxiv - Microbiology 2021Quote: ... the cell lines were treated with two-fold doubling dilutions of nourseothricin (Gold Biotechnology), S-D ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were induced with 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio). Post induction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 8 ug/ml of blasticidin (Gold Biotechnology, B-800-100) for 48 h to select for cells that had integrated the Xlone-mScarlet-Myo10 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). Sanger sequencing confirmed plasmids that contained the correct protease insert ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). A green-white screen46 was used to inoculate assembled colonies in selection liquid media ...
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bioRxiv - Biophysics 2022Quote: ... C43 (DE3) cells and overexpressed in 1L minimal media A supplemented with ampicillin (Gold Biotechnology) as previously described ...
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bioRxiv - Biochemistry 2022Quote: ... and the cell pellet was resuspended in 0.5mg ml-1 luciferin (GoldBio; cat. no. LUCNA) solution prepared in 1X HBSS buffer (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... Media was aspirated and cells were resuspended in luciferin sodium solution (0.5mg/ml) (Gold Biotech) prepared in 1X Hanks Balanced Salt Solution (HBSS/Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Each CRISPR/Cas9 plasmid construct was independently transformed into DH5-α Chemically Competent Cells (GoldBio) with chloramphenicol (25 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... the total number of target cells was measured by adding D-luciferin potassium salt (Goldbio) to a final concentration of 0.5 mg/ml and reading the bioluminescence with the Infinite F Plex microplate reader (Tecan) ...
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bioRxiv - Microbiology 2023Quote: ... Expression of the provirus was induced by treating cells with doxycycline (GoldBio, St. Louis, MO, USA) at various concentrations ...
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bioRxiv - Biochemistry 2022Quote: ... Stable cells were selected and maintained with 2,500 µg/ml G-418 (Gold Biotechnology, G-418-25) in the medium with the exception of the 293T-based CAPN5-3×FLAG stable cell line that was selected and maintained with 200 µg/ml hygromycin (Gold Biotechnology ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates of Arpin wild-type and mutants C204S and W195D were incubated with glutathioneagarose resin (GoldBio) for 2 h at 4°C (rocking) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with 1× PBS and 75 μL of 2 mM D-luciferin (LUCK-1G, Goldbio) in Leibovitz’s L-15 medium (21083-027 ...
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bioRxiv - Microbiology 2022Quote: ... Transduced cells were selected at 48 hours post-transduction with 8μg/mL Blasticidin S (GoldBio, #B-800-100), expanded ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 0.5-1 cm root tips were harvested with a razor blade and placed into a 35 mm petri dish containing a 70 μm cell strainer and 4.5 mL enzyme solution (1.5% [w/v] cellulase [ONOZUKA R-10, GoldBio] ...
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bioRxiv - Molecular Biology 2019Quote: ... Luminescence assays were set-up in 96-well plates with 1.5 million cells in 200 μl and 500 μM firefly luciferin (GoldBio), 100X dilution of renilla luciferin (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected by centrifugation at 6,000g for 20 min and resuspended in a final volume of 40mL cell lysis buffer (100 mM Tris-HCl, pH 8.0, 1 M NaCl, 20% glycerol, 5 mM TCEP (GoldBio), and 2 cOmplete™ EDTA-free protease inhibitor cocktail tablets (Sigma)) ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were cultured axenically in HL5 medium supplemented with 300 μg/mL streptomycin (GoldBio; #S-150-100) at 22°C ...
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bioRxiv - Biochemistry 2024Quote: ... cells were added to 48 wells of an assay plate and incubated with CTZ400a as the luciferase substrate (GoldBio cat# C-320-1 ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were lysed and assessed for NF-κB activity with an Illumination Firefly Luciferase Enhanced Assay Kit (Gold Biotechnology). The results were normalized by samples’ protein concentrations.
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred μl of cells were mixed with 200 μl of 5 mM D-luciferin K+ salt (GoldBio, LUCK-100) diluted in Tyrode’s buffer and incubated at 28 °C for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Bioluminescence signals from luciferase-expressing cells in live mice were recorded 10 minutes after intraperitoneal injection of D-luciferin (80 mg/kg) (Gold Biotechnology) with IVIS Lumina III Imaging System (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed twice with 1x PBS and lysed using 1x luciferase lysis buffer (L-740, GoldBio, St. Louis, MO USA). Plates were freeze-thawed in -80 freezer ...
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bioRxiv - Immunology 2020Quote: ... Cells were harvested at indicated time points in luciferase lysis buffer and luciferase activity was determined using luciferase reagents (Goldbio,USA) and normalized to β-galactosidase levels (75).
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bioRxiv - Cancer Biology 2021Quote: ... Live-cell luciferase readings were performed for 3 consecutive days by adding 150 μg/mL luciferine (GOLDBIO, Catalog No. LUCK-500) and measuring luminescence in a Tecan Infinite F500 reader (Tecan).
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bioRxiv - Cancer Biology 2022Quote: ... The beads bound with biotinylated cell surface proteins were resuspended in 50 mM Tris (pH 8.5) + 4 M urea + 10 mM TCEP (Gold Biotechnology, TCEP10) and 20 mm IAA (VWR ...
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bioRxiv - Biochemistry 2022Quote: ... FN3-AFF sensors were purified from the soluble fractions of cell lysates using nickel nitriloacetate resin (Gold Biotechnology, St. Louis, MO) following the manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: The biomass obtained from 1 L of cell culture was thawed on ice by adding 200 µL of a protease inhibitor cocktail (500 µM AEBSF (Gold Biotechnology), 1 µM E-64 (Cayman Chemical) ...
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bioRxiv - Systems Biology 2023Quote: ... Permeabilized cells were washed twice with 1X PBS then resuspended in 0.01% Triton-X-100/2% BSA/1X PBS (GoldBio #A-420) at room temperature on a shaker for 1 hour or overnight at 4 °C to block non-specific protein binding ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with 1X PBS and then blocked with 0.5% bovine serum albumin (BSA; Gold Biotechnology, A-420-100) in 1X PBS for 1 hour rocking at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Spectral bioluminescent images of live cells growing in vitro were acquired 2 minutes after adding 150 ug/ml of D-Luciferin (Goldbio, Saint Louis, MO). Sequential images were acquired through optical filters ranging from 520 to 660nm at 20nm intervals ...
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bioRxiv - Biochemistry 2022Quote: ... in the medium with the exception of the 293T-based CAPN5-3×FLAG stable cell line that was selected and maintained with 200 µg/ml hygromycin (Gold Biotechnology, H-270-1).
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bioRxiv - Biochemistry 2022Quote: ... the cleared cell lysate was loaded onto a gravity column with 1 mL bed volume of Glutathione agarose resin (Gold Biotechnology, #G-250-5). The resin was extensively washed ...
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bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
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bioRxiv - Cell Biology 2021Quote: ... tricornutum cell line (maintained in the Allen Lab; McQuaid et al., 2018) was additionally supplemented with 200 μg/mL nourseothricin (GoldBio, Saint Louis, MO, Catalog #N-500-1). All transconjugant P ...