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Citations for Gold Biotechnology :
51 - 100 of 142 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... ten-fold serial dilutions were plated onto synthetic complete agar lacking histidine and containing 0.1% w/v 5-fluoroorotic acid (GoldBio). Plasmid shuffling experiments involving Use1 were conducted in an analogous manner ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in a solubilization buffer containing 2% n-Dodecyl-B-D-Maltoside (DDM, GoldBio, Cat. # DDM10), 350 mM NaCl in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... containing 30 µL of 300 mg/mL timentin (final concentration of 300 mg/L) (T-104-25, Goldbio, USA), followed by homogenization with a T18 digital ULTRA TURRAX homogenizer (IKA ...
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bioRxiv - Neuroscience 2023Quote: ... media was exchanged in each well for 30 μL of Assay Buffer containing 1.2 mM of D-Luciferin (GoldBio). Cells were incubated for at least one hour at 37°C before the addition of 15 μl of compounds diluted in series in Assay Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 3ml assay buffer (HBSS buffer with 0.01% BSA and 5mM HEPES, pH 7.4) containing 10μM coelenterazine (GoldBio; cat. no. CZ05) at final concentration ...
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bioRxiv - Biophysics 2022Quote: ... A transformant colony was used to inoculate Luria–Bertani (LB) media (Fisher Bioreagents) containing 0.1 mg/mL ampicillin (Gold Biotechnology), which was grown overnight (∼15 h ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
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bioRxiv - Biophysics 2019Quote: ... A dense overnight culture from a single transformant was used to inoculate 10 mL of LB broth (LabExpress) containing 0.1 mg/mL ampicillin (Gold Biotechnology) to a starting Abs600nm of 0.0375 ...
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bioRxiv - Cancer Biology 2021Quote: Commensal dysbiosis was initiated in mice by orally gavaging mice daily for 14 days with 100 μl of an antibiotic cocktail containing vancomycin (0.5 mg/ml, Gold Biotechnology), ampicillin (1 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
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bioRxiv - Microbiology 2022Quote: For the lacP1 measurements strains containing the reporter plasmid were grown on LB agar plates with 400 μg/ml Carbenicillin (GoldBio) at 37°C to obtain single colonies ...
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bioRxiv - Biochemistry 2022Quote: ... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (5mM HEPES pH 7.4, 1X HBSS and 0.01% w/v BSA) containing 10µM of coelenterazine (GoldBio, Cat. no: CZ05) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Immunology 2022Quote: ... and antibody was purified using protein A agarose chromatography (Goldbio). Sequences were obtained from PCR reaction products and annotated using the ImMunoGeneTics (IMGT)/V- QUEST database (http://www.imgt.org/IMGT_vquest/)51,52 ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies were isolated from YPD (1% yeast extract, 2% peptone, 2% glucose) agar plates containing 400 μg/ml hygromycin (Gold Biotechnology), and integration was verified by PCR ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 3 ml assay buffer (HBSS buffer with 0.01% BSA and 5 mM HEPES, pH 7.4) containing 10 μM coelenterazine (Goldbio, Cat. no: CZ05) at final concentration ...
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bioRxiv - Physiology 2021Quote: ... in a sealed petri dish containing air-buffered bioluminescence medium (Yamazaki and Takahashi, 2005) plus D-luciferin (100 μM) (Gold Biotechnology) and incubated at 32 °C as previously described (van der Vinne et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and resuspended in assay buffer (1XHBSS, 5 mM HEPES, pH 7.4, 0.01% BSA) containing 10 μM coelenterazine (GoldBio, Cat no. CZ2.5). Resuspended cells were seeded in a white flat bottom 96-well plate (100 μl well-1) ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in the NanoBiT assay buffer (HBSS containing 0.01% BSA, 5 mM HEPES, pH 7.4, and 10 μM coelenterazine (GoldBio, Cat no: CZ05). After resuspension ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Microbiology 2020Quote: ... Strains were patched onto medium containing 50 g/ml X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Gold Biotechnology), 1 mM IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... and screening for loss of b-glucuronidase activity via plating on YT plates containing 50 μg/ml X-Gluc (cyclohexlammonium salt, Gold Biotechnologies, G1281C1). Strains were confirmed by PCR amplification and DNA sequencing of the region of interest.
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bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
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bioRxiv - Physiology 2020Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
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bioRxiv - Molecular Biology 2023Quote: ... Single colonies from the selection plates were grown in selective LB media containing 50 μg/mL ampicillin (Goldbio Cat #: A-301-55) overnight in a 37 °C shaking incubator (180 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 2 % BSA (A-420-500 GoldBio) in TBS-T to the indicated concentration and incubated with membranes overnight at 4C with rocking ...
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bioRxiv - Developmental Biology 2020Quote: ... or fog-2(q71) along with approximately 30 wild type males were placed on RNAi plates [MYOB containing 1mM IPTG (GoldBio, Cat. No. I2481C) and seeded with the appropriate dsRNA expressing HT115 cells] ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was carried out in a 20 µL reaction mixture with extracted RNA and One step RT-qPCR 2x Master Mix containing ROX as a passive reference dye (Gold Biotechnology, St. Louis, MO) and 300 nM forward and reverse primers and 200 nM MGB probe ...
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bioRxiv - Plant Biology 2020Quote: Histochemical GUS staining was done by submerging leaf cuttings in the GUS staining solution containing 1 mM X-Gluc (Gold Biotechnologies, St. Louis, MO, USA) according to Jefferson et al ...
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bioRxiv - Microbiology 2022Quote: ... coli cultures were grown at 37 °C on LC medium containing either kanamycin or spectinomycin (50 or 100 μg/ml, respectively; Gold Biotechnology, Inc., St. Louis, MO) and used as the host for recombinant plasmids made via sequence and ligation-independent cloning (SLIC ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µl diluted cells (2 x104 cells) and 50 µl (8 mg/ml) D-luciferin (GoldBio, USA) were loaded into each well ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were resuspended with 1% low-melt agarose (GoldBio) in PBS at 40°C at ~30 million cells/mL (Fig ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were resuspended with 1% low-melt agarose (GoldBio) in PBS at 40°C at concentrations of ∼15 million cells per milliliter and then loaded into wells of pre-formed phantoms consisting of 1% molecular biology-grade agarose (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were selected in 200µg/mL hygromycin B (Gold Biotechnology), colonies isolated with trypsin-EDTA-soaked filter papers and expanded ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cell lysate was clarified by centrifugation and glutathione agarose resin (GoldBio) was added to affinity purify the GST-GGA3 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were selected using 200 μg/ml hygromycin B (Gold Biotechnology), and single colonies were isolated using trypsin-soaked filter paper ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were induced with various IPTG concentrations (GoldBio, Cat. # 2481C) at 37°C for 3.5 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were selected in 200 μg ml−1 Hygromycin B (Gold Biotechnology) and colonies were isolated and expanded ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then resuspended in 20 μl 1% low-melt agarose (GoldBio) in PBS at 40°C and loaded into the wells of preformed 1% agarose (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... and then stained with the indicated antibody in FACS buffer (PBS with 1% FBS, 10 U/ml DNAse I, GoldBio, St. Louis MO) for 30 minutes on ice with gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Microbiology 2022Quote: ... cells were induced with 50 μM Isopropyl β-d-1-thiogalactopyranoside (IPTG) (GoldBio). Expression was carried out at 8 °C and cells were collected at 20 hours after inoculation by centrifugation at 5000×g for 20 min at 4 °C ...