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Citations for Gold Biotechnology :
101 - 150 of 330 citations for Rat Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates ...
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bioRxiv - Biochemistry 2023Quote: ... ten-fold serial dilutions were plated onto synthetic complete agar lacking histidine and containing 0.1% w/v 5-fluoroorotic acid (GoldBio). Plasmid shuffling experiments involving Use1 were conducted in an analogous manner ...
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bioRxiv - Plant Biology 2023Quote: ... containing 30 µL of 300 mg/mL timentin (final concentration of 300 mg/L) (T-104-25, Goldbio, USA), followed by homogenization with a T18 digital ULTRA TURRAX homogenizer (IKA ...
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bioRxiv - Neuroscience 2023Quote: ... media was exchanged in each well for 30 μL of Assay Buffer containing 1.2 mM of D-Luciferin (GoldBio). Cells were incubated for at least one hour at 37°C before the addition of 15 μl of compounds diluted in series in Assay Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... along with 2.5 μl of a pre-stained protein ladder (#P008; GoldBio, St. Louis, MO), and separated on an SDS-polyacrylamide gel ...
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bioRxiv - Immunology 2021Quote: ... All GST fusion proteins were purified using Glutathione Agarose 4B (Gold Biotechnology, #G-250-50), and eluted with HEPES buffered saline (HBS ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 3ml assay buffer (HBSS buffer with 0.01% BSA and 5mM HEPES, pH 7.4) containing 10μM coelenterazine (GoldBio; cat. no. CZ05) at final concentration ...
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bioRxiv - Biophysics 2022Quote: ... A transformant colony was used to inoculate Luria–Bertani (LB) media (Fisher Bioreagents) containing 0.1 mg/mL ampicillin (Gold Biotechnology), which was grown overnight (∼15 h ...
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bioRxiv - Biophysics 2019Quote: ... A dense overnight culture from a single transformant was used to inoculate 10 mL of LB broth (LabExpress) containing 0.1 mg/mL ampicillin (Gold Biotechnology) to a starting Abs600nm of 0.0375 ...
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bioRxiv - Cancer Biology 2021Quote: Commensal dysbiosis was initiated in mice by orally gavaging mice daily for 14 days with 100 μl of an antibiotic cocktail containing vancomycin (0.5 mg/ml, Gold Biotechnology), ampicillin (1 mg/ml ...
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bioRxiv - Microbiology 2022Quote: For the lacP1 measurements strains containing the reporter plasmid were grown on LB agar plates with 400 μg/ml Carbenicillin (GoldBio) at 37°C to obtain single colonies ...
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bioRxiv - Biochemistry 2022Quote: ... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (5mM HEPES pH 7.4, 1X HBSS and 0.01% w/v BSA) containing 10µM of coelenterazine (GoldBio, Cat. no: CZ05) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... the GltPh proteins were reduced with 500 µM tris(2-carboxy-ethyl) phosphine (TCEP, Gold Biotechnology) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 3 ml assay buffer (HBSS buffer with 0.01% BSA and 5 mM HEPES, pH 7.4) containing 10 μM coelenterazine (Goldbio, Cat. no: CZ05) at final concentration ...
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bioRxiv - Physiology 2021Quote: ... in a sealed petri dish containing air-buffered bioluminescence medium (Yamazaki and Takahashi, 2005) plus D-luciferin (100 μM) (Gold Biotechnology) and incubated at 32 °C as previously described (van der Vinne et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and resuspended in assay buffer (1XHBSS, 5 mM HEPES, pH 7.4, 0.01% BSA) containing 10 μM coelenterazine (GoldBio, Cat no. CZ2.5). Resuspended cells were seeded in a white flat bottom 96-well plate (100 μl well-1) ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in the NanoBiT assay buffer (HBSS containing 0.01% BSA, 5 mM HEPES, pH 7.4, and 10 μM coelenterazine (GoldBio, Cat no: CZ05). After resuspension ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (GoldBio) was added to induce protein expression at 37 °C for 4 h ...
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bioRxiv - Microbiology 2020Quote: ... Strains were patched onto medium containing 50 g/ml X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Gold Biotechnology), 1 mM IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... and screening for loss of b-glucuronidase activity via plating on YT plates containing 50 μg/ml X-Gluc (cyclohexlammonium salt, Gold Biotechnologies, G1281C1). Strains were confirmed by PCR amplification and DNA sequencing of the region of interest.
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bioRxiv - Physiology 2020Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Single colonies from the selection plates were grown in selective LB media containing 50 μg/mL ampicillin (Goldbio Cat #: A-301-55) overnight in a 37 °C shaking incubator (180 rpm) ...
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bioRxiv - Molecular Biology 2023Quote: ... in Krebs-Ringer-HEPES (KRH; pH 7.4) buffer containing 3% fatty acid-free bovine serum albumin (BSA; Gold Biotechnology, St. Louis, NJ), 1 g/L glucose ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The His6-tagged protein variants present in the supernatant were purified using Ni2+-NTA affinity chromatography (Goldbio) and dialyzed overnight at 4 ⁰C against 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology, Olivette, MO), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... and rifampicin (GoldBio #R-120-1, 100 μg mL-1). Bacterial treatments for actin dynamics analysis were conducted following previously described methods36 ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg ml-1 pepstatin A (P-020-100, GoldBio), 100 μg ml−1 4-(2-Aminoethyl ...
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bioRxiv - Developmental Biology 2020Quote: ... or fog-2(q71) along with approximately 30 wild type males were placed on RNAi plates [MYOB containing 1mM IPTG (GoldBio, Cat. No. I2481C) and seeded with the appropriate dsRNA expressing HT115 cells] ...
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bioRxiv - Bioengineering 2022Quote: ... Protein expression was induced by the addition of 1mM isopro-pyl-L-thio-D-galactopyranoside (IPTG) (Gold Biotechnology) at 37°c and after 35 minutes ...
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bioRxiv - Biophysics 2023Quote: ... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Culture growth at 37 °C was monitored by measuring absorbance at 600 nm (A600) and protein expression was induced at A600 = ∼0.7 with 0.5 mM IPTG (GoldBio). The culture was grown at 18 °C for ∼15 hr ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was carried out in a 20 µL reaction mixture with extracted RNA and One step RT-qPCR 2x Master Mix containing ROX as a passive reference dye (Gold Biotechnology, St. Louis, MO) and 300 nM forward and reverse primers and 200 nM MGB probe ...
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bioRxiv - Biochemistry 2024Quote: ... and induced with 1 mM Isopropyl b-d-1-thiogalactopyranoside (IPTG, Goldbio) overnight at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mM IPTG (Goldbio) was added and the cultures were set at 15 °C for 24 h (230 rpm) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM PMSF (GoldBio), 1X Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM luciferin (GOLDBIO) was applied onto leaves and the images were captured using Lumazone imaging system equipped with 2048B CCD camera (Roper).
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bioRxiv - Biochemistry 2024Quote: ... 1 mM TCEP (GoldBio), 5% glycerol (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mM IPTG (GoldBio), 2 mg/ml L-arabinose (GoldBio) ...
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bioRxiv - Microbiology 2022Quote: ... coli cultures were grown at 37 °C on LC medium containing either kanamycin or spectinomycin (50 or 100 μg/ml, respectively; Gold Biotechnology, Inc., St. Louis, MO) and used as the host for recombinant plasmids made via sequence and ligation-independent cloning (SLIC ...
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bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were harvested by centrifugation (6000xg for 20 min) and lysed with B-PER Bacterial Protein Extraction Reagent (Thermo Fischer) supplemented with DNAse I (GoldBio). Lysate was incubated at 60°C for 20 min and cleared by centrifugation at 14,000xg for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) (GoldBio).