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Citations for Gold Biotechnology :
1 - 50 of 330 citations for Rat Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing GST-Rev1 protein was incubated with glutathione agarose resin (Goldbio) for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by adding lysis buffer containing 35 mM reduced glutathione (Gold Biotechnology). The elute fractions were run on a 4-12% NuPAGE Bis-Tris protein gel (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Supernatant containing RBD-Fc was purified over protein A agarose resin (Goldbio, Cat# P-400), cleaved with HRV3C protease (made in house ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/mL leupeptin) containing 1× ProBlock™ Protease Inhibitor Cocktail-50 (GoldBio) and processed as previously described[17] ...
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bioRxiv - Molecular Biology 2023Quote: ... diluting 5X with storage buffer containing 1 mM DTT (GoldBio), and incubating with either 2.5 µl (∼25 units ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 120 µg protein was mixed with substrate containing 15mM ONPG (2-Nitrophenyl-β-D-galactopyranoside, Goldbio, #N27510), 5mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio, Cat. #: GB-116-2). The cortical lysate was then aliquoted into a 96 well plate and stored at −80°C.
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bioRxiv - Cell Biology 2022Quote: ... containing ProBlock Gold™ Mammalian protease inhibitor cocktail (Goldbio, GB-331-1) and Benzonase (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; GoldBio) followed by expression for 17 hr at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM IPTG (GoldBio) and grown at 37°C for 5 h with agitation.
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... 1 µg/mL leupeptin) containing 1x ProBlock™ Protease Inhibitor Cocktail −50 (GoldBio). Total concentration of protein in lysate was quantified using the Pierce™ BCA Protein Assay Kit measured at 562 nm and compared to a standard curve ...
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bioRxiv - Bioengineering 2023Quote: ... AMPK lysis buffer containing 1 mM dithiothreitol (Goldbio Technology, St. Louis, MO, USA), 1mM phenylmethylsulfonyl fluoride (Goldbio Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... in DMEM/F12 containing 1% fatty acid-free bovine serum albumin (Gold Biotechnology) in a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2021Quote: ... Lipin 1 and lipin 1 ΔM-Lip proteins were purified using Ni-NTA resin (GoldBio). Eluted protein was applied to Streptactin-XT resin equilibrated with equilibration buffer (100 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Amersham) and protein expression induced with 1 mM final IPTG (Goldbio) for 4 hr at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... and protein expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (Goldbio, St Lousi, MO, USA). After induction ...
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bioRxiv - Biochemistry 2021Quote: ... and protein expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (Goldbio, St Lousi, MO, USA). After induction ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced for ∼6 h by 1 mM isopropyl β-a-D-1-thiogalactopyranoside (IPTG; GoldBio) in an 18 °C shaker ...
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bioRxiv - Molecular Biology 2021Quote: ... Cultures were cold-shocked on ice for 1 hour and protein expression was induced with 1 mM IPTG (GoldBio). Culture induction was continued for 16 hours 18 °C while shaking ...
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bioRxiv - Biochemistry 2020Quote: ... Induction of protein expression was induced by addition of 1 mM IPTG (Gold Biotech) and cells were grown overnight at 18°C ...
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bioRxiv - Biochemistry 2022Quote: ... followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology) at 18°C for 16 hrs ...
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bioRxiv - Plant Biology 2023Quote: ... The primary transformants were screened on ½ MS plates containing 15 µg ml-1 hygromycin B (Gold Biotechnology). After 2 weeks ...
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bioRxiv - Microbiology 2021Quote: ... penicillin/ streptomycin containing G418 (Goldbio) or G418 plus puromycin (Gibco) ...
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bioRxiv - Biophysics 2019Quote: ... protein expression was then induced with Isopropyl-β-D-1-thiogalactopyranoside 0.1 mM (GoldBio, Canada) for 12 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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bioRxiv - Microbiology 2023Quote: ... containing daptomycin (DAP) (Gold Biotechnology, USA) concentrations of 1X MIC ...
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DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: ... Protein expression is induced with the addition of 50 μL of IPTG (GoldBio 367-93-1) to a 1 mM final concentration ...
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bioRxiv - Microbiology 2020Quote: ... alongside a protein molecular weight marker (BLUEstain protein ladder, Gold Biotechnology). Gels were run at 100 V for 15 min and then 150 V for 40 min in NuPAGE MES SDS running buffer and the proteins were subsequently transferred onto PVDF or nitrocellulose membranes using iBlot transfer stacks (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Cell Biology 2021Quote: ... Glutathione-containing beads (Goldbio G-250-5) were pre-washed with NETN buffer (3x ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies were isolated from YPD (1% yeast extract, 2% peptone, 2% glucose) agar plates containing 400 μg/ml hygromycin (Gold Biotechnology), and integration was verified by PCR ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Immunology 2021Quote: ... RBD proteins were purified using protein A agarose resin (Goldbio CAT# P-400), followed by on-column cleavage with HRV3C protease (made in-house ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced by adding 600 μL of 1 M isopropyl β-D-thiogalactopyranoside (IPTG, Gold Biotechnology) to a final concentration of 1 mM ...
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bioRxiv - Cell Biology 2021Quote: ... containing digitonin (#D-180-250, Gold Biotechnology, USA). After 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... plates containing 200 mg/L hygromycin B (Goldbio). The high expression dsRed-Rho1 vector was created using the splicing by overlap extension method (Higuchi et al. ...
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bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
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bioRxiv - Physiology 2021Quote: ... sections containing SCN (150 μm) were cultured on a Millicell membrane in air-buffered media containing 100 μM D-luciferin (Gold Biotechnology) and imaged for 5 days using a Stanford Photonics XR/MEGA-10Z cooled intensified charge-coupled device camera.
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bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
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bioRxiv - Synthetic Biology 2023Quote: ... containing 100 μg/ml Carbenicillin (Gold Biotechnology, Olivette, MO), 25 μg/ml Chloramphenicol (MilliporeSigma ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...