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Citations for Gold Biotechnology :
51 - 100 of 145 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The bacteria cells were lysed in buffer containing 50 mM HEPES pH 7.4 (#H400, Goldbio), 1.5 M NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Amersham) and protein expression induced with 1 mM final IPTG (Goldbio) for 4 hr at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was filtered and passed over Protein A resin (GoldBio), washed with 10 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified using glutathione agarose resin (Gold Biotechnology Inc.). The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then incubated on ice with serum free media containing 10 nM EGF (Gold Biotechnology) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium was replaced with fresh medium containing 25 μg/ml d-luciferin (GoldBio LUCK-2G). For the luminescence-based readouts of 3D assays ...
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with 100μL of DPBS containing D-luciferin monopotassium salt (40μg/mL, Goldbio) 5 min before imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with 100µL of DPBS containing D-luciferin monopotassium salt (40µg/mL, Goldbio) 5 min before imaging ...
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bioRxiv - Biochemistry 2022Quote: ... The digested protein was passed over a 2-mL Ni-HTC (GoldBio) gravity column to separate the tag-free NnlA ...
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bioRxiv - Immunology 2021Quote: ... The cleaved product was incubated with 1mL of Protein A resin (GoldBio) per 10mg of initial IgG and incubated at room temp for 1 hr to bind any uncleaved IgG and digested Fc ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Bacterial cells were grown in LB broth Miller (Difco) containing chloramphenicol (25 μg/mL) (Gold Biotechnology, USA) at 37 °C to OD600 = 1.7 ...
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bioRxiv - Plant Biology 2023Quote: ... The primary transformants were screened on ½ MS plates containing 15 µg ml-1 hygromycin B (Gold Biotechnology). After 2 weeks ...
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bioRxiv - Microbiology 2023Quote: ... Media containing reduced L-cysteine or reduced glutathione were prepared fresh and supplemented with 2mM TCEP (Goldbio) prior to experimentation ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Immunology 2020Quote: ... The RBD was purified using protein A agarose resin (GoldBio, Cat# P-400) and cleaving the Fc domain using HRV3C protease (made in house ...
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bioRxiv - Biochemistry 2019Quote: ... centrifuged and resuspended in buffer (1XHBSS and 20mM HEPES, pH 7.4) containing 0.5mg/ml luciferin (LUCNA-1G/GOLDBIO). The cells were then seeded in a 96 well plate at a density of 6 × 104 cells /100μl/ well ...
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bioRxiv - Cell Biology 2021Quote: ... Linear fragments were transformed into BY4742 and selected on YPD agar containing nourseothricin sulfate (100 μg/ml, GoldBio).
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bioRxiv - Synthetic Biology 2022Quote: ... The medium was then changed to PBS containing 100 μg/mL of D-luciferin (Gold Biotechnology, LUCNA-500) to obtain luminescence images of firefly luciferase ...
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bioRxiv - Plant Biology 2022Quote: ... ~0.5cm root tips were harvested from 1000-3000 roots and placed into a 35mm petri dish containing a 70 μm cell strainer and 4.5mL enzyme solution (1.5% [w/v] cellulase [ONOZUKA R-10, GoldBio] ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were injected intraperitoneally with 100 μl of PBS containing D-luciferin monopotassium salt (40 mg/ml) (Goldbio) and mice were anaesthetized with isoflurane 2 min before imaging ...
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bioRxiv - Microbiology 2021Quote: ... 200 uL of the suspension was plated on selective LB agar plates containing nourseothricin (GoldBio, St Louis, MO) at multiple two-fold doubling dilution concentrations and incubated for 48-72 hours at 35°C in ambient air until mutant colonies became visible ...
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bioRxiv - Molecular Biology 2023Quote: ... spread on selective Luria broth (LB) agar plates containing 50 μg/mL carbenicillin (GoldBio Cat #: C-103-25), and grown in a 37 °C incubator overnight ...
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bioRxiv - Microbiology 2024Quote: ... The cells were grown in Luria Broth Media (Research Product Internationals) containing 50 µg/mL Kanamycin (Gold Biotechnology) at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... expression of recombinant protein was induced with 0.5 mM isopropyl β-D-thiogalactoside (GoldBio). For purification of PABPC1 and ePAB ...
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bioRxiv - Biochemistry 2020Quote: ... Induction of protein expression was induced by addition of 1 mM IPTG (Gold Biotech) and cells were grown overnight at 18°C ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; GoldBio) followed by expression for 17 hr at 25°C ...
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bioRxiv - Biochemistry 2022Quote: ... followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology) at 18°C for 16 hrs ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in a solubilization buffer containing 2% n-Dodecyl-B-D-Maltoside (DDM, GoldBio, Cat. # DDM10), 350 mM NaCl in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates ...
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bioRxiv - Biochemistry 2023Quote: ... ten-fold serial dilutions were plated onto synthetic complete agar lacking histidine and containing 0.1% w/v 5-fluoroorotic acid (GoldBio). Plasmid shuffling experiments involving Use1 were conducted in an analogous manner ...
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bioRxiv - Plant Biology 2023Quote: ... containing 30 µL of 300 mg/mL timentin (final concentration of 300 mg/L) (T-104-25, Goldbio, USA), followed by homogenization with a T18 digital ULTRA TURRAX homogenizer (IKA ...
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bioRxiv - Neuroscience 2023Quote: ... media was exchanged in each well for 30 μL of Assay Buffer containing 1.2 mM of D-Luciferin (GoldBio). Cells were incubated for at least one hour at 37°C before the addition of 15 μl of compounds diluted in series in Assay Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant containing RPA4AZP was applied to a 3 mL pre-equilibrated Ni2+-NTA (Gold Biotechnology Inc., USA) gravity column ...
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bioRxiv - Biophysics 2019Quote: ... protein expression was then induced with Isopropyl-β-D-1-thiogalactopyranoside 0.1 mM (GoldBio, Canada) for 12 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... along with 2.5 μl of a pre-stained protein ladder (#P008; GoldBio, St. Louis, MO), and separated on an SDS-polyacrylamide gel ...
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bioRxiv - Biochemistry 2021Quote: ... Lipin 1 and lipin 1 ΔM-Lip proteins were purified using Ni-NTA resin (GoldBio). Eluted protein was applied to Streptactin-XT resin equilibrated with equilibration buffer (100 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... All GST fusion proteins were purified using Glutathione Agarose 4B (Gold Biotechnology, #G-250-50), and eluted with HEPES buffered saline (HBS ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 3ml assay buffer (HBSS buffer with 0.01% BSA and 5mM HEPES, pH 7.4) containing 10μM coelenterazine (GoldBio; cat. no. CZ05) at final concentration ...
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bioRxiv - Biophysics 2022Quote: ... A transformant colony was used to inoculate Luria–Bertani (LB) media (Fisher Bioreagents) containing 0.1 mg/mL ampicillin (Gold Biotechnology), which was grown overnight (∼15 h ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and resuspended in assay buffer containing D-luciferin (0.5 mg ml−1, GoldBio, Cat. no. LUCNA-1G) in 1X HBSS ...
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bioRxiv - Biophysics 2019Quote: ... A dense overnight culture from a single transformant was used to inoculate 10 mL of LB broth (LabExpress) containing 0.1 mg/mL ampicillin (Gold Biotechnology) to a starting Abs600nm of 0.0375 ...
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bioRxiv - Cancer Biology 2021Quote: Commensal dysbiosis was initiated in mice by orally gavaging mice daily for 14 days with 100 μl of an antibiotic cocktail containing vancomycin (0.5 mg/ml, Gold Biotechnology), ampicillin (1 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
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bioRxiv - Microbiology 2022Quote: For the lacP1 measurements strains containing the reporter plasmid were grown on LB agar plates with 400 μg/ml Carbenicillin (GoldBio) at 37°C to obtain single colonies ...
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bioRxiv - Biochemistry 2022Quote: ... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (5mM HEPES pH 7.4, 1X HBSS and 0.01% w/v BSA) containing 10µM of coelenterazine (GoldBio, Cat. no: CZ05) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...