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Citations for Gold Biotechnology :
1 - 50 of 145 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing GST-Rev1 protein was incubated with glutathione agarose resin (Goldbio) for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by adding lysis buffer containing 35 mM reduced glutathione (Gold Biotechnology). The elute fractions were run on a 4-12% NuPAGE Bis-Tris protein gel (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... and antibody was purified using protein A agarose chromatography (Goldbio). Sequences were obtained from PCR reaction products and annotated using the ImMunoGeneTics (IMGT)/V- QUEST database (http://www.imgt.org/IMGT_vquest/)51,52 ...
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bioRxiv - Immunology 2020Quote: Supernatant containing RBD-Fc was purified over protein A agarose resin (Goldbio, Cat# P-400), cleaved with HRV3C protease (made in house ...
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bioRxiv - Molecular Biology 2022Quote: ... 120 µg protein was mixed with substrate containing 15mM ONPG (2-Nitrophenyl-β-D-galactopyranoside, Goldbio, #N27510), 5mM DTT ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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bioRxiv - Microbiology 2021Quote: ... penicillin/ streptomycin containing G418 (Goldbio) or G418 plus puromycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... containing daptomycin (DAP) (Gold Biotechnology, USA) concentrations of 1X MIC ...
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bioRxiv - Microbiology 2020Quote: ... alongside a protein molecular weight marker (BLUEstain protein ladder, Gold Biotechnology). Gels were run at 100 V for 15 min and then 150 V for 40 min in NuPAGE MES SDS running buffer and the proteins were subsequently transferred onto PVDF or nitrocellulose membranes using iBlot transfer stacks (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Glutathione-containing beads (Goldbio G-250-5) were pre-washed with NETN buffer (3x ...
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bioRxiv - Immunology 2021Quote: ... RBD proteins were purified using protein A agarose resin (Goldbio CAT# P-400), followed by on-column cleavage with HRV3C protease (made in-house ...
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bioRxiv - Cell Biology 2021Quote: ... containing digitonin (#D-180-250, Gold Biotechnology, USA). After 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... plates containing 200 mg/L hygromycin B (Goldbio). The high expression dsRed-Rho1 vector was created using the splicing by overlap extension method (Higuchi et al. ...
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bioRxiv - Microbiology 2020Quote: ... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
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bioRxiv - Physiology 2021Quote: ... sections containing SCN (150 μm) were cultured on a Millicell membrane in air-buffered media containing 100 μM D-luciferin (Gold Biotechnology) and imaged for 5 days using a Stanford Photonics XR/MEGA-10Z cooled intensified charge-coupled device camera.
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bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
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bioRxiv - Synthetic Biology 2023Quote: ... containing 100 μg/ml Carbenicillin (Gold Biotechnology, Olivette, MO), 25 μg/ml Chloramphenicol (MilliporeSigma ...
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bioRxiv - Biophysics 2020Quote: ... containing 50 μg/mL carbenicillin (Goldbio, St Lousi, MO, USA), and protein expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (Goldbio ...
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bioRxiv - Biochemistry 2021Quote: ... containing 50 μg/mL carbenicillin (Goldbio, St Lousi, MO, USA), and protein expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (Goldbio ...
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bioRxiv - Molecular Biology 2023Quote: ... diluting 5X with storage buffer containing 1 mM DTT (GoldBio), and incubating with either 2.5 µl (∼25 units ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in media containing 0.1mM D-luciferin (Goldbio) and placed under photomultiplier tubes (PMTs ...
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced with IPTG (GoldBio, I2481C) at 16°C for 18 hours ...
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bioRxiv - Neuroscience 2021Quote: ... containing phosphatase and protease inhibitor cocktail (Goldbio Cat. No. GB-108-2). Western blotting was performed using 40 μg protein/well as previously described (Mohammed et al ...
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bioRxiv - Cell Biology 2022Quote: ... containing ProBlock Gold™ Mammalian protease inhibitor cocktail (Goldbio, GB-331-1) and Benzonase (Sigma ...
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bioRxiv - Immunology 2021Quote: ... followed by purification with protein A agarose resin (GoldBio). Expi293F cells were cultured in Expi293 Expression Medium (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Protein expression was induced by adding 0.2 % arabinose (Goldbio) for 3 hr at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... and mAbs were purified with protein A agarose (GoldBio).
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bioRxiv - Immunology 2020Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio). The resin was collected in a chromatography column ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with Protein A Agarose Resin (GoldBio) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... strains were selected on yeast peptone 2% dextrose (YPD) plates containing nourseothricin (GoldBio), hygromycin B (Thermo Fisher Scientific) ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... 1 µg/mL leupeptin) containing 1x ProBlock™ Protease Inhibitor Cocktail −50 (GoldBio). Total concentration of protein in lysate was quantified using the Pierce™ BCA Protein Assay Kit measured at 562 nm and compared to a standard curve ...
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bioRxiv - Bioengineering 2023Quote: ... AMPK lysis buffer containing 1 mM dithiothreitol (Goldbio Technology, St. Louis, MO, USA), 1mM phenylmethylsulfonyl fluoride (Goldbio Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/mL leupeptin) containing 1× ProBlock™ Protease Inhibitor Cocktail-50 (GoldBio) and processed as previously described[17] ...
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bioRxiv - Biochemistry 2023Quote: ... and 35 ml starter culture with 2xTY containing 100 µg/ml carbenicillin (Goldbio) was grown for 16 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... in DMEM/F12 containing 1% fatty acid-free bovine serum albumin (Gold Biotechnology) in a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2020Quote: ... recombinants were selected on YPD agar plates containing 100 μg/ml nourseothricin (Gold Biotechnology), resulting in ySI42 ...
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bioRxiv - Cell Biology 2021Quote: ... the media was replaced with fresh media containing puromycin (Gold Biotechnology; P-600-100) for selection ...
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bioRxiv - Neuroscience 2023Quote: ... containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio, Cat. #: GB-116-2). The cortical lysate was then aliquoted into a 96 well plate and stored at −80°C.
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bioRxiv - Cell Biology 2020Quote: ... or Protein A Agarose Resin (Gold Biotechnology, P-400-5). Beads and antibody were incubated and rotated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Bulk IgGs were captured by protein A agarose resin (GoldBio) at 4°C rotating overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Heterologous protein expression was induced with 0.5 mM IPTG (GoldBio), and cultures were grown overnight at 19°C with gentle agitation.
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 0.5 mM IPTG (GoldBio) and grown overnight at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM IPTG (GoldBio) and grown at 37°C for 5 h with agitation.
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 0.5 mM IPTG (GoldBio) followed by growth overnight at 16°C ...
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bioRxiv - Immunology 2023Quote: WP and HIP were mixed with Protein A-agarose (GoldBio), Protein G-agarose (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... NGM dishes containing 50 µg/mL ampicillin (A-301-25, Gold Biotechnology, St. Louis, MO) and 1 mM IPTG (isopropyl-β-D-thiogalactoside ...