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Citations for Gold Biotechnology :
351 - 400 of 406 citations for D Ribitol 5 Phosphate Cytidylyltransferase ISPD CRPPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Mice were imaged on the IVIS every 3-4 days after being sedated with Isoflurane and intraperitoneal injections of ∼120 mg/kg D-luciferin (GoldBio, St. Louis, MI). Mice were injected with 100,000 IU of human IL-2 (National Cancer Institute ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor progression was evaluated by luminescence emission on a Xenogen IVIS spectrum after intraperitoneal injection of D-luciferin (Gold Biotechnology, St. Louis, MO) injection according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2022Quote: ... Antibiotics used in this study include kanamycin (GoldBio #K-120-5, 50 μg mL-1), and rifampicin (GoldBio #R-120-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were applied to GST agarose column (Glutathione agarose resin, GoldBio Cat# G-250-5) and washed 3 times in wash buffer 1x TBS + 1mM DTT) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were co-cultured overnight and luminescence was measured the next morning after addition of 150 μg/ml D-luciferin (Gold Biotechnology Cat# LUCNA-2G) and subsequent incubation for 5 mins at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The virus-serum mix was subsequently added to Vero cells for 48 h before 50 nmoles of D-Luciferin (GoldBio, St Lous, MO, USA) was added to measure luminescence ...
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bioRxiv - Bioengineering 2024Quote: ... The culture was then quickly cooled on ice for 10-15 minutes and induced with 1 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology, Cat# I2481C100). For CODE constructs that were built based on the pET-PE2-His backbone (Addgene ...
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bioRxiv - Neuroscience 2024Quote: The following chemicals and reagents were purchased from the manufacturers described: isopropyl β-D-1-thiogalactopyranoside (IPTG) from Gold Biotechnology (St. Louis, MO, USA), L-arabinose ...
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bioRxiv - Biophysics 2022Quote: ... Tris-(carboxyethyl) phosphine hydrochloride (TCEP) and adenosine-5’-triphosphate (ATP) disodium trihydrate were purchased from GoldBio. Imidazole was purchased from Acros Organics ...
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bioRxiv - Cancer Biology 2021Quote: ... the medium was supplemented with 100 μg/ml hygromycin (#H-270-5; Goldbio, St Louis MO). For serum stimulation ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% (w/v) bovine serum albumin (BSA; Gold Biotechnology, A-420-100) in tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble fraction was then applied to a 5 mL packed Ni-NTA resin bed (GoldBio) and equilibrated with 10 column volumes of TBS ...
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bioRxiv - Biochemistry 2024Quote: ... The clarified lysate was first batch-bound to 5 ml Ni-NTA resin (Gold Biotechnology Inc.) for 3 hours ...
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bioRxiv - Immunology 2022Quote: ... and antibody was purified using protein A agarose chromatography (Goldbio). Sequences were obtained from PCR reaction products and annotated using the ImMunoGeneTics (IMGT)/V- QUEST database (http://www.imgt.org/IMGT_vquest/)51,52 ...
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bioRxiv - Physiology 2022Quote: ... N2 worms were egg-prepped onto solid NGM with 50 μg/ml carbenicillin (GoldBio, C-103-5) and HT115(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% bovine serum albumin (BSA, #A-420-1, Gold Biotechnology, St. Louis, MO), for phospho-specific antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... seeds were grown in MS/2 medium containing 75 µM 5-azacytidine (Goldbio, St. Louis, MO, USA), 0.1 or 0.5 µM picloram (Duchefa Biochemie ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then blocked in 5% bovine serum albumin (#A-420-10, GoldBio, St. Louis, MO, USA), or milk ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 2 % BSA (A-420-500 GoldBio) in TBS-T to the indicated concentration and incubated with membranes overnight at 4C with rocking ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were shaved front and back and Nair was applied to remove remainder of the hair before being IP injected with D-luciferin (Goldbio LUCK-1G, 150mg/kg in 150 mL). Mice are placed in a supine position on the light-tight chamber of the CCD camera imaging unit ...
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bioRxiv - Cell Biology 2022Quote: ... the procedure was similar except the culture media also contained 5ng/ml GM-CSF (GoldBio # 1320-03-5) to stimulate microglial proliferation ...
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bioRxiv - Developmental Biology 2024Quote: ... plants were incubated with an aqueous solution of 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A) for 30 minutes.
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bioRxiv - Biochemistry 2024Quote: ... Transformants were grown overnight at 30 °C in 5 mL 1.5xYT media with 25 µg/mL chloramphenicol (GoldBio) and sub-cultured 1:100 and grown at 37 °C until mid-log phase (∼2 hours) ...
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bioRxiv - Biophysics 2021Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 5 μg/mL freshly prepared coelenterazine 400a (GoldBio). Cells were then placed in white-walled ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected by centrifugation at 6,000g for 20 min and resuspended in a final volume of 40mL cell lysis buffer (100 mM Tris-HCl, pH 8.0, 1 M NaCl, 20% glycerol, 5 mM TCEP (GoldBio), and 2 cOmplete™ EDTA-free protease inhibitor cocktail tablets (Sigma)) ...
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bioRxiv - Molecular Biology 2023Quote: ... with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates ...
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bioRxiv - Biophysics 2024Quote: ... Cleared lysate was loaded onto lysis buffer-equilibrated Ni-NTA resin (GoldBio, 5 mL resin per 50 mL lysate) and rocked gently for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... then the cleared bacterial lysate was passed through a 0.22 μm PES filter (Genessee Scientific) and then applied to a 5 mL packed Ni-NTA agarose beads (Goldbio) equilibrated with Lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... ten-fold serial dilutions were plated onto synthetic complete agar lacking histidine and containing 0.1% w/v 5-fluoroorotic acid (GoldBio). Plasmid shuffling experiments involving Use1 were conducted in an analogous manner ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were transferred onto fresh media and treated with 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A).
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bioRxiv - Cell Biology 2024Quote: ... 100 µg of each sample was incubated for 30 min at 37°C with 5 mM TCEP (Gold Biotechnology) for disulfide bond reduction with subsequent alkylation with 20 mM Iodoacetamide (Sigma I6125 ...
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Microbiology 2020Quote: ... The protein eluted and collected in 5-1mL aliquots of elution buffer (50 mM NaH2PO4, 300 mM NaCl, and 250 mM imidazole (GoldBio) (pH 8.0)) ...
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bioRxiv - Cell Biology 2022Quote: ... animals were grown on 6 cm NGM plates that were supplemented with 250 μl of 5 mg/ml of hygromycin B (GoldBio) in M9.
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bioRxiv - Cell Biology 2023Quote: ... The transformants were selected for 4-5 days in 1 ml of YPD broth with clonNAT (200 µg/mL, GoldBio), resulting in the intermediate MTS-mCherry-GFP1-10-clonNat library ...
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bioRxiv - Biochemistry 2022Quote: ... #DF0446-07-5) or 7H10 (Difco, cat# DF0627-17-4) plates containing 50 ug/mL hygromycin (GoldBio, cat. #H-270) and incubated at 37° C for 3-5 days ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Resident eIF1A p3392 was replaced through plasmid shuffling by plating on media containing 5-fluoroorotic acid (GoldBio, cat# F230-25), as described previously (16 ...
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bioRxiv - Microbiology 2024Quote: ... into electrocompetent BTH101 and plated on LB containing 40 μg/mL X-gal (5-bromo-4-chloro-3-ondolyl-beta-galacto-pyranoside) (GoldBio), 0.5 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µL of media from each well was added to 10 µL of non-lytic 2x coelenterazine reagent (300 mM sodium ascorbate, 5 mM NaCl, 0.1% BSA, 40 μM coelenterazine (Goldbio CZ25)) in separate wells of a 384-well plate ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 3 ml assay buffer (HBSS buffer with 0.01% BSA and 5 mM HEPES, pH 7.4) containing 10 μM coelenterazine (Goldbio, Cat. no: CZ05) at final concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... each plate was sprayed with 1 milliliter of an aqueous solution of 5 mM firefly luciferin (Gold Biotechnology, St Louis, MO) and 0.01% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Biochemistry 2022Quote: ... and resuspended in assay buffer (1XHBSS, 5 mM HEPES, pH 7.4, 0.01% BSA) containing 10 μM coelenterazine (GoldBio, Cat no. CZ2.5). Resuspended cells were seeded in a white flat bottom 96-well plate (100 μl well-1) ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in the NanoBiT assay buffer (HBSS containing 0.01% BSA, 5 mM HEPES, pH 7.4, and 10 μM coelenterazine (GoldBio, Cat no: CZ05). After resuspension ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... The G418-resistance cassettes were transformed into FY4 (MATa) and selected on YPD + G418 (400 µg/mL; Gold Biotechnology #G-418-5) plates ...
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bioRxiv - Biochemistry 2024Quote: ... Media supernatant was further filtered using 0.2 μm PVDF vacuum filter prior to incubation with Ni-NTA agarose resin (GoldBio, H-350-5) (10 mL wet volume per 1 L supernatant ...
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...