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Citations for Gold Biotechnology :
301 - 350 of 406 citations for D Ribitol 5 Phosphate Cytidylyltransferase ISPD CRPPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells were plated at equal density and luminescence signals were quantified in the presence of 150 µg/ml D-luciferin (GoldBio) by imaging using cooled CCD optical macroscopic imaging system (IVIS Lumina Series III ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 minutes after subcutaneous injection of 100 μl (150 mg Luciferin/kg body weight) of D-luciferin (Goldbio LUCK-1G). After the final week ...
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bioRxiv - Biochemistry 2024Quote: ... Whole leaves were removed after 4 days of incubation and were sprayed with a solution containing 1 mM D-luciferin (GoldBio) and 0.01% Silwett L-77 (Lehle Seeds Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... the mice were injected intraperitoneally with 10 μl/g of 15 mg/mL D-luciferin potassium salt solution (Gold Biotechnology) and imaged using the Xenogen IVIS 200 In Vivo Imaging System (PerkinElmer) ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Cell Biology 2023Quote: ... and added to the cell medium to 1 μM final concentration. D-luciferin sodium salt (Cat. #LUCNA) and coelenterazine (Cat. #CZ) were purchased from GoldBio and resuspended to 100 mM in 10 mM HEPES buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were anesthetized and injected retro-orbitally with 1.5 mg (75 mg/kg) of D-luciferin (Gold Biotechnology; LUCK-1G) at the indicated times after injecting cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Orthotopic and tail-vein implanted mouse lung metastases were assessed by serial IVIS imaging after administration of 2mg/mouse D-Luciferin (GOLDBIO). Bioluminescence images were captured using the IVIS spectrum CT In Vivo imaging system (Caliper Life Sciences) ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were incubated at 37 °C until the absorbance at 600 nm reached 0.7-0.9 at which point 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) was added ...
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bioRxiv - Biophysics 2024Quote: ... The membrane was solubilized for 2 h at 4°C on a roller shaker at 30 rpm in 1% n-dodecyl-B-D-maltoside (DDM) (GoldBio), 0.2% CHS (Anatrace ...
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bioRxiv - Biochemistry 2020Quote: ProBlock Gold Mammalian Protease Inhibitor Cocktail (Gold Biotechnology, Cat. # GB-331-5)
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bioRxiv - Biochemistry 2021Quote: ProBlock Gold Mammalian Protease Inhibitor Cocktail (Gold Biotechnology, Cat. # GB-331-5)
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bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 and 2 mM Tris (2-carboxyethyl) phosphine (TCEP, GoldBio).
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bioRxiv - Biochemistry 2023Quote: ... coli and isolated using nickel agarose beads (Goldbio, Cat# H-350-5). 5 μM of HaBlap was mixed with 50 μM nitrocefin in 5 mM phosphate buffer pH 7 ...
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bioRxiv - Synthetic Biology 2023Quote: ... purified using Nickel NTA agarose beads (Catalog No. H-350-5, GoldBio), and eluted (500 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... mice were anesthetized with 2.5% isoflurane (Fujifilm Wako) and intraperitoneally injected with 3 mg D-luciferin (Gold Biotechnology Inc., MO, USA) in 200 μl PBS as previously described [52 ...
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bioRxiv - Cancer Biology 2022Quote: ... Bioluminescence signals from luciferase-expressing cells in live mice were recorded 10 minutes after intraperitoneal injection of D-luciferin (80 mg/kg) (Gold Biotechnology) with IVIS Lumina III Imaging System (PerkinElmer) ...
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bioRxiv - Biophysics 2020Quote: ... 1% of primary culture was inoculated in 750 mL of LB media and induced with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology) at OD600 ~ 0.6 and further incubated at 16 °C for 14–16 h with constant shaking at 200 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
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bioRxiv - Physiology 2021Quote: ... in a sealed petri dish containing air-buffered bioluminescence medium (Yamazaki and Takahashi, 2005) plus D-luciferin (100 μM) (Gold Biotechnology) and incubated at 32 °C as previously described (van der Vinne et al. ...
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bioRxiv - Physiology 2021Quote: ... sections containing SCN (150 μm) were cultured on a Millicell membrane in air-buffered media containing 100 μM D-luciferin (Gold Biotechnology) and imaged for 5 days using a Stanford Photonics XR/MEGA-10Z cooled intensified charge-coupled device camera.
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bioRxiv - Cancer Biology 2023Quote: ... Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G, Goldbio, USA) or 1 volume of 0.5 mg/mL Nano-Glo In Vivo Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were grown at 37 °C to an OD600 of 0.8 and then induced using 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG; GoldBio, 2481C) for 8 h at 30 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor volume was monitored through bioluminescence using IVIS Spectrum apparatus (Caliper Life Sciences) after i.p injection of D-luciferin 150mg/Kg (GOLDBIO #LUCK-1G). Mice were sacrificed when manifested more than 20% of body weight loss or neurological symptoms ...
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bioRxiv - Neuroscience 2023Quote: ... Bacteria were grown to an OD600 of 0.6 and induced with 500 µM isopropyl β-D-1-thioga-lactopyranoside (IPTG) (GoldBio, I2481C) for 18 h at 18°C ...
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bioRxiv - Biochemistry 2024Quote: ... media in the wells was replaced with the assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G). Luminescence was recorded immediately in a microplate reader (BMG Labtech) ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in assay buffer (20mM HEPES pH 7.4, 1X Hank’s Balanced Salt Solution/ HBSS and 0.5mg/mL D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well plates at a density of 100,000 cells/well ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase expression was checked by using in vitro bioluminescence imaging by adding 100 μL of 15 mg mL-1 D-luciferin solution (#LUCNA-1G, Goldbio, USA) to the cells and imaged after 2 min using a Fusion FX system (Vilber ...
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bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
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bioRxiv - Cell Biology 2023Quote: ... into a centrifuge tube and then lysed with lysis buffer (50mM Tris, pH 7.5, 150mM NaCl, and 2 mM Dodecyl-B-D-maltoside (DDM) (GoldBio, catalog #: DDM5) supplemented with complete protease inhibitor cocktail (Roche #04693116001) ...
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bioRxiv - Microbiology 2024Quote: ... 25 µL of the overnight culture was inoculated in 4 mL triplicates of minimal media (M9) supplemented with 100µM Isopropyl β-d-1-thiogalactopyranoside (IPTG, GoldBio, I2481C25), 100 µg/mL carbenicillin ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were injected intraperitoneally with 100 μL of a 30 mg/ml D-Luciferin potassium salt solution (LUCK-100, Gold Biotechnology) 10 min before imaging and were then anesthetized using 2% isoflurane ...
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bioRxiv - Physiology 2023Quote: ... 5 mL of MS-EGTA buffer with 0.2% BSA (Goldbio #A-421-250) is added to the tube to stop the trypsin reaction ...
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bioRxiv - Biochemistry 2022Quote: ... cells were detached from the plates, resuspended in assay buffer (1XHBSS, 20 mM HEPES, pH7.4) containing D-luciferin (0.5 mg ml-1, GoldBio, Cat. no. LUCNA-1G) and seeded into 96 well white plates (Corning ...
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bioRxiv - Microbiology 2020Quote: ... ATP-free or ATP-containing (150 μM ATP) substrate solutions were prepared containing 150 μg/mL D-Luciferin (Goldbio, St. Louis, MO), 100 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... the culture was expanded 1:100 up to 2L cultures in Luria broth and induced at A600 ∼0.60 with 0.5 mM isopropyl β-D-thiogalactopyranoside (GoldBio, St. Louis MO) for 24 h at 16°C ...
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bioRxiv - Cell Biology 2021Quote: NSG mice transplanted with GAPDHLuciferase/wt and INSGFP/wt double reporter hESCs lines were i.p injected with 150mg/kg body weight of D-luciferin potassium salt (Gold Biotechnology, luck-2G) in PBS 15 minutes before imaging on a IVIS spectrum optical imaging system (PerkinElmer) ...
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bioRxiv - Biochemistry 2023Quote: ... 20 mM of 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.4 and D-luciferin (0.5 mg ml-1) (GoldBio, Cat. no.: LUCNA-1G). Harvested cells were then seeded in an opaque flat bottom white 96 well cell culture plate (SPL life sciences ...
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bioRxiv - Cell Biology 2023Quote: ... were spotted onto LB plates supplemented with 100 µg/mL ampicillin with or without 1 mM isopropyl β-D-1-thiogalacopyranoside (IPTG, Gold Biotechnology). After drying ...
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bioRxiv - Biophysics 2023Quote: ... resuspended in the assay buffer (20mM HEPES pH 7.4 and 1X HBSS) containing 0.5mg ml-1 of D-luciferin (GoldBio, Cat. no: LUCNA-1G) and seeded in 96-well white plates (SPL Life Sciences ...
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bioRxiv - Bioengineering 2023Quote: ... Images were acquired 10 min after intraperitoneal injection of 3 mg d-Luciferin Potassium Salt (Gold Biotechnology, Inc., St Louis MO, USA) in 200 μL 0.1 M phosphate buffered saline ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and lysed with lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM n-Dodecyl-β-D-maltoside (DDM) (GoldBio, catalog #: DDM5)) supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2024Quote: Tumor growth was monitored by bioluminescence on a Xenogen IVIS Spectrum 10 min after intraperitoneal injection of 150 mg/kg D-luciferin prepared according to the manufacturer’s directions (Gold Biotechnology, #LUCK-100). The total flux signal was used for quantification in all studies.
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced when the culture reached an OD600 = 2 by addition of isopropyl β-D-1-thiogalactopyranoside (IPTG) (GoldBio I2481) at a final concentration of 400 μM ...
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bioRxiv - Cancer Biology 2024Quote: Mice were injected with 100 uL of 15 mg/mL D-luciferin potassium salt in DPBS (no Ca, no Mg) (GoldBio, #LUCK-100), then anesthetized with isoflurane ...
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bioRxiv - Microbiology 2021Quote: ... Ampicillin sodium (cat #A-301-5) was purchased from Gold Biotechnology (St. Louis, MO).
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bioRxiv - Cell Biology 2021Quote: ... Successful integration was positively selected for using G418 (GoldBio Cat no. G-418-5); animals retaining an extrachromosomal array were killed by heat-shock (2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... then stimulated with the coelenterazine 400A substrate (5 mM) (Gold Biotechnology #C-320-1) and read for 30 min on Mithras2 Plate Reader (Berthold ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were lysed and reduced with 5 mM DTT (Gold Biotechnology, St-Louis, MO) at 37 °C for 1 h and alkylated with 15 mM IAA (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Spectral bioluminescent images of live cells growing in vitro were acquired 2 minutes after adding 150 ug/ml of D-Luciferin (Goldbio, Saint Louis, MO). Sequential images were acquired through optical filters ranging from 520 to 660nm at 20nm intervals ...