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Citations for Gold Biotechnology :
151 - 200 of 294 citations for Mouse Anti Toxoplasma gondii SAG 1 protein 6207 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 1 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Gold Biotechnology) was used for induction of the Pspac promoter or its derivatives ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were resuspended with 1% low-melt agarose (GoldBio) in PBS at 40°C at ~30 million cells/mL (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... and IPTG (Isopropyl-β-D-1-thiogalactopyranoside) (Gold Biotechnology) was added to the bacterial solution to final concentration of 1mM to induce the protein expression under the control of T7 promoter ...
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bioRxiv - Microbiology 2024Quote: ... 0.1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) (GoldBio), and 40 μg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM Tris (2-carboxyethyl) phosphine (TCEP; GoldBio, TCEP25), and 25 mM imidazole (Fischer Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were resuspended with 1% low-melt agarose (GoldBio) in PBS at 40°C at concentrations of ∼15 million cells per milliliter and then loaded into wells of pre-formed phantoms consisting of 1% molecular biology-grade agarose (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... 3 μg ml−1 aprotinin (A-655-100, GoldBio), 1 mM benzamidine (B-050-100 ...
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bioRxiv - Microbiology 2023Quote: ... or 20 μg ml-1 kanamycin (Goldbio, K-120) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 % Pen/Strep and 5 µg/ml Puromycin (Goldbio). Cells were seeded into collagen-coated 24-well plates (50 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM dithiothreitol (cat # DTT10, GoldBio; St Louis, MO), 500 µM ascorbic acid ...
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bioRxiv - Plant Biology 2024Quote: ... was added to each reaction and then loaded onto a 1% agarose/TBE gel along with 5 µl each of 100 bp Plus and 1 kb Plus DNA ladders (Goldbio, Saint Louis, MO). The gel was run at 105V for 45 min and stained with 1X RedSafe (Bulldog Bio ...
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bioRxiv - Bioengineering 2023Quote: ... The cultures were grown at 37°C at 220 rpm and induced at an OD600 of 1-1.2 using 1 mM isopropylthio-β-galactoside (IPTG) (GoldBio, St. Lois, MO, USA). Cultures for fluorescence imaging were grown C aerobically at 16° for 16h post-induction ...
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bioRxiv - Bioengineering 2024Quote: ... The culture was then quickly cooled on ice for 10-15 minutes and induced with 1 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG) (Gold Biotechnology, Cat# I2481C100). For CODE constructs that were built based on the pET-PE2-His backbone (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... After 15 min incubation with 1 mg/ml lysozyme (Goldbio) and 20 ug/ml DNase (Millipore-Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... eIF2α expression was induced with 1 mM IPTG (Gold Biotechnology) and the culture was grown for 16 hours at 16 °C ...
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bioRxiv - Biochemistry 2019Quote: ... at which point IPTG (Gold Biotechnology; 1 mM final concentration) was added to induce expression ...
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bioRxiv - Microbiology 2019Quote: ... and 200 µM isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio). Unless otherwise indicated ...
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bioRxiv - Microbiology 2020Quote: ... Following overnight induction with isopropyl β-d-1-thiogalactopyranoside (Goldbio) at 25°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 µl 10 mM dNTP mix (Goldbio D-900-1), 46.3 mg DTT (Goldbio DTT10) ...
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bioRxiv - Microbiology 2022Quote: ... selected with 200 μg/ml hygromycin (GoldBio #H-270-1), and subcloned by serial dilution ...
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bioRxiv - Microbiology 2022Quote: ... coli: 200 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG, GoldBio), 0.1% L-arabinose (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... 1 mM 4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF, Gold Biotechnology), 1 mM benzamidine hydrochloride monohydrate (benzamidine ...
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bioRxiv - Biochemistry 2024Quote: ... and overnight expression was done by 1 mM IPTG (GoldBio) in 18 °C shaker ...
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bioRxiv - Molecular Biology 2023Quote: ... diluting 5X with storage buffer containing 1 mM DTT (GoldBio), and incubating with either 2.5 µl (∼25 units ...
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bioRxiv - Systems Biology 2023Quote: ... and 150 μg/mL Hygromycin (Gold Biotechnology, H-270-1) in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: 1 kb DNA Ladder (Gold Biotechnology cat. no. D010-500)
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DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: ... 0.02 mg/mL bovine pancreas DNAse (GoldBio D-301-1), 1 mg/mL lysozyme (EMD Millipore Corp ...
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bioRxiv - Biophysics 2022Quote: ... GadC expression was induced by adding 1 mM IPTG (Gold Biotechnology) and the temperature was dropped to 20°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 1.5μg ml-1 puromycin dihydrochloride (GoldBio, Cat. no. P-600). Recombinant C5a (human ...
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bioRxiv - Microbiology 2023Quote: ... and 100 uM isopropyl β-d-1-thiogalactopyranoside (IPTG, GoldBio, 221105I2481) and shaken aerobically at 37°C for 16 hours ...
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bioRxiv - Biophysics 2024Quote: ... Isopropyl-β-D-thiogalactoside (IPTG, 1 mM; Gold Biotechnology, cat I2481C50) was used for induction and cells were grown for another 15 hours at 25° C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were selected in 200 μg ml−1 Hygromycin B (Gold Biotechnology) and colonies were isolated and expanded ...
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bioRxiv - Biophysics 2022Quote: ... 200 rpm and induced with 1 mM IPTG (GoldBio, St.Louis, MO, USA). Cells were harvested 4 hours after induction when the optical density (OD600 ...
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bioRxiv - Cell Biology 2022Quote: ... containing ProBlock Gold™ Mammalian protease inhibitor cocktail (Goldbio, GB-331-1) and Benzonase (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then resuspended in 20 μl 1% low-melt agarose (GoldBio) in PBS at 40°C and loaded into the wells of preformed 1% agarose (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... isopropyl-β-D-1-thiogalactopyranoside (IPTG; final 0.1~1mM) (GOLDBIO, cat# 12481C50), or catalase 1μg/ml (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... 1 μM BirA and 10 mM ATP (Gold Biotech, #A-081-100) at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... sucrose (5% w/v) or isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio) at 1 mM or 2 mM ...
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bioRxiv - Biochemistry 2023Quote: ... and D-luciferin (0.5 mg ml-1) (GoldBio, Cat. no: LUCNA-1G). Cells were seeded in 96-well flat bottom white plate (Corning ...
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bioRxiv - Synthetic Biology 2023Quote: ... 40 μl of 0.1 M isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio) and 120 μl of 20 mg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal ...
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bioRxiv - Biochemistry 2023Quote: ... Isopropyl β-;D-1-thiogalactopyranoside (IPTG) (Cat # I2481C) was from Gold Biotechnology. Ni(II)-NTA resin (Cat # 30210 ...
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bioRxiv - Biochemistry 2022Quote: ... by addition of 400 μM Isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio) and lowering the incubator temperature to 20°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1% IGEPAL CA-630) supplemented with protease inhibitor cocktail (Goldbio, GB-331). Following incubation on ice for 15 minutes ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... 1 µg/mL leupeptin) containing 1x ProBlock™ Protease Inhibitor Cocktail −50 (GoldBio). Total concentration of protein in lysate was quantified using the Pierce™ BCA Protein Assay Kit measured at 562 nm and compared to a standard curve ...
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bioRxiv - Biochemistry 2020Quote: ... Expression was induced overnight with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio) and cultures were grown overnight at 18 °C ...
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bioRxiv - Immunology 2021Quote: ... and Luciferin at a 1:100 ratio supplied by (Gold Biotechnology LUCK-1G) when appropriate.
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bioRxiv - Microbiology 2022Quote: ... cells were induced with 50 μM Isopropyl β-d-1-thiogalactopyranoside (IPTG) (GoldBio). Expression was carried out at 8 °C and cells were collected at 20 hours after inoculation by centrifugation at 5000×g for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Triton X-100) freshly supplied with 1x ProBlock protease inhibitor cocktail (GoldBio), and 1 mM Phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Developmental Biology 2024Quote: ... in DMEM/F12 containing 1% fatty acid-free bovine serum albumin (Gold Biotechnology) in a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... at 1 mg/mL and elastase at 6 U/mL (E-240, Goldbio) for 1 h ...