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Citations for Gold Biotechnology :
51 - 100 of 294 citations for Mouse Anti Toxoplasma gondii SAG 1 protein 6207 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by adding lysis buffer containing 35 mM reduced glutathione (Gold Biotechnology). The elute fractions were run on a 4-12% NuPAGE Bis-Tris protein gel (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... along with 2.5 μl of a pre-stained protein ladder (#P008; GoldBio, St. Louis, MO), and separated on an SDS-polyacrylamide gel ...
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bioRxiv - Immunology 2020Quote: Supernatant containing RBD-Fc was purified over protein A agarose resin (Goldbio, Cat# P-400), cleaved with HRV3C protease (made in house ...
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bioRxiv - Immunology 2021Quote: ... All GST fusion proteins were purified using Glutathione Agarose 4B (Gold Biotechnology, #G-250-50), and eluted with HEPES buffered saline (HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... the GltPh proteins were reduced with 500 µM tris(2-carboxy-ethyl) phosphine (TCEP, Gold Biotechnology) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... CLEC18A-Fc fusion was purified from the supernatant with an Protein A agarose resin (Gold Biotechnology). Protein A beads in an ethanol solution were centrifuged at 150 x g for 5 min to remove the ethanol solution ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (GoldBio) was added to induce protein expression at 37 °C for 4 h ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The His6-tagged protein variants present in the supernatant were purified using Ni2+-NTA affinity chromatography (Goldbio) and dialyzed overnight at 4 ⁰C against 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology, Olivette, MO), eluted with 300mM imidazole ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... 120 µg protein was mixed with substrate containing 15mM ONPG (2-Nitrophenyl-β-D-galactopyranoside, Goldbio, #N27510), 5mM DTT ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant contains solubilized mitochondrial proteins and mixed with 5% G-250 (GoldBio, cat. C-460-5) and 1x NativePAGE sample buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Each mouse was intraperitoneally injected with 3 mg D-luciferin (Gold Biotechnology Inc., St. Louis, MO, USA) in 200 µl PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor-bearing mice were anesthetized by isoflurane inhalation and d-luciferin (Gold Biotechnology; 150mg/kg mouse weight) was administered by intraperitoneal (i.p. ...
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bioRxiv - Cell Biology 2022Quote: ... and rifampicin (GoldBio #R-120-1, 100 μg mL-1). Bacterial treatments for actin dynamics analysis were conducted following previously described methods36 ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg ml-1 pepstatin A (P-020-100, GoldBio), 100 μg ml−1 4-(2-Aminoethyl ...
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bioRxiv - Bioengineering 2022Quote: ... Protein expression was induced by the addition of 1mM isopro-pyl-L-thio-D-galactopyranoside (IPTG) (Gold Biotechnology) at 37°c and after 35 minutes ...
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bioRxiv - Biophysics 2023Quote: ... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Culture growth at 37 °C was monitored by measuring absorbance at 600 nm (A600) and protein expression was induced at A600 = ∼0.7 with 0.5 mM IPTG (GoldBio). The culture was grown at 18 °C for ∼15 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... 120 ug of protein was combined with substrate containing 15mM ONPG (2-Nitrophenyl-β-D-galactopyranoside, Goldbio, #N27510), 5mM DTT ...
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bioRxiv - Biochemistry 2024Quote: ... and induced with 1 mM Isopropyl b-d-1-thiogalactopyranoside (IPTG, Goldbio) overnight at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mM IPTG (Goldbio) was added and the cultures were set at 15 °C for 24 h (230 rpm) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM PMSF (GoldBio), 1X Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mM IPTG (GoldBio), 2 mg/ml L-arabinose (GoldBio) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM TCEP (GoldBio), 5% glycerol (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM luciferin (GOLDBIO) was applied onto leaves and the images were captured using Lumazone imaging system equipped with 2048B CCD camera (Roper).
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bioRxiv - Microbiology 2023Quote: ... CYE was supplemented with 10 µg mL-1 chloramphenicol for plasmid maintenance and legC4 expression was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; GoldBio) where indicated ...
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bioRxiv - Microbiology 2020Quote: ... The protein was eluted with 5 mL of elution buffer (20 mM TEA, 50 mM KCl, 500 mM imidazole (GoldBio), pH 7) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were harvested by centrifugation (6000xg for 20 min) and lysed with B-PER Bacterial Protein Extraction Reagent (Thermo Fischer) supplemented with DNAse I (GoldBio). Lysate was incubated at 60°C for 20 min and cleared by centrifugation at 14,000xg for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila was induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) (GoldBio).
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bioRxiv - Synthetic Biology 2021Quote: ... We used 1 mM of Isopropyl β-D-1-thiogalactopyranoside (IPTG, Gold Biotechnology) for the induction of FnCpf1.
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/mL leupeptin) containing 1× ProBlock™ Protease Inhibitor Cocktail-50 (GoldBio) and processed as previously described[17] ...
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bioRxiv - Cancer Biology 2022Quote: ... The beads bound with biotinylated cell surface proteins were resuspended in 50 mM Tris (pH 8.5) + 4 M urea + 10 mM TCEP (Gold Biotechnology, TCEP10) and 20 mm IAA (VWR ...
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bioRxiv - Synthetic Biology 2021Quote: ... cultures were induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Gold Biotechnology) and then kept at 25 °C with shaking at 225 rpm for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... (1 mg/ml, Gold Biotechnology). SC-Leu+MPA media is minimal media of SC-Leu supplemented with mycophenolic acid (20 ug/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM AEBSF (Gold Biotechnology), 1 mM Benzamidine (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 mM IPTG (GoldBio I2481C), 400 μM erythromycin ...
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bioRxiv - Plant Biology 2021Quote: ... and 1 mM DTT (GoldBio). Protein concentration was measured using the Bradford method (Bradford ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM IPTG (I2481, GoldBio) was added and the cultures were shifted to 18°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM IPTG (Gold Biotechnology) was added to induce the protein expression ...
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bioRxiv - Biophysics 2023Quote: ... 1% of DDM detergent (GoldBio) was added to the lysed cell and the cells was incubated for 1 h at 4°C to extract the membrane protein ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mM IPTG (GoldBio, I2481C), 1mM m-toluic acid (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM X-Gluc (GoldBio), and 0.01% Silwet L-77) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM dithiothreitol (GoldBio, DTT25), 5 mM NTP Set (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM IPTG (GoldBio I2481C), 1mM m-Toluic acid ...
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bioRxiv - Biophysics 2024Quote: ... the temperature was reduced to 20 °C and protein expression was induced with the addition of IPTG (GoldBio, St. Louis, MO, USA) to a final concentration of 1 mM ...