Labshake search
Citations for BMG Labtech :
1101 - 1150 of 1159 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... mScarlet-I (target) and Venus (reference) were excited at 560 ± 10 nm and 497 ± 15 nm in a CLARIOstar plate reader (BMG Labtech, Ortenberg, Germany). Emission was recorded at 595 ± 10 nm (mScarlet-I ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were excited at 370–496 nm with a step width of 1 nm and the emission collected at 530±5 nm using a CLARIOstar plate reader (BMG Labtech, Offenburg, Germany). To achieve complete reduction or oxidation of the sensor ...
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bioRxiv - Biochemistry 2020Quote: QF peptide cleavage assays were conducted in 96-well black microtiter plates (Scientific Laboratories Supplies Ltd, Wilford, UK) using a Fluostar Omega microplate reader (BMG Labtech, Aylesbury, UK). The activity of ADAMTS-4 and −5 was monitored for 2 h using the fluorescent peptide substrates fluorescein 5(6)-carbonyl -Ala-Glu-Leu-Asn-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-N,N,N0,N0-tetramethyl-6-carboxyrhodamine-NH2 (FAM-AE~LQGRPISIAK-TAMRA ...
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bioRxiv - Biophysics 2021Quote: ... The assay was then initiated by placing the microplate at 37 °C under quiescent conditions in a plate reader (FLUOstar Omega, BMG Labtech, Aylesbury, UK). The ThT fluorescence was measured through the bottom of the plate with a 440 nm excitation filter and a 480 nm emission filter ...
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bioRxiv - Systems Biology 2021Quote: ... Plates were incubated for 5 min and centrifuged for 5 min (173 × g) before reading luminescence with PHERAstar FS multimode plate reader (BMG Labtech, Ortenberg, Germany).
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bioRxiv - Cell Biology 2021Quote: ... The two components were combined 1:1 in a final volume of 20 µL in and the reaction monitored in a Pherastar plate reader (BMG LABTECH GmbH, Germany) using excitation at 485 nm (±10 nm ...
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bioRxiv - Microbiology 2020Quote: ... plates were read to quantify SYBR Green I® fluorescence intensity in each well by a PHERAstar® FSX microplate reader (BMG Labtech) with excitation and emission wavelengths of 485 and 520nm respectively ...
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bioRxiv - Neuroscience 2022Quote: Ligand-binding activity of cell lines co-expressing Orco and ORs was tested via a calcium fluorescence assay using a CLARIOstar Omega plate reader (BMG Labtech, Ortenberg, Germany) according to previously described protocols (Andersson et al ...
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bioRxiv - Physiology 2022Quote: ... cells were incubated with Caspase-Glo® 3/7 reagent at room temperature before recording luminescence with a POLASTAR plate reader (BMG Labtech, Germany).
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bioRxiv - Biophysics 2022Quote: ... emission 535 nm) was measured in parallel and perpendicular to the excitation plane on a PheraStar FS plate reader (BMG Labtech GmbH, Germany) and polarization was calculated ...
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bioRxiv - Biophysics 2022Quote: ... emission 535 nm) was measured in parallel and perpendicular to the excitation plane on a PheraStar FS plate reader (BMG Labtech GmbH, Germany). The measured intensities were reduced by the values of only AcmJRL in TBS ...
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bioRxiv - Immunology 2022Quote: ... Fluorescence intensity at 460 and 530 nm emission and 405 nm excitation was measured by an PHERAstar plate reader (BMG LABTECH, Cary, NC). Data were represented as the ratio of the emission wavelengths (460nm/530nm).
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bioRxiv - Bioengineering 2022Quote: ... Afterwards the supernatants were transferred to 96 well plates and the absorbance was measured at 580 nm in a microplate reader (FLUOstar, BMG LabTech, Offenburg, Germany). A standard curve was prepared by measuring the absorbance for a series of trypan blue solutions at different concentrations ...
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bioRxiv - Synthetic Biology 2020Quote: ... The solution underwent centrifugation at 4000 r.p.m for 20 minutes and the top hexane layer was transferred to a shallow 96-well plate and absorbance at 530 nm was determined (BMG LABTECH POLARstar Omega).
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bioRxiv - Microbiology 2021Quote: ... Concentration and quality of extracted DNA was determined with spectrometry absorbance between 230–280 nm detected by a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies) and OPTIMA fluorescence plate reader (BMG LABTECH, Jena, Germany). Fungal DNA were subsequently amplified by PCR targeting the internal transcribed spacer (ITS2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were transferred to 384-well plate and emission (530 nm) was measured with excitation wavelengths of 400 and 482 nm using a Clariostar plate reader (multi-chromatic) (BMG Labtech, Ortenberg, Germany). The calculated ratio of 400/482 nm was then plotted using GraphPad Prism 8.0.2 software ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The reaction was run at 30°C for 6 h followed by inactivation at 65°C for 5 min and was monitored by detection of SYTO13 fluorescence every 15 minutes using a FLUOstar® Omega plate reader (BMG Labtech, Germany). The single amplified genome (SAG ...
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bioRxiv - Immunology 2021Quote: ... The colorimetric reaction was then terminated with 100 µL of 0.3 M sulfuric acid and the absorbance of the yellow resultant product was measured for each sample at 450nm using a plate reader (FLUOstar Omega, BMG Labtech, Ortenberg Germany). Per Bethyl Laboratories protocol ...
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bioRxiv - Immunology 2021Quote: ... An excitation filter of 485nm and an emission filter of 520nm was used to quantitate mean fluorescent intensity using a fluorescent plate reader (FLUOstar Omega, BMG Labtech, Ortenberg, Germany).
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bioRxiv - Bioengineering 2022Quote: ... The converted fluorescent products were photometrically quantified at an excitation wavelength of 544 nm and an emission wavelength of 590 nm using a plate reader (FLUOstar, BMG LabTech, Offenburg, Germany). Cells without films were used as negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... Time-resolved fluorescent measurements were carried out at an excitation wavelength of 340 nm and an emission wavelength of 614 nm on a BMG POLARstar plate reader (BMG Labtech, Melbourne, Australia). Binding was performed in at least three independent experiments with triplicate determinations within each assay ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The microplate with cROS and mROS samples was analysed for ROS formation using a FLUOstar Optima fluorescence plate reader (BMG Labtech, Ortenberg, Germany) with an emission wavelength of 535 nm and excitation wavelength of 485 nm ...
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bioRxiv - Microbiology 2022Quote: ... plates were read to quantify SYBR Green I® fluorescence intensity in each well by a PHERAstar® FSX microplate reader (BMG Labtech) or the CLARIOstar microplate reader (BMG Labtech ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were incubated with 100 μl Caspase-Glo® 3/7 reagent at room temperature for 1 h before recording luminescence with a POLASTAR plate reader (BMG Labtech, Germany).
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bioRxiv - Molecular Biology 2022Quote: ... Luciferase activity in cells was measured using Renilla Luciferase Glow Assay Kit (Pierce Biotechnology, PA, USA) and the CLARIOstar Plus plate reader (BMG LABTECH, NC, USA) at 24 h later.
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated with the antibodies for 2 h at room temperature and then read using a PheraStar FS multi-mode plate reader (BMG Labtech, Ortenberg, Germany). Insulin concentrations (in ng/mL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fluorescence resonance energy transfer (FRET) measurements were measured in real-time or 90 min after ligand stimulation in a CLARIOstar Plus plate reader (BMG Labtech; Ortenberg, Germany) using excitation at 430-15 nm and emission at 480-20 and 530-20 nm.
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bioRxiv - Plant Biology 2023Quote: Interaction of GRXs with roGFP2 was analyzed in vitro by ratiometric time-course measurements on a fluorescence plate reader (CLARIOstar, BMG Labtech, Ortenberg, Germany) with excitation at 400 ± 5 nm and 480 ± 5 nm and detection of emitted light at 520 ± 5 nm ...
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bioRxiv - Bioengineering 2023Quote: ... then left to cool at room temperature for 5 min before absorbance was measured at 562 nm with a plate reader (CLARIOstarPlus, BMG Labtech, Ortenberg, Germany). The final concentration of purified protein was 1076 μg/mL ...
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bioRxiv - Synthetic Biology 2024Quote: Samples collected as described in the last section were washed with 10mM HEPES buffer and loaded onto a plate reader (BMG Labtech, Ortenberg, Germany) for fluorescence measurement at 470/515 nm (Ex/Em) ...
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bioRxiv - Biochemistry 2024Quote: The multiparametric real-time measurements were conducted with a CLARIOstar multiwell plate-reader equipped with an atmospheric control unit (BMG Labtech, Ortenberg, Germany). Leaf disc samples were cut from approximately five-week-old Arabidopsis rosettes with a leaf disc cork borer as described previously [23] ...
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bioRxiv - Biochemistry 2022Quote: ... The concentration of the pNP enzymatic product was determined by measuring A405 nm using a 96-well plate reader (BMG Labtech, Ortenberg, Germany) using a pNP standard curve (0 to 140 µM pNP) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were treated with 5 μM coelenterazine-H (NanoLightTM Technology) for 5 min and analyzed with a PHERAstar plate reader (BMG LABTECH, Cary, NC). BRET signals were determined by calculating the ratio of the light emitted by mVenus (535/30 nm ...
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bioRxiv - Immunology 2023Quote: ... Relative luciferase units (RLU) present in cell lysates was then assessed with BrightGlo using a FLUOstar Omega plate reader (BMG Labtech, Cary, NC) with the MARS software ...
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bioRxiv - Genetics 2023Quote: ... DNA concentration was measured using Qubit® dsDNA HS Assay Kit (life technologies) and a fluorescence plate reader (FLUOstar Omega, BMG Labtech, Germany). Enzymatic conversion was performed using the NEBNext Enzymatic Methyl-seq Conversion Module (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... Cells were grown in either a CLARIOstar (all growth assays except for Fig. S2) or Fluostar plate reader (for Fig. S2, BMG LABTECH, Ortenberg, Germany) at 30°C and 700 rpm orbital shaking and OD600 was measured every 5 minutes.
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bioRxiv - Plant Biology 2024Quote: ... The samples were loaded onto a black 96-well plate and SOSG fluorescence was measured before and after 10 min of HL illumination in a fluorescence plate reader (ClarioStar 500, BMG Labtech, Ortenberg, Germany) with excitation at 488 nm and an emission range of 530-600nm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Lysates were transferred to an opaque multi-well plate and luminescent signals were quantified at 540 nm using a POLARstar Omega microplate reader (BMG Labtech, Ortenberg, Germany). Cell viability in treated cells was expressed as the percentage of the luminescent values measured in untreated cells ...
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bioRxiv - Immunology 2024Quote: ... Fluorescence was measured with an excitation wavelength of 330 nm and emission wavelength of 390 nm using a CLARIOstar Plus plate reader (BMG Labtech, Ortenburg, Germany). Fluorescence intensity from each well was averaged across 16 locations in the well and was measured approximately once every 1-2 minutes (dependent upon total cycle length ...
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bioRxiv - Microbiology 2024Quote: ... The crystal violet stain in each well was resolubilised in 125 μL 30% v/v acetic acid solution and absorbance readings were taken using a CLARIOstar Plus plate reader (BMG Labtech, Ortenberg, DE) at 570 nm (after 5 s double-orbital shaking at 500 rpm) ...
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bioRxiv - Biophysics 2024Quote: ... and the increase in ThT fluorescence intensity at 480 nm was measured every 33 minutes by a POLAR Star Omega plate reader (BMG Labtech, Cary, NC). At least three replicates of the ThT assays were recorded for each sample ...
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bioRxiv - Biophysics 2024Quote: ... and the increase in ThT fluorescence intensity at 480 nm was measured every 33 minutes by a POLAR Star Omega plate reader (BMG Labtech, Cary, NC). At least three replicates of the ThT assays were recorded for each sample ...
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bioRxiv - Cell Biology 2020Quote: ... Plates were read every hour for 7 h at an excitation/ emission spectra of 280 nm/ 350 nm on a SPECTROstar-nano plate reader (BMG LABTECH Ltd, Bucks, UK).
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bioRxiv - Biochemistry 2021Quote: ... Color intensity was read at 450 nm with a reference filter of 570 nm in an ELISA plate reader (FLUOstar, BMG LABTECH, Inc., Cary, NC). IL-6 concentrations were extrapolated from a standard curve plotted using known concentrations of IL-6 ...
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bioRxiv - Microbiology 2022Quote: ... 200 μl of the supernatant was transferred to a black 96-well plate to measure the fluorescence using a BMG FLUOstar Omega Microplate Reader (BMG Labtech Inc. Cary, NC). From sample ...
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bioRxiv - Genetics 2022Quote: ... Protein concentration of the lysates was measured using the BCA assay (Pierce, cat. no. 23225) and filtered lysates were analyzed using a plate reader (BMG Labtech CLARIOstar microplate reader) at OD450nm.
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bioRxiv - Synthetic Biology 2021Quote: ... After a double orbital shake for 10 seconds at 300 rpm, fluorescence was measured (excitation 470 nm, emission 515 nm) using a CLARIOstar plate reader (BMG Labtech Gmbh, Ortenberg, Germany).
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... Proteins were suspended in 50mM ammonium bicarbonate in 8M urea and then quantified (Bio-Rad DC Protein Assay, BMG Labtech POLARstar Omega plate reader). To reduce disulphide bonds ...
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bioRxiv - Microbiology 2024Quote: ... was used with growth measured via absorbance (optical density at 600 nm wavelength) at 30°C for 48 hours in a plate reader (CLARIOstar, BMG LABTECH GmbH, Ortenberg, Germany). Isolated P ...
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bioRxiv - Biochemistry 2024Quote: ... ThT fluorescence was recorded every 5 minutes for 16 h on a FLUOstar Omega plate-reader (BMG LABTECH, excitation: 440 nm, emission: 482 nm). Background ThT fluorescence of chaperones and buffer was subtracted and ThT fluorescence was normalised to the fluorescence intensity of the first time point (t = 0 min) ...