Labshake search
Citations for BMG Labtech :
1051 - 1100 of 1159 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The enzymatic reaction was stopped after 5-10 min using 50 µL 1 M H2SO4 and the absorbance was read at 450 nm using a CLARIOstar plate reader (BMG Labtech).
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bioRxiv - Biochemistry 2024Quote: ... The ThT fluorescence signal was monitored as a function of time using a plate reader (FluoStar Omega, BMG Labtech, Offenburg, Germany) under quiescent conditions at 37 °C with excitation at 440 nm and emission at 480 nm.
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bioRxiv - Biophysics 2024Quote: ... Thirty μl aliquots were dispensed in a 384-well plate (company) that was sealed and placed in a Fluostar Omega (BMG Labtech) plate reader ...
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bioRxiv - Microbiology 2024Quote: ... The attraction of cells towards the ICZ with and without arabinose added was quantified by transferring the liquid from the capillaries to a 96-well plate and measuring fluorescence using a FLUOstar Omega reader (BMG LABTECH) at the excitation and emission maxima of 497/509 nm ...
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bioRxiv - Physiology 2024Quote: ... The protein content of lysates was measured using the DC Protein Assay kit with a Clariostar plate reader from BMG Labtech. Equal amount of protein from tissue was resolved on 10-15% SDS-PAGE gels and then transferred to PVDF membranes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... ThT fluorescence was measured to evaluate the development of Tau fibrils over time using a fluorescence plate reader (Fluostar Optima, BMG labtech) with 384-wells flat-bottom black plates (final volume in the wells of 40 μL) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then lysed using luciferin-containing buffer79 and quantitation of luciferase signal was obtained using a PHERAstar FSX plate reader (BMG LabTech). Percentage of infection was determined by calculating the difference in luminescence between test wells (cells with virus and antibody ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of Ub-Rho solution was transferred to the 384-well plate and quickly centrifuged before reading on the CLARIOstar (BMG Labtech). Fluorescence was measured for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... measuring changing absorbance at 486 nm over 10 mins (Δε 486 = 20,500 M-1 cm-1) using a Clariostar plate reader (BMG LabTech)49 ...
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bioRxiv - Microbiology 2024Quote: ... The manufacturer’s instructions for a microplate assay were followed with a 30 min reaction time before reading on the CLARIOstar plate reader (BMG LABTECH).
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bioRxiv - Cancer Biology 2024Quote: ... Results were normalized to cell number obtained by fluorescence intensity of Hoechst 33342 correlated with nucleus count performed at 460 nm on a POLARstar Omega plate reader (BMG Labtech).
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bioRxiv - Biophysics 2024Quote: ... Lysates were transferred to an opaque multi-well plate and luminescent signals were quantified at 540 nm using the Polarstar Omega microplate reader (BMG Labtech). Cell viabilities in treated cells were expressed as the percentage of the luminescent values obtained in untreated cells ...
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bioRxiv - Biophysics 2024Quote: ... BRET was measured at multiple serially diluted concentrations of either imatinib or dasatinib and 650 nm (acceptor emission) in a PHERAstar plate reader (BMG Labtech). Background-corrected BRET ratios (610 nm/450 nm ...
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bioRxiv - Cell Biology 2024Quote: ... Total and secreted insulin samples from islets and cells were stored at −20°C until quantification using an Insulin Ultra-Sensitive HTRF kit (62IN2PEG, Revvity) in a PHERAstar plate reader (BMG Labtech), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence of the SOX-peptide after 360 nm excitation was measured at 487 nm emission with a PHERAstar plate reader (BMG Labtech). The IC50 values were calculated from a non-linear regression of the log[inhibitor] vs ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescence was measured in kinetic mode at room temperature for 60 min with 2-minute intervals in a CLARIOstar plate reader (BMG LABTECH).
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bioRxiv - Cell Biology 2024Quote: ... BRET emissions using the short-wavelength filter at 475 nm and long wavelength filter 535 nm were measured in 10 repeat cycles using a LUMIstarOPTIMA plate reader (BMG Labtech). Fluorescence of Venus-tagged receptor was measured in duplicate after excitation at 485 nm and emission at 535 nm.
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bioRxiv - Cell Biology 2020Quote: ... Light emitted as a result of the reaction between D-luciferin and luciferase was detected using a 96 well micro-injector plate reader (POLARstar OPTIMA, BMG LABTECH GmBH). To normalize the measured ATP release to cell protein ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitation of secreted factors proceeded exactly as per manufacturer’s instructions with the absorbance value determined on a PHERAstar FS plate reader (BMG Labtech, Ortenberg, Germany) at 450 nm ...
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bioRxiv - Genetics 2021Quote: ... Absorbance (OD600) of each well was measured on a SPECTROstar Omega microplate reader equipped with a plate stacker (BMG Labtech, Ortenberg, Germany) over a period of 2-3 days at ambient temperature (∼25 C ...
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bioRxiv - Genomics 2021Quote: ... was added to stop the color development and read immediately at 450nm (yellow color) using FLUOstar® Omega Plate Reader by BMG LABTECH.
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bioRxiv - Biochemistry 2020Quote: ... covered with a thermal seal film (EXCEL Scientific, USA) and incubated in a plate reader (CLARIOstar or FLUOstar Omega, BMG LABTECH, Germany), at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... and the fluorescence emission at 480 nm was measured every five minutes in a 96-well plate in a FLUOstar Omega microplate reader (BMG LABTECH, Germany). The sample volume in each well was 35 μl ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were started by addition of dansylated lipid II to a final concentration of 10 µM and monitored by following the decrease in fluorescence over 60 min at 37°C using a FLUOstar OPTIMA plate reader (BMG Labtech, Germany) with excitation at 330 nm and emission at 520 nm ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were started by the addition of lipid II at 12 µM and monitored by measuring fluorescence over a period of 60 min (or 90 min for PBP1BPa liposomes) at 37°C using a Clariostar plate reader (BMG Labtech, Germany), with emission measurements at 590 nm and 680 nm after excitation at 522 nm ...
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bioRxiv - Microbiology 2021Quote: ... and HgCl2-preserved and frozen (−20°C) subsamples in the home lab after thawing using a microtiter plate reader (FLUOstar Optima, BMG Labtech, Germany) following established procedures for N oxides (NOx)34 ...
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bioRxiv - Biochemistry 2022Quote: ... After addition of substrate reaction was measured at excitation/emission wavelength 340/420 nm on a plate reader (Clariostar, BMG Labtech, Germany). For estimation of first order rate constants of kcat/Km substrate concentrations from 0.8 to 83 μM were used ...
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bioRxiv - Biochemistry 2022Quote: ... was loaded into a 96 well full-area plate (non-binding, clear bottomed) and incubated at 37 °C for ∼ 140 h in a CLARIOstar Plus microplate reader (BMG Labtech, Germany). Aggregation was promoted through linear shaking (300 rpm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Lumi4™-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using a CLARIOstar plate reader (BMG Labtech). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by ACh.
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bioRxiv - Cancer Biology 2020Quote: ... The plate was incubated at 37°C for 2 hours before the absorbance is measured at 490nm using a POLARstar OPTIMA plate reader (BMG Labtech, UK). Readings were blank corrected by subtracting cell free controls and data normalised to untreated control.
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bioRxiv - Biochemistry 2020Quote: ... and β-NADH absorbance at 340 nm (A340) was measured over time at 25°C with a Clariostar plate reader (BMG Labtech). Linear regression analysis was performed with Prism 8 (GraphPad ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescent intensity (parallel and perpendicular polarization) of each reaction were measured using a ClarioStar Plus FP plate reader (BMG Labtech, Ortenberg, Germany) and fluorescent anisotropy values of each protein titration were calculated ...
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bioRxiv - Biochemistry 2021Quote: ... Then the fluorescence intensity (λexcitation 560 ± 5 nm and λemission 590 ± 5 nm) was measured using the FLUOstar OPTIMA plate reader (BMG Labtech, Germany). The obtained fluorescence intensity data were normalized to vehicle control corresponding to 100 % sphere formation and the signal after 100 μM benzethonium chloride (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... and RNA purity was evaluated by 260/230 nm and 260/280 nm ratios using a CLARIOstar plate reader (BMG LABTECH, Germany). cDNA was synthesised from 2 μg RNA using the GoScriptTM Reverse Transcription kit (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... Cell viability was determined upon addition of 25 µl CellTiter-Glo® reagent in accordance with manufacturer’s instructions and luminescence was measured using a ClarioSTAR Plus plate reader (BMG Labtech, Germany).
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bioRxiv - Microbiology 2024Quote: ... Absorbance at 450Dnm (mAb binding intensities) was measured with a SPECTROstar® Nano absorbance plate reader using the MARS software (BMG Labtech). Fucan from Laminaria (Glycomix ...
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bioRxiv - Microbiology 2023Quote: ... The cells were pelleted and the remaining cytochrome C in the supernatant was measured spectrophotometrically at OD410 using a FLUOstar OMEGA plate reader (BMG Labtech, UK).
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bioRxiv - Neuroscience 2023Quote: ... ThT fluorescence was monitored with bottom optics at λex = 448 nm and λem = 482 nm in a fluorescence plate reader with orbital averaging on 3 mm (Clariostar or Polarstar Optima, BMG labtech, GE). Prior to measurement start ...
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bioRxiv - Neuroscience 2023Quote: ... The MTT formazan product was quantified by measuring the absorbance at 570 nm corrected by subtraction of the absorbance at 660 nm in a FluoroStar Optima plate reader (BMG labtech, GE). All results were normalized to untreated cells grown in medium only ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence per well was measured in a 4×4 matrix (1400 gain) using a FLUOstar OPTIMA plate reader (BMG Labtech, Ortenberg, Germany) with emission filters at 485 nm and an excitation filter at 520 nm ...
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bioRxiv - Biochemistry 2023Quote: ... which converts resazurin into resorufin (excitation 550 nm, emission 585 nm) that was quantitatively analyzed by plate reader (CLARIOstar Plus, BMG Labtech, German).
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bioRxiv - Biochemistry 2023Quote: ... which converts resazurin into resorufin (excitation 550 nm, emission 585 nm) that was quantitatively analyzed by plate reader (CLARIOstar Plus, BMG Labtech, German). The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225 ...
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bioRxiv - Biophysics 2023Quote: A calibration curve was generated to correct raw absorbance readings collected outside the linear range of the plate-reader (POLARstar Omega, BMG Labtech, Germany) used in growth assays ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.5 s integration time and 1 min intervals at 37 °C using a CLARIOstar® Plus Multimode Plate Reader (BMG Labtech, Germany). The final volume in each well was 125 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... OD600 measurements were taken every 5 minutes with 700 rpm orbital shaking between time-points using a CLARIOstar® plate reader (BMG Labtech). Growth curves analysis was performed using an adaptation of the R package Growthcurver (Sprouffske and Wagner ...
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bioRxiv - Plant Biology 2024Quote: Yeast microculture growth for all solute transport assays was monitored in 96-well plate using a SPECTROStar nano absorbance microplate reader (BMG Labtech, Germany) at 10–20-minute intervals over 42 to 60 hours ...
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bioRxiv - Plant Biology 2024Quote: ... was added prior to quantifying luminescence as previously described (Cazzonelli and Velten, 2006) using the CLARIOstar® Plus plate reader (BMG LABTECH). Luminescence imaging of whole plants was performed by incubating them in 0.5 mM luciferin for 10 minutes prior to imaging light emission using a CCD camera (Model DV435 ...
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bioRxiv - Biochemistry 2024Quote: ... Quenching due to high fluorophore density was ruled out by confirming the absence of an increase of fluorescence signal upon denaturing of the fibrils in 4M GnHCl measured in CLARIOstar plate-reader (BMG LABTECH, 555nm). 60% AF-555-labeled XG fibrils and AF-555 labelled α-syn monomers are used as a control for fluorophore quenching positive and negative samples respectively.
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bioRxiv - Bioengineering 2024Quote: ... 100 uL of a 1 M HCl solution are added to stop the reaction before reading the plates with an absorbance reader at 450 nm (Fluostar OPTIMA, BMG LABTECH, France). The intensity of the signal was directly correlated with the ability of the compound to be recognized by the lectin.
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bioRxiv - Bioengineering 2024Quote: ... the stop solution was added to each well and the plates luminous activity was measured at 450 nm with a reference wavelength of 630 nm using a FLUOstart Omega plate reader (BMG LabTech, Germany). Plain tissue culture media which was incubated for 24 hours at 37°C was employed as the negative control ...