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Citations for Cambridge Isotope Labs :
201 - 250 of 277 citations for DL Lysine 2 15N DiHCl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Two HeLa cultures were grown in SILAC in RPMI(-)Arg(-)Lys supplemented with 10% dialyzed FBS and either with 100 ng/ml of 13C Lysine plus 30 ng/ml of 13C Arginine (Cambridge isotope Laboratories) for heavy labelling ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µL supernatant was added 40 µL solution of stable isotope-labeled internal standards (phenylalanine-D5, tyrosine-D4, valine-D8, methionine-D3, lysine-13C6-15N2, and arginine 13C6-15N4; Cambridge Isotope Laboratories) and 180 µL solvent (water with 0.2% heptafluorobutyric acid) ...
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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bioRxiv - Cell Biology 2023Quote: ... and either “Heavy” amino acids (50 μg/ml 13C6, 15N4 Arginine HCl and 100 μg/ml 13C6, 15N2 Lysine 2HCl; Cambridge Isotope Laboratories), or the equivalent “Light” amino acids ...
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bioRxiv - Microbiology 2024Quote: Fecal and plasma samples were diluted in 18 volumes of ice-cold 2:2:1 methanol/acetonitrile/water containing a mixture of internal standards (D4-citric acid, D4-succinic acid, D8-valine, and U13C-labeled glutamine, glutamic acid, lysine, methionine, serine, and tryptophan; Cambridge Isotope Laboratories), where the 1-part water was composed of sample volume + water ...
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bioRxiv - Systems Biology 2024Quote: ... the medium was replaced with pre-warmed DMEM for SILAC medium (Fischer Scientific) supplemented with heavy L-lysine and L-arginine (Cambridge Isotope Laboratories) and 10% dialyzed FBS (ThermoFischer Scientific).
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bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... 1,213C,2- or U13C6-glucose (2 g/l, 99%, Cambridge Isotope Laboratories) and incubated over night before treatment ...
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bioRxiv - Cell Biology 2021Quote: ... and Y858 (as Y859, but cdc55Δ ) cells were grown in free-methionine minimum media containing 13C6-lysine and -arginine (heavy, Cambridge Isotope Laboratories Inc., US) or unmodified arginine and lysine (light) ...
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bioRxiv - Cell Biology 2023Quote: ... L-LYSINE:2HCL (13C6, 99%;15N2, 99%) and stable isotope-labeled (heavy) L-ARGININE:HCL (13C6, 99%; 15N4, 99%) (Cambridge Isotope Labs, Inc. Andover, MA). Light SILAC medium was generated by adding unlabeled L-LYSINE:2HCL (final concentration 146.0 mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... and L-Lysine (0.2 mM) were replaced with the non-radioactive isotopic forms Arg-10 (13C6 H14 15N4O2; Cambridge Isotope Laboratories, cat. no. CNLM-539-H-PK) and Lys-8 (13C6 H14 15N2O2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 g 13C-glucose (Cambridge isotope). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... [13C4]-2-oxoglutarate ([13C4]-2OG) (Cambridge Isotope Laboratories), was added during metabolite extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM [U13C]-L-glutamine (Cambridge Isotope Laboratories), 10mM [1,2 -13C]-D-glucose (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally with AZD 5 mg/kg, 2 h prior to intravenous tail vein injection with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories). Intravenous injections were performed as a bolus over 20 s ...
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bioRxiv - Molecular Biology 2022Quote: ... [2-13C]glycine (Cambridge Isotope Laboratories, CLM-136-PK). All cell culture experiments were seeded with a starting cell density of 100,000 cells per mL and cultured for 2 days in the specified culture media ...
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bioRxiv - Molecular Biology 2022Quote: ... [2-13C]serine (Cambridge Isotope Laboratories, CLM-2013-PK), [2-13C]glycine (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... Ethyl [U-13C]2-hydroxybutyrate (Cambridge Isotope Laboratories, Inc.) approximated 2-hydroxybutyrate and 2-aminobutyrate signals ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended into 1 mL of ice-cold 2:2:1 MeOH: ACN: H2O (v/v/v) containing 10 μM stable isotope-labeled canonical amino acid mix (Cambridge Isotope Laboratories, Inc) as internal standards ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were prepared using 500uL of sample mixed with 500uL of an NMR buffer containing a chemical shift reference and analytical standard (TSP-2, 2, 3, 3-d4 (D,98% Sodium-3-Trimethylsilyl-Propionate, Cambridge Isotope Laboratories, Inc.). The NMR buffer was prepared with Mono- and Dibasic Potassium Phosphate at 0.5M concentration and a pH of 7 ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 g/L U-13C6-Glucose (Cambridge Isotope Laboratories) as the sole nitrogen and carbon sources ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 g / L 13C6–D-glucose (Cambridge Isotope Laboratories) and sodium bicarbonate (Sigma Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... or 2 mM 13C5-glutamine (Cambridge Isotope Laboratories, CLM-1822-H) was added to respective dropout media ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10 mM 13C6-glucose (Cambridge Isotope Laboratories, CLM-1396-2) and dialyzed FBS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... [U-13C]-18:2(n-6) (Cambridge Isotope Laboratories, CLM-6855-PK) was supplemented as bovine serum albumin-fatty acid (BSA-FA ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in RPMI containing 2 mM 13C5,15N2-glutamine (Cambridge Isotope Labs) as the sole glutamine source for 2 hours before metabolites were extracted and quantified as described above with the exception that no internal standards were added to the extraction buffer ...
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bioRxiv - Biochemistry 2024Quote: ... or 2 mM 13C3-pyruvate (CLM-2440-PK, Cambridge Isotope Laboratories, MA, USA). Samples were incubated at 37°C for 30 minutes ...
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bioRxiv - Systems Biology 2021Quote: ... 13C-[6C]-labeled 2-deoxy-D-glucose (Cambridge Isotope Laboratories, Inc., 12 Ci/mmol); [1-3H] 2-Deoxy-D-glucose (ViTrax Radiochemicals) ...
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bioRxiv - Microbiology 2024Quote: ... and 2 g/L [13C]glucose or 0.5 g/L 15NH4Cl (Cambridge Isotope Laboratories). E ...
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bioRxiv - Cancer Biology 2024Quote: ... and isotope-labeled internal standards [700 pmol 2’-deoxycytidine-15N3 (Cambridge Isotope Laboratories, Inc.); 1.75 pmol 5-methyl-2’-deoxy cytidine-d3 (Toronto Research Chemicals) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantification was performed using the internal standard 2-hydroxyglutaric acid-D3 from Cambridge Isotope Laboratories (Tewksbury ...
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bioRxiv - Plant Biology 2024Quote: ... (v/v 2:1) with 10 μM 13C(6)-tyrosine (Cambridge Isotope, CLM-1542-0.25) as an internal recovery standard ...
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bioRxiv - Cell Biology 2023Quote: ... Mice were then injected with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories) via intravenous tail vein ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled media refers to EMM with 2% D-Glucose (U-13C6) (Cambridge Isotope Laboratories, Inc.). Once cells came into contact with labelled media ...
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bioRxiv - Biophysics 2023Quote: ... in dry acetonitrile (10 ml) was added 2-bromoethanol-1,1,2,2-d4 (240 μl, Cambridge Isotope Laboratories) under argon ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a 2 mM solution of isotopically labeled sodium bicarbonate (NaH13CO3; Cambridge Isotope Laboratories Inc., Tewksbury, MA) was added to a batch culture of R ...
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bioRxiv - Biochemistry 2024Quote: ... and 100 mg/L 2-keto-3-[D]-[13CH3,12CD3]- isovalerate (Cambridge Isotope Laboratories, Inc. CDLM-7317) to enable selective labeling of ILV methyl groups ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/l of U-[2H,13C] D-glucose and 1 g/l 15NH4Cl (Cambridge Isotope Laboratories). For production of U-[2H ...
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bioRxiv - Microbiology 2023Quote: ... Fractions 1–2 were prepared in 100% deuterated H2O (D2O; Cambridge Isotope Laboratories; referenced at 4.39 ppm) and Fractions 3–4 in 10% CD3OD ...
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bioRxiv - Biophysics 2022Quote: ... and 2 g/L U-[2H] glucose (Sigma-Aldrich, St. Louis, MO/Cambridge Isotope Laboratories, Inc., Tewksbury, MA). Cells were grown up to an OD600 of 0.65 at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µl of 2× kinase buffer containing 80 µM O18 ATP (OLM-7858-PK, Cambridge Isotope laboratories, Inc) and 1 µg of bacterial purified human PIP4Kα-GST was added and the reaction was incubated at 30 °C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... and 2 ng of [13C6]-IAA was added as an internal standard (Cambridge Isotope Laboratories, Andover, MA, USA). The sample was extracted for 2h at 4°C under continuous shaking ...
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bioRxiv - Plant Biology 2023Quote: RG-II (1-2 mg) was dissolved in D2O (0.2 mL, 99.9%; Cambridge Isotope Laboratories, Tewksbury, MA, USA) and placed in a 3 mm NMR tube 45 ...
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bioRxiv - Cancer Biology 2022Quote: ... the dried pellet was dissolved in 550 µl D2O containing 0.01 mg/ml Sodium 2,2-Dimethyl-2-Silapentane-5-Sulfonate (DSS; Cambridge Isotope). The samples were then vortexed and centrifuged at 16000 rpm for 12 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were thawed on ice and phthalates were extracted from a 10 μL aliquot via vigorous vortexing for 30 min at 4°C in the presence of 5:3:2 methanol:acetonitrile:water (240 μL) containing an isotope labeled standard (1 μM DEHP ring-1,2-13C2, Cambridge Isotope Laboratories). Extraction supernatants were clarified via centrifugation at 18,000 rpm for 10 min at 4°C and analyzed immediately by ultra high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) ...
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bioRxiv - Biochemistry 2021Quote: ... containing 0.11 mM of the internal standard DSS (sodium 2,2-dimethyl-2-silapentane-5-sulfonate, D6, 98%; Cambridge Isotope Laboratories) at pH 7.0 and vortex mixed for <1 min prior to transfer into 1.7 mm SampleJet NMR tubes ...