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Citations for Cambridge Isotope Labs :
151 - 200 of 277 citations for DL Lysine 2 15N DiHCl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Taurine concentrations were then measured via LC-MS and analyzed the area of the exact mass for the corresponding ions of the targets as well as the intracellular labeled isotopic taurine levels (13C2, 99%; 15N, 98%, Cambridge isotope #CNLM-10253-PK).
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were gently washed with PBS twice and switched into media containing the same components except for replacing light lysine with heavy stable isotope labeled (13C615N2) lysine (Cambridge Isotope Laboratories #CNLM-291-H-PK). For multiple time point experiments ...
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bioRxiv - Immunology 2021Quote: ... or heavy L-[13C6,15N2] lysine/L-[13C6,15N4] arginine (Cambridge Isotope Laboratories). CD8+ and CD4+ T effectors were stimulated by addition to tissue culture flasks coated with anti-CD3 (produced in-house ...
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bioRxiv - Microbiology 2021Quote: ... Medium (R6K4) or Heavy (R10K8) Arginine and Lysine (Cambridge Isotope Laboratories) for 5 passages ...
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bioRxiv - Cell Biology 2022Quote: ... 15N2 l-lysine (heavy SCD, Cambridge Isotope Laboratories, 25 mg/L).
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bioRxiv - Cell Biology 2022Quote: ... and 13C6 15N2 l-lysine (175 mg/liter) (Cambridge Isotope Laboratories). Experiments were conducted when incorporation rate of labelled amino acids was superior to 95% ...
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bioRxiv - Cell Biology 2022Quote: ... or L-arginine [13C6] and L-lysine [2H4] (Cambridge Isotope Laboratories).
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
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bioRxiv - Biochemistry 2023Quote: ... uniformly 15N-labeled and 13C/15N-labeled Cd-IF1 were over-expressed in M9 media with 15NH4Cl and 15NH4Cl/13C-glucose (Cambridge Isotope Laboratories, Inc. Andover, MA) using the high-yield protein expression protocol [11] and purified as previously described [12] ...
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bioRxiv - Physiology 2023Quote: ... three female FVB mice were fed a spirulina 15N-containg chow for the duration of the study (initial labeling, breeding, pregnancy, and weaning) (Cambridge Isotope Laboratories, Inc., Tewksbury, MA). After 13 weeks of being on a 15N diet ...
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µL of fluid was extracted in 45 µL of 80% methanol containing 500 nM 13C and 15N amino acid standards (Cambridge Isotope Laboratory, MSK-A2-1.2). Samples were vortexed for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4,5,5-[2H2]-L-Lysine (d4-L-Lys) was purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... or “heavy” versions of Lysine (115µM) and Arginine (200µM) (Cambridge Isotope Laboratories). The “light” (Agr0 ...
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bioRxiv - Molecular Biology 2024Quote: ... and heavy lysine (L-Lys-2HCl) (91.25 mg/L) (Cambridge Isotope Laboratories)17–19 ...
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bioRxiv - Microbiology 2021Quote: ... The cosms were surface-irrigated at 7 am with 60 ml of either 19.85 mM 15NH4Cl (99% 15N, Cambridge Isotope Laboratories, Inc., MA, US, pH 5.47) or a 19.98 mM 15KNO3 (99% 15N ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Evolutionary Biology 2020Quote: ... for “medium” (M) labeling and (3) 13C6 15N2 L-Lysine (Cambridge Isotope Laboratories) for “heavy” (H ...
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bioRxiv - Cell Biology 2021Quote: ... 15N2] lysine (100 mg/L) and [13C6,15N4] arginine (100 mg/L) (Cambridge Isotope Laboratories) (Heavy ...
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bioRxiv - Neuroscience 2020Quote: ... the mice were fed with SILAC L-Lysine (13C6) feed purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... or L-Arginine−13C6 and L-Lysine 4,4,5,5-2H4 (HMI11-R6K4) (Cambridge Isotope Laboratories) were added at 120 µM and 240 µM respectively (Cox et al. ...
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bioRxiv - Systems Biology 2020Quote: ... supplemented with L-arginine:HCl (13C6, 99%) and L-lysine:2HCl (13C6, 99%;Cambridge Isotope Laboratories) at concentrations of 0.13 g/l and 0.0904 g/l respectively ...
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bioRxiv - Biochemistry 2022Quote: ... For SILAC labeling “heavy” lysine (13C6, 15N2, Cambridge Isotope Laboratories Inc., CNLM-291-H-PK) and arginine (13C6 ...
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bioRxiv - Cell Biology 2020Quote: ... or L-Arginine U-13C6 and L-Lysine 4,4,5,5-2H4 (HMI11-R6K4) (Cambridge Isotope Laboratories) were added at 120 uM and 240 uM respectively ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... 181.2 mg/ml light or 13C615N2 lysine (Cambridge Isotope Laboratories, Inc., CNLM-291-H-1), and 87.8 mg/ml light or 13C615N4 arginine (Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... 84 mg/l 13C615N4 L-arginine and 175 mg/l 13C615N2 L-lysine (Cambridge Isotope Laboratories) until they were fully labeled ...
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bioRxiv - Biochemistry 2020Quote: ... or ‘heavy’ 13C615N2-Lysine (Silantes; 211604102) and 13C615N4-Arginine (Cambridge Isotope Labs Inc; CNLM-539-H-1). Cells were cultured in SILAC ‘light’ or ‘heavy’ media for two weeks prior to harvesting by centrifugation (800g ...
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bioRxiv - Microbiology 2021Quote: Heavy medium (HM) was prepared by adding 146 mg/mL [13C6,15N2]L-Lysine-2HCl (Cambridge Isotope Laboratories) and 84 mg/mL [13C6,15N4]L-Arginine-HCl (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... R0) or 0.80 mM [U-13C6,15N2] L-lysine and 0.40 mM [U-13C6] L-arginine (Cambridge Isotope Laboratories ...
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bioRxiv - Genomics 2024Quote: ... and heavy amino acid isotopes (L-lysine, +8Da; Cambridge Isotope Laboratories, #CNLM291H; L-arginine +10Da; Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... [13C6][15N4] L-arginine (Arg-10) and [13C6][15N2] L-lysine (Lys-8) were added (Cambridge Isotope Laboratories). Both forward (heavy CNOT1 siRNA/medium-heavy control siRNA ...
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bioRxiv - Neuroscience 2023Quote: ... and heavy isotopes (4, 4, 5, 5-D4 L-lysine and 13C6 L-arginine, Cambridge Isotope Laboratories (CIL)) for the K4/R6 population ...
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bioRxiv - Cell Biology 2024Quote: ... Cells to be grown in SD media containing heavy Lysine (13C6 15N2 (Cambridge Isotope Laboratories #CNLM-291-H) (25mg/L ...
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bioRxiv - Cancer Biology 2020Quote: ... Heavy lysine (1G: CLM-265-H-1) and arginine (1G: CNLM-291-H-1) were purchased from Cambridge Isotope Laboratories (CIL ...
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bioRxiv - Molecular Biology 2021Quote: ... a supplement of [13C6] L-arginine (Arg-6) and [2H4] L-lysine (Lys-4) was added (Cambridge Isotope Laboratories). For the heavy isotope containing medium ...
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bioRxiv - Immunology 2021Quote: ... and either L-Lysine-2HCl 13C6 15N2 and L-Arginine-HCl 13C6 15N4 (‘Heavy’ amino acids) or L-Lysine-2HCl 4,4,5,5-D4 and L-Arginine-HCl 13C6 (‘Medium’ amino acids) (all Cambridge Isotope Laboratories). Wt cells were grown in ‘Medium’ SILAC media and ifitm3-/- were grown in ‘Heavy’ SILAC media ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 84 mg/l 13C615N4 L-arginine and 175 mg/l 13C615N2 L-lysine (Cambridge Isotope Laboratories, Inc., Tewksbury, MA). All the cell lines were cultured under standard conditions (37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were prepared from E18-19 rat brains and grown on poly-D-lysine coated 15 cm plates at a density of 3.5 million cells/ 15 cm plate in DMEM media without l-arginine or l-lysine (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg”4”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories), and incubated for 2 hr as previously described elsewhere (Imami et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17) and L-Lysine [13C6,15N2]HCl (Lys-8) (Cambridge Isotope Laboratories). Cells were passaged and expanded in the corresponding SILAC media for 10 doublings.
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bioRxiv - Cancer Biology 2024Quote: ... prepared with either light or heavy amino acid isotopes (84 mg/L L-arginine:HCl [13C6,15N4], 175 mg/L L-lysine:2HCl [13C6,15N2], 100 mg/L L-leucine [13C6]; Cambridge Isotope Laboratories, Inc.), 44 mM sodium bicarbonate ...
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bioRxiv - Neuroscience 2024Quote: ... Heavy SILAC media was supplemented with L-Lysine:2HCl (13C6, 99%; 15N2, 99%) (Cambridge Isotope Laboratories, Inc., CNLM-291-H) and L-Arginine:HCl (13C6 ...
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bioRxiv - Neuroscience 2024Quote: ... On day 8, mice were switched to Mouse Express L-Lysine (13C6, 99%) irradiated mouse feed (Cambridge Isotope Laboratories, USA) for 8 days at libitum ...
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bioRxiv - Cancer Biology 2020Quote: ... 2mM were cultured with 2H4-lysine and 13C6-arginine (medium labelling) and 4mM were cultured with 15N2 13C6-lysine and 15N4 13C6-arginine (heavy labelling) (Cambridge Isotope Laboratory ...
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bioRxiv - Molecular Biology 2022Quote: ... medium (L-arginine:HCL [U13C6] and L-lysine:2HCL [4,4,5,5-D4], or heavy (L-arginine:HCL (13C6, 15N4) and L-lysine:2HCL (13C6, 15N2)) amino acid isotopes (Cambridge Isotope Laboratories Inc.).
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bioRxiv - Systems Biology 2024Quote: ... and either heavy (13C615N4 L-arginine or Arg10 an 13C615N2 L-lysine or Lys8) and light amino acids (Cambridge Isotope Laboratories) at 0.4 mmol/L and 0.8 mmol/L ...
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bioRxiv - Developmental Biology 2024Quote: ... L-arginine hydrochloride (13C6, 15N4) and L-lysine hydrochloride (13C6, 15N2) (Cambridge Isotope Laboratories CNLM-539-H and CNLM-291-H) were dissolved in PBS and kept at −80°C and were used at the final concentrations of 0.549 mM and 0.648 mM ...