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Citations for Akoya Biosciences :
151 - 189 of 189 citations for Streptavidin ZAP Antibody Kit goat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories) with TSA with Opal dyes (Akoya Biotech). Sections were mounted in Mowiol for microscopy (Roth ...
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bioRxiv - Cell Biology 2021Quote: Antibodies were purchased preconjugated from Akoya Biosciences or sourced from other vendors and conjugated in-house using the CODEX Conjugation Kit (Akoya Biosciences) or by Leinco Technologies ...
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bioRxiv - Immunology 2023Quote: ... and the signal was enhanced using TSA Plus CY3/CY5 detection kits (NEL744001KT and NEL705A001KT, Akoya Biosciences). Images were acquired using an inverted microscope (IX73 ...
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bioRxiv - Immunology 2022Quote: ... the HRP-coupled secondary anti-rat OmniMap antibody (Ventana Medical Systems) for 12 min and finally Opal 520 fluorophore (Akoya Biosciences, 1:100) at RT for 8 min ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... the antibody solution was washed off using PBST and the antibody was developed in dark for 45 minutes without agitation using a tyramide staining solution (TSA PLUS Fluorescein Reagent, Akoya Biosciences, TS-000200) diluted 1:50 in amplification buffer (1X Plus Amplification Diluent ...
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bioRxiv - Immunology 2021Quote: ... the RNAScope multiplex fluorescent kit V2 (Advanced Cell Diagnostics) was used with the opal fluorophores system (Akoya Bioscience) according to the manufacturer’s instructions and as described previously66 with some modifications ...
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bioRxiv - Cancer Biology 2020Quote: FFPE tissue sections were stained for multiplex fluorescence-based IHC using the opal 7-colour kit (Akoya Biosciences). Sections were dewaxed and rehydrated ...
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bioRxiv - Neuroscience 2021Quote: ... and TSA amplification diluent are part of the TSA detection kit (catalog no. NEL701A001KT, Akoya Biosciences, Marlborough, MA). Sections were washed 3 x 10 min in TNT buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorophore reaction was performed using OPAL 570 and OPAL 650 reaction kits for 10 min at RT (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: Multispectral analysis of FFPE tissue utilised the MOTiF™ PD-1/PD-L1 kit (Akoya Biosciences, cat# OP-000001). Staining with Leica BOND RX (Leica Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
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bioRxiv - Immunology 2022Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were blocked with the blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... CA) using probes against the leptin receptor mRNA (Cat# 415951) and Opal 650 dye kit (Akoya Biosciences, Marlborough, MA) to label LepR mRNA ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence staining of paraffin embedded tissues were processed using the OPAL 4-Color Manual IHC Kit (Akoya Biosciences, NEL810001KT).
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bioRxiv - Immunology 2024Quote: ... mIF was performed according to the manufacturer’s protocol using the OPAL Multiplex Manual IHC kit and antigen retrieval (AR) buffers AR6 and AR9 (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Multiplex immunofluorescence staining was performed with the Akoya Opal™ Polaris 7-color IHC kit fluorophores (Akoya Biosciences [NEL861001KT]). FFPE tissue sections were baked for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The procedure for multiplex immunohistochemistry (mIHC) was followed by a manufacturer’s protocol for Opal7-color automation IHC kit (Akoya Bioscience), and the staining was performed with Autostainer DISCOVERY ULTRA (Ventana) ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... Bound probes were detected using the RNAscope v2 multiplex fluorescent detection kit and Opal 520 and 690 Dyes (Akoya Biosciences).
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bioRxiv - Immunology 2022Quote: Oligonucleotide barcoded antibody staining of tissue sections mounted on cover slips (n=1 section per tissue region per donor) was performed using a commercially available CODEX Staining Kit according to the manufacturer’s instructions for FFPE tissue (Akoya Biosciences) and as recorded for HuBMAP Lymphatic System TMC SOPs (https://www.protocols.io/view/codex-antibody-staining-protocol-for-ffpe-tissues-bbsdina6) ...
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bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... the DIG-labeled probes were amplified with the Cy3 TSA reagent included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:500 in the same blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were washed for five minutes in 500 μL of the 1X amplification diluent included in the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Developmental Biology 2021Quote: ... FISH was performed as described (Brend and Holley 2009; Marra et al., 2017) using TSA Plus Fluorescein or Cyanine Kits (Akoya Biosciences). For all gene expression studies ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent RNA in situ hybridization was done with DIG-labeled probes for white and yellow with TSA Plus Fluorescence Kits (Akoya Biosciences) and used for in situ hybridization to imaginal discs as described (Tian et al ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2021Quote: ... the RNAscope probe for TNFR1 (product #408111) and the TSA Cyanine 3 Plus Evaluation kit (product #NEL744001KT, Akoya Biosciences, Boston, MA, USA). After completing the FISH protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then washed for 3 x 5 minutes in NTT and developed with Cy5-Tyramide signal amplification reagent (TSA Plus Kit, Akoya Biosciences #NEL745001KT) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Immunohistochemical staining for insulin and glucagon was conducted using a Tyramide Signal Amplification (TSA) Plus Biotin kit according to the manufacturer’s protocol (NEL700A001KT, Akoya Biosciences, Marlborough, MA, USA), as previously described [15] ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then rinsed through two rounds of incubation in distilled water for 2 minutes and stored in Hydration Buffer from the PhenoCycler Staining Kit (Akoya Biosciences, MA, #7000008) until ready for staining.
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bioRxiv - Developmental Biology 2022Quote: ... Signals were amplified using biotin/streptavidin HRP-conjugated secondary Ab (Jackson Immuno, 111-065-003 and 016-030-084) and detected using Tyramide Signal Amplification Plus kit (Akoya Biosciences, NEL744001KT, 1:100). Images were taken using a Leica SP5X laser scanning confocal microscope and further processed using ImageJ Fiji software (Schindelin et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were incubated in a 1:100 dilution of Cy3-Tyramide Signal Amplification Solution (TSA plus Cyanine 3 Kit, Akoya Biosciences, Marlborough, MA, USA) for 5 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: All antibodies used for multiplexed immunofluorescence were validated individually first by chromogenic singleplex immunohistochemistry (IHC) and then by singleplex immunofluorescence using the Opal 7 kit reagents (NEL871001KT, Akoya Biosciences, Waltham, MA, USA). Detailed methods and antibody information are in supplementary data ...